Hplc Column Phenomenex Luna

8/19/2019 Hplc Column Phenomenex Luna

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®

The World’s #1 HPLC Column


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®


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TABLE OF CONTENTS

Overview ....................................................................................... 2

Reproducibility Validated.............................................................. 4

pH Stability 1.5-10 ........................................................................ 6

Luna C18, C8, C5 ......................................................................... 8

Luna Phenyl-Hexyl...................................................................... 18

Luna Cyano [CN] ........................................................................ 20

Luna Amino [NH2] ....................................................................... 22

Luna LC/MS and ........................................................................ 24

Combinatorial Chemistry

Luna Capillary LC ....................................................................... 25

Luna Preparative LC ................................................................... 26

Bonded Phases ..........................................................................27Ordering Information ..................................................................28


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Wouldn't you like to boast about the performance of your HPLC

column? Many Luna® customers do. Our passion for manufacturing

the best HPLC columns, has resulted in Luna - one of the World's

most popular columns. Luna represents some of the highest quality

and performance standards in HPLC. Take a moment to explore

Luna, we are certain you will discover amazing HPLC column

performance like so many of our customers.

Superior Silica For Long Column Lifetimes

Silica quality is not simply the result of Luna's 99.999% purity.

Meticulous care is given to quality control over silica smoothness,

pore structure and pore consistency leading to a highly advanced

HPLC silica with:

• Low percentage of "fines" from damaged silica

particles stabilizing the column bed and leading

to lower backpressures and enhanced column

performance and lifetimes

• High column bed stability enhanced by

particle shape uniformity

Overview

Phases to separate any compound

LUNA ® : Si, C5, C8, C8(2), C18, C18(2), Phenyl-Hexyl, CN, NH2

Incredible Silica Smoothness

Luna silica is very smooth and spherical, as illustrated in the Luna

versus Zorbax® silica comparisons below. The likelihood of silica

particle shearing and breakage during bonding and packing is very

low; thus, Luna columns have high efficiencies and long column

lifetimes.

2 Phenomenex Tel. 310.212.0555 Fax 310.328.7768 Web www.phenomenex.com


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pH Stability: 1.5 to 10.0*

Luna was one of the first columns to provide greater flexibility and

control in method development at pH extremes. More importantly,

Luna provides good peak shape independent of mobile phase pH.

Reproducibility Validated

A Materials Validation Document showing the test results for the

batch of material and the individual column accompanies each

Luna column. This data ensures column-to-column and batch-to-

batch reproducibility.

Versatile Configurations

Capillary-LC to preparative/process chromatography columns and

bulk material are available to meet any separation need.

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Reproducibility ValidatedIt is tough to meet Luna quality standards. The Tanaka plots below

illustrate the reproducibility of 13 different quality control parameters

for 9 batches of Luna material - nearly no variation is observed.

Each batch represents hours of quality control testing for over 25

different parameters. We know chromatographers rely on Luna for

rugged, reproducible methods worldwide; therefore, an extensive

Materials Validation Document (MVD) accompanying each column

confirms consistent column specifications and consistent

performance.

Reliable Performance

Almost no variation is observed among the batches of Luna. Figure

1 shows quality control test data designed to monitor the slightest

differences that may affect reproducibility - particle shape and

smoothness, porosimetry, bonding consistency and pH stability.

Figure 2 shows bonding reproducibility test data from 9 Luna

batches. Since differences in bonded phase coverage can show

selectivity changes, each Luna batch undergoes 6 tests for bonding

reproducibility.

Figure 1 - SilicaReproducibility. Batch No:

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Column to Column Reproducibility

The chromatograms in Figure 3 show consistency of inertness (blue)

and hydrophobicity (red) for Luna 5u C18(2) columns from 9 different

batches. Almost no variation is observed.

Figure 3 - Column-to-columnreproducibility for 9 batches of Luna

Figure 2 - Bonded PhaseReproducibility. Batch No:

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Luna was one of the first columns to provide an extended pH range

of 1.5 to 10* for most of its phases (Figure 4). The broad pH stability

range is partly achieved by the density of the bonded phase. Luna

C18(2) columns are exhaustively bonded to show great peak shape

independent of pH, indicating free silanols are well shielded from

the analyte compounds (Figure 5). Try Luna! It will consistently

provide good peak asymmetry across a wide pH range.

pH Stability: 1.5 - 10.0

Figure 4 - Efficiency and peak asymmetry stability at pH 10.0 and 1.5 forLUNA ® 5µ C18(2)

Hours of Exposure

Hours of Exposure

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Sharp Peaks For Basic Compounds at High pH

With Luna columns it is possible to work above the pKb of many

basic compounds, with the compound in a "neutral charge state",

thus the basic compound becomes more hydrophobic (less polar).

This can result in improved retention, peak shape, and

reproducibility. Figure 6 illustrates how a pH adjustment can be

used to alter selectivity and retention. A wide operational pH range

extending to 10.0 is useful for basic compounds.

pH 1.25

pH 5.0

pH 7.0

pH 9.0

pH 3.0

pH 10.0

Figure 6-Optimizing resolution and selectivity with mobile phase pH increases methoddevelopment flexibility.

Figure 5 - Average peak shape over pH range: Waters ®

Symmetry® 5µ C18 = 2.26, Phenomenex® LUNA ® 5µ C18(2) = 1.43

2 3 4 5 6 7 8 9

4

3

2

1

0

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Luna has found a place as one of the World's top reversed phase

columns because it ensures two important chromatographic

properties: resolution and peak shape. The high efficiencies and

bonded phase surface coverage provide for sharp peaks. Whether

you need a column for USP methods or just general method

development, Luna C18(2) and C8(2) should be your first choice

every time. The result:

• Free exposed silanols virtually eliminated

by complete bonding and endcapping

• Sharp peak shape for good method sensitivity

• pH stable from 1.5 to 10.0 for over 10000 hours

Luna C18(2), C8(2), C5

Phenomenex®

LUNA ® 5µ C18(2)

Waters®

Symmetry ® 5µ C18

Phenomenex®

LUNA ® 5µ C18(2)

Waters®

XTerra™ 5µ RP18

PEAK ASYMMETRY: A COMPARISON OF C18 COLUMNS

The proof is in the peaks - see for yourself.

8 Phenomenex Tel. 310.212.0555 Fax 310.328.7768 Web www.phenomenex .com


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POLAR, ACIDIC DRUGS

Phenomenex® LUNA ® 3µ C18(2)Waters® Symmetry ® 3.5µ C18

NON-POLAR, BASIC DRUGS

Phenomenex® LUNA ® 5µ C8 Agilent Technologies® Zorbax® 5µ XDB-C8™

BASIC COMPOUNDS

Phenomenex® LUNA ® 5µ C18(2)Macherey Nagel® Nucleosil® 5µ C18

HYDROPHOBIC, ACIDIC COMPOUNDS

Phenomenex® LUNA ® 5µ C18(2)Thermo Hypersil® HyPURITY ™ Elite 5µ C18

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DIET SODA POP - AND -ACIDS IN HOP EXTRACT

CAFFEINE AND METABOLITES

1

23

4

5

0 5 10 15 20 min

METHYLATED XANTHINES

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ESTRADIOL-USP METHOD COCAINE-NARCOTIC DRUG

PHENYLEPHRINE HYDROCHLORIDE INJECTION-USP METHOD HYDROCORTISONE CREAM-USP METHOD



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0 2 4 6 8 10 12 14 16

3

2

4

5

6

7

8

9

10

11

12

13

14

1

EPA 8330 - EXPLOSIVES EPA 8310 - POLYNUCLEAR AROMATIC HYDROCARBONS (PAH’s)

COLD MEDICINE

GINGER PUNGENTS



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POLYAMINES WATER SOLUBLE VITAMINS

PHARMACEUTICAL PRESERVATIVES

PYRONES



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MINOXIDIL-USP METHOD LORAZEPAM TABLETS-USP METHOD

HORMONES

IMPIRAMINE-USP METHOD

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FATTY ACIDS

0 2 4 6 8 10 12 14

1

2

3

4 5

6

7

8

min

NAPROXEN TABLETS-USP METHOD

GUAIFENESIN TABLETS-MODIFIED USP METHOD

ALBUTEROL TABLETS-USP MEHOD

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SUN SCREENS CEPHALOSPORIN ANTIBIOTICS

PENICILLIN ANTIBIOTICS

ANTIOXIDANTS

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SAW PALMETO BERRY, P-BROMOPHENACYL ESTERS ACETAMINOPHEN, USP METHOD

CAPSAICINCYCLOSPORIN - IMMUNOSUPPRESSANTS

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Luna Phenyl-HexylEngineered for Stability

The patented Luna Phenyl-Hexyl is a reproducible, extremely stable

phenyl phase. Most other phenyl phases use a short propyl (3

carbon) linker, which limits the phase stability. This patented phase

uses a hexyl (6 carbon) linker to attach the phenyl group to the

silica surface. The result:

• Highly reproducible phenyl phase

• Dual selectivity of both phenyl phase

and a short alkyl phase

(such as a C8)• Excellent retention of amine and polar

aromatic compounds

• 1.5 to 10 pH stability for over 6000 hours

ANTIBACTERIA LS: COMPARISON OF PHENYL COLU MNS

5 10 15 20 min

1

2

3

4

5

6

7

8

Waters® Spherisorb® 5µ Phenyl

Phenomenex® LUNA ® 5µ Phenyl-Hexyl Agilent Technologies® Zorbax® 5µ Phenyl

Agilent Technologies® Zorbax® 5µ SB-Phenyl

2.5 5.0 7.5 10.0 12.5 15.0 17.5 min

1, 2

3

4

7

5,6

8

20.0



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FOOD ADDITIVESCOUGH AND COLD-USP METHOD

CHLOROBENZENES COUGH AND COLD MEDICINE

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Proven Reproducibility

Luna CN was developed to provide reproducibile chromatography

from run-to-run, column-to-column and batch-to-batch. Luna’s

high-purity silica provides a ridged and dense column bed that

allows for improved CN bonding techniques to make a stable CN

phase. The result:

• One of the most stable CN columns under both reversed

phase or normal phase conditions.

• Reproducible from run-to-run, column-to-column,

batch-to-batch.• pH stable from 1.5 to 7.0

Luna Cyano (CN)Column-to-Column Reproducibility Batch-to-Batch Reproducibility

PHTHALATE ESTERS: A COMPARSION OF CN COLUMNS

Phenomenex® LUNA ® 5µ CN Waters® Spherisorb® 5µ CN Agilent Technologies®

Zorbax® 5µ SB-CN

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ORGANOCHLORINE PESTICIDES BENZALKONIUM CHLORIDE

RHODAMINE DYES

EXPLOSIVES



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Developed for Ruggedness

Luna Amino was developed to provide improved amino column

lifetime. Column life for most amino columns can be problematic

as the amino bonding easily strips off the silica. Luna Amino,

however, shows good bonded phase stability under both normal

and reversed phase modes and across a pH range of 1.5 to 11.0.

Such a broad pH range indicates the bonded phase ruggedness

and the density of the bonded phase coverage. The result:

• Long lifetimes and low phase bleed for more

reproducible methods• Excellent retention of simple sugars, complex

sugars, sugar alcohols by reverse phase conditions

and hydrogen bonding compounds under

normal phase conditions

• pH stable from 1.5 to 11.0

• Stable in 100% aqueous mobile phases

Luna Amino (NH2 )

SIMPLE SUGARS

STABILITY IN 100% AQUEOUS MOBILE PHASE

STABILITY AT PH 11.0



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SUGAR ALCOHOLS

NUCLEIC ACID BASES

COMPLEX SUGARS

STEROIDS



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LC/MS & Combinatorial Chemistry Luna materials are highly recommended for LC/MS and high-

throughput screening. Due to Luna’s stability from pH 1.5 to 10, a

broad range of volatile, MS-compatible mobile phase modifiers

are available for LC/MS applications. High loading capacity is

possible due to high pore volume and optimum pore size distribution

of Luna silica. Our proprietary bonding procedure produces uniform

bonded phase coverage excellent for rapid mobile phase re-

equilibration. The result:

• Sharp peak shape

• High loading capacity• Low phase bleed

• Rapid re-equilibration

PEAK SYMMETRY IN MS-COMPATIBLE BUFFERS

PERFORMANCE IN MS-COMPATIBLE BUFFER

LC/MS ANALYSIS OF ANTICONVULSANTS



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Capillary ColumnsHigh Efficiency and High Sensitivity

Luna capillary columns have the same characteristics as their

respective analytical-size columns but provide added sensitivity

for microliter sample volumes. The incredible smoothness and

sphericity of Luna silica results in a high efficiency capillary-LC

column with a stable column bed that yield long column life. Our

high-purity silica combined with proprietary bonding techniques

produce a dense bonded phase shielding against silanol

interactions. With little secondary silanol interaction, Luna capillary-

LC columns yield terrific peak shape, an essential criteria for mass

limited samples.

The result:

• High efficiency 0.30, 0.50 and 0.70mm ID

glass-lined columns

• Exceptional sensitivity for low volume and mass

limited samples

• Consistent performance under LC/MS compatible

and universal gradient mobile phase conditions

• 1 / 8 inch (3mm) OD tube for robust, durable columns

-LACTOGLOBULIN TRYPTIC DIGEST

-LACTOGLOBULIN (OXIDIZED) TRYPTIC DIGEST



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Less Material Required

Luna materials have extremely low particle densiti es meaning less

material is required to pack a given volume. For large-scale

preparative systems, such as production or process methods, this

can have an enormous impact on long-term operating costs. For

example, packing a 50L column would require up to 20% less Luna

media than the leading competitive media.

Exceptional Lifetimes

The extraordinary silica and bonding technology at the heart of

Luna provides exceptional lifetime. The rigid silica resists crushing,improving the chromatographic performance over the life of the

column.

Quick, Direct Scale-Up

Luna preparative media can provide nearly the same

chromatographic behavior as a Luna analytical column. Method

scale-up is simple. Luna 3µ C18(2) material used in the analytical

separation can be switched to Luna 15µ C18(2) material to obtain

similar separation and resolution with minimal changes in the

separation method. Furthermore, the same smooth silica surface,

high bonded phase coverage and pH stability available in analytical

Luna materials is inherently present in all preparative Luna materials.

High Loadability

Luna packing materials provide high surface area silica withoptimized pore size and pore size distribution and a high pore

volume that allows extremely high loading capacity.

Preparative LC

50 L

20L

5L

0.5L

36.4 kg

27.2 kg

14.6 kg

10.9 kg

3.6 kg

0.7 kg

2.7 kg

0.5 kg

0.4 kg

0.3 kg

Column Volume BulkMaterial

20% LESSMATERIAL REQUIRED

Luna® 10u C18(2) 100A

Leading 10u C18 100A

1L

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Bonded Phases

C8(2)

Optimized for maximum efficiency, peak shape and resolution.

Significantly improved performance over traditional C8 phases

due to high surface coverage. Slightly lower carbon load thanoriginal Luna C8.

C5

Reversed-phase chemistry that offers greater hydrolytic stability

and an alternative selectivity over the classic C4 phase.

Highly hydrophobic compounds. Good alternative

to C8 or C18 when less retention is desired.

Great starting point for methods development of

pharmaceuticals, nucleotides and polar compounds.

Excellent for highly aqueous and LC/MS applications.

C8

Original C8 phase. Highly retentive; optimized for long lifetime at

extremely high pH levels (pH >10.0).

A ”workhor se” for extre mely high pH appl icat ions

(pH >10.0), or when a more retentive C8 is desired.

C18(2)

Optimized for maximum efficiency, excellent peak shape and

resolution; slightly lower carbon load than original Luna C18.

C18

Original LUNA C18 phase. Maximum retention, optimized for long

lifetime at extremely high pH levels (pH >10.0)

A ”workhorse” for extremely high pH applicat ions

(>10.0), or when maximum retention is desired.

Phenyl-Hexyl

Novel (patented) phenyl phase which employs a hexyl linker, as

opposed to the traditional propyl chain. Stable under all conditions

as well as alternative selectivity to most phenyl phases.

Unique selectivity for very polar and aromatic

compounds when used in methanol containing mobile

phases.

CN

One of the best performing CN phases available. Can be used in

reversed- or normal-phase modes. Based on same 99.999% pure

silica as all Luna phases.

Excellent for improving the retention of polar

compounds. Extremely rapid equilibration makes it

ideal for rapid screening and gradient applications.

A C18 phase for virtuall y all HPLC appl icat ions .

Range of particle sizes offers amazing versatility for

capillary-LC/MS, to prep and process scale applications.

DESCRIPTION APPLICATION

NH2

Possibly the most rugged and reproducible NH2phase for reversed

or normal phase separations. Stable from pH 1.5 to 11.0 and

under 100% aqueous conditions.

Excellent for reversed-phase analysis of sugars, sugar

alcohols and anionic compounds or for hydrogen

bonding compounds under normal phase.

LUNA PHASE Particle Pore Surface Carbon Bonded pH StabilitySize (µ) Size Area Load Phase

(Å) (m2 /g) (%) Coverage(µmole/m2)

Silica(2) 3,5,10,15 100 400 - - 2.0 - 7.5

C5 5,10 100 440 12.5 7.85 1.5 - 10

C8 5,10 100 440 14.75 5.50 1.5 - 10

C8(2) 3,5,10, 100 400 13.5 5.50 1.5 - 10

10-PREP,15

C18 5,10 100 440 19.0 3.00 1.5 - 10

C18(2) 3,5,10, 100 400 17.5 3.00 1.5 - 10

10-PREP,15

Phenyl-Hexyl 3,5,10, 100 400 17.5 4.00 1.5 - 10

10-PREP,15

CN 3,5,10 100 400 7.0 3.80 1.5 - 7.0

NH2

3,5,10 100 400 9.5 5.80 1.5 - 11



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5µ Microbore and MiniboreColumns (mm)

Phases 50 x 1.0 150 x 1.0 250 x 1.0 30 x 2.0 50 x 2.0 150 x 2.0 250 x 2.0 *4 x 2.0mm **4 x 3.0mm

Silica(2) — — — — 00B-4274-B0 00F-4274-B0 00G-4274-B0 AJ0-4347 AJ0-4348

C5 — — — 00A-4043-B0 00B-4043-B0 00F-4043-B0 — AJ0-4292 AJ0-4293

C8 — — — 00A-4040-B0 00B-4040-B0 00F-4040-B0 00G-4040-B0 AJ0-4289 AJ0-4290

C8 (2) 00B-4249-A0 00F-4249-A0 00G-4249-A0 00A-4249-B0 00B-4249-B0 00F-4249-B0 00G-4249-B0 AJ0-4289 AJ0-4290

C18 — — — 00A-4041-B0 00B-4041-B0 00F-4041-B0 00G-4041-B0 AJ0-4286 AJ0-4287

C18 (2) 00B-4252-A0 00F-4252-A0 00G-4252-A0 00A-4252-B0 00B-4252-B0 00F-4252-B0 00G-4252-B0 AJ0-4286 AJ0-4287

CN — — — 00A-4255-B0 00B-4255-B0 00F-4255-B0 00G-4255-B0 AJ0-4304 AJ0-4305

NH2 — 00F-4378-A0 00G-4378-A0 00A-4378-B0 00B-4378-B0 00F-4378-B0 00G-4378-B0 AJ0-4301 AJ0-4302

P he ny l-H ex yl 00 B- 425 7-A 0 00F -4 25 7-A 0 — 0 0A -4 257 -B 0 00 B-4 257 -B0 0 0F -4 25 7- B0 00 G- 425 7-B 0 A J0 -4 350 AJ 0- 43 51

SecurityGuard™Cartridges require universal holder Order No.: KJO-4282

ORDERING INFORMATION

*for column ID 2.0-3.0 -**for column ID 3.2-8.0

SecurityGuard

3µ AnalyticalColumns (mm)

Phases 30 x 3.0 50 x 3.0 150 x 3.0 30 x 4.6 50 x 4.6 75 x 4.6 100 x 4.6 150 x 4.6 *4 x 2.0mm **4 x 3.0mm

Silica(2) — — 00F-4162-Y0 — 00B-4162-E0 — 00D-4162-E0 00F-4162-E0 AJ0-4347 AJ0-4348

C8(2) 00A-4248-Y0 00B-4248-Y0 00F-4248-Y0 00A-4248-E0 00B-4248-E0 00C-4248-E0 00D-4248-E0 00F-4248-E0 AJ0-4289 AJ0-4290

C18 (2 ) 00A-4251-Y0 00B-4251-Y0 00F-4251-Y0 00A-4251-E0 00B-4251-E0 00C-4251-E0 00D-4251-E0 00F-4251-E0 AJ0-4286 AJ0-4287

C N — 0 0B -4 254 -Y 0 0 0F -4 254 -Y0 00A -4 25 4- E0 0 0B -4 254 -E0 00 C- 425 4-E 0 00 D- 42 54- E0 0 0F -4 254 -E 0 A J0- 430 4 A J0 -4 30 5

NH2 — 00B-4377-Y0 00F-4377-Y0 00A-4377-E0 00B-4377-E0 00C-4377-E0 00D-4377-E0 00F-4377-E0 AJ0-4301 AJ0-4302Pheny l-Hexy l — 00B-4256-Y0 00F-4256-Y0 00A-4256-E0 00B-4256-E0 00C-4256-E0 00D-4256-E0 00F-4256-E0 AJ0-4350 AJ0-4351


SecurityGuard

3µ Microbore and MiniboreColumns (mm)


Silica(2) — — — 00A-4162-B0 00B-4162-B0 00D-4162-B0 00F-4162-B0 AJ0-4347 AJ0-4348

C8(2) 00A-4248-A0 00B-4248-A0 00F-4248-A0 00A-4248-B0 00B-4248-B0 00D-4248-B0 00F-4248-B0 AJ0-4289 AJ0-4290

C18 (2 ) 00A-4251-A0 00B-4251-A0 00F-4251-A0 00A-4251-B0 00B-4251-B0 00D-4251-B0 00F-4251-B0 AJ0-4286 AJ0-4287

CN — — — 00A-4254-B0 00B-4254-B0 00D-4254-B0 00F-4254-B0 AJ0-4304 AJ0-4305

NH2

— — 00 F- 43 77- A0 00 A-4 377 -B 0 00B -4 377 -B0 0 0D -4 377 -B0 0 0F -43 77- B0 A J0- 43 01 A J0 -4 302

P he ny l-H ex yl 0 0A -4 256 -A 0 00B -4 25 6- A0 — 00 A-4 256 -B0 0 0B- 42 56- B0 00 D- 42 56 -B 0 0 0F -4 256 -B0 AJ 0- 43 50 A J0 -4 35 1


SecurityGuard

3µ and 5µ Capillary Columns (mm)

Phases 50 x 0.30 150 x 0.30 250 x 0.30 50 x 0.50 150 x 0.50 250 x 0.50 50 x 0.70 150 x 0.70 250 x 0.70

3µ C8(2) 00B-4248-AC 00F-4248-AC 00G-4248-AC 00B-4248-AF 00F-4248-AF 00G-4248-AF 00B-4248-AG 00F-4248-AG 00G-4248-AG




If Luna does not provide at leastan equivalent separation as com-pared to a competing column of thesimilar particle size, phase and

dimensions, send in your comparative data within 45 days and keep theLuna column for FREE.



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10µ Bulk Packings

Phase 100g 1kg 5kg 10kg 50kg 100kg

Silica(2) 04G-4091 04K-4091 04L-4091 04M-4091 04N-4091 04P-4091

Bonded Silica

C5 04G-4092 04K-4092 04L-4092 04M-4092 04N-4092 04P-4092

C8 04G-4093 04K-4093 04L-4093 04M-4093 04N-4093 04P-4093

C8(2) 04G-4250 04K-4250 04L-4250 04M-4250 04N-4250 04P-4250

C18 04G-4094 04K-4094 04L-4094 04M-4094 04N-4094 04P-4094

C18(2) 04G-4253 04K-4253 04L-4253 04M-4253 04N-4253 04P-4253

CN 04G-4300 04K-4300 04L-4300 04M-4300 04N-4300 04P-4300

NH2 04G-4379 04K-4379 04L-4379 04M-4379 04N -4379 04P-4379Phenyl-Hexyl

Phenyl-H exyl 04G-4285 04K-4285 04L-4285 04M-4285 04N-4285 04P-428510µ vs. 10µ- PREP :

What is the difference?

The Luna 10µ and 10µ-PREP differ by only

one factor: Particle size distribution. The

silica matrix and bonding chemistry are

identical, but the 10µ-PREP has a wider

particle size distribution to reduce cost.

Which should I choose?

Luna 10µ-PREP offers exceptional

performance, suitable for most appli-

cations, with excellent economy. Luna 10µ

is available when the absolute best prep-arative performance is critical. Extremely

narrow size distribution yields the highest

efficiency and maximum resolution.

Evaluation is the Best Test

All Luna materials are available in scout

columns. Contact your Phenomenex

representative to set up an evaluation.

10µor

10µ-PREP ?

10µ- PREP Bulk Packings


Silica(2) 04G-4322 04K-4322 04L-4322 04M-4322 04N-4322 04P-4322

Bonded Silica

C8(2) 04G-4323 04K-4323 04L-4323 04M-4323 04N-4323 04P-4323

C18(2) 04G-4324 04K-4324 04L-4324 04M-4324 04N-4324 04P-4324

Phenyl-Hexyl

Phenyl-H exyl 04G-4325 04K-4325 04L-4325 04M-4325 04N-4325 04P-4325

15µ Bulk Packings


Silica(2) 04G-4271 04K-4271 04L-4271 04M-4271 04N-4271 04P-4271

Bonded Silica

C8(2) 04G-4272 04K-4272 04L-4272 04M-4272 04N-4272 04P-4272

C18(2) 04G-4273 04K-4273 04L-4273 04M-4273 04N-4273 04P-4273

Phenyl-Hexyl

Phenyl-H exyl 04G-4286 04K-4286 04L-4286 04M-4286 04N-4286 04P-4286


15µ Preparative and Pilot ScaleColumns (mm)

Phases 250 x 4.6 250 x 21.2 250 x 30 250 x 50 250 x 100 50 x 21.2 50 x 50

Sil ica(2) 00G-4271-E0 00G-4271-P0 00G-4271-U0 00G-4271-V0 00G-4271-W0 03B-4271-P0 03B-4271-V0

C8(2) 00G-4272-E0 00G-4272-P0 00G-4272-U0 00G-4272-V0 00G-4272-W0 03B-4272-P0 03B-4272-V0

C18(2) 00G-4273-E0 00G-4273-P0 00G-4273-U0 00G-4273-V0 00G-4273-W0 03B-4273-P0 03B-4273-V0

Phenyl-Hexyl 00G-4286-E0 00G-4286-P0 00G-4286-U0 00G-4286-V0 00G-4286-W0 03B-4286-P0 03B-4286-V0

Guard(mm)

Phenomenex Tel. 310.212.0555 Fax 310.328.7768 Web www.phenomenex.co m


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Guard Cartridge System

Order No. Replacement Parts Unit Price

AJ0-4283 PEEK Ferrules 3/pk

AJ0-4285 Stacking Rings 2/pk

AQ0-1389 PEEK (Biocompatible) 10/pk Fingertight fittings

AJ0-4284 SecurityGuard™ Wrenches 2/pk

Method Development Kits

Order No. Description Dimensions(mm) Unit Price

K H0 -4 760 L un a M eth od D ev elo pme nt K it ( 5 co lu mn s) 1 50 x 4. 6 5 /p k 1 each of 5µ C18(2), C8(2), C5, CN, Phenyl-Hexyl Phases

KH0-4354 Luna Rapid-MD Kit (3 columns)1 eac h o f 3µ C18 (2 ), C8(2) a nd Pheny l-Hexy l pha ses 50 x 4 .6 3 /pk

KHO-4761 Luna Selectivity Kit 1 (3 columns)1 e ach o f 5 µ C 18( 2) , P he ny l- He xy l, CN ph as es 15 0 x 4 .6 3 /p k

KH0-4762 Luna Selectivity Kit 2 (3 columns)

1 e ac h o f 5 µ C 8( 2) , P he ny l- He xy l, CN p has es 15 0 x 4 .6 3 /p k

Method Validation Kits

Order No. Description Dimensions (mm) Unit Price

KH0-4355 Luna 5µ C18 Method Validation Kit 150 x 4.6 3/pk

KH0-4356 Luna 5µ C18(2) Me thod Validation Kit 150 x 4.6 3/pk

KH0-4357 Luna 5µ C8 Method Validation Kit 150 x 4.6 3/pk

KH0-4358 Luna 5µ C8(2) Method Validation Kit 150 x 4.6 3/pk

K H0 -4 359 L un a 5 µ P he ny l-H ex yl M eth od Val id at io n K it 1 50 x 4 .6 3 /p k

Holder Assembly Kit

Order No. Description Price

KJO-4282 Guard Cartridge Kit.Includes: 1 cartridgeholder, 3 PEEK ferrules,2 wrenches, 2 stackingrings, 2 PEEK fingertight fittings



Method

Development KitsThe Luna Method Development Kits

combine the incredible performance of

Luna HPLC columns with three different

selectivities. Both kits include a Luna

Phenyl-Hexyl (moderate polarity), a Luna

CN (polar), and your choice of a C8(2) or

C18(2) (non-polar). A wide range of

selectivies for virtually any separation

challenge.

Validation Kits

Three columns of the same phase from

three different batches provide an excellent

benchmark for system validation. Method

reprodicibility is easily and quickly

assessed, clearly demonstrating

chromatographic performance, validation

potential and long term compliance with

Luna Method Validation Kits.

CartridgesCartridges for General Purpose/Pharmaceutical

Order No. Material Dimensions Column ID pH Stability Unit PriceDescription (mm)

AJ0-4286 C18 4 x 2.0 2.0 - 3.0 mm 1.5 - 10 10/pk

AJ0-4287 C18 4 x 3.0 3.1 - 8.0 mm 1.5 - 10 10/pk AJ0-4289 C8 4 x 2.0 2.0 - 3.0 mm 1.5 - 10 10/pk

AJ0-4290 C8 4 x 3.0 3.1 - 8.0 mm 1.5 - 10 10/pk

AJ0-4292 C5 4 x 2.0 2.0 - 3.0 mm 1.5 - 10 10/pk

AJ0-4293 C5 4 x 3.0 3.1 - 8.0 mm 1.5 - 10 10/pk

AJ0-4347 Silica 4 x 2.0 2.0 - 3.0 mm — 10/pk

AJ0-4348 Silica 4 x 3.0 3.1 - 8.0 mm — 10/pk

AJ0-4350 Phenyl 4 x 2.0 2.0 - 3.0 mm 1.5 - 10 10/pk

AJ0-4351 Phenyl 4 x 3.0 3.1 - 8.0 mm 1.5 - 10 10/pk

AJ0-4304 CN 4 x 2.0 2.0 - 3.0 mm 1.5 - 7 10/pk

AJ0-4305 CN 4 x 3.0 3.1 - 8.0 mm 1.5 - 7 10/pk

AS0-4301 NH2

4 x 2.0 2.0 - 3.0mm 1.5 - 11 10/pk

AS0-4302 NH2

4 x 3.0 3.1 - 8.0mm 1.5 - 11 10/pk

Phenomenex Tel. 310.212.0555 Fax 310.328.7768 Web www.phenomene x.com


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5µ AnalyticalColumns (mm)


Silica(2) — — — — — 00B-4274-E0 — AJ0-4347 AJ0-4348

C5 — — 00F-4043-Y0 00G-4043-Y0 — 00B-4043-E0 — AJ0-4292 AJ0-4293

C8 — — 00F-4040-Y0 00G-4040-Y0 00A-4040-E0 00B-4040-E0 00C-4040-E0 AJ0-4289 AJ0-4290

C8(2) 00A-4249-Y0 00B-4249-Y0 00F-4249-Y0 00G-4249-Y0 00A-4249-E0 00B-4249-E0 00C-4249-E0 AJ0-4289 AJ0-4290C18 — — 00F-4041-Y0 00G-4041-Y0 0 0A-4041-E0 0 0B-4041-E0 0 0C-4041-E0 AJ0-4286 AJ0-4287

C18 (2 ) 00A-4252-Y0 00B-4252-Y0 00F-4252-Y0 00G-4252-Y0 00A-4252-E0 00B-4252-E0 00C-4252-E0 AJ0-4286 AJ0-4287

CN — 00B-4255-Y0 00F-4255-Y0 00G-4255-Y0 00A-4255-E0 00B-4255-E0 00C-4255-E0 AJ0-4304 AJ0-4305

NH2

— 00B-4378-Y0 00F-4378-Y0 00G-4378-Y0 00A-4378-E0 00B-4378-E0 00C-4378-E0 AJ0-4301 AJ0-4302

Phenyl-Hexyl 00A-4257-Y0 00B-4257-Y0 00F-4257-Y0 00G-4257-Y0 00A-4257-E0 00B-4257-E0 00C-4257-E0 AJ0-4350 AJ0-4351


5µ Analytical, Semi-Prep and PreparativeColumns (mm)

Phases 100 x 4.6 150 x 4.6 250 x 4.6 250 x 10 250 x 15 250 x 21.2 250 x 30 50 x 10 *4 x 2.0mm **4 x 3.0mm

Sil ica(2) 00D-4274-E0 00F-4274-E0 00G-4274-E0 00G-4274-N0 00G-4274-AK 00G-4274-P0 00G-4274-U0 03B-4274-N0 AJ0-4347 AJ0-4348

C5 00D-4043-E0 00F-4043-E0 00G-4043-E0 00G-4043-N0 — — — — AJ0-4292 AJ0-4293

C8 00D-4040-E0 00F-4040-E0 00G-4040-E0 00G-4040-N0 — — — 03B-4040-N0 AJ0-4289 AJ0-4290

C8(2) 00D-4249-E0 00F-4249-E0 00G-4249-E0 00G-4249-N0 00G-4249-AK 00G-4249-P0 00G-4249-U0 03B-4249-N0 AJ0-4289 AJ0-4290

C18 00D-4041-E0 00F-4041-E0 00G-4041-E0 00G-4041-N0 — — — 03B-4041-N0 AJ0-4286 AJ0-4287

C18 (2 ) 00D-4252-E0 00F-4252-E0 00G-4252-E0 00G-4252-N0 00G-4252-AK 00G-4252-P0 00G-4252-U0 03B-4252-N0 AJ0-4286 AJ0-4287

CN 00D-4255-E0 00F-4255-E0 00G-4255-E0 00G-4255-N0 — — — 03B-4255-N0 AJ0-4304 AJ0-4305

NH2

00D-4378-E0 00F-4378-E0 00G-4378-E0 00G-4378-N0 — –- — 03B-4378-N0 A J0-4301 A J0-4302

Phenyl-Hexyl 00D-4257-E0 00F-4257-E0 00G-4257-E0 00G-4257-N0 00G-4257-AK 00G-4257-P0 00G-4257-U0 03B-4257-N0 AJ0-4350 AJ0-4351


Guard (mm) SecurityGuardCartridges 10/pk

10µ Semi-Prep and PreparativeColumns (mm)

Phases 250 x 4.6 250 x 10 250 x 15 250 x 21.2 250 x 30 250 x 50 250 x 100 50 x 10 50 x 21.2 50 x 50 *4 x 2.0mm **4 x 3.0mm

Sil ica(2) 00G-4091-E0 00G-4091-N0 00G-4091-AK 00G-4091-P0 00G-4091-U0 00G-4091-V0 00G-4091-W0 03B-4091-N0 03B-4091-P0 03B-4091-V0 AJ0-4347 AJ0-4348

C5 00G-4092-E0 00G-4092-N0 — 00G-4092-P0 — 00G-4092-V0 00G-4092-W0 03B-4092-N0 03B-4092-P 0 03B-4092-V0 AJ0-4292 AJ0-4293

C8 00G-4093-E0 00G-4093-N0 — 00G-4093-P0 — 00G-4093-V0 00G-4093-W0 03B-4093-N0 03B-4093-P 0 03B-4093-V0 AJ0-4289 AJ0-4290

C8(2) 00G-4250-E0 00G-4250-N0 00G-4250-AK 00G-4250-P0 00G-4250-U0 00G-4250-V0 00G-4250-W0 03B-4250-N0 03B-4250-P0 03B-4250-V0 AJ0-4289 AJ0-4290

C18 00G-4094-E0 00G-4094-N0 — 00G-4094-P0 00G -4094-V0 00G-4094-W0 03B-4094-N0 03B-4094-P0 03B-4094-V0 AJ0-4286 A J0-4287

C18(2) 00G-4253-E0 00G-4253-N0 00G-4253-AK 00G-4253-P0 00G-4253-U0 00G-4253-V0 00G-4253-W0 03B-4253-N0 03B-4253-P0 03B-4253-V0 AJ0-4286 AJ0-4287

NH2

00G-4379-E0 00G-4379-N0 00G-4379-AK 00G-4379-P0 0 0G-4379-U0 0 0G-4379-V0 00G-4379-W0 — — — — —

Phenyl-Hexyl 00G-4285-E0 00G-4285-N0 00G-4285-AK 00G-4285-P0 00G-4285-U0 00G-4285-V0 00G-4285-W0 03B-4285-N0 03B-4285-P0 03B-4285-V0 AJ0-4350 AJ0-4351SecurityGuard™Cartridges require universal holder Order No.: KJ0-4282

SecurityGuardCartridges 10/pk

SecurityGuardCartridges 10/pk


5u Columns for High-Throughput PurificationColumns (mm)

Phases 50 x 21.2 100 x 21.2 150 x 21.2 50 x 30 75 x 30 100 x 30

Sil ica(2) 00B-4274-P0 00D-4274-P0 00F-4274-P0 00B-4274-U0 00C-4274-U0 00D-4274-U0

C8(2) 00B-4249-P0 00D-4249-P0 00F-4249-P0 00B-4249-U0 00C-4249-U0 00D-4249-U0

C18 (2 ) 00B-4252-P0 00D-4252-P0 00F-4252-P0 00B-4252-U0 00C-4252-U0 00D-4252-U0

*for column ID 2.0-3.0 -**for column I D 3.2 -8.0

Guard (mm)

Phenomenex Tel. 310.212.0555 Fax 310.328.7768 Web www.phenomene x.com

2320 W 205th St


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U.S.A. PUERTO RICO CANADA UNITED KINGDOM GERMANY NEW ZEALAND AUSTRALIA

TEL: (310) 212-0555 (800) 541-HPLC (800) 543-3681 01625-501367 06021-58830-0 09-4780951 1800 553 929

FAX: (310) 328-7768 (310) 328-7768 (310) 328-7768 01625-501796 06021-58830-11 09-4780952 1800 553 923

Luna® is available worldwide. For the distributor in your country contact Phenomenex USA,

International Department by telephone, fax or e-mail: [email protected]

®

The World’s #1 HPLC Column

2320 W. 205th St.

Torrance, CA 90501-1456

U.S.A.

P E B 2 0 1 8 1 I NT - 0

Documents

Hplc Column Phenomenex Luna