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L. tarentolaesuspension cperformed atarentolae w
All growth mLeishmania. completion wvitality the cexceeded, itweeks
For maintasuspension cculture (TC) ffaster. It is no
Best results successive doccasional hgrowth. It is incubate TCcultivate Leiscultivation foof the LEXSY
Always contrcultures are omotile. ThesMid-growth pof different scontaining sshape) and limitations afungal or oth
Keep patientwith regular They need th
If you - despsediment celincubation intransfection
Don’t centrifuby rigorous vat 2000-300pellets easier
If you cultivaaerate the cu109 cells/ml.
How
e needs aeculture with ret 26°C. Higill not survive
media should Hemin is l
with Hemin thcompleted mt is possible
aining LEXSYcultures in 10flasks. Don´t ot necessary
are obtainedilution of chigher dilutio
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manuals
rol appearanof drop-like sse cells arephase cultureshape. Don’tsuch subpopremain motnd must not
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t, esp. if youdoubling tim
heir time to g
pite followingls 3 min at 2n ventilated T
uge Leishmavortexing. Th00g is sufficier. If required,
ate LEXSY strulture in a 1. If you intend
Jena Bioscien
w to grow
erobic condegular dilutioher temperat
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be supplemeight-sensitivehe medium medium shoulto re-add H
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use agitatedor growth-pr
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pulations. Cetile. Enhance
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rains in bior0 L fermentad to use a sti
nce GmbH • LoebstedteTel.: +4
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ells by microin size with
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Jena • Germany • e-MFax: +49-3641-62851
ulture
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stirring. Weobtaining higforces
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ained as cols). All cultivaity significan
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venient to gr12) in ventilace cells will adium
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ontinuous ations are tly and L.
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row static ted tissue
age much
repeated However, ubsequent ay and to es. Don’t ution. For apter 5.4
wth phase end, and
d media. nd of cells p-like cells eedle-like or other
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ost strain, d continue esp. after
ell pellets rifugation on of cell
fficient to ties up to