Thermo Scientific HyPerforma S.U.B. TK (Jacketed)Quick Start Guide

Thermo Scientific HyPerforma S.U.B. TK (Jacketed)Quick Start GuideRev. 1.0

Thermo Scientific 3 S.U.B. TK Jacketed Quick Start Guide

Quick Start Guide

Section 1 TCU Setup and Installation

NOTES:

• TheseinstructionsonlyapplytojacketedsystemsusingtheLaudaT1200TCUfortemperaturecontrol.

• Materials/Suppliesneeded:o TCUhosekitshippedwiththecontroltowero TCUcommunicationcableshippedwiththetower(● ● ●)o Coolantliquido FunnelusedforaddingtheCoolantLiquido Trayorbucketforcatchingfluidduringthepurgeprocess

• PleaserefertoLAUDATCUmanualprovidedifmoredetailsarerequired.

1. PlacetheTCUnexttotheS.U.B.andcontroltowerunit.2. Ensurethevalveforthedrainisclosed(seeFigure1).

Figure 1. TCU connection

Thermo Scientific 4 S.U.B. TK Jacketed Quick Start Guide

Quick Start Guide

3. Connections:(CablesandHosesprovided)a. ConnectoneoftheprovidedhosestotheoutletoftheTCU

andtotheinletoftheS.U.B.b. ConnecttheremaininghosetotheinletoftheTCUandthe

outletoftheS.U.B.c. Connectthecommunicationcable(● ● ●)fromthefrontof

theTCUtotherearofthetower.NotethattheconnectionontheTCUisbelowthedisplayandcanonlybeaccessedwhenthefaceistiltedup.

d. ConnectmainpowertoTCU.

4. Reservoir:a. Reservoirneedstobefilledtomid-levelwithcoolant/water

mix–seeLaudamanualfor(centermarkonthefluidlevelgage).AccesstofillthereservoirislocatedonthefrontoftheTCU.Fillslowlyandmonitorfillgaugeperiodically.

Figure 2. TCU fill level gauge at half full

Thermo Scientific 5 S.U.B. TK Jacketed Quick Start Guide

Quick Start Guide

5. PurgeTCUandJacket:a. Openvalves.Ensureinletandoutletvalvesonthejacketare

open.b. Turnonthemainpowerusingthetoggleswitchatthefrontof

theTCU.c. Onstartup,theTCUwillself-testandbegintopurgetheair

outoflinesandjacket.d. Duringthepurgeprocess,thereservoirlevelwilldropinorder

tofillthejacketoftheS.U.B.NOTE:VerifybypassvalveisnotrecirculatingfluidinTCU

e. Whenthelevelgetstoolow,theTCUwillsetoffanalarm.Repeatstep4andfillthereservoirtomid-levelandthealarmwillturnoffandthemachinewillcontinuetopurgeandfillthejacket.

f. Thisprocessmayneedtoberepeatedseveraltimesuntilthejacketisfullandmostoftheairisremovedfromthesystem.

g. Placeatrayorbucketunderneaththejacketventvalve.h. Keeptheventvalveopenuntilcoolingfluidcomingoutof

thevalveisfreeofair(TCUneedstoberunningduringthisprocess).Thismaytake1-2minutes.Thenclosethevalve.

i. DoafinalcheckonthefluidleveloftheTCUandensurethefluidlevelisatmid-level.

j. Ifnecessary,adjustdefaulttemperature.

CAUTION: Donotfillreservoirtotopoffilllevel,thecoolantmayneedroomforexpansion.

6. WhentheTCUisinitsfinallocation,rollthecontroltowerintoplaceovertheTCUanduptothestoplocatedinsidethetower.Notethatanaccessdoorislocatedonthefrontofthetowertoviewormanipulatethecontrols.

Figure 3. Jacket Purge Valve

Thermo Scientific 1 S.U.B. TK Jacketed Quick Start Guide

Quick Start Guide

2.1 Orientation and Location

Section 2 Hardware Installation

TheSingle-UseBioreactorhardwareisshippeddirectlyfromthemanufacturerandwillarrivecrated.Forunpackinginstructionsanddetailedcontentsofthecrate,pleaserefertotheuncratinginstructionsthatcamewiththeshipment.Besuretofollowtheunpackinginstructionsprovidedandretainallpackagingmaterials.

Tools Needed:• Allenwrenchprovidedinshippingdocumentationpacket• Adjustablewrenchneededforlevelingfeet(notsupplied)

a. Positionreactorandtowerindesiredlocationandlockwheelsensuringavailabilityofneededutilities(power,gas,etc.).Rightorlefthandorientationwasdeterminedattimeoforder.Ensurethattowerislocatedonthesamesideasthecableandtubingorganizer.

b. Beforerunningcables,notethatthecableandtubingorganizer

hasanadjustmentfeaturetokeepthecableslinedupwiththerearofthecontroltower.Thearmhasaslidethatcanextendandretractmanuallyusingtheclampingknob.

WARNING:Anyproceduresthatrequirethecontrolboxtobeopenshouldbeperformedwiththemainelectricaldisconnectinthelockedoutpositionandallpowersourcesremovedfromthecontrolbox.Foroperatorsafety,securethelocationoftheS.U.B.outersupportcontainerbydisablingtheswivelcastersbeforeservicing.

Figure 4. Cable and Tubing Organizer Figure 5. Cable and Tubing Organizer Adjustment Feature

Thermo Scientific 2 S.U.B. TK Jacketed Quick Start Guide

Quick Start Guide

2.2 Attaching Cables and Gas Lines

Figure 6. Back panel labeling

• ConnectMotorPowercableintodesignatedTowerconnector(● ● ●)• ConnectMotorFeedbackcableintodesignatedTowerconnector(● ● ●)• ConnectLoadCellcableintodesignatedTowerconnector(● ● ●)

• Usethethree(3)color-coded,labeledgaslinesandconnecteachlinetotherespectivecoloreddotsourceontheTower.

i. OverlaySparge–Black(●)ii. MicroSparge–Red(●)iii. MacroSparge-White(●)

• Servicegaspressureshouldbeaminimumof30psi,andamaximumof35psiforproperoperation.Verifythatgasconnectionsaresecureandtherearenoleaks(e.g.Snooporsimilarproducttodetectleaks).

• ConnecttheRTD(temperatureprobe)cable(● ● ●).Thiscableshouldbeinaboxthatwasshippedwiththetower.

ConnectthecorrespondingfacilitygassourcestothedesignatedconnectorsontheTower.Theconnectorsarefractional(inches),soifthefacilityusesmetriclines,simplyusetheadaptersprovidedintheplasticbaginsidetheTower(6mm.to1/4”adapters).

Thermo Scientific 3 S.U.B. TK Jacketed Quick Start Guide

Quick Start Guide

• Attachprobeclipstoprobewindow(frontoftheS.U.B.).TheprobeclipswereshippedwiththeS.U.B.unit.

Figure 7. 250 L S.U.B. TK System

Positionandattachvarioushardwareitems:• Securethetoolbrackettothetank.Thispositionisnotcritical,

butwouldbeconvenientnearthemotor.Placetheratchetandthecustomopenendwrenchinthetoolholder.Attachdriveshaft,asshown.

• Attachtheadjustableventfilterheaterbracketusingtheclampleverhandles.Positionthishardwaredirectlyoppositeofthemotor,asshown.

Thermo Scientific 4 S.U.B. TK Jacketed Quick Start Guide

Quick Start Guide

NOTE:Beforeconnectingcables,routethemthroughthetubingandcableorganizerfirst• IfyouintendtousetheFinesseTruFluorsingle-usesensors,route

theappropriatecablesfromtheTowerintothecablemanagementarmanddowntothesensorwindowareaontheS.U.B.ThesecableswillalreadybeattachedtotherearoftheControlTowerviaaliquidtightconnectionandhavethefollowingdesignation,DO-2andpH-2(Nocolorcode).

• Ifyouintendtousetraditionalre-usablesensingprobes,routetheappropriatecablesinthesamemannerasdescribedaboveforthesingle-usesensors.Ensuretheyarepluggedintothebackofthecontroltower(● DO-1●,● pH-1●).

• LocatethebagpressuresensorcableontheTower(● ● ●)androuteitviathecablemanagementarmanduptowardthetoparmofthetank.

Ifusingaventheatermakethefollowingconnections.• ConnectcabletolabeledVH-1POWER(● ●)powersourceon

Tower.• ConnectYellowThermocoupletolabeledconnectiononTower

VH-1FDBK(● ●)• Routecablethroughcablemanagementarmanduptoventheater

bracketsontopofS.U.B.unit.• Ifusingasecondventheater,useVH-2connections

VH-2POWER(● ● ●)andVH-2FDBK(● ● ●)androuteparallelwithVH-1cable.

Vent Filter Heater

Ifusingaresistiveheatermakethefollowingconnections.• ConnectcablelabeledRESHEATER(● ● ●)tothepower

sourceontheTower• ConnectcablelabeledHEATERPOWERIN(● ● ●)into

designatedtowerconnector.

Resistive Heater Power Connection

Thermo Scientific 5 S.U.B. TK Jacketed Quick Start Guide

Quick Start Guide

Beforeconnectingpower,verify,again,allgaslineswerecheckedandnotleaking.

• Connectmainpowercord(nocolorcode)tocorrectutilitysource.• ItisstronglyrecommendedthataUPS(UninterruptedPower

Supply)(● ● ●)beused.Thepurposeofthisconnectionistomaintainpowertothecontrolsduringapoweroutage.

• IfUPSpowerisnotavailableornotused,thentheUPSJumperconnectormustbeputinplaceoftheUPScable.

2.3 Power Connections

UPS Jumper

Figure 8. UPS Jumper

EnsuretheS.U.B.unitisinitsintendedposition.• Lockthevesselinplacebyloweringthelevelingfeet.Ensurethat

unitislevelbeforeoperation.• UsingtheAllenwrenchprovidedwithS.U.B.unitshipping

documents,removetheloadcellshippingplatesandsecuretheminthebracketattherearofthecontroltower.Therearetwo¼-20socketheadcapscrewsthatneedtoberemovedfromeachloadcellprotectionplate.Movethetowertodesiredpositionandlockinplaceandlevelusingthelevelingfeet.

2.4 Securing Position of Control Tower and

S.U.B.

Thermo Scientific 6 S.U.B. TK Jacketed Quick Start Guide

Quick Start Guide

• RefertoUser’sGuidefordetailedinstructionsoncalibrationsteps(loadcells,sensors,etc).

• Avirtualkeyboardiconislocatedatthebottomofthewindowdisplayfornotes,values,etc.

• StartbyturningontheMAINDISCONNECTswitchlocatedatthebackofthecontroltower.

• EnsuretheEmergencyStop(locatedatthefrontofthetower)isnotactivated(shouldbeintheoutposition).Ifitisengaged,thentwisttodisengage.NotethattheE-stoplightwillremainred,eventhoughitisdisengaged,untilthenextstephasbeencompleted.

• TurnpoweronbypressingthegreenStart/Re-setbuttonlocatedatthefrontofthetowerbehindtheoperatorscreen.E-stoplightwillthenturnoff.

• Notethatagreenoperationlight,locatedatthebackofthetower,willalsoturnon.

Themonitorcanbetiltedupanddownandrotatelefttoright.2.5 HMI (Monitor)

2.6 Power Up the System

2.7 System Will Begin Booting Up

Thermo Scientific 7 S.U.B. TK Jacketed Quick Start Guide

Quick Start Guide

Section 3 BPC Installation

1. CarefullyunpacktheS.U.B.BioProcessContainer(BPC)fromitstwoprotectivepolybags.

2. Uncoilandremoveprotectivepackagingfromthesparger,drain,andauxiliarylinesonthebottomoftheBPC.Don’tremovetheprotectivepackagingontopoftheBPCatthistime.

3. CarefullylowerthebottomendoftheBPCintotheS.U.B.withanytubingsetsonthetopsidelayingoverthesideuntilthebearingassemblyislevelwiththedriveshaftmotor.

4. Removetheprotectiveblackcapfromthebearingassemblyandslideintotheretentionmechanismontheagitationmotorandlockitintoplace.

Figure 9. Bearing port inserted into motor mount slot and clamp

Thermo Scientific 8 S.U.B. TK Jacketed Quick Start Guide

Quick Start Guide

5. Guidebottomtubinglinesetthroughtheirproperchannels:• Insertfritandopenpipespargerlinesthroughthespargeplate

(round,whiteplasticwithslots)first,beforelocatingitinthebaseofunit.

• Insertdrainlinethroughbottomfrontfacedirectlybelowagitationmotor.Hangthedrainlinecoilonthearmprovided.Alsoroutethesamplelinethroughthesnapinslotsonthedraincoilarm.

• AttachBPCpositioningtabs(bottomofchamber)ontothealignmentpinslocatedatthebaseoftheS.U.B.tank.Notethatthe50LBPCdoesnotrequirethisstepandisnotrecommended,buthastabholdersavailable,ifdesired.

• Insertsensors,KleenPaks,andauxiliarylinesetsthroughthe“probebeltopeningonthetank”.

6. Uncoverventfilters,wrapheatingblankets(ifused)aroundthefilters,attach/adjustventbracket,andmountventfiltersinbracket.

Figure 10. BPC tab holders used for proper BPC positioning

Figure 11. Filter being placed in bracket

Thermo Scientific 9 S.U.B. TK Jacketed Quick Start Guide

Quick Start Guide

7. Routetheoverlayspargeset(locatedatthetopoftheBPCmarked“OverlaySparge”)throughthecableandtubingorganizerarmtothetopofthemainbeamofthecableandtubingorganizerandconnecttothefittingmarkedOVERLAYSPARGE.Thiswillbethelinesetthatwillbeusedtopre-filltheBPCwithair,soensurethattheclamponthislinesetisreleased.

8. RoutetheMicroSpargeLine(labeled),locatedatthebottomoftheBPC,upthroughthemainbeamofthecableandtubingorganizerusingtheblacksnapinfeatures.ConnecttothecorrectgasfittinglabeledMICROSPARGE.Unclampthislinesetwhenreadytospargethroughfrit.

9. RouteMacroSpargeline(labeled),locatedatthebottomoftheBPC,runningparalleltotheMicroSpargeLine,usingthesamesnapinfeatures.ConnecttothecorrectgasfittinglabeledMACROSPARGE.Ensuretheclampisreleasedonthisline,aswellbeforeoperation.NOTE:Toreducetheriskoffiltermembranewetting,positioneachspargefilterabovethelowpointofthespargeline.

10.ConnectthepressuresensorcableontothesensorlocatedatthetopoftheBPC.

Figure 13. Pressure sensor

Figure 12. BPC linesets

Thermo Scientific 10 S.U.B. TK Jacketed Quick Start Guide

Quick Start Guide

11.EnsurethecablehasbeenroutedthroughthecableandtubingorganizerandconnecttothefittinglocatedattherearofthecontrolcabinetlabeledBAGPRESS(● ● ●).

12. IMPORTANTDisengageanyclampsonthelinesetfortheexhaustfilter(s).Iftheclampsareleftengaged,theBPCcouldburstwhenfillingitwithairorliquid.

13.Positionandcloseabarclamp(PartNo.SV20664.01)onthebottomdrainlineascloseaspossibletotheBPCport(Figure14).Alsoplacebarclampsonprobeportsintendedforconventionalprobes(Figure15).

14.InsertRTDorselectedtemperaturesensorintothermo-well(Figure16,Figure17).• Placesmallamountofglycerol(0.5mL)inwelltoaidinheat

transfer.• Sensorshouldbeinserteduntilbaseofprobemeetsthemouth

ofthethermo-well.• Securebytwistingtheluerlockcollar.Thethermo-wellwill

stretchslightlywhenRTDisseated.

Figure 16

Figure 14

Figure 17

Figure 3.15

Thermo Scientific 11 S.U.B. TK Jacketed Quick Start Guide

Quick Start Guide

Withthesoftwarebootedup,clickonthe“HOME”buttonontheupperleftsideofthescreen.Inthetoprighthandcornerofthescreen,clickontheicon“Guest.”

ThenclickontheUserpulldownmenuandselect“Administrator.”

EnterthecorrectPassword,whichis“admin.”

TotypeinformationwithoutpluggingaUSBkeyboardintothecontroltower,clickontheminiaturekeyboardiconlocatedatthebottommiddleofthescreen.Thiswillbringupatouchscreenkeyboard.Whenfinishedusingthetouchscreenkeyboardjustclickontheupperrighthandcornertocloseit.Note,youmustbringupthekeyboardFIRSTbeforeopeningadropdownmenu.Alsonotethatthekeyboardcanbemovedandrepositioned.

Figure 18 Initial TruBio View With Log On Breakout

Section 4 Software Start Up

Thermo Scientific 12 S.U.B. TK Jacketed Quick Start Guide

Quick Start Guide

Nowtheheadingshouldread“Administrator.”

ThenextstepistoloadthecorrectconfigurationfilefortheSUBunit.Clickonthe“Configure”buttonontheleftsideofthescreen.

NOTE:youcanalsoclickontheyellow“LoadConfigurationFile”buttonwhenfirstturningonthesystem.

Clickthetabonthetopthatreads“File.”

SelecttheappropriateS.U.B.TKsystemtobeusedbyclickingtohighlightit.

• ThermoTurnkey250LElectricSUB• ThermoTurnkey250LJacketedSUB• ThermoTurnkey50LElectricSUB• ThermoTurnkey50LJacketedSUB

Loadthenewconfigurationbyclickingthe“Load”buttonontheright.

Select“Yes”whenthepopupwindowappears.Thescreenautomaticallyjumpstothe“Home”displaywindow.

Figure 19 Configure Files View

Thermo Scientific 13 S.U.B. TK Jacketed Quick Start Guide

Quick Start Guide

Figure 20 “Home” Monitor View

Thermo Scientific 14 S.U.B. TK Jacketed Quick Start Guide

Quick Start Guide

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