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IGF-1 GENE IGF-1 GENE EXPRESSION: an EXPRESSION: an
instantaneous growth instantaneous growth indicator for tilapiaindicator for tilapia
Emmanuel Vera Cruz, Emmanuel Vera Cruz, Christopher Brown, Christopher Brown, Remedios Bolivar, Russell Remedios Bolivar, Russell Borski, students, and Borski, students, and colleaguescolleagues
Cloning, diet (2006) Cloning, diet (2006) Aquaculture 251:585-595. Aquaculture 251:585-595.
Social Status (2007) Hormones Social Status (2007) Hormones and Behavior. 51:611-619.and Behavior. 51:611-619.
Temperature dependence, in Temperature dependence, in presspress
Influence of photoperiod, in press Influence of photoperiod, in press
Isolation of IGF-1 Isolation of IGF-1 mRNA, mRNA, Oreochromis Oreochromis
niloticusniloticus 1 TTCTCCAAAACGAGCCTGCGCAATGGAACAAAGTCGGAATATTGAGATGTGACATCGCC 59 60 CGCATCTCATCCTCTTTCTCCCTGTTTTAATGACTTTAAACAAGTTCATTTTCGTCGGGC 119 Signal Peptide (144-275) 120 TTTGTCTTGTGGAGACCCGTGGGGATGTCTAGCGCTTTTTCCCTTCAGTGGCATTTATGT 179 M S S A F S L Q W H L C 180 GATGTCTTCAAGAGTGCGATGTGCTGTATCTCCTGTAGCCACACCCTCTCACTACTGCCG 239 D V F K S A M C C I S C S H T L S L L P B domain(276-362) 240 TGCGTCCTCACCCTGACTCCGACGGCAACAGGGGCGGGCCCTGAGACCCTGTGCGGGGCG
299 C V L T L T P T A T G A G P E T L C G A 300 GAGCTGGTCGACACGCTGCAGTTTGTCTGTGGAGAGCGAGGCTTTTATTTCAATAAACCA 359 E L V D T L Q F V C G E R G F Y F N K P C domain (363-392) A domain (393-455) 360 ACAGGCTATGGCCCCAGTGCAAGGCGGTCACGTGGCATCGTGGACGAGTGCTGCTTCCAA 419 T G Y G P S A R R S R G I V D E C C F Q D domain (456-479) 420 AGCTGTGAGCTGCAGCGCCTTGAGATGTACTGTGCACCTGTCAAGACTCCCAAGATTTCT 479 S C E L Q R L E M Y C A P V K T P K I S E domain (480-539) 480 CGCTCTGTGCGTTCACAGCGGCACACAGACATGCCAAGAGCACCCAAGGTTAGTAGCAGA 539
Close (84-90%) sequence identity with other IGF-1s
Hepatic gene Hepatic gene expression as related expression as related
to weight gainto weight gain
Temperature-Temperature-dependent differences dependent differences in weight gain and IGF-in weight gain and IGF-
1 expression1 expression
Effect of environmental Effect of environmental temperature on IGF-1 temperature on IGF-1
expressionexpression
Social dominance Social dominance and IGF-1 expressionand IGF-1 expression
Correlation of Correlation of aggression w/ IGF-1 aggression w/ IGF-1
expressionexpression
Photoperiod-induced Photoperiod-induced changes, growth & IGF-changes, growth & IGF-
11
Temperature Temperature influence on growth influence on growth
raterate
Correlative change Correlative change in IGF-1 expression…in IGF-1 expression…
… … resulting from tight resulting from tight association of growth association of growth with IGF-1 expressionwith IGF-1 expression
AcknowledgementsAcknowledgements Funding agenciesFunding agencies
USAID/PDA CRSPUSAID/PDA CRSP The Fulbright FoundationThe Fulbright Foundation
InstitutionsInstitutions Central Luzon State Central Luzon State
UniversityUniversity North Carolina State North Carolina State
UniversityUniversity Florida International Florida International
UniversityUniversity NOAA, The Milford NOAA, The Milford
LaboratoryLaboratory
Thank you!Thank you!