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ROITT'S ESSENTIAL IMMUNOLOGY 12th Edition (All MCQs With
Answers)
Chapter 1
1 Which of the following does not protect body surfaces:
A Skin.
B Mucus.
C Gastric acid.
D Salivary amylase
E Gut microflora.
2 Pattern recognition receptors (PRR) include:
A LPS.
B PAMPs.
C Lipoteichoic acid.
D Lectin-like molecules.
E Unmethylated CpG sequences.
3 The mononuclear phagocyte system does not include:
A Monocytes.
B Kupffer cells.
C Kidney mesangial cells.
D Lymph node medullary macrophages.
E Endothelial cells.
4 A polymorphonuclear neutrophil (PMN):
A Is a bone marrow stem cell.
B Is closely similar to a mast cell.
C Contains microbicidal cytoplasmic granules.
D Is not a professional phagocytic cell.
E Has granules which stain with eosin.
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5 Which of the following is not produced following activation of
the NADPH oxidase microbicidal pathway
A O2
B O2
C H2O2
D NO
E OH
6 Neutrophil defensins are:
A Anti-toxins.
B Oxygen-dependent.
C Enzymes.
D Glycolipids.
E Peptide antibiotics.
7 The TLR9 pattern recognition receptor recognises:
A CpG motifs.
B Gram +ve peptidoglycan.
C Mycobacterial lipoarabinomannan.
D Gram ve LPS.
E dsRNA.
8 Complement component C3 is cleaved by:
A C3b
B C3bBb
C Factor B
D Factor D
E Factor H
9 The membrane attack complex consists of:
A OH.
B Colicins
C C3b3b,Bb
D C5b,6,7,8,9
E Properdin
10 C3b:
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A Is chemotactic.
B Is an anaphylatoxin.
C Opsonizes bacteria.
D Directly injures bacteria.
E Is the inactive form of C3.
11 Acute inflammation characteristically involves:
A Constriction of arterioles.
B Capillary endothelial cell enlargement.
C Influx of macrophages.
D Influx of mast cells.
E Influx of neutrophils.
12 Lysozyme:
A Is a cytoplasmic organelle.
B Activates complement.
C Is a proteolytic enzyme.
D Splits peptidoglycan.
E Is released by mast cells.
13 Which of the following is not an acute phase protein:
A Serum amyloid P component.
B Chondroitin sulfate.
C C-reactive protein.
D Mannose binding lectin.
E Fibrinogen.
14 Interferons:
A Are found only in mammalian species.
B Are divided into 5 main families.
C Induce enzyme synthesis in the target cell.
D Only affect infected cells.
E Are specific for individual viruses.
15 Natural killer (NK) cells do not:
A Respond to interferon.
B Contain perforin.
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C Contain tumor necrosis factor (TNF).
D Kill only by damaging the target cell outer membrane.
E Contain serine proteases.
16 Eosinophils do not:
A Stain with basic dyes.
B Contain a major basic protein.
C Contain peroxidase.
D Give a respiratory burst on activation.
E Have C3b receptors.
17 Polymorphonuclear neutrophils attack bacteria:
A Exclusively by oxygen-dependent mechanisms.
B Exclusively by oxygen-independent mechanisms.
C By phagocytosis.
D By secreting complement.
E By secreting interferon.
18 A complement component which is strongly chemotactic for
neutrophils is:
A C9
B C5a
C C3
D C3b
E C5b
19 Acute inflammation can be initiated by:
A Mast cell activation.
B Influx of neutrophils.
C An increase in vascular permeability.
D C3.
E Lysozyme.
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Chapter 2
1 The initial complement component that is bound by
complement-fixing antibodies is:
A C1q
B C1s
C C3b
D C5a
E C9
2 Several of the complement components are:
A Glycolipids
B Cytokines
C Enzymes
D Hormones
E Antibodies
3 The classical and alternative pathways meet at complement
component:
A C4
B C4b
C Factor D
D C5
E C3
4 Clonal selection occurs when antigen is encountered by:
A Neutrophils
B Mast cells
C T-cells
D Basophils
E Eosinophils
5 Plasma cells:
A Have a thin layer of cytoplasm
B Are derived from T-cells
C Develop into B-cells
D Secrete large amounts of gamma interferon
E Have a highly developed rough endoplasmic reticulum
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6 Specific antibodies are readily detectable in serum following
primary contact with antigen after:
A 10 min
B 1 h
C 57 days
D 35 weeks
E Only following a second contact with antigen
7 A plasma cell secretes:
A Antibody of a single specificity related to that on the
surface of the parent B-cell
B Antibody of two antigen specificities
C The antigen it recognizes
D Many different types of antibody
E Lysozyme
8 Adoptive transfer of acquired immune responsiveness involves
the transfer of:
A Antibody
B Complement
C Phagocytes
D Lymphocytes
E Serum
9 The main reason an experimental animal treated with X-rays can
act as a living test tube for lymphocyte transfer experiments is
because:
A It is microbiologically sterile
B Complement components will be inactivated
C The host lymphocytes are destroyed or unable to divide
D Only non-dividing cells are affected
E The requirement for T-cell help is overcome
10 Immunological unresponsiveness to self antigens is
called:
A Tolerance
B Tolerogen
C Memory
D Acquired immunity
E ADCC
11 Edward Jenner vaccinated against smallpox using:
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A Killed smallpox virus
B A recombinant protein derived from smallpox
C An unrelated virus
D Toxoid
E Cowpox
12 Protective antibodies against infectious agents are
often:
A Autoantibodies
B Neutralizing
C Toxoids
D Natural Killer
E Non-specific
13 Intracellular parasites within macrophages are killed more
readily in the presence of:
A Antibody
B Kinins
C Properdin
D Gamma-interferon
E Anaphylatoxin
14 T cell surface receptors for antigen partly recognize:
A Cytokines
B MHC
C ADCC
D Antibody
E IL-2
15 An immune response against grass pollen often involves:
A Pathogen-associated molecular patterns
B Breakdown of self tolerance
C A hypersensitivity reaction
D Reaction against MHC
E Persistent infection by the pollen
16 Secondary antibody responses are better because:
A They provide defense against unrelated antigens
B The antibody can be made by both T and B cells
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C Complement-fixing antibodies are made
D They do not require T-cell help
E They are stronger and faster
17 Which cell type produces antibodies?:
A Macrophages
B T-lymphocytes
C NK
D Plasma cells
E Eosinophils
18 Clonal selection occurs when a B-lymphocyte encounters:
A Cytokines
B Antigen
C T-lymphocytes
D Complement
E Chemotactic factors
19 The secondary, but not the primary, immune response is based
on:
A Memory
B The bonus effect of multivalency
C Complement activation
D Mast cell degranulation
E Clonal selection
20 Protection against microorganisms inside cells is provided
by:
A T-cells
B Antibody
C C3b
D C1q
E The membrane attack complex
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Chapter 3
1 The basic Ig unit is composed of:
A 2 identical heavy and 2 identical light chains.
B 2 identical heavy and 2 different light chains.
C 2 different heavy and 2 identical light chains.
D 2 different heavy and 2 different light chains.
E Non-covalently bound polypeptide chains.
2 A Fab fragment:
A Is produced by pepsin treatment.
B Is produced by separation of heavy and light chains.
C Binds antigen.
D Lacks light chains.
E Has no interchain disulfide bonds.
3 The complementarity determining regions:
A Are restricted to light chains.
B Are in the constant part of the Ig molecule.
C Bind to Fc receptors.
D Are concerned in antigen recognition.
E Occur at the C-terminal end of the Ig peptide chains.
4 Which of the following gene clusters do not contribute to
antigen binding:
A VL
B CL
C VH
D D
E J
5 Recombination of V, D and J Ig gene segments:
A Only occurs in mature B-cells.
B Only occurs in light chains.
C Involves heptamer-spacer-heptamer flanking sequences.
D Does not occur until the mRNA stage.
E Is effected by recombinase enzymes.
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6 Ig idiotypes are found:
A In the constant region of the heavy chain.
B In the constant region of the light chain.
C In the hinge region.
D In the variable region of both heavy and light chains.
E Only in the light chain.
7 With reference to the variable Ig domain, which of these
answers is false?:
A It mediates the secondary consequences of antigen
recognition.
B It has anti-parallel beta-pleated sheet structures.
C It uses beta-turn loops to bind antigen.
D It has an extra long beta-turn relative to constant region
domains.
E It has a typical Ig fold with an intra-chain disulfide
bond.
8 Which of the following statements does not apply to IgG?:
A Appears early in the primary immune response.
B Neutralizes bacterial toxins.
C Can fix complement.
D Crosses the human placenta.
E Opsonizes bacteria.
9 The low affinity Fc gamma RII IgG receptor:
A Has a GPI anchor.
B Binds monomeric IgE.
C Avidly binds monomeric IgG.
D Binds aggregated IgG.
E Is not present on macrophages.
10 IgA in seromucus secretions:
A Has no J-chain.
B Has no secretory piece.
C Is dimeric.
D Cannot bind to neutrophils.
E Activates the classical complement pathway.
11 IgM:
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A Is usually of high affinity.
B Is most commonly tetrameric.
C Has the same number of constant domains as IgG.
D Is a weak bacterial agglutinator.
E Is the main class of the 'natural antibodies'.
12 IgD:
A Is pentameric.
B Is resistant to proteolytic degradation.
C Is present mainly as a surface receptor on B-cells.
D Is present with unusual frequency in myelomas.
E Is abundant in milk
13 IgE:
A Is abundant in saliva.
B Binds strongly to mast cells.
C Cannot bind to macrophages.
D Activates the complement cascade.
E Has an insignificant role in worm infestations
14 The Fc receptor with the highest affinity of the following
five receptors is:
A CD64.
B FcgRII.
C CD16.
D FceRI.
E CD23
15 Cleavage of IgG by papain produces:
A Divalent antigen binding fragments.
B Isolated light chains.
C Isolated heavy chains.
D F(ab')2.
E Fab.
16 A given myeloma protein:
A Has a homogeneous amino acid structure.
B Has diverse variable polypeptide segments.
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C Has a constant heavy chain but both kappa and lambda light
chains.
D Is produced by different plasma cell clones.
E Does not behave as an antibody.
17 What is the approximate number of D segment genes in the
immunoglobulin heavy chain gene locus:
A 40
B 30
C 9
D 6
E 1
18 RAG-1 and RAG-2 enzymes effect the recombination of:
A VDJ to CH
B H to L
C CDR1 to CDR2
D V to D
E VJ to Cl
19 A given Ig isotype is:
A A heavy chain variant encoded by allelic genes.
B A light chain constant region encoded by allelic genes.
C Present in all normal individuals.
D A collection of hypervariable region epitopes recognized by an
anti-idiotype.
E Monoclonal.
20 The Fab region of an Ig is responsible for:
A C1q fixation.
B Binding to antigen.
C Binding to Fc receptors.
D Binding to macrophages.
E The ability of Ig to cross the human placenta.
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Chapter 4
1 The first immunoglobulin heavy chain class to be expressed on
the surface of a newly produced B-cell is:
A IgA
B IgD
C IgE
D IgG
E IgM
2 A B-cell is able to make cell-surface and secreted versions of
antibody using:
A Different gene pools.
B Differential splicing.
C Different heavy chain class but the same light chain.
D Different light chain class but the same heavy chain.
E F(ab')2 fragments.
3 The cytoplasmic region of surface IgM consists of:
A A single H chain constant region domain.
B A light chain.
C 110 amino acids.
D 3 amino acids.
E Carbohydrate.
4 The percentage of human peripheral blood T-cells bearing a
gamma delta T-cell receptor is:
A 3080%.
B 1-5%.
C 100%.
D 0%.
E Only present during mycobacterial infections.
5 The T-cell receptor genes were originally identified
using:
A A monoclonal anti-idiotype.
B The polymerase chain reaction.
C A liver DNA gene library.
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D In situ hybridization.
E Subtractive hybridization.
6 A chromosome on which T-cell receptor alpha chain gene
rearrangement has occurred lacks which of the following gene
segments:
A Joining (J).
B Diversity (D).
C Variable (V).
D Constant (C).
E TCR beta chain.
7 Using only random VDJ recombination, from 40 V, 30 D and 6 J
gene segments, the number of possible variable regions of an
antigen receptor molecule would be:
A 40
B 76
C 7,200
D 1.4 106
E 1.4 109
8 N-region insertion is associated with the expression of:
A Terminal deoxynucleotidyl transferase.
B NK cell antigen receptors.
C The proteasome.
D Lysozyme.
E Heat shock proteins.
9 Receptor editing:
A Has been described for antibody but not TCR.
B Is associated with reexpression of RAG-1 in germinal
centers.
C Can involve V genes which are 3' of the initially selected V
gene.
D Involves the S (switch) sequences.
E For B-cells only occurs in bone marrow.
10 Somatic hypermutation of V genes:
A Is confined to the hypervariable regions.
B Involves heavy but not light chain immunoglobulin V genes.
C Does not occur in IgM antibodies.
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D Can decrease the affinity of an antibody.
E Is commonly seen for both antibody and TCR.
11 NK cells lack receptors with:
A Specificity for MHC class II molecules.
B Specificity for MHC class I molecules.
C C-type lectin domains.
D Immunoglobulin-like domains.
E Specificity for the Fc region of IgG.
12 The Ly49A receptor on NK cells does not:
A Exist as a dimer.
B Possess an a-helix in its structure.
C Recruit phosphatases
D ITAMS.
E ITIMS.
13 The MHC class I heavy chain consists of:
A Beta2-microglobulin.
B Three Ig-type domains.
C A truncated MHC class II heavy chain.
D Three globular domains.
E Two globular domains.
14 The MHC class II beta chain has a molecular weight of:
A 2829 kDa
B 34 kDa
C 4344 kDa
D 1112 kDa
E 2425 kDa
15 The MHC class II region genes in the mouse are located:
A On a different chromosome to the class I region genes.
B On a different arm, but on the same chromosome as the class I
region genes.
C On chromosome 6.
D Adjacent to a class I gene.
E In the HLA locus.
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16 MHC class III genes encode:
A Complement component C3.
B Tumor necrosis factor.
C IL-2.
D Beta2-microglobulin.
E HLA-DQ.
17 Extensive allelic polymorphism is found in MHC:
A DRbeta.
B DRalpha.
C beta2-microglobulin.
D Class I alpha3 domain.
E Class II beta2 domain.
18 Expression of MHC genes is:
A Codominant.
B Dominant for maternal genes.
C Dominant for paternal genes.
D Dependent on thymic selection.
E Totally dependent on the antigenic exposure of the
individual.
19 The molecules mediating signal transduction following antigen
binding to cell surface immunoglobulin on a B-cell are called:
A Ig Fc
B Ig-alpha and Ig-beta
C MHC
D CD4
E CD8
20 The T-cell receptor for antigen is:
A Derived from the immunoglobulin gene pool by alternative
splicing.
B A tetramer.
C A homodimer.
D A heterodimer.
E A single chain molecule.
21 Each chain of the T-cell receptor consists of:
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A An Ig heavy chain.
B Two Ig-type domains.
C A fibronectin-type domain.
D Glycolipid molecules.
E Four Ig-type domains.
22 The T-cell receptor antigen recognition signal is transduced
by:
A The TCR alpha chain.
B The TCR beta chain.
C CD1.
D CD2.
E CD3.
23 MHC class II molecules are found on:
A Virtually all cells in the body.
B B cells, dendritic cells and macrophages.
C Only gamma-interferon activated cells.
D Virtually all nucleated cells in the body.
E Only on virally-infected cells.
24 Somatic hypermutation is:
A Commonly found in both Ig and T-cell receptor genes.
B Restricted to the constant region.
C Restricted to the hypervariable regions.
D Found only in Ig heavy chains.
E Found only in Ig variable regions.
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Chapter 5
1 A hapten is:
A An epitope.
B A paratope.
C A small chemical grouping which reacts with preformed
antibodies.
D A carrier.
E An immunogen.
2 A discontinuous antigen epitope is:
A Presented by MHC molecules.
B Usually concave.
C Representative of only a minority of B-cell epitopes.
D Produced by a continuous linear peptide sequence.
E Produced by amino acid residues on non-adjacent polypeptide
sequences.
3 Binding of antigen to antibody:
A Is usually unaffected by molecular rigidity.
B Is unaffected by the presence or absence of water
molecules.
C Involves covalent bonding.
D Is optimized by spatial complementarity.
E Is usually unaffected by pH.
4 The intermolecular forces which contribute to the interaction
between antibody andantigen:
A Are all electrostatic.
B Are all van der Waals.
C Are all hydrophobic.
D Are all hydrogen bonds.
E Rely on a combination of the above.
5 Which of the following statements is incorrect? Affinity
is:
A A measure of the strength of the binding of antigen to
antibody.
B The association constant of the Ag/Ab equilibrium.
C Avidity.
D Related to the free energy change of the Ag/Ab
interaction.
E Related to specificity.
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6 The antigen moiety on an antigen-presenting cell recognized by
the alpha beta T-cell receptor is:
A Native protein antigen plus major histocompatibility complex
(MHC) molecule.
B Processed (peptide) antigen plus MHC.
C Processed peptide antigen.
D Native antigen.
E MHC alone.
7 The processing of cytosolic protein involves:
A Transport into late endosomes.
B Proteasome-mediated cleavage.
C Displacement of invariant chain.
D Displacement of beta2-microglobulin.
E Binding to the MHC class II groove.
8 Which of the following statements does not apply to the
immunoproteasome:
A It is generated in response to interferon-gamma
stimulation.
B It contains the nonpolymorphic MECL1 subunit.
C It contains the polymorphic LMP7 subunit.
D Ten per cent of the peptides generated are 89 residues in
length.
E It exhibits both chymotrypsin-like and trypsin-like
activities.
9 Antigen processing for presentation by MHC class II molecules
involves:
A DM
B LMP2
C TAP1 and TAP2
D Calnexin
E Proteasomal-mediated cleavage
10 The processed peptide binding to the MHC class I groove:
A Is usually more than 11 amino acids long.
B Hangs over the ends of the groove.
C Usually binds to the groove through 2 anchor residues.
D Is mainly recognized by the CDR2 of the T-cell receptor
chains.
E Is derived from exogenous protein taken in by endocytosis.
11 TCR recognition of peptide-MHC class II depends on:
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A Covalent binding.
B Very high affinity interactions.
C CDR-mediated binding.
D A minimum of 2 peptides occupying the binding groove of each
MHC molecule.
E The presence of beta2 microglobulin.
12 Cross-presentation of exogenous antigen to a b T-cells does
not require theinvolvement of:
A Peptide-MHC recognition by the T-cell receptor.
B Antigen-processing
C MHC class I
D MHC class II
E An antigen-presenting cell.
13 An example of a 'nonclassical' MHC molecule is:
A H-2 A
B HLA-C
C H-2 L
D H-2E
E H-2 m
14 CD1:
A Is encoded in the MHC region.
B Structurally is most similar to MHC class II molecules.
C Can present antigens to gamma delta, but not alpha beta,
T-cells.
D Can present lipid antigens.
E Is encoded by a single gene.
15 NKT cells:
A Bear diverse TCR alpha chains.
B Have a limited TCR beta chain repertoire.
C Comprise 23% of T-cells in the mouse liver.
D Following stimulation secrete gamma interferon but not
IL-4.
E Are restricted by CD1 but not by classical MHC molecules.
16 Alkylamine antigens:
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A Are produced by Listeria monocytogenes.
B Are particularly recognized by V alpha9 V beta2 T-cells.
C Comprise a family of lipid antigens.
D Do not occur naturally.
E Stimulate alpha beta but not gamma delta T-cells.
17 Superantigens:
A Do not cause pathology.
B Are not mitogenic for T-cells.
C Bind to MHC class III.
D Bind to all members of a given V beta T-cell receptor
family.
E Have to be processed before recognition by the T-cell.
18 Alpha beta T-cells recognizing MHC plus processed peptide
can:
A Themselves produce antibody to directly eliminate
extracellular organisms.
B Release histamine.
C Scavenge unwanted metabolic products.
D Directly kill viruses.
E Recognize an intracellular infection.
19 An epitope:
A Is the area on an antigen which contacts antibody.
B Is the area on an antibody which contacts antigen.
C Requires both antigen-binding arms of the antibody molecule
for its recognition.
D Is usually composed of a linear sequence of amino acids.
E Is usually associated with a concave region of the
antigen.
20 The binding of antigen to antibody:
A Is irreversible.
B Depends on covalent interactions.
C Occurssolely by hydrophobic bonding.
D Depends on spatial complementarity.
E Is always of high affinity.
21 Alpha beta T-cell receptors recognize:
A Native antigen.
B Free linear antigenic peptide.
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C MHC beta2-microglobulin.
D Linear antigen peptide in the MHC groove.
E Lipid and glycolipid antigens associated with the MHC.
22 Peptides produced by processing of cytosolic proteins
largely:
A Are generated in late endosomal vacuoles.
B Enter the endoplasmic reticulum by diffusion.
C Are presented at the cell surface with MHC class II to CD4
T-helpers.
D Are presented at the cell surface with MHC class II to CD8
cytotoxic T-cells.
E Are presented at the cell surface with MHC class I to CD8
cytotoxic T-cells.
23 Antigenic peptides in the MHC class I groove:
A Are usually over 12 residues in length.
B Are usually under 12 residues in length.
C Extend beyond the groove.
D Usually have 3 or more invariant anchor residues.
E Do not vary at non-anchor residues.
24 Staphylococcus aureus superantigens:
A Do not cause pathology.
B Are recognized by T-cells in processed form.
C Are potent T-cell mitogens.
D Stimulate T-cells independently of V beta family.
E Stimulate T-cells independently of the presence of MHC class
II accessory cells.
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Chapter 6
1 In extreme antigen excess, immune complexes between IgG and a
tetravalent antigen have the composition:
A Ag1Ab4
B Ag4Ab3
C Ag2Ab1
D Ag3Ab2
E Ag2Ab7
2 Antibody titer refers to the:
A Absolute amount of specific antibody.
B Affinity of specific antibody.
C Avidity of specific antibody.
D Concentration of specific antibody.
E Highest dilution of antibody still able to give a positive
result in a test system.
3 The association constant (Ka) at equilibrium is represented
by:
A [AgAb complex]
B [free Ag][free Ab]
C [free Ag][free Ab]/[AgAb complex]
D [AgAb complex]/[free Ag][free Ab]
E [free Ag]/[free Ab]
4 The affinity of an antibody can be determined by
measuring:
A Its concentration.
B The valency of antigen binding.
C The amount of antibody bound at various antigen
concentrations.
D Its ability to neutralize bacterial toxins.
E The sedimentation coefficient of the antibody.
5 Latex particles are often used in:
A Agglutination tests.
B Affinity chromatography.
C Affinity measurements.
D Adjuvants.
E Neutralization assays.
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6 The RAST measures:
A Antigen concentration.
B IgE antibodies.
C IgM antibodies.
D Agglutination.
E IgG antibodies.
7 Surface plasmon resonance is a system based on:
A Fluorescence.
B Radioactivity.
C Reflected light.
D Molecular resonance.
E Magnetic fields.
8 Characterization of antigens by electrophoresis and
immunofixation relies on the reaction of antigen and antibody in
(or on):
A Agar.
B Streptavidin.
C Gold-plated sensor chip.
D Latex particles.
E Plastic microtiter plates.
9 Western blots are primarily used to detect:
A Protein.
B Carbohydrate.
C Lipid.
D RNA.
E DNA.
10 SDS-PAGE separates proteins on the basis of:
A Isoelectric point.
B Sedimentation coefficient.
C Amino acid sequence.
D Degree of glycosylation.
E Size.
11 The pepscan technique is most useful for determining:
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A Antibody structure.
B Discontinuous epitopes recognized by antibodies.
C Epitopes recognized by T-cells.
D MHC haplotypes.
E TCR V beta usage.
12 HAT medium is used to:
A Immortalize B-lymphocytes.
B Culture B-lymphocytes.
C Select for hybrids in the hybridoma technique.
D Kill B-cell hybridomas.
E Fuse B-lymphocytes to myeloma cells.
13 A HAMA response cannot be reduced or eliminated using:
A ADEPT.
B Chimeric antibodies.
C Humanized antibodies.
D Grafted CDRs.
E Human antibodies.
14 Human monoclonal antibodies can be obtained:
A Using Epstein-Barr virus immortalization of T-cells.
B Easily from human hybridomas selected with HT medium.
C Using transgenic xenomouse strains.
D By a single point mutation of a mouse monoclonal antibody.
E Only by fusing human B-cells with mouse myeloma cells.
15 A single chain Fv fragment (scFv) will:
A Fix C1q.
B Bear idiotypes.
C Cross-link antigen.
D Only be present on the surface of filamentous
bacteriophage.
E Possess either VH or VL but not both.
16 In affinity chromatography the required ligand is often
released by:
A Changing the pH
B Vigorous shaking
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C Changing the temperature from 37 to 4.
D Boiling.
E Adding a small amount of detergent (e.g. 0.025% Tween 20).
17 Coating a cell with specific antibody facilitates its
selection by:
A Sedimentation rate.
B Buoyant density.
C Adherence to plastic surfaces.
D Magnetic beads coated with anti-Ig.
E Light scatter in the fluorescence activated cell sorter
(FACS).
18 A T-cell hybridoma
A Is obtained by continuous culture of T-cells with antigen.
B Is obtained by cloning antigen-specific splenic T-cells.
C Is obtained by fusing T-cells with a T-cell tumor cell line
and cloning.
D Expresses a multiplicity of T-cell receptor specificities.
E Results from producing a mouse which is transgenic for the
rearranged alpha- and beta-receptor from a T-cell clone
19 Which of the following is not used as a direct conjugate to
the antibody for visualizing tissue antigens:
A Fluorescein.
B Anti-immunoglobulin.
C Alkaline phosphatase.
D Peroxidase.
E Gold particles.
20 A confocal microscope at high magnification does not:
A Bring all focal planes of an image into focus
simultaneously.
B Permit construction of a 3-dimensional image.
C Improve the quality of image definition in a single plane.
D Provide quantitative data.
E Function with fluorescent conjugates.
21 The use of green fluorescent protein in flow cytofluorimetry
permits measurement of:
A Cell size.
B Cell granularity.
C Antibody secretion.
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D DNA content.
E Intracellular gene expression.
22 The functional activity of neutrophils can be assessed
by:
A The nitroblue tetrazolium test.
B Proliferation.
C Limiting dilution analysis.
D A plaque test for antibody.
E A fluorescent antibody test for myeloperoxidase.
23 The activation of lymphocytes cannot be assessed by:
A Mitosis.
B Cytokine release.
C Phagocytosis.
D Cytotoxicity.
E Limiting dilution analysis.
24 The TUNEL technique can be employed to measure:
A Precursor frequency.
B Apoptosis.
C Directional migration of neutrophils.
D Phagocytosis.
E Surface exposed phosphatidylserine.
25 Antibody-secreting cells can be enumerated by:
A Mitosis.
B Cytokine release.
C Total antibody content.
D Cell surface immunoglobulin.
E ELISPOT.
26 Introduction of a gene into a cell using calcium phosphate
precipitate is termed:
A Electroporation.
B Homologous recombination.
C Biolistics.
D Transfection.
E Mutation.
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27 Common approach to generating conditional knockout mice
involves using:
A TdT.
B Radiation chimeras.
C ELISPOTS.
D Tritiated thymidine.
E LoxP.
28 A catalytic antibody is:
A An enzyme used to digest antibody.
B An immunoabsorbent.
C Found in the vacuoles of phagocytic cells.
D An antibody that is capable of acting as an enzyme.
E An antibody to zymosan.
29 In an ELISA you might use an antigen or antibody labeled
with:
A 125I
B FITC
C Colloidal gold
D Europium 3+
E Horseradish peroxidase
30 Which of the following is not used for the precipitation of
immune complexes?
A Nephelometry.
B Polyethylene glycol.
C Ammonium sulfate.
D Antibody to immunoglobulin.
E Staphylococci.
31 Antigen-specific B-cells can be purified by:
A Sedimentation rate.
B Panning on anti-Ig plates.
C Phagocytosis.
D Forward light scatter in the fluorescence activated cell
sorter (FACS).
E Binding of fluorescent antigen and separation in the FACS.
32 Antigens in tissues can be localized with fluorescent
antibodies using:
-
A Flow cytofluorimetry.
B A confocal fluorescence microscope.
C Autoradiography.
D An enzyme substrate.
E The electron microscope.
33 A gene can be selectively disrupted by:
A Electroporation with the normal gene.
B Homologous recombination.
C Liposome transfection with the normal gene.
D Antisense RNA.
E X-Irradiation.
-
Chapter 7
1 Which one of the following is a primary lymphoid organ:
A Lymph nodes
B Spleen
C Peyer's patch
D Tonsil
E T hymus
2 The thoracic duct
A Enters the spleen.
B Directly drains the lymph nodes.
C Forms the interface between the lymph and blood.
D Transports T-cells from the bone marrow to the thymus.
E Is a part of the lamina propria.
3 When antigen reaches a lymph node in a primed animal:
A There is an increase in the output of cells in the efferent
lymphatics over the following 24 h.
B There is a decrease in the output of cells in the efferent
lymphatics over the following 24 h.
C There is an immediate output of activated blast cells.
D It is transported to the spleen.
E It is all immediately destroyed by macrophages.
4 The specialized cell type involved in the entry of lymphocytes
into lymph nodes are called:
A M-cells
B Mesangial cells
C PALS
D HEV endothelial cells
E Selectins
5 The VLA molecules are:
A Homotrimers.
B Homodimers.
C Single chain molecules.
-
D Heterodimers with a common beta chain.
E Heterodimers with a common alpha chain.
6 On entering a germinal center, the primary B-blasts grow
exponentially to form which cell type in the dark zone:
A Secondary B-blasts
B Centrocytes
C Centroblasts
D Memory B-cells
E Plasma cells
7 The tingible bodies inside germinal center macrophages
are:
A DNA fragments.
B Phagocytosed foreign antigen.
C A sign of macrophage apoptosis.
D Bacterial cell wall components resistant to degradation.
E VLA molecules.
8 The paracortical area of a lymph node comprises mainly:
A Follicular dendritic cells
B Plasma cells
C Macrophages
D B-cells
E T-cells
9 In a lymph node, the antigen pneumococcus polysaccharide SIII
leads to:
A Lymphocyte proliferation in the paracortex.
B PALS development.
C Development of cellular hypersensitivity.
D Proliferation in cortical lymphoid follicles.
E The absence of germinal centers.
10 Which of the following lymphoid tissues is
unencapsulated:
A Thymus
B Lymph node
C Spleen
-
D Tonsil
E MALT
11 Expression of a CD8 alpha-alpha homodimer is characteristic
of:
A Intraepithelial lymphocytes.
B Follicular dendritic cells.
C Eosinophils.
D Classical cytotoxic T lymphocytes.
E Classical helper T lymphocytes.
12 Which of the following functions are macrophages unable to
carry out:
A Pinocytosis.
B Phagocytosis.
C Antigen processing.
D T-cell priming.
E Antigen presentation to activated cells.
13 When Langerhans' cells differentiate into fully mature
dendritic cells (DCs) they:
A Downregulate CD1 expression.
B Increase their endocytic activity.
C Increase expression of B7.1
D Decrease expression of B7.2.
E Become resistant to infection by HIV-1.
14 Langerhans' cells are found in:
A Lymph
B Lymph nodes
C Periarteriolar lymphoid sheaths
D Skin
E Mantle zone
15 Lymphocytes:
A Enter the tissues and remain there for the rest of their
life.
B When mature are only found in secondary lymphoid organs.
C Recirculate between blood and lymphoid tissues.
D Are only educated in the thymus.
E When present in the lymph are called veiled cells.
-
16 Follicular dendritic cells:
A Can bind immune complexes.
B Are small round cells.
C Retain antigen for up to 24 h.
D Possess Fc receptors but lack C3b receptors.
E Are not found in germinal centers.
17 The spleen is largely involved with the response to antigens
which are in the:
A Tissues
B Blood
C Gut
D Lungs
E Urogenital tract
18 The germinal center is an important site of:
A Hematopoiesis.
B B-cell maturation.
C T-cell maturation.
D Myeloid cell differentiation.
E Initial germ line V gene rearrangement.
19 Lymphocytes in the lamina propria secrete large amounts
of:
A IgD.
B IgA.
C Gamma delta TCR.
D Bence Jones protein.
E Isolated secretory component.
20 The bone marrow is a site of:
A Very little antibody production.
B Antibody production against T-independent antigens only.
C Long term antibody production.
D Substantial secretion of IgD.
E Antibody production by pre-B cells.
21 An example of a privileged immunological site is the:
-
A Bone marrow
B Skin
C Testis
D Lung
E Waldeyer's ring
22 LFA-1 belongs to which family of molecules:
A Complement receptors
B Cytokine receptors
C Integrins
D VLA
E Selectins
-
Chapter 8
1 CD8 is a marker of:
A B-cells
B Helper T-cells
C Cytotoxic T-cells
D An activated macrophage
E A neutrophil precursor
2 CD4:
A Is essentially an intracellular glycoprotein.
B Is heterodimeric.
C Binds processed peptide in its outer groove.
D Binds to MHC class II on antigen-presenting cells.
E Is highly polymorphic.
3 The following is characteristic of B- but not T-cells:
A Class I MHC.
B CD3.
C Measles virus receptor.
D Polyclonal activation by concanavalin A.
E Surface immunoglobulin.
4 When a resting naive T-cell engages its specific MHC/peptide
complex displayed on the surface of a fibroblast it:
A Undergoes blast cell formation.
B Produces IL-2.
C Moves from Go to G1 of the cell cycle.
D Becomes anergic.
E Secretes IL-1.
5 The T-cell ligand binding B7 on a professional
antigen-presenting cell is:
A CD28
B CD2
C LFA-1
D ICAM-1
E VCAM-1
-
6 Proliferation of activated T-cells:
A Is stimulated by a single signal induced by engagement of the
T-cell receptor.
B Requires both the signal described in A plus costimulation
from B7.
C Requires both the 2 signals described in B plus interaction
between LFA-1 and ICAM-1.
D Requires only mutual binding of LFA-3 and CD2 on the
antigen-presenting cell and T-cell respectively.
E Is unaffected by anergy.
7 Sos (Son of sevenless):
A Itself directly activates MAP kinase kinase kinase.
B Recruits the Grb2 adaptor protein.
C Is a guanine nucleotide exchange factor.
D Is a GTPase-activating protein.
E Is phosphorylated by Mek.
8 Protein tyrosine kinase activity following T-cell
stimulation:
A Phosphorylates and thereby activates phospholipase C gamma
1.
B Is an inherent property of the T-cell receptor alpha and beta
chains.
C Is an inherent property of CD3.
D Is unaffected by herbimycin A.
E Is unrelated to phosphorylation of the CD3-associated zeta
chains.
9 The early increase in phospholipase C gamma 1 activity
following T-cell stimulation:
A Represents a sensitive regulatory negative feedback control
mechanism.
B Dephosphorylates protein tyrosine kinase inhibitors.
C Accelerates hydrolysis of diacylglycerol.
D Accelerates hydrolysis of phosphatidylinositol
diphosphate.
E Accelerates hydrolysis of inositol triphosphate.
10 The nuclear AP-1 site responsible for 90% of IL-2 enhancer
activity binds:
A The Oct 1 transcriptional factor.
B The Fos/Jun transcription factors.
C The nuclear factor of activated T-cells (NFAT).
D The NF-kappa B transcriptional factor.
E Polyclonal mitogenic agents such as concanavalin A.
-
11 In the immunological synapse:
A The acetylcholine receptor in expressed on the T-cell.
B Production of substance P by the antigen-presenting cell is a
key event.
C The initial interaction between TCR and MHC is unstable.
D Adhesion molecule pairs eventually move to the center of the
synapse.
E The B7CD28 interaction is redundant.
12 T-cell mutants lacking CD45 cannot transduce signals received
through the specific T-cell receptor because CD45:
A Has protein tyrosine kinase activity.
B Directly phosphorylates CD4.
C Is a calcium ion channel.
D Removes a phosphate group from a negative regulatory site on
the Lck kinase.
E Acts as a nuclear transcription factor.
13 Lipopolysaccharide (LPS) from Gram-negative bacteria is:
A A thymus-dependent antigen.
B A type 2 thymus-independent antigen.
C A polyclonal activator of murine B-cells.
D Cross-links Ig receptors on B-cells.
E Produces high affinity IgG memory responses.
14 The carrier T-cell epitope on a thymus-dependent antigen:
A Behaves like a hapten.
B Needs to be polymeric.
C Need not be physically connected to the B-cell epitope.
D Is a carbohydrate.
E Stimulates help for the B-cell response.
15 T-cell help for antibody production:
A Depends on T-cell recognition of native antigen bound to
B-cell surface Ig.
B Depends on T-cell recognition of antigen processed by the
B-cell.
C Involves class I MHC on the B-cell.
D Can occur in X-irradiated mice.
E Is a feature of the antibody response to pneumococcal
polysaccharide SIII.
16 Cross-linking of B-cell surface receptors:
-
A Is a characteristic feature of thymus-dependent antigens.
B Lowers the intracellular Ca++ concentration.
C Rapidly phosphorylates the Ig-alpha and Ig-beta chains of the
surface Ig receptor.
D Requires contiguity of 2 B-cell epitopes of different
specificity on the same antigen molecule.
E Cannot be achieved by anti-idiotypic antibodies.
17 Activation of resting B-cells by T-helpers depends directly
upon costimulatory interaction between:
A CD40 and CD40L.
B B7 and CD28.
C B7 and CTLA-4.
D CD4 and MHC class II.
E ICAM-1 and LFA-1
18 B-cells as distinct from T-cells:
A Are polyclonally activated by phytohemagglutinin.
B Bear surface Ig receptors for antigen.
C Bear surface CD3 molecules.
D Are lymphocytes.
E Can be activated by stimulation through the antigen receptor
alone.
19 The T-cell receptor link to MHC/peptide is enhanced by
interaction between MHC class II on the antigen-presenting cells
with the following molecule on the T-cell:
A LFA-1
B CD2
C CD4
D CD8
E CD28
20 The main costimulatory signal for activation of resting
T-cells is provided by ligation of:
A CD28
B Surface Ig
C LFA-1
D VLA-4
E IL-2
-
21 Which one of the following events occurs earliest in T-cell
signaling:
A Activation of phospholipase C.
B Activation of protein kinase C.
C Production of inositol triphosphate.
D Activation of protein tyrosine kinase.
E Mobilization of intracellular calcium.
22 On injection into mice, bovine serum albumin conjugated with
dinitrophenol (DNP) behaves as a:
A Type 1 thymus-independent antigen.
B Type 2 thymus-independent antigen.
C Thymus-dependent antigen.
D A polyclonal T-cell activator.
E Hapten.
23 T-cell CD40L provides a costimulatory signal to B-cells by
ligating:
A Surface Ig
B MHC class II
C CD28
D CD19
E CD40
-
Chapter 9
1 Which is the first of the following genes to be upregulated
subsequent to T-cell activation:
A Transferrin receptor
B Cytokine receptor
C Cytokine
D c-myc
E VLA-1
2 Cytokines always act:
A By binding to specific receptors.
B In an autocrine fashion.
C At long range.
D Antagonistically with other cytokines.
E Synergistically with other cytokines.
3 A Cytokine receptor which is a member of the hematopoietin
receptor family is:
A IL-8 receptor.
B IFN gamma receptor.
C TNF (TNF-alpha) receptor.
D IL-1 receptor.
E IL-2 receptor.
4 The alpha beta heterodimeric form of the IL-2 receptor:
A Is downregulated on activated cells.
B Binds IL-2 with high affinity.
C Is found only on T-cells.
D Uses CD45 as an alpha chain.
E Allows rapid dissociation of bound IL-2.
5 IFN-gamma and TNF (TNF alpha) can act synergistically:
A To downregulate expression of MHC class I.
B Because IFN-gamma downregulates expression of TNF
receptors.
C To upregulate expression of MHC class II.
D Because they both bind to the same receptor.
E Because they cross-link IFN-gamma and TNF beta receptors.
-
6 Which of the following is characteristically produced by the
Th2 CD4 cells which provide help for antibody production, but not
by Th1 cells?:
A IFN-gamma
B Lymphotoxin (TNF-beta)
C GM-CSF
D IL-4
E IL-1
7 Dendritic cells can be driven from a resting state to an
activated state by the T-cell surface molecule:
A TCR
B CD40L
C CD28
D B7
E CD40
8 In the germinal center, B-cells become memory cells under the
influence of:
A CD40
B CD23
C IL-1 alpha
D IL-4
E TGF beta
9 High affinity B-cell clones in mammals are usually generated
by:
A Somatic hypermutation.
B Expression of high affinity precursors in the virgin (naive)
B-cell population.
C Class switching.
D Apoptosis.
E Gene conversion.
10 Prior to class switching, B-cells express:
A IgA alone
B IgA and IgG
C IgM and IgD
D IgD alone
E No surface Ig
-
11 The cytokine which is most involved in the class switch to
IgE production is:
A IL-1
B IL-2
C TGF beta
D IL-4
E IL-5
12 The first event following the production of CH sterile
transcripts by immunoglobulin genes is:
A VDJ rearrangement.
B Light chain rearrangement.
C Somatic hypermutation.
D Apoptosis.
E Class switching.
13 The specificity of antibody secreted by a B-cell may not be
the same as that of the surface Ig of the clonal parent because
of:
A Class switching.
B Somatic hypermutation.
C Allelic exclusion.
D Alternative splicing.
E Different heavy:light pairing.
14 The major long term source of a foreign antigen in the body
is:
A Anti-idiotype.
B Complexes on the surface of follicular dendritic cells.
C Antigen bound to the surface of B-cells.
D Antigenic peptides in the groove of MHC molecules.
E There are no long term sources of foreign antigen in the
body.
15 Effector memory T-cells can be distinguished from the central
memory T-cells from which they arise by:
A Their life-span.
B CD45RA.
C CCR7.
D CD3.
E IL-4 secretion.
-
16 Cytokines:
A Are usually around 150200 kDa.
B Have glycosyl phosphatidylinositol (GPI) anchors.
C Can be pleiotropic.
D Generally act at long range.
E Produce very stable long-lived messenger RNA.
17 Th1 cells secrete:
A CD4.
B IL-4.
C IL-5.
D IL-6.
E Interferon-gamma.
18 Which one of the following cytokines can mediate release of
acute phase proteins from the liver?:
A IL-10.
B TGF beta.
C IL-6.
D IL-12.
E IL-8.
19 Which of the following is not a feature of germinal center
B-cells:
A Immunoglobulin gene class switching.
B Apoptosis.
C Initial VDJ rearrangement.
D Somatic hypermutation.
E Proliferation.
20 Low amounts of antigen:
A Produce low affinity antibodies.
B Do not influence the affinity of antibodies.
C Produce high affinity antibodies.
D Affect the avidity but not the affinity of antibodies.
E Preferentially induce class switching.
21 Memory T-cells:
-
A Are continuously produced directly from naive progenitors
without the need for antigenic stimulation.
B Express high levels of CD44.
C Are only present in primary immune responses.
D Switch from an alpha beta to a gamma delta T-cell
receptor.
E Express germ line Ig V genes.
22 Activated memory T-cells can be distinguished from naive
T-cells on the basis of expression of:
A T-cell receptor.
B Immunoglobulin.
C MHC class II molecules.
D CD45RO.
E IL-1.
-
Chapter 10
1 A major factor regulating the adaptive immune response is:
A The neutrophil.
B Complement membrane attack complex.
C C-reactive protein.
D Antigen concentration.
E Haptoglobin.
2 Antigenic competition:
A Involves competition between T-cell epitopes for the MHC
groove.
B Involves competition for available soluble antibodies.
C Is unrelated to the concept of dominant and subdominant
epitopes.
D Can only occur with cryptic epitopes.
E Refers to the differential immunogenicity of the carbohydrate
and protein moieties in a glycoprotein.
3 Negative feedback on adaptive B-cell responses is mediated
by:
A Antigen specific IgM.
B Antigen specific IgG.
C Just antigen neutralization.
D Fc gamma receptors on macrophages.
E F(ab')2 anti-mu.
4 An example of an anti-apoptotic molecule is:
A Bcl-2.
B TRAIL.
C Bax.
D FADD.
E Caspase 8.
5 Injection of a mouse with a very high dose of sheep
erythrocytes induces:
A A generalized antigen non-specific suppression.
B Antigen-specific T-suppression.
C Class switching to IgM antibody production.
D IFN-gamma production by Th2 cells.
E A high antibody response.
-
6 Suppression of Th2 by Th1 cells may be mediated by:
A IL-1.
B IL-3.
C IL-4.
D GM-CSF.
E Interferon-gamma.
7 Cells bearing MHC class I plus peptide are targets for
specific:
A B-cells.
B Cytotoxic T-cells.
C Th1 cells.
D Th2 cells.
E Interdigitating dendritic cells.
8 Natural antibodies:
A Are mostly IgG.
B Are mostly high affinity autoantibodies.
C Are produced spontaneously by CD5+ B-cells.
D Are acquired by transplacental passage from the mother.
E Do not arise in thymectomized mice.
9 Antibodies specific for different epitopes on the same
antigen:
A Will usually bind to each other through idiotypic
interactions.
B Cannot bear similar idiotypes.
C Can bear similar idiotypes independently of their antigenic
specificity.
D Can only bear similar idiotypes if the antigenic epitopes
cross-react.
E Must be of the same immunoglobulin class.
10 Regulatory idiotypes on antibodies:
A Are not usually 'public' idiotypes.
B Are not usually 'cross-reactive' idiotypes.
C Are usually common to several different antibodies.
D Are usually 'private' idiotypes .
E Are usually restricted to members of a single clone of
antibodies.
11 An internal image monoclonal anti-idiotype:
-
A Has specificity for its own internal structure.
B Is the mirror image of the idiotype.
C Has identical idiotypes on heavy and light chains .
D Mimics a single epitope on the antigen.
E Cannot function as a 'surrogate' antigen.
12 High responder 'Biozzi' mice:
A Phagocytose carbon particles faster than low responders.
B Were obtained by breeding wild mice through 5 generations,
selectively mating the high responders each time.
C Differ from low responders by a single gene .
D Produce high titer antibodies to only a very restricted number
of antigens.
E Produce high titer antibodies to a variety of antigens.
13 A high antibody response to an antigen such as (TG)-A-L in a
given mouse strain:
A Is linked to immunoglobulin gene polymorphism.
B Is independent of MHC (H-2) haplotype.
C Depends on T-cell recognition of the antigen bound to MHC
class II .
D Depends on T-cell recognition of the antigen bound to MHC
class I.
E Reflects a general propensity to make high titer antibody
responses.
14 Which of the following is never related to poor MHC-linked
immune response:
A Defective T-cell repertoire.
B T-suppression.
C Inadequate antigen processing.
D Poor binding of peptide epitope to MHC.
E Defective V gene rearrangement.
15 Relative to males, females:
A Are less resistant to T-cell tolerance.
B Are more susceptible to autoimmune disease.
C Produce lower antibody responses to T-independent
antigens.
D Have lower serum Ig levels.
E Have lower levels of secretory IgA.
16 Immune responses are:
-
A Depressed by stress.
B Stimulated by glucocorticoids.
C Depressed by estrogens.
D Stimulated by androgens.
E Depressed by growth hormone.
17 Stimulation of glucocorticoid synthesis by IL-1:
A Provides a basis for a feedback suppressor circuit.
B Cannot provide a basis for a feedback suppressor circuit.
C Augments IL-1 synthesis.
D Augments cell-mediated immunity.
E Augments IFN-gamma synthesis.
18 Protein-calorie malnutrition does not affect:
A Lymphoid tissue.
B The level of circulating CD4 T-cells.
C Cell-mediated immunity.
D The affinity of antibody responses.
E Phagocytosis of bacteria.
19 The stress induced by severe exercise can increase
susceptibility to infection by:
A Raising plasma levels of cortisol.
B Increasing lymph flow.
C Increasing synthesis of adrenaline.
D Damaging mast cells.
E Increasing the respiratory rate.
20 The level of antigen:
A Is unrelated to the intensity of the immune response.
B Is related to the intensity of the immune response.
C Never has an effect on the response to a T-independent
antigen.
D Has no effect on the concentration of MHC bound processed
antigen.
E Is constant in the extracellular fluid.
21 IgG antibodies:
A Are the earliest class to appear in an immune response.
B Are normally dimeric in mucosal secretions.
-
C Remain of low affinity.
D Inhibit antibody responses through the Fc gamma receptor
FcgRIIB1 on B-cells.
E Augment antibody responses through the Fc gamma receptor
FcgRIIB1 on B-cells.
22 T-cell mediated suppression:
A Is always mediated by CD8+ T-cells.
B Is always mediated by CD4+ T-cells.
C Can be due to Th2 cells suppressing Th1 by IL-4 or IL-10.
D Does not exist.
E Cannot transfer suppression to another animal.
23 Idiotypeantiidiotype interactions:
A Do not involve antigen-specific receptors.
B Only involve antibodies.
C Can mediate a lymphocyte network in early life.
D Do not occur within the CD5+ B-cell population.
E Do not involve self-reactivity.
24 High antibody responses linked to MHC class II genes:
A Are independent of MHC haplotype.
B Depend on good T-helper cell recognition of class II/antigen
peptide complex.
C Depend on defective antigen presentation by MHC molecules.
D Result from tolerance to MHC/self-peptide complexes.
E Require suppression of Th2 cells.
25 With respect to immunoneuroendocrine networks:
A They do not exist.
B They exist only in higher primates.
C They never involve ACTH.
D They never involve the hypothalamic/pituitary axis.
E Augmentation of glucocorticoid production by IL-1 is a good
example.
26 Cell-mediated immunity:
A Is unaffected by exercise.
B Is unaffected by surgical trauma.
C Is unaffected by diet.
-
D May be grossly impaired by protein-calorie malnutrition.
E Is unaffected by protein-calorie malnutrition.
-
Chapter 11
1 Which of the following is the earliest site of hematopoiesis
in the embryo:
A Bone marrow
B Liver
C Spleen
D Yolk sac
E Thymus
2 Stem cell factor is produced mainly by;
A Stem cells.
B Thymic epithelium.
C Bone marrow stromal cells.
D Megakaryocytes.
E The reticuloendothelial system.
3 The putative murine stem cell is positive for:
A B220
B Mac-1
C Gr-1
D CD8
E Sca-1
4 An example of a peptide hormone produced by the thymus is:
A Thymulin
B Thyroglobulin
C Thyroxine
D Thy-1
E Thymine
5 Hassall's corpuscles are found in:
A Peripheral blood
B Bone marrow
C Spleen
D Thymus
E Peyer's patches
-
6 Which of the following statements might not be true?:
A Pluripotent hematopoietic stem cells are produced in the bone
marrow.
B In adults, the thymus is virtually atrophied and is therefore
no longer functional.
C Thymocytes are educated in the thymus.
D Not all lymphocytes are leukocytes.
E Plasma cells are always derived from B-lymphocytes.
7 In a thymectomized animal which is irradiated and
reconstituted with bone marrow cells:
A The T-lymphocyte population is not restored.
B The B-lymphocyte population is not restored.
C All lymphocyte populations are restored.
D None of the lymphocyte populations are restored.
E Rapid death inevitably follows.
8 In the thymic medulla the majority of gamma delta T-cells
are:
A CD4+CD8+
B CD4+CD8
C CD4CD8+
D CD4CD8
E Surface Ig+
9 NKT cells:
A Have rearranged TCR alpha beta genes.
B Express a gamma delta TCR.
C Have rearranged immunoglobulin genes.
D Lack NK1.1.
E Can only recognize antigens presented by CD1.
10 CD8+ T-cells bearing an alpha beta TCR:
A Always co-express CD4.
B Will have expressed terminal deoxynucleotidyl transferase
(TdT) during their early development.
C Do not require the presence of a thymus for their
development.
D Recognise native antigen.
E Recognise MHC class II molecules.
11 Alpha beta T-cells:
-
A Initially express a pre-TCR bearing an invariant pre-beta
chain.
B Arise earlier in ontogeny than gamma delta T-cells.
C Can be CD4+CD8 but not CD4CD8+.
D Unlike gamma delta T-cells, do not rely on Notch-1 signaling
pathways during their development.
E Express RAG-1 and RAG-2 at the pre-T stage.
12 Allelic exclusion is not seen for:
A T-cell receptor alpha genes.
B T-cell receptor beta genes.
C T-cell receptor gamma genes.
D T-cell receptor delta genes.
E T-cell receptor gene D region segments.
13 The first wave of fetal gamma delta T-cell receptor cells in
mouse colonize the:
A Female reproductive tract.
B Skin.
C Peripheral blood.
D Thymus.
E Gut.
14 In human adult peripheral blood the percentage of gamma delta
T-cells bearing V gamma 9, V delta 2 T-cell receptors is:
A < 1%
B 25%
C 50%
D < 30%
E 70%
15 The T-cells in male H-2b SCID mice bearing a transgenic alpha
beta T-cell receptor gene specific for HY in association with H-2Db
will:
A All be CD4+CD8.
B All be CD4CD8+.
C All recognise H-Y alone.
D Undergo continuous proliferation.
E Be deleted.
-
16 In Sir Peter Medawar's classical experiments demonstrating
the development of immunological tolerance:
A Thymectomized mice had to be used.
B SCID mice were used.
C The mice were chimeric.
D Skin grafts were always rejected.
E Tolerance could not be induced in neonatal mice.
17 In infectious anergy:
A T-cells become deleted.
B Anergic T-cells indirectly suppress other T-cells.
C CD80 on the antigen-presenting cell becomes upregulated.
D MHC class II on the antigen-presenting cell becomes
upregulated.
E T-cells recognize antigen in the absence of antigen-presenting
cells.
18 Differentiation of B-cells from hematopoietic stem cells is
independent of:
A Ikaros
B IL-3
C IL-7
D Pax5
E IL-8
19 The phenomenon whereby, following successful Ig gene
rearrangement, further rearrangement on the sister chromatid is
suppressed is called:
A Allelic exclusion.
B Class switching.
C Productive rearrangement.
D Clonal selection.
E Gene shuffling.
20 Strains of mice which are congenitally deficient in C5:
A Do not possess C5-responsive lymphocytes.
B Have both T-cells and B-cells that can respond to C5.
C Have T-cells, but not B-cells, that can respond to C5.
D Have B-cells, but not T-cells, that can respond to C5.
E Have C5-specific monocytes, but not lymphocytes.
21 Genuine adaptive T- and B-cell responses are seen in:
-
A The Californian hagfish.
B The horseshoe crab.
C Xenopus.
D Drosophila.
E Marine sponges.
22 A member of the immunoglobulin gene superfamily which
contains 5 Ig-type domains is:
A Thy-1
B Poly-Ig receptor
C MHC class I heavy chain
D CD4
E CD3 epsilon
23 The first antigen receptor-bearing cells to appear in the
thymus in mouse are:
A T-cell receptor alpha beta+
B T-cell receptor gamma delta+.
C TdT.
D RAG-1.
E CD3.
24 Positive selection in the thymus is mediated by:
A Thymic epithelial cells.
B Macrophages.
C Dendritic cells.
D B-cells.
E T-cells.
25 B-1a cells are:
A B-cells which respond to mostly to T-dependent antigens.
B B-cells which predominate in later life.
C Plasma cells.
D CD5 B-cells.
E CD5+ B-cells.
26 V preB:
-
A Refers to all the germ line immunoglobulin heavy chain V genes
in the pre-B cell.
B Is part of the surrogate light chain.
C Forms a structure with lambda 3.
D Forms a structure with C kappa.
E Is expressed by both T- and B-cells.
27 Which immunoglobulin class crosses the placenta to provide a
high level of passive immunity at birth?:
A IgA
B IgD
C IgE
D IgG
E IgM
28 Which of the following molecules does not belong to the Ig
gene superfamily:
A Immunoglobulin
B T-cell receptor
C Beta2-microglobulin
D Thy-1
E LFA-1
-
Chapter 12
1 The largest number of deaths from infectious diseases are
caused by:
A AIDS.
B Acute respiratory infections.
C Diarrheal diseases.
D Tuberculosis.
E Measles.
2 Histamine:
A Has no effect on the permeability of venules.
B Constricts arterioles.
C Upregulates adhesion molecules on vascular endothelium.
D Upregulates IL-8.
E Induces neutrophil chemotaxis.
3 P-selectin pairs with:
A LFA-3.
B LFA-1.
C ICAM-1.
D Sialyl Lewisx.
E Beta2 integrin molecules.
4 Binding of platelet activating factor (PAF) to its receptor on
the neutrophil:
A Initiates binding of neutrophils to P-selectin.
B Initiates neutrophil rolling.
C Produces a chemotactic gradient.
D Causes histamine release.
E Upregulates LFA-1.
5 Activated Hageman factor:
A Produces a fibrin clot in damaged veins.
B Is factor X of the intrinsic clotting system.
C Directly causes thrombus formation.
D Directly increases vascular permeability.
E Directly dissolves fibrin clots.
-
6 Neutrophil chemotaxis is mediated by:
A C5b
B IL-8
C C3a
D CCL2
E E-selectin
7 Opsonization of bacteria occurs through coating bacteria just
with:
A C3b
B C8
C Membrane attack complex
D F(ab')2 IgG
E IgM
8 Extracellular bacteria are optimally killed by:
A Macrophages.
B Complement.
C Antibody.
D Macrophages plus complement.
E Macrophages plus antibody plus complement.
9 Extracellular bacteria try to avoid killing by:
A Activating neutrophils.
B Accelerating complement activation.
C Synthesizing capsules.
D By deviating complement deposition to the cell membrane.
E Limiting variation in their antigens.
10 Toxins are neutralized by:
A Complement
B Antibody
C Toxoids
D PGE2
E Proteolytic enzymes
11 CR1 complement receptors on phagocytic cells bind:
-
A Factor H
B Factor I
C C3d
D Only inactive C3b (iC3b)
E C3b
12 Secretory IgA protects external mucosal surfaces by:
A Triggering mast cells.
B Recruiting phagocytic cells.
C Preventing microbial adherence to the mucosa.
D Binding to epithelial cells.
E Its secretory piece.
13 The pyrogenic streptococcal exotoxins SPE, A, B, and C:
A Are M proteins.
B Cause post-streptococcal autoimmune disease.
C Produce high titers of anti-streptolysin O.
D Have hyaluronidase activity.
E Are superantigens.
14 Individuals lacking C8 or C9 are more prone to infection with
the following type of bacteria:
A Haemophilus influenzae.
B Bacillus anthrax.
C Vibrio cholerae.
D Neisseria.
E Listeria monocytogenes.
15 Specific immunity to M. tuberculosis in mice can be
transferred to naive histocompatible recipients by:
A B-cells.
B T-cells.
C Macrophages.
D Neutrophils.
E IgG.
16 Lepromatous leprosy is characterized by:
-
A Poor T-cell responses.
B Good cell-mediated immunity.
C Good lepromin dermal response.
D Poor B-cell responses.
E Poor phagocytic ability..
17 Viral antigen shift involves:
A Single point mutation.
B Random point mutations.
C Interchange of large segments of the viral genome with other
viruses.
D Rearrangement of the viral structural architecture.
E Regression to an earlier variant.
18 The HIV nef protein affects antigen presentation by:
A Causing MHC class I endocytosis.
B Containing glycine-alanine repeats which inhibit
proteasome-mediated processing of the virus.
C Preventing peptide binding to TAP.
D Preventing peptide transport through the TAP pore.
E Competing for peptide binding to the MHC class I heavy
chain.
19 Interferons:
A Opsonize viruses.
B Neutralize viruses.
C Play a major role in recovery from viral infections.
D Prevent viral entry into cells.
E Protect against reinfection with virus.
20 Cytotoxic T-cells:
A Are usually CD4.
B Recognize native viral antigen.
C Are more readily produced by killed rather than live
virus.
D Kill viruses directly.
E Restrict viral replication.
21 Classical NK cells:
-
A Have rearranged T-cell receptor genes.
B Are unaffected by IFN alpha.
C Are more active against targets with upregulated MHC class
I.
D Can kill certain virally-infected cells.
E Can kill all virally-infected cells.
22 IgE levels are high in infections with:
A Trichinella spiralis.
B Trypanosoma cruzi.
C Trypanosoma rhodesiense.
D Plasmodium falciparum.
E Leishmania tropica.
23 Th1 cells are of major importance in defense against:
A Trypanosoma brucei.
B Leishmania donovani.
C Malarial merozoites.
D Malarial sporozoites.
E Trichinella spiralis.
24 Trypanosomes do not evade the host immune response by:
A Pre-empting complement defenses.
B Releasing decoy proteins.
C Varying their surface antigens.
D Escaping from the phagosome.
E Completely resisting phagocytosis.
25 Abnormally folded prion proteins are:
A Relatively sensitive to protease digestion.
B Associated with CGD.
C Present in follicular dendritic cells in individuals with
transmissible spongiform encephalopathies.
D Normally eliminated by B-cells.
E Destroyed by macrophages.
26 Immune complexinduced nephrotic syndrome is a feature of:
-
A Trypanosomiasis.
B Quartan malaria.
C Schistosomiasis.
D Leishmaniasis.
E Chagas' disease.
27 Inflammation is a defensive reaction initiated by infection
or tissue injury which firstcauses:
A Upregulation of adhesion molecules on endothelial cells and
leukocytes.
B Chemotaxis.
C IL-8 release.
D Phagocytosis.
E Bacterial killing.
28 Extracellular bacteria are killed by:
A Secreting exotoxins.
B Impeding inflammatory reactions.
C Phagocytosis and complement.
D Toxin neutralization.
E C-reactive protein.
29 The mucosal surfaces of the body are initially protected by
free:
A IgG.
B IgM.
C IgE.
D Secretory IgA.
E IgD.
30 Bacteria growing within macrophages are killed by:
A Complement.
B Reactive oxygen and nitrogen intermediates.
C Antibody.
D Cytotoxic T-cells.
E Directly by cytokines released from Th1 cells.
31 Some viruses escape the immune system by antigen shift. This
involves:
-
A Structural reorganization of existing antigens.
B Masking of antigen epitopes.
C Point mutations in single genes.
D Point mutations in many genes.
E Interchange of genetic material with other viruses.
32 Protection against worm infestations is particularly
associated with an increase in:
A IgD.
B IgE.
C IgG.
D IgA.
E IgM.
-
Chapter 13
1 The first production of live but non-virulent forms of chicken
cholera bacillus was achieved by:
A Pasteur.
B Salk
C Jenner
D Montague
E Sabin
2 The term variolation refers to:
A The generation of antibody variable regions.
B The attenuation of virulent organisms
C Innoculation of scab material into small skin wounds
D The removal of scab material from an individual with
smallpox
E A type of gene therapy
3 The circulation of a two month old breast-fed baby will
contain maternal:
A IgA
B IgD
C IgE
D IgG
E IgM
4 What is the minimum percentage of children which needs to be
vaccinated successfully in order to achieve herd immunity to
diphtheria;
A 100%
B 10%
C 50%
D 75%
E Herd immunity cannot be achieved against diphtheria
5 For vaccination against mycobacterial diseases such as
tuberculosis, the most important facet of the immune response to be
stimulated is:
A A high titer of antibody
B Macrophage-activating cell-mediated immunity
C Cytotoxic T-cells
-
D Antibody in the gut lumen
E Neutrophils
6 Which one of the following diseases has been completely
eradicated world-wide?:
A Measles
B Smallpox
C Tuberculosis
D Cowpox
E Psittacosis
7 BCG is used to protect against:
A Tuberculosis
B Rabies
C Hepatitis B
D Influenza
E Pertussis
8 A small protein subunit used in a vaccine may fail to
stimulate T-cell immunity because of:
A Lack of glycosylation
B Lack of conformation
C Lack of carrier determinants
D HLA-related unresponsiveness
E Inherently insufficient antigen concentration
9 Baculovirus vectors can be produced in cell lines derived from
which of the following:
A Moth
B Chicken bursa
C Chinese hamster ovary
D Tobacco
E Green monkey kidney
10 DNA vaccines:
A Are relatively poor at stimulating cytotoxic T lymphocyte
responses in mice
B Must be administered on gold particles if they are to be
effective
C Are only effective if followed by a protein boost
D May have distinct advantages when preparing subunit vaccines
against viruses
-
which frequently alter their antigens
E Require cold storage in tropical countries
11 A peptide immunogen:
A Adopts a single rigid structure in solution
B Can mimic a part of a discontinuous epitope
C Can elicit potent antibody responses in the absence of T-cell
help
D Can be used to stimulate B-cell but not T-cell responses
E Lack contact residues capable of interacting with the antigen
receptor on the relevant lymphocytes
12 Tetanus toxoid is usually given to humans:
A Absorbed to aluminum hydroxide
B With complete Freund's adjuvant
C Without the addition of any other agent
D Together with the toxin
E Only as a therapeutic agent, not prophylactically
13 A potential disadvantage of immunological protection using
passive transfer of horse globulins is:
A Serum sickness
B Irreversible protection
C Lack of antibody-mediated immune response
D Type IV hypersensitivity reactions
E Immunodeficiency
14 A feature of a Salmonella-based vaccine expressing antigens
from other infectious agents is that:
A Immunity is limited to the gut
B Only secretory IgA is elicited
C It does not invade the mucosal lining of the gut.
D It provokes both oral and systemic immunity.
E The organism does not need to be attenuated
15 To which one of the following groups would it be acceptable
to give a live attenuated viral vaccine?:
A Children under 8 years of age
B Patients treated with steroids
C Pregnant mothers
-
D Patients with leukemia
E Patients treated with radiotherapy
16 Idiotypes can be exploited as epitope-specific vaccines if
they are:
A IgG but not IgM
B At least bivalent
C First deglycosylated.
D Only if they are anti-idiotypes
E Internal image
17 An antibody response to a protein vaccine can only be
obtained:
A If the molecule is first linked to a carrier
B If the molecule maintains discontinuous epitopes
C If the molecule is glycosylated
D If disulfide bonds are maintained
E If the peptide bonds are maintaine
-
Chapter 14
1 Defects in neutrophil NADPH oxidase system produce:
A Chronic granulomatous disease.
B Chediak-Higashi disease.
C Leukocyte adhesion deficiency.
D Hashimoto's disease.
E Streptococcal infection.
2 Mendelian susceptibility to mycobacterial infection does not
involve the gene for:
A IFNgR1.
B IL-12 p40.
C IFNgR2.
D MEFV.
E IL-12RbI.
3 Paroxysmal nocturnal hemoglobulinuria results from deficiency
in:
A Myeloperoxidase.
B Decay accelerating factor. (DAF).
C Classical pathway C components.
D C1 inhibitor.
E C8.
4 X-linked agammaglobulinemia results from a mutation in:
A IFNg receptor.
B The CIITA promoter protein.
C An HLA gene.
D CD40L (CD154).
E A tyrosine kinase gene.
5 Di George syndrome results from a defect in:
A Purine nucleoside phosphorylase.
B WASP.
C Thymic development.
D DNA repair.
E CD3.
-
6 Mutations in the gammac chain of the receptors for IL-2, 4, 7,
9 and 15 lead to:
A Reticular dysgenesis.
B Bare lymphocyte syndrome.
C HyperIgM syndrome.
D Severe combined immunodeficiency (SCID).
E Build-up of toxic nucleotide metabolites.
7 The approximate percentage of cases of severe combined
immunodeficiency (SCID) due to defects in the Armetis gene is:
A 5%.
B 10%.
C 15%.
D 40%.
E 60%.
8 Poor skin tests to a range of microbial antigens such as
tuberculin and mumps indicate a deficiency of:
A NK cells.
B T-cells.
C B-cells.
D Phagocytosis.
E Opsonization.
9 HIV binds to:
A CD4.
B IL-2 receptor.
C NF kappa B.
D Reverse transcriptase.
E TNF receptors.
10 A coreceptor for HIV is:
A RANTES
B CD8
C CD54
D CR5
E CXCR4
11 Which of the following is not helpful in the diagnosis of
AIDS:
-
A CD4 numbers.
B CD8 numbers.
C Skin tests to bacterial antigens.
D Lymph node biopsy.
E Serum p24 antigen.
12 Which of the following HIV antigens provides a potential
target for neutralizing antibody:
A U3
B gp120
C Reverse transcriptase
D Protease
E tat
13 Primary immunodeficiency producing susceptibility to
infection by viruses and molds is due to:
A B-cell deficiency.
B T-cell deficiency.
C Phagocyte deficiency.
D Complement deficiency.
E Eosinophil deficiency.
14 Deletions in the T-cell CD154 (CD40L) gene produce:
A The hyperIgM syndrome.
B Congenital X-linked agammaglobulinemia.
C IgA deficiency.
D WiskottAldrich Syndrome.
E Deficiency in cytotoxic T-cell activity.
15 Which of the following have not provided examples of
secondary immunodeficiency:
A Viral infection.
B Lymphoproliferative disorders.
C Cytotoxic drugs.
D High fat diet.
E Low iron diet.
-
Chapter 15
1 The b chain of the FceRI:
A Binds IgE.
B Is associated with Lyn.
C Contains a GPI-anchor.
D Possesses an ITIM sequence.
E Is also used by FceRII.
2 Which one of the following mast cell products is not preformed
and therefore has to be newly synthesized?:
A Histamine.
B Prostaglandin D2.
C Heparin.
D Neutral protease.
E Eosinophil chemotactic factor (ECF).
3 Lol p1-11V are allergens cloned from:
A Rye grass pollen.
B House dust mite.
C House dust mite feces.
D Animal danders.
E Dermatophagoides pteryonyssinus.
4 Type I hypersensitivity can be blocked using:
A Histamine.
B An IgA myeloma.
C A myeloma protein of mixed antibody class.
D Sodium cromoglycate.
E Interleukin-5.
5 A major unresolved question concerning ADCC is:
A Whether antibody is involved.
B Whether it can be carried out by NK cells.
C Whether it leads to cell death.
D Whether it is complement-dependent.
E To what extent it occurs in vivo.
-
6 Mycoplasma pneumoniae infection can lead to:
A Hashimoto's thyroiditis.
B Agranulocytosis.
C Cold hemagglutinin disease.
D Thrombocytopenic purpura.
E Neonatal alloimmune thrombocytopenia.
7 The term reactive lysis usually refers to a sequence of events
involving:
A Natural killer (NK) cells.
B Cytotoxic T-lymphocytes (CTL).
C Antibody-dependent cellular cytotoxicity (ADCC).
D Th1 cells.
E Complement.
8 Maple bark stripper's disease is a hypersensitivity largely
affecting the:
A Skin.
B Kidneys.
C Nervous system.
D Lung.
E Platelets.
9 Dead Wuchereria bancrofti can cause:
A Elephantiasis.
B Erythema nodosum leprosum.
C Serum sickness.
D Pigeon fancier's disease.
E Farmer's lung.
10 Chronic granuloma represents an attempt by the body to:
A Wall off a site of chronic infection.
B Make a site of chronic infection accessible.
C Digest antibody-antigen complexes.
D Initiate an immune response.
E Change from a Th1 to a Th2 type of response.
11 In thyroid autoimmunity, an antibody causing type V
hypersensitivity may be present and is directed against:
-
A Thyroglobulin.
B Thyroid peroxidase.
C Thyroid stimulating hormone (TSH) receptor.
D Acetylcholine receptor.
E Thyroxine.
12 Which type of hypersensitivity cannot be transferred with
serum antibody?:
A Type I.
B Type II.
C Type III.
D Type IV.
E Type V.
13 Anaphylaxis can be triggerred by cross-linking of IgE
receptors on:
A Monocytes.
B Mast cells.
C B-cells.
D Eosinophils.
E Neutrophils.
14 Rhesus hemolytic disease of the newborn involves:
A IgE.
B Antibody to cell surfaces.
C Soluble immune complexes.
D Cytokine release from T-cells.
E Stimulatory antibodies.
15 The Arthus reaction is characterized by an intense
infiltration by:
A Mast cells.
B Neutrophils.
C Eosinophils.
D Macrophages.
E Langerhans' cells.
16 Type IV hypersentivity is often referred to as:
A Immediate.
