If = Hypothesis, Then = PredictionPrediction= What you think the outcome of a specific experiment will be

Hypothesis: Why

If /then statement is NOT A HYPOTHESISYou will be marked wrong.

You Should Know

• Scientific Method• Science Terms– Control– Controlled Variable,– Indpendent/dependent variables– Sample size– Significance– Etc….

• Tools we have learned in lab. From onions- transformations. Big picture.

• Hint: We are testing Scientific reasoning and problem soloving.

Transformations Terms

– Biotechnology: using technology to manipulate organisms

– Genetic Engineering: Manipulating genes in organisms

– DNA Cloning: putting a segment of DNA in an organism and having that organism make tons of copies of the gene, gene product or DNA segment.

– Transformation: Introducing DNA from one organism (usually bacteria) into another organism.

Very Expensive

2 stops

Will be Graded on Technique

Be patient

Restriction Enzymes Leave Sticky Ends

Transformation

http://www.youtube.com/watch?v=aA5fyWJh5S0

How do we know it worked?

• Must determine if Gene was Inserted Successfully into Plasmid– Marker Genes!– GFP– Disrupt function and it disappears

• Antibiotic: tells if plasmid made it in to cell• Color/Glowing: tells if gene made it into

plasmid

Problem

Plates

• Groups of 3 or 4. • Half Plates: step 7 page 104 use half volumes– 50 instead of 100, 25 instead of 50

• Check Plates 48 hours later mark on plate cover what you found. – Leave in incubator for me to grade.