Hydro Enterprises, Inc

Hydro Enterprises, Inc.Hydro Enterprises, Inc.

A Modern Breakthrough For Life's A Modern Breakthrough For Life's Most Precious Resource:Most Precious Resource:

WaterWater

8-1-2007 / 1 of 33 slides

Plasma Activated Water Technology

Will be considered one of the most modern day marvels of this century.

. Our Plasma Activated Water Technology will make water monomolecular, remove the harmful effects of bacteria, viruses and other pollutants created by man, and if you desire can enhance the structure of water for your benefit.

This is done with….> NO Chemicals

> Little or NO Salts

> NO Harmful ProcessesNatures Most Precious Resource - Water

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Plasma Activated Water (PAW) Technology?

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Primary Benefits1. Makes water monomolecular in structure. The monomolecular structure is stable for

a sufficient period of time to be used for thousands of applications.

2. Provides for the ability to kill bacteria and viruses contained in water or fluids. Several tests have been completed including wastewater treatment tests, that proved 99.9% kill within the water being processed.

3. Provides for the ability to kill any bacteria and virus on any surface upon contact. The actual time to obtain a 6 log kill is on contact, not in seconds. Several tests have proven a 99.9% kill of bacteria and viruses such as e-coli, bacillus subtilis, staph, poliovirus, and rotavirus.

4. The PAW Technology has the ability to facilitate the removal of contaminants from any water source.

5. Provides the ability to make High pH water, up to 12.5 from any water source with an Oxygen Reduction Potential (ORP) of -600 mV and Low pH water as low as 1.5 with an ORP of + 1,200 mV.


Improves plant and animal growth rates Eliminates / Reduces contaminates Extends product shelf life by 7 to 14 days. Eliminates / Reduces bacteria and other contaminates in food processing Improves dairy herd milk production up to 28% Improves insecticide effectiveness – Use less chemicals to achieve same

results


Agriculture

Crops – Dairy - Poultry – Beef – Pork Production and Processing

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Plasma Activated WaterTechnology

Agriculture Eliminates bacteria and viruses

on produce for a healthier food source

Studies have shown that fruits and vegetables washed with a low pH water will eliminate or reduce bacteria such as E.Coli, Pseudomonas, Staphylococcus, Salmonella and many others.

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Agriculture Improves plant growth

Test Period Was For Approximately One Month Ending June 19, 2007

Example: Cow Peas

The Plant on Left: Was grown using High pH Water. The plant reached over twelve inches in height within one month.

The Plant on Right:Was from the same seed lot, and given the same volume of ordinary tap water which grew to about one half the size of the other plant that was given High pH water in one month

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Agriculture Improves plant growth Eliminates bacteria and viruses in plants for

a healthier food source

Extends fresh fruits and vegetables shelf life by 7 to 14 days.

This test was completed over a seven day period. All the strawberries were left exposed at room temperature.

The strawberry in the center was treated with High pH water, the others with tap water.

Not Treated Not Treated

Test Was Completed Over a 7 Day Period in Open Air and at Room Temperature. 8

Sprayed With High pH Water


Industrial Sterilization


Early test results have demonstrated that Hydro Enterprises low pH Plasma Activated Water can eliminate or reduce bacteria and viruses, including but not limited to Bacillus Subtilis, E Coli, Staphylococcus Aureus, Salmonella, Coliform MPN, Poliovirus, Pseudomonas, Rotavirus and MS2 Bacteriophage

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Manufacturing


Any manufacturing company that needs water with a controlled pH, sterilization requirement or monomolecular water structure can benefit greatly by utilizing Plasma Activated Water Technology in their manufacturing process.


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How Does This Work ? #1

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This RF plasma process is unique because it is a non-chemical method of treatment. The RF plasma technology breaks the clusters of molecules in the water producing monomolecular water. The process uses a gas mixture of oxidizing and/or reducing agents that are ionized creating plasma inside of a quartz vessel. This newly created plasma contains ionization properties including charged particles, ultrasound modulation and ultra-violet radiation.



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The water source is then processed through the RF plasma reactor. The water crosses an electric/magnetic field and is also exposed to ultrasound and ultraviolet radiation from the RF plasma. This treatment destroys the molecular clusters contained in the water. The number of molecules in water clusters decrease from 10-24 to 1-3. Water activity increases in proportion to the decrease of water clusters. The monomolecular/activated water now has small sized water molecules allowing it to penetrate the membranes and pores of animals, plants and processed food.



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Combined with a lower pH level, the monomolecular water becomes even a more powerful anti-bacterial agent making the water more acidic. What makes monomolecular low pH water one of the most effective and safe anti-bacterial tools on the market today is that this technology produces low pH water that is not harmful to humans, animals or plants but yet this water is one of the most effective anti bacteria products on earth. Even with the monomolecular water being at a low pH it will not burn human skin and can be disposed of without special handling.

O HH

H2O- moleculeH2O

H2O

H2O

H2O

H2O

H2OH2O

H2O

H2O

H2O

H2O

WATER

pH = 7 – 8ORP = +500 mVSize: 10 to 24 mol.

PLA

SMA D

C-Field

H2O H2O

H2O H2O

H2O

H2O

H2O

H2O

H2OH2O

(OH)- H+

H+ (OH)-

(OH)- H+

H+ (OH)-

Na+

K+

Ca+

Mg2+

Cl-

HCO3-

(OH)- predominant pH : -600 +100mV

H+ - predominant pH: +800 +1200 mV

ORP – (e –activity)Ph –Hydrogen index(Concentration ratio H3O+ & (OH)-)

How Water Is Effected With Plasma Activated

Water Technology

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RF Power SupplyBasic Frequency: 0.44 MHz – 40.68 MHz

Modulation Frequency: 10 – 35 kHzRF Pulse: 10 -40 kV

Pulse width: 5 – 45 microseconds

Gas Bubble

Water/Liquid

Plasma

Blown Gas Bubble

Gas System(option)

Water Flow

Shock waves/Ultrasound

Ozone UV (<200 nm)

E-field

What Happens As Water Passes Through The Plasma Activated Water System15


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Source Water Input

High pH Water Output

Low pH Water Output

PAW Technology provides for the ability to raise or lower the pH of water to meet your needs. As water passes through the Radio Frequency Plasma Reactor, the water can be split into two types of water, each having their own benefits. One being a high pH activated water and the other a low pH activated water.

Improves health of humans, animals and plants Increases plant and animal production Reduce or eliminate NO2, NO3, CL, etc.

Examples of Benefits of High & Low pH Water

High pHSanitizer/anti-bacterial/powerful disinfectantNo added chemicals like CL or TSPKills bacteria and viruses.

Low pH

LOW TEMPERATURE PLASMA

ISOTHERMICAL PLASMA NON-ISOTHERMICAL PLASMA

Arc Discharge RF Discharge

Microwave Discharge

Glow Discharge Corona Discharge

Te ~ Ti ~ Tg ~= 10,000 K

K = K0 exp(- Wakt./kT)A2/(1-A2) x p = 3.32 x 10-2 x T5/2exp(-Wion/kT)

Te >> Ti ~ Tg Te = 30,000 KTi ~ Tg ~ 375K

RF DischargeLow Pressure

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Water Properties

ParameterpH

Conductivity

T. Dissolved Solids

TDS (summation)

MBAS

Color

Odor

Turbidity

Hardness

EPA Method150.1

120.1

160.1

…….

425.1

110.1

140.1

180.1

130.2

Detection Limit0.1 units

0.1 hOm/cm

5 mg/l

…mg/l

0.05 mg/l

0.5 unit

1.0 TON

0.5 NTU

0.5 mg/l

Tap Water8.3

244

388

391

0.07

14

<1.0

<0.5

189

Activated Water2.7

330

378

408

0.10

10

<1.0

<0.5

133

Calcium

Magnesium

Sodium

Potassium

Cations

Copper

Iron

Manganese

200.7

200.7

200.7

200.7

…….

200.8

200.7

200.8

0.02 mg/l

0.1 mg/l

0.1 mg/l

0.02 mg/l

…meq/l

0.01 mg/l

0.03 mg/l

0.01 mg/l

42

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53

3.4

5.9

<0.01

0.09

<0.01

27

15

84

1.6

6.3

0.01

0.06

<0.01

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p

G

IDEC (micro SMART)

RF/DCPower Supply

pH/ORP

1

2

LP /drain By-product(20%)

HP product (80%)

Pump5000 GPH

To Application

Water HoldingTank

SelfCleaningSystem(Auto/Man)

Filter

Water Source

Plasma Activated Water Treatment System

pH: 6.8- 7.5

Key

A.) Water is supplied

B.) Passes through the plasma activated water treatment system

C.) Exits to either the low pH (C-1) or high pH (C-2) outlets

A

B

C-1

C-2

The holding tank can be used by either low, high pH output or both depending on the volume and requirements needed.

NOTE:20

General Water, Disinfection and Sterilization Uses

Plasma Technology

Wastewater Treatment


With the benefits of eliminating or greatly reducing bacteria, viruses and other harmful contaminants in water, this purification system could be used in many water treatment applications. With capabilities of purifying water at a rate of up to 100,000 gallons per hour, this technology offers a very cost effective means of wastewater treatment.

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TYPICAL WASTE WATER TREATMENT SYSTEM

INCOMINGSEWAGE

DIGESTER

Option: CHLORINATION IF REQUESTED

HEADWORKS

Bar Screens: Remove wood,Plastics and rags

SEDIMENTATIONCapture sand and solids

PRIMARY TREATMENTcoagulation Sludge

Tre

ate

d W

ate

r

Methane

De-Watering

Re

-use

d B

io-so

lids

ENERGY RECOVERY

Water Re-Use

PLASMA SYSTEMAnti-bacterial treatment, pH Control

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Option: Reverse Osmosis or Filtration System

Clear Water

MICROBIOLOGY LABORATORY, ENVIRONMENTAL MONITORING DIVISION

WASTEWATER 5 MILE EFFLUENT Sample Date: 30-Jul-03Analysis Date: 07/31/03

Total Coli-form Fecal Coli-form EnteroRead by CES CES AMA

Time 13:25 13:25 14:40

Before Treatment, CFU/100ml

2,400,000 4,800,000 2,000

After Treatment, CFU/100ml

< 1 < 1 < 1

Southern California Laboratory – SILLIKER , Inc.

Sample Date: 30-Jul-03Analysis Date: 08/01/03

SCA-15205202-0 Coli-Forms Heterotrophic Plate Count

Note

Water Before Treatment > 1,600,000 / g 34,000 / ml 5 tube MPN

Water After Treatment < 2 / g 60 / ml

Sludge Before Treatment

170,000 / g 5 tube MPN

Sludge After Treatment 17,000 / g23

Sterilization by steam or dry heat is not useful to sterilize materials that are adversely affected by such heat or steam

Ethylene Oxide E2H4O gas: suffer from residues on the articles to be sterilized, which may have adverse effects

Radiation Sterilization: limited to large industrial applications because of the large investment to build the gamma-radiation facilities, safe operation and change of bulk properties of non-metallic materials, especially food.

Plasma Sterilization: require special equipment (vacuum chamber, pumps, gas) and qualified operator.

Plasma activated water sterilization: no chemicals; safe; disposable; simple.

Sterilization

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PAW Is Effective Against:Viruses and bacterial Spores: Bacillus Subtilis, E Coli, Staphylococcus Aureus, Salmonella, Coliform MPN, Poliovirus, Pseudomonas, Rotavirus and MS2 Bacteriophage

Complex structures such as multilayer Albumen Coats

Dental bacteria such as Polysaccharide Exopolymers and Bio-films

PAW Is Effective With:

Species with high sporicidal conyesounds such as OH,H,O

Highly reactive components such as H2 O2, CLO, HOCL, CL, H+, etc

Table 1. BG Spore Strip Test SummarySolution Number of Spray

PassesHydro Enterprises provided activated water.

This number represents the amount of decrease in spores present. The term “BG spores” refers to Bacillus Subtilis, which is often used as a surrogate for Anthrax.

2 6 10

PAW 3 – 6log >6 log

>6 log

Sprayer (minimized fluid consumption)-Nozzle orientation: -moved along the surface at a speed of 1 feet/sec and 2 feet from the surface-Validation: sampling with swabs, cultured.

1925

Spore Test Strip Test

Fluid Decontaminant Sporalized Bacteria

Bacillus anthracis(Ba)

Sporalized Bacteria Simulant

Bacillus subtilis var globigii

(BG)

Viral SimulantMS2

Product Comments

Easy DECON >6 log >6 log >4 log The MSDS for this solution should be read thoroughly before use. It is less caustic than most other solutions. This solution is produced in the United States and is available through EnviroFoam.

Hydrogen Peroxide 3%

>6 log >6 log >4 log Use carefully and read the MSDS thoroughly before use. The easy availability and handling of this fluid make it very appealing as a solution. The solution has a nearly unlimited shelf-life.

Hypochlorous Acid (0.5%)

>6 log >6 log >7 log Use carefully and read the MSDS thoroughly before use. The easy availability of the foundation for this fluid, bleach, makes it appealing as a solution but the method to convert simple bleach into a properly mixed hypochlorous acid solution is somewhat complex. Solution has a limited shelf-life, but it should be used within two-three hours after preparation.

Antibacterial Water N/A >6 log >4 log Contact Hydro Enterprises for further information.

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Test Results

Each Sample Represent 30 Second Shake With Either Low pH Water, High pH

Water or TSP Solution In Turkey Processing Plant

CFU's MESO PHSYC ECOLI TCOL STAPH YM Lactics SAL LIST

LO pH 3,929 2,821 43 67 57 36 79 0 1

HI pH 6,586 3,671 39 80 147 53 83 0 1

BUFFER 17,314 9,900 1,333 1,719 91 101 360 0 4

TSP/BUF 9,400 8,271 383 533 149 66 141 0 2

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ACTIVATED H20 TEST

Test MESO PHSYC ECOLI TCOL LACTICS SAL

Egg Floor < 10 < 10 < 10 < 10 < 10 Neg.

Egg - Nest < 10 < 10 < 10 < 10 < 10 < 10

Final LP water

< 10 < 10 < 10 < 10 < 10 Not tested

Final HP Water

370 490 < 10 30 20 Not tested

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The natural casings: experimental dataResults:1. Casing Sample out of the barrel………….. 510,000 col/g2. Casing Sample just rinsed with tap water…180,000 col/g3. Casing treated with Activated water -L2.2 1,500 col/g4. Casing treated with Activated water -L5.0 1,600 col/g

Notes: 1.Treatment time = 45 sec2. Analyzed for aerobic plate count 3. The use of the activated water resulted in a 2 log reduction of the bacteria load on the casings

SANITATION

Sausage Manufacture

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•Why Is It safe ?

Through the process the pH and ORP of water is increased or decreased to meet your needs, along with eliminating or reducing bacteria, viruses and other contaminants. The low pH water does not have the typical burn effects found with fluids at very low pH levels. This patented technology coupled with industry standard filtration systems produce clean Plasma Activated Water for your needs.

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Electro-Chemical Magnetic Field

Freeze and Thaw Action of Acoustic Oscillation

Heat Impact under the Pressure

Competitive Technology Used To Create Activated

Water

Our Plasma Activated Water Technology is the most versatile, cost effective and reliable system available in the world today.

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Hydro Enterprises, Inc.

Water Treatment Equipment available from 100 gallons per hour up to 100,000 gallons per hour.

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10 Mclaren Suite B

Irvine, CA. 92618

(800) 617-2088

Manufacturing Agriculture

Bottled Water

Bio Chemical Sterilization

Medical Sterilization

Industrial Sterilization

Health

Waste Water Treatment

Emergency Water TreatmentGeneral Water Improvements

Life’s Most Precious Resource

Water


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Hydro Enterprises, Inc