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Implementation in Romania of the 2015 co-financed programme for control and monitoring of Classical Swine Fever National Sanitary Veterinary and Food Safety Authority SCoPAFF, 1 st –2 nd of June 2016 Brussels

Implementation in Romania of the 2015 co-financed ... › food › sites › food › files › animals › ... · Evolution 2011-2015 - Tested wild boars. ELISA FAT PCR 2011 7280

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Page 1: Implementation in Romania of the 2015 co-financed ... › food › sites › food › files › animals › ... · Evolution 2011-2015 - Tested wild boars. ELISA FAT PCR 2011 7280

Implementation in Romania of the 2015 co-financed programme

for control and monitoring ofClassical Swine Fever

National Sanitary Veterinary and Food Safety Authority

SCoPAFF, 1st – 2nd of June 2016Brussels

Page 2: Implementation in Romania of the 2015 co-financed ... › food › sites › food › files › animals › ... · Evolution 2011-2015 - Tested wild boars. ELISA FAT PCR 2011 7280

Content

DescriptionGeneral Objectives Evolution 2015SynthesisConclusion

Page 3: Implementation in Romania of the 2015 co-financed ... › food › sites › food › files › animals › ... · Evolution 2011-2015 - Tested wild boars. ELISA FAT PCR 2011 7280

General Objectives (1)

surveillance of the health status of domestic and wild pig population

demonstrate the absence of the CSF virus circulation

demonstrate freedom of CSF

Page 4: Implementation in Romania of the 2015 co-financed ... › food › sites › food › files › animals › ... · Evolution 2011-2015 - Tested wild boars. ELISA FAT PCR 2011 7280

Implementation of the programme

Target population – entire domestic and wild pig population of the country

Methods -Active and passive surveillance - by clinical inspections, serology and virology

surveillance

Page 5: Implementation in Romania of the 2015 co-financed ... › food › sites › food › files › animals › ... · Evolution 2011-2015 - Tested wild boars. ELISA FAT PCR 2011 7280

Pig population dynamic and distribution (1)Dynamic of the pig holdings 2008-2015

0

200

400

600

800

1000

1200

1400

1600

2008 2009 2010 2011 2012 2013 2014 2015

CommercialfarmsBackyards(thousands)

Page 6: Implementation in Romania of the 2015 co-financed ... › food › sites › food › files › animals › ... · Evolution 2011-2015 - Tested wild boars. ELISA FAT PCR 2011 7280
Page 7: Implementation in Romania of the 2015 co-financed ... › food › sites › food › files › animals › ... · Evolution 2011-2015 - Tested wild boars. ELISA FAT PCR 2011 7280

Pig population dynamic and distribution (2)Evolution of the number (thousands) of domestic

pigs 2008 - 2015

0500

100015002000250030003500400045005000

2008 2009 2010 2011 2012 2013 2014 2015

Commercial farmsBackyards

Page 8: Implementation in Romania of the 2015 co-financed ... › food › sites › food › files › animals › ... · Evolution 2011-2015 - Tested wild boars. ELISA FAT PCR 2011 7280

Evolution of Wild Boars population 2008-2015

0

1000020000

30000

4000050000

60000

7000080000

90000

2008 2009 2010 2011 2012 2013 2014 2015

Wild Boars

Page 9: Implementation in Romania of the 2015 co-financed ... › food › sites › food › files › animals › ... · Evolution 2011-2015 - Tested wild boars. ELISA FAT PCR 2011 7280

Serological Surveillance in backyard holdings 2015Active serological surveillance

First campaign: February-May – blood samples from all breeding sows

Second campaign: June-July - randomely sampling of locality, herds and animals taking into consideration a prevalence of 5% with 95% confidence

A third campaign: November - randomely sampling of animals taking into consideration a prevalence of 0,5% with 95% confidence from each chosen locality

Due to financial constraints the 3rd -active surveillance campaign was not performed

Passive surveillanceFrom all aborted sow

From ill pigs with clinical signs that can also be seen in CSF

Page 10: Implementation in Romania of the 2015 co-financed ... › food › sites › food › files › animals › ... · Evolution 2011-2015 - Tested wild boars. ELISA FAT PCR 2011 7280

Virological surveillance in backyards holdings 2015

RT- PCR on- organs from dead pigs with lesions that can be seen

also in CSF- blood from aborted sow- blood from ill pig with clinical signs that can be seen

also in CSF- avortons

Page 11: Implementation in Romania of the 2015 co-financed ... › food › sites › food › files › animals › ... · Evolution 2011-2015 - Tested wild boars. ELISA FAT PCR 2011 7280

Serological surveillance in commercial farms 2015

Active surveillance- randomely sampling of fattening pigs, with a prevalence

of 5% and 95% confidence in June-July

Passive surveillance- From all aborted sow- From all ill pigs with clinical signs that can be seen also in

CSF

Page 12: Implementation in Romania of the 2015 co-financed ... › food › sites › food › files › animals › ... · Evolution 2011-2015 - Tested wild boars. ELISA FAT PCR 2011 7280

Virological surveillance in Commercial farms 2015

RT- PCR on

- organs from dead pigs with lesions that can be seen also in CSF- organs from dead pigs when the dead pigs is above technological

mortality- blood from aborted sow- blood from ill pig with clinical signs that can be seen also in CSF- avortons

Page 13: Implementation in Romania of the 2015 co-financed ... › food › sites › food › files › animals › ... · Evolution 2011-2015 - Tested wild boars. ELISA FAT PCR 2011 7280

Surveillance in wild boars-serological surveilance using ELISA on all hunted wild boars

-virological surveilance using RT-PCR and FAT of allhunted and found dead wild boar

Page 14: Implementation in Romania of the 2015 co-financed ... › food › sites › food › files › animals › ... · Evolution 2011-2015 - Tested wild boars. ELISA FAT PCR 2011 7280

Active surveillanceActive surveillance237153 animals tested by ELISA237153 animals tested by ELISA

CampaignCommercial

holdings(no of tested animals)

BackyardHoldings

(no of tested animals)

February - May89373sows

June - July 39200fattening pigs

108580fattening pigs

TOTAL 39200 197953

Page 15: Implementation in Romania of the 2015 co-financed ... › food › sites › food › files › animals › ... · Evolution 2011-2015 - Tested wild boars. ELISA FAT PCR 2011 7280

Passive surveillance backyards holdings 2015

Year Animals Samples

ELISA PCR FAT

Serum Blood EDTA Org Fetus Sternum

2015 5619 7193 154 4637 2360 36 6

Page 16: Implementation in Romania of the 2015 co-financed ... › food › sites › food › files › animals › ... · Evolution 2011-2015 - Tested wild boars. ELISA FAT PCR 2011 7280

Passive surveillance commercial holdings2015 at December 31

Year Animals tested Tests

Sows Sick Dead Fetus ELISA PCR

2015 1150 2374 2663 116 1202 6205

Page 17: Implementation in Romania of the 2015 co-financed ... › food › sites › food › files › animals › ... · Evolution 2011-2015 - Tested wild boars. ELISA FAT PCR 2011 7280

Wild boars surveillance 2015

Wild boars Animals testedin 2015

Elisa FAT RT-PCR

TOTAL 19915 19453 19862 15320

Page 18: Implementation in Romania of the 2015 co-financed ... › food › sites › food › files › animals › ... · Evolution 2011-2015 - Tested wild boars. ELISA FAT PCR 2011 7280

.

Evolution 2011-2015 - Tested wild boars

ELISA FAT PCR2011 7280 (90 positive*) 10318 all negative 2016 all negative

2012 6191 (33 positive*) 10884 all negative 10264 all negative2013 9492 (6 positive*) 14477 all negative 9278 all negative2014 14259 (6 positive*) 16521 all negative 8135 all negative2015 19453 (11 positive*) 19862 all negative 15320 all negative

0

2000

4000

6000

8000

10000

12000

14000

16000

18000

20000

ELISA 0

2000

4000

6000

8000

10000

12000

14000

16000

PCR

0

2000

4000

6000

8000

10000

12000

14000

16000

18000

20000

FAT

20112012201320142015

* Old vaccinated animals

Page 19: Implementation in Romania of the 2015 co-financed ... › food › sites › food › files › animals › ... · Evolution 2011-2015 - Tested wild boars. ELISA FAT PCR 2011 7280

Costs of CSF program -2015TOTAL COST

Sampling

COST of

Tests

Domestic Pigs

COST of

Tests

Wild Boars

TOTAL COST

Tests

TOTAL COST

General

527533 EUR 899,661.73

EUR

250765,76

EUR

1150427,49 EUR

1,677,960.49 EUR

1,089,525 backyards inspected in 2015 by empowered private vets for the clinical inspections of pigs ≈ 6 millions EUR

Cost of inspections are not co-financed by European Commission

Page 20: Implementation in Romania of the 2015 co-financed ... › food › sites › food › files › animals › ... · Evolution 2011-2015 - Tested wild boars. ELISA FAT PCR 2011 7280

Final remarksThe last cases of CSF reported from Romania in domestic or feral pigs date back to 2007.

Romania is still listed in the Annex to Commission implementing Decision 2013/764/EU concerning animal health control measures relating to CSF in certain Member States (Mss).

Programmes implemented since 2012 with intense active and passive surveillance on unvaccinated animals demonstrated the absence of the CSF virus. This entitles Romania to be considered as free of CSF.

Page 21: Implementation in Romania of the 2015 co-financed ... › food › sites › food › files › animals › ... · Evolution 2011-2015 - Tested wild boars. ELISA FAT PCR 2011 7280

Thank you for your Thank you for your attention !attention !