ORAL AND MAXILLOFACIAL PATHOLOGY OOOO

e212 Abstracts February 2014

PE-410 - IMMUNOHISTOCHEMICAL EXPRESSIONOF CYTOKERATINS IN CENTRAL AND PERIPHERALODONTOGENIC FIBROMAS. BRUNNO SANTOS DEFREITAS SILVA, KARIN SÁ FERNANDES, MARIA FÁTIMAGUARIZO KLINGBEIL, FERNANDA PAULA YAMAMOTO-SILVA, DÉCIO DOS SANTOS PINTO-JÚNIOR. CURSO DEODONTOLOGIA DO CENTRO UNIVERSITÁRIO DEANÁPOLIS/GO.

This study investigated the immunohistochemical expres-sion of cytokeratins (CK) 5/6, 7, 8, 10, 13, 14, 18, and 19 in fivecases of central odontogenic fibromas (COF), nine peripheralodontogenic fibromas (POF), and three dental follicle speci-mens. Study Design: Paraffin-embedded sections were obtainedfor the immunohistochemical evaluation of CK monoclonalantibodies. Results: CK 14 expression was positive in theepithelial islands of all cases. In four OF cases (28.57%) a weakpositivity to CK 19 was observed in some central stellate cells(three POF and one COF). Of these four cases, three were WHOtype and one simple type. Strong expression of CK 7 wasdetected in some central stellate cells and peripheral columnarcells of three cases of POF (21.43%). Conclusion: CK expres-sion in OFs suggested a variable degree of epithelium matura-tion, demonstrating a possible difference in COF and POForigin.

PE-411 - IMMUNOHISTOCHEMICAL EXPRESSION OFLANGERHANS CELLS IN ACTINIC CHEILITIS ANDLOWER LIP SQUAMOUS CELL CARCINO-MA. MARIANNE DE VASCONCELOS CARVALHO,FELIPE PAIVA FONSECA, ROGÉRIO GONDAK,JULIANE OLIVEIRA GOMES, MÁRCIO AJUDARTELOPES, PABLO AGUSTIN VARGAS. FOP/UNICAMP.

Oral mucosal Langerhans cells (LCs) are involved inimmunosurveillance against neoantigens associated with malig-nant transformation. This study compared the presence and dis-tribution of LCs in actinic cheilitis (AC) with/without dysplasiaand correlated findings with those in lower lip squamous cellcarcinoma (LLSCC). Study Design: Forty-eight cases of AC and21 of LLSCC were retrieved from the archives of the Departmentof Oral Diagnosis (Pathology) of the State University of Cam-pinas (Piracicaba Dental School, Brazil). Immunohistochemicalanalysis was performed with antibodies anti-CD83 and anti-CD1a. The quantification of LCs was performed and the resultsexpressed as positive cells per area of epithelium. Results:Increased grade of dysplasia was accompanied by a decrease inthe population of LCs and a depletion of these cells in LLSCCsamples (p < 0.001). Conclusion: Depletion of LC in lipepithelium may represent a higher risk for malignant trans-formation of oral lesions such as ACs.

PE-412 - IMMUNOHISTOCHEMICAL PROFILE OFORAL BENIGN PERIPHERAL NERVE SHEATH TUMORS.GARDÊNIA MATOS PARAGUASSÚ, LUANA COSTABASTOS, LEONARDO PROVEDEL, CLARISSA ARAÚJOSILVA GURGEL, LUCIANA MARIA PEDREIRA RAMALHO,JEAN NUNES DOS SANTOS, FLÁVIA CALÓ AQUINOXAVIER. UNIVERSIDADE FEDERAL DA BAHIA.

Schwannoma and neurofibroma are rare benign peripheralnerve sheath tumors that can be difficult to diagnose based onhistopathological analysis. Immunohistochemical analysis may

facilitate diagnosis. This study evaluated the histopathologicaland immunohistochemical features of oral schwannomas andneurofibromas diagnosed in an oral pathology service between2002 and 2012. Two schwannomas and three neurofibromas wereselected and reviewed from a total of 2,114 oral lesion cases.Immunohistochemical tests included anti-S100, anti-vimentin,anti-VEGF, anti-CD-34, and anti-tryptase antibodies. All tumorsdemonstrated positive immunostaining for S-100 and showedvariable immunostaining intensity for vimentin and VEGF intumors. CD-34 immunostaining revealed more blood vesselsin schwannomas (13.2 � 0.57) compared to neurofibromas (12.54� 0.5). Tryptase immunostaining revealed more mast cells inneurofibromas (5.6 � 5.44) than in schwannoma (3 � 4.24).Therefore vascular endothelial factors and mast cells maycontribute to the diagnosis of these tumors and have implicationsfor their pathogenesis.

PE-413 - IMMUNOHISTOCHEMICAL STUDY OFCYCLIN D1, COX-2, AND PCNA IN PLEOMORPHICADENOMAS. JEFFERSON DA ROCHA TENÓRIO,LEORIK PEREIRA DA SILVA, GEORGE JOÃO FERREIRADO NASCIMENTO, GLEICY FÁTIMA MEDEIROS DESOUZA, ANA PAULA VERAS SOBRAL. FACULDADE DEODONTOLOGIA DE PERNAMBUCO - UNIVERSIDADEDE PERNAMBUCO.

Several studies have associated cell cycle proteins andcyclooxygenase-2 (Cox-2) with an increase of neoplastic cellproliferation levels. This work evaluated immunohistochemi-cal expression of cyclin D1 (CD1), Cox-2, and proliferatingcell nuclear antigen (PCNA) in pleomorphic adenomas(PAs). Materials and Methods: Clinicopathological dataand 3-mm-thick sections of 48 PAs were retrieved from filesof the Laboratory of Oral Pathology, Dental School, Univer-sity of Pernambuco, Camaragibe, Brazil. Anti-CD1, -Cox-2and -PCNA monoclonal antibodies were used in immunohisto-chemical staining. Results: Immunoexpression of CD1, PCNA,and Cox2 exhibited positivity in, respectively, 57.9%, 54.2%and 43.8% of PAs (p > 0.05). No statistical associations werefound between the proteins studied and the clinicopathologicaldata. Results appear to show that cell proliferation is character-istic of the neoplastic behavior of PAs. Conclusion: Cell cycleand proliferation regulatory proteins (CD1 and Cox-2) areinvolved in the neoplastic growth of PAs independently of theirclinicopathological features.

PE-414 - IMPACT OF PAIN ON QUALITY OF LIFE INHEAD AND NECK CANCER. SANDRA LÚCIA VENTORINVON ZEIDLER, MARIANE GARCIA OLIVEIRA,MARCELLA SOL, KARINE GADIOLI OLIVEIRA, JOSEROBERTO VASCONCELOS PODESTÁ, NAZARÉ SOUZABISSOLI, SONIA ALVES GOUVEA. FEDERAL UNIVER-SITY OF ESPÍRITO SANTO, VITORIA, ES.

This study evaluated the quality of life (QoL) and painlevels in patients with head and neck squamous cell carcinoma(HNSCC). Materials and Methods: Interviews were conductedof 127 patients with HNSCC. Pain severity was measured usingthe Brief Pain Inventory (BPI) questionnaire, and QoL wasassessed with the EORTC QLQ-C30 and the QLQ-H&N35module. Data were analyzed using SPSS software and the Chi-square test (p < 0.05). Results: Mean patient age was 57.9years, and there was a predominance of males. Most cases