Upload
gerard-sullivan
View
220
Download
0
Tags:
Embed Size (px)
Citation preview
How Do Mutations in DNA Affect the Function of Genes?
Mutations result from Nucleotide Substitutions, Insertions, or Deletions
Mutations may have a variety of effects on protein structure and function
Mutations provide the raw material for evolution
Chapter 10.
MUTATION Process of mutation may result in a gene
coding for a new protein Mutations are not good or bad, they do
provide the raw material of change. There are many different types of
mutations.
Chapter 10.
Nucleotide substitution can result in either a change or no change in amino acid of the codon.
MUTATION
Point Mutation - change in one nucleotide.
Insertion - new nucleotide pairs are inserted into DNA molecule
Deletion - nucleotide pairs are removed from DNA molecule
Chapter 10.
Different Types of Mutations
MUTATION Effect of Mutation on protein structure and function No change for protein gene codes for. No change in function of protein even though change
results in use of different amino acid (amino acids are functionally equivalent to each other).
Codes for a new amino acid that is functionally different from original.
Mutation codes for a stop codon, results in non-functional gene and protein.
Chapter 10.
- Change in a single nucleotide of a genetic sequence- Possible results:
- Base Substitution- Same Sense Mutation- Missense Mutation- Nonsense Mutation
- Frame Shift Mutation- Insertion- Deletion
Base Substitution
Same Sense Mutation
Mutations that result in replacement of one amino acid for another within a polypeptide chain. Mutant polypeptides still retain some residual biologic activity of the wildtype.
Missense Mutation
Nonsense Mutation
Nonsense mutations: Mutations that result in replacement of an amino acid codon with a termination codon production of an amino-terminal fragment of the normal polypeptide often result in complete loss of activity of the gene product.
Fitness effects of mutations
Some mutations are selectively neutral (i.e., they have no fitness effects), including:
•Mutations in non-coding DNA (i.e., regions not coding for protein product, transcription factor binding site, etc)
•Silent substitutions (i.e., mutations in coding regions that do not result in amino acid substitutions, because of redundancy in the genetic code)
Fitness effects of mutations, continued
Mutations that change amino acid sequences tend to be selectively non-neutral, including:
•Point mutations:
•Missense mutations: cause amino acid substitution
•Nonsense mutations: amino acid changed to stop codon (UAA, UAG, UGA), signalling termination of translation
Fitness effects of mutations, continued
Other mutations that change amino acid sequences include:
•Insertions and deletions (indels)
•Indels not in multiples of 3 nucleotides cause frameshift mutations
•Indels of 3 nucleotides, cause insertion/deletion of amino acid or nonsense mutation
Summary
Exposure to UV causes adjacent thymines to become dimer and disrupting their normal base pairing.
An enzyme cuts out and removes the damaged DNA.
DNA polymerase fits the gap by synthesizing new DNA, using the intact strand as a template.
DNA ligase seals the remaining gap by joining the old and bew DNA.
Non-ionizing Radiation Mutation
Mutation and Selection
Mutations are heritable changes in the genome. Spontaneous mutations in individual bacteria are rare. Some mutations cause changes in phenotypic characteristics. In microbial genetics, specific reference organisms are referred to as wild-type strains, and descendants having mutations in their genomes are called mutants. Variant forms of a specific genetic determinant are called alleles.
Genotypic symbols - lower case, italicized abbreviations that specify individual genes, with a (+) superscript indicating the wild type allele(eg. genotypic symbol for the ability to produce -galactosidase, required to ferment lactose, is lacZ+, and mutants are lacZ- )
Phenotypic symbols - capitalized and not italicized, to distinguish them from genotypic symbols. (Eg. The lactose-fermenting phenotype is designated LAC+, and inability to ferment lactose is LAC-)
Spontaneous and Induced Mutations
• The mutation rate in bacteria is determined by - accuracy of DNA replication - occurrence of damage to DNA - effectiveness of mechanisms for repair of damaged DNA.
• For a particular bacterial strain under defined growth conditions, the mutation rate for any specific gene is constant and is expressed as the probability of mutation per cell division. • the proportion of mutants usually increases progressively as the size of the bacterial population increases.
Reversion and Suppression
• Forward mutations - mutations that convert the phenotype from wild-type to mutant • Reverse mutations - mutations that change the phenotype from mutant back to wild-type, such bacteria are called revertants.
Reverse mutations that restore the exact nucleotide sequence of the wild-type DNA are true revertants – are identical to wild-type strains both genotypically and phenotypically.
Selection-After gene transfer -For strain carries gene of interest.- On specific media – replica plating technique, colonies patching
Detection of Mutant Phenotypes
Selective and differential media are helpful for isolating bacterial mutants.
Selective media - permit mutants to grow, but not wild-type strains.
Differential media - permit both wild-type & mutant bacteria to grow and form colonies that differ in appearance.
Identification of Mutant Cells
Positive (direct selection detects mutant cells because they grow or appear different.Negative (indirect) selection detects mutant cells because they do not grow.
Detection of Mutant PhenotypesConsider a wild-type strain of E coli that is susceptible to the antibiotic streptomycin
(genotype Strs) and can utilize lactose as the sole source of carbon (phenotype LAC+).
a) Selective media containing streptomycin: b) Selective media containing chemical analogs of lactose which can be converted
to toxic metabolites byproducts: c) Minimal media with lactose as the sole source of carbon: c) Differential media such as MacConkey-lactose agar or eosin-methylene blue-
lactose agar:
Detection of Mutant Phenotypes
Consider a wild-type strain of E coli that is susceptible to the antibiotic streptomycin (genotype Strs) and can utilize lactose as the sole source of carbon (phenotype LAC+).
a) Selective media containing streptomycin: Strr mutants grow, wild-type Strs bacteria are killed.
a) Selective media for LAC- mutants of E coli made by incorporating chemical analogs of lactose that are converted into toxic metabolites by LAC+ bacteria but not by LAC- mutants. Thus Lac- mutants grow, LAC+ wild-type bacteria are killed.
b) Minimal media with lactose as the sole source of carbon: LAC+ wild-type strains will grow, but LAC- mutants cannot grow.
c) Differential media such as MacConkey-lactose agar or eosin-methylene blue (EMB)-lactose agar: LAC+ wild-type and LAC- mutant strains of E coli can be distinguished by their color.
Conditional and suppressive mutations• Mutations that inactivate essential genes in haploid organisms
are usually lethal.
• Such potentially lethal mutations can often be studied if their expression is controlled by manipulation of experimental conditions.
• Temperature-sensitive and cold-sensitive mutations are examples of conditional mutations, as are suppressible mutations.
• A conditional lethal phenotype indicates that the mutant gene is essential for viability.
• Eg. a mutation that increases the thermo-lability of an essential gene product may prevent bacterial growth at 42°C, although the mutant bacterium can still grow at 25°C.
Conversely, cold-sensitive mutants express the mutant phenotype at low temperature, but not at high temperature.
Mutation creates new alleles.Natural selection affects the rate of fixation or loss of alleles in populations.
Mutation.
Purifying selection removes deleterious mutations.
Rare mutations with positive fitness consequences increase in frequency through natural selection--may eventually become fixed.Mutation continually introduces new allelesAll allele copies in a population share a common ancestor (the “coalescent”).
Extra-chromosomal Elements
Bacteriophages
bacterial viruses or phages Extrachromosomal Elements Can survive outside host cell Infect bacteria:
Replcate lysis of cell – lyticIntergrate without cell death = lysogenic
Infection of E. coli by Phage Virulent phage
replicate and kill their host by lysing or breaking it open
phage can infect cells but don’t necessarily kill
Two paths of reproduction Lytic mode: infection
progresses as in a virulent phage
Lysogenic mode: phage DNA is integrated into the host genome
Bacteriophages
Infectious agents, replicate as obligate intracellular parasites in bacteria Morphologically different - polyhedral, filamentous, and
complex (polyhedral heads with tails attached) consist of a protective shell (capsid) surrounding the
tightly packaged nucleic acid genome genomes vary in size (~ 2 to 200 kb); either dsDNA,
ssDNA, or RNA genes encodes proteins - for replication & phage
assembly
Lysis plaques of phage on E. coli bacteria lawn
Plasmid
First plasmid described was discovered in Japan in Shigella species during an outbreak of dysentery in the early 1940‘s
3 main components:
• Origin of replication• Selectable marker• Restriction enzyme site(s)
• Enzymes that cut at specific sequence on DNA
Plasmid – small, circular, extrachromosomal DNA which replicates independently of host chromosomal DNA
Plasmids Content
Replication factors
Genes
Ori Region
Ori, actual site of replication
Proteins that assist in replication (varies)
Recognition sequences for control factors
The ori determines the Range
The Large Virulence Plasmid of
Shigella flexneri
Plasmids
Discrete, extrachromosomal genetic elements in bacteria Usually much smaller than bacterial chromosome Size varies from < 5kb to > 100 kbp Mostly supercoiled, circular, ds DNA molecules Replicate independently of the chromosome Exist in multiple copies in bacterial (the average number of
plasmid per bacterial is called copy number). Usually encode traits that are non-essential for bacterial
viability.
Plasmid Genes
F plasmids
codes for sex factor of bacteria also called conjugative plasmids function: - genes promote transfer of plasmid - donor to recipient - genes code for proteins required for their replication usually large plasmids (>40 Kbp), small copy number (1 to
several per chromosome) partition themselves among daughter cells during cell
division similar to bacterial chromosome
R plasmids:
medically important, eg, resistant to Penicillin (carries genes of the Bla operon)
In early 1940's, Penicillin was introduced for general use 1946 - 14% of Staphylococcus aureus were penicillin resistant 1947 - 38% PenR 1969 - 59% PenR 1970's - almost 100% PenR
resistance to one or several antibiotics (R factor)
Col plasmids:
produce colicins, a type of bacteriocin that affect sensitive cells (Col-) & inhibit growth
may or may not be self-transmissible
ColE1 is mobilizable but non-conjugative size : <7.5 Kbp high copy numbers (typically 10-20 per chromosome) rely on their bacterial host to provide some functions
required for replication are distributed randomly between daughter cells at
division.
Many plasmids control medically important properties of pathogenic bacteria, contain genes that code for :
a) resistance to one or several antibiotics
b) production of toxins eg. heat-labile & heat-stable enterotoxins of E. coli, Shiga toxins of Shigella exfoliative toxin of S. aureus tetanus toxin of C. tetani c) synthesis of cell surface structures required for adherence or
colonization
Some plasmids are cryptic = no recognizable effects on the bacterial host
Comparing plasmid profiles = for assessing possible relatedness of individual clinical isolates of a particular bacterial species for epidemiological studies
Function of plasmids
Plasmid DNA replication
Plasmid replication by - Theta model (either uni- or bidirectional) or - Rolling circle Replicon - DNA molecules that can replicate autonomously (plasmids, chromosomes, phage) Replicon must have on origin of replication, called ori Functions of the ori region: Host range - narrow or broad host ranges Broad-host-range plasmids = encode all of their own proteins
required for replication initiation Regulation of copy number Stringent - low copy number (F factor) Relaxed - high copy number (pBR322 =16 copies; pUC =30 to 50) Requires host proteins for replication
Theta Model
Replication fork
Ori Rep
Replication fork
Ori Rep
Replication bubble
Rolling circle replication
Enable rapid synthesis of multiple copies of circular DNA or RNA (plasmid or phage genomes).
A striking feature: = one strand is
replicated first (which protrudes after being displaced) and the second strand is replicated after completion of the first one.
Mechanism of Rolling circle DNA replication
An initiator protein encoded by the plasmid DNA nicks one strand of the ds plasmid at the ori site.
The initiator protein binds to the 5' PO4 end of the nicked strand The free 3' OH end serve as a primer for DNA synthesis by DNA
polymerase III, using the un-nicked strand as a template. The 5' PO4 ssDNA strand is displaced by helicase PcrA in the presence of
the initiation protein. Continued DNA synthesis can produce multiple ss linear copies of the
original DNA in a continuous head-to-tail series called a concatemer. These linear copies are converted to ds circular plasmid by: the initiator protein makes another nick to terminate synthesis of the first (leading) strand. DNA polymerase III replicate the ss ori to make complementary strand, RNA primer removed, DNA ligase joins the
ends to make ds circular plasmid.
Plasmid amplification and curing
Plasmid amplification by chloramphenicol treatment
- inhibits protein synthesis - inhibit chromosomal but not plasmid replication. - Chromosomal replication requires new protein synthesis but plasmid replication uses only stable bacterial replication
proteins. - Plasmids replicated to high copy number because no repressor protein to control copy number
Plasmid curing - with acridine orange - inhibits plasmid but not chromosomal replication - unknown how this occurs
Plasmid is an ideal structure for genetic engineering because Simple in structure
Easy to extract & isolate in the lab
Easy for genetic manipulation & transformed back into bacteria
Contains genetic information which can be used by the bacteria
Most plasmid present in high copy number
Plasmid codes for antibiotic resistant gene eg. Ampicillin, Ap r or Tetracyclin Tcr - selection of bacteria with transformed plasmid.
Non-essential for bacteria’s growth, thus possible to manipulate plasmid
DNA without affecting bacteria growth.
Exchange of Genetic Information in bacteria
Medically important - rapid emergence and dissemination of antibiotic resistance plasmids - flagellar phase variation (eg. Salmonella) - antigenic variation of surface antigens (eg. Neisseria & Borrelia)
Sexual processes in bacteria involve transfer of genetic information from a donor to a recipient, results in:
- substitution of donor alleles for recipient alleles - addition of donor genetic elements to the recipient genome.
3 major types of genetic transfer found in bacteria: a) Transformation b) Transduction c) Conjugation In all three cases, recombination between donor and recipient DNA result in
formation of stable recombinant genomes
Types of transfers:
Non-transmissible = cannot initiate contact with recipient or transfer DNAConjugative = can initiate contact with recipient bacteriumMobilizable = can prepare its DNA for transferSelf-transmissible = is both conjugative & mobilizable
4 stages of plasmid transfer: a) Effective contact
b) Mobilization - preparation for DNA transfer c) DNA transfer d) Formation of F in recipient
Donation - a conjugative plasmid (F) can provide conjugative function to a mobilizable plasmid (eg. ColE1) such that both plasmids can be transferred.
Plasmid conduction - a self-transmissible plasmid (F) can recombine with a non-mobilizable plasmid and transfer the co-integrate.
Fin genes & plasmid transfer
fin genes - fertility inhibition
- codes for repressor that prevents transcription of genes
required for transfer.
F plasmid has 1 fin gene so transfer system is always ‘ON’
R plasmid has 2 fin genes so cannot always transfer. - in new recipients (repressor is absent) so transfer
can occur soon after receiving the R plasmid but after time (when repressor is made) transfer can't occur
introducing DNA from donor to recipient result in uptake and integration of fragments of donor DNA
into recipient genome. produce stable hybrid progeny. is most likely to occur when the donor and recipient bacteria
the same or closely related species.
(a) Bacterial Transformation
"Transformation" is simply the process where bacteria manage to "uptake" a piece of external DNA. Usually, this process is used in the laboratory to introduce a small piece of PLASMID DNA into a bacterial cell.
Bacteria transformation in the lab
Bacteriophage infect donor bacterium form rare abnormal bacteriophage particles contain DNA from
donor bacteria. abnormal bacteriophage infect recipient bacteria & inject DNA into
recipient donor DNA integrated / recombined into recipient DNA resulting in
transduced bacterium.
Bacteria Transduction
Bacterial Conjugation
Transfer of DNA between 2 bacteria in contact with each other
Contact between donor and recipient (initiated by sex pili)
DNA transfer through a conjugation bridge Mediated by a plasmid
Called an F-factor (fertility factor) or conjugative plasmid
Several important properties of F
F is a self-replicating plasmid and is maintained in a dividing cellular population.
Cells carrying F produce pili, minute proteinaceous tubules that allow the F+ cells to attach to other cells maintaining contact.
F+ cells can transfer its F plasmid to a F- cell , turning the recipient cell into an F+ cell. F+ cells are usually inhibited from making contact with each other.
Occasionally, F can integrate into the host bacterial chromosome and transfer the host chromosomal markers to the recipient cell.
consist of methylases = methylate the adenine or cytosine residues at specific sequences in their own DNA
corresponding restriction endonucleases cleave foreign DNA which are not methylated at the same target sequences.
RM systems = protect bacteria against invasion by phages or plasmids.
Barrier to genetic exchanges between different bacterial strains or species.
Recent evidence suggests that plasmid-borne RM systems = strategy to ensure plasmid maintenance in a host strain since cells that
lose these plasmid (and the corresponding protective methylase gene) are killed by restriction enzyme, which attacks the newly replicated & unmodified chromosomal DNA.
Restriction-modification (RM) systems