-
forward looking, lateral thinking
Improving Biorestoration of Impacted Sites Through Microbial
Profiling
(Metagenomics)
Monique Haakensen, PhD, PBiol, EP
Contango Strategies Ltd
Pittet, V., Haakensen, M., Majano, J.
-
forward looking, lateral thinking
Perceptions Google search for “microbiologist”
-
forward looking, lateral thinking
Community
-
forward looking, lateral thinking
Relationships
-
forward looking, lateral thinking
Perspective 1%
99%
Bacteria
10%
90%
Land animals
known
expected to exist
~10 Million
~100 Million
-
forward looking, lateral thinking
Challenge = opportunity
Source: scimaps.org
-
forward looking, lateral thinking
Brief overview of “omics”
DNA
gene
RNA protein
some function for the organism
gene-specific studies
transcriptomic
genomic
proteomic
metagenomic metatranscriptomic
-
forward looking, lateral thinking
DNA RNA
amplicon sequencing
whole genome shotgun sequencing
1) Who is there? (targeted)
transcriptome sequencing
2) Who is there and what might they be able to do?
3) What are they actually doing?
-
forward looking, lateral thinking
1) Who is there?
Amplicon Sequencing • Targeted • Bacteria • Archaea • Algae •
Fungi • Invertebrates • …???
Methylamine-enriched community of Lake Washington sediment
featuring Methylotenera cells. Photo © Dennis Kunkel Microscopy,
Inc. (Color by Ekaterina Latypova)
-
forward looking, lateral thinking
-
forward looking, lateral thinking
-
forward looking, lateral thinking
Key players
-
forward looking, lateral thinking
2) Who is there and what might they be able to do?
-
forward looking, lateral thinking
RNA extraction
Sequencing
Different levels of
gene expression
6x more in Lake 1 2x more in Lake 2 2x more in Lake 2 no
difference
Lake 2 Lake 1 Lake 1 Lake 2
3) What are they actually doing?
-
forward looking, lateral thinking
Ref. 1 Ref. 2 Ref. 3 Ref. 4 Imp. 1 Imp. 2
-
forward looking, lateral thinking
-
forward looking, lateral thinking
Applications
• What microbes/pathways are available for use?
• How are microbes responding to treatment?
• How is contamination affecting basic trophic levels?
• What can be done to improve treatment?
• Why are sites/treatments responding differently?
• Diversity indices
-
forward looking, lateral thinking
Thank-You
contangostrategies.com [email protected] Phone: 306
978 3111
Slide Number
1PerceptionsCommunityRelationshipsPerspectiveChallenge =
opportunityBrief overview of “omics”Slide Number 81) Who is
there?Slide Number 10Slide Number 11Key playersSlide Number 133)
What are they actually doing?Slide Number 15Slide Number
16ApplicationsThank-You
