Homework: Complete Step 4 (Summary) on Intro to Enzymes worksheet Silent Do Now:

Homework: Complete Step 4 (Summary) on Intro to

Enzymes worksheet Silent Do Now:

Silently pass your typed McMush Lab Report forward

(on the Intro to Enzymes handout) What comes to mind when you hear the word enzyme? What do you think an enzyme is?

Today’s Objectives: Describe what an enzyme does and how it

works Explain why an enzyme’s shape is so

important

Bio 9A/9D: Tuesday, 11.2.10Title: Introduction to Enzymes!

Web-Based Activity:Guided Notes on Enzymes

Go to “LewPort enzymes” (first link on google)

http://www.lew-port.com/10712041113402793/lib/10712041113402793/Animations/Enzyme_activity.html

Read aloud the blue text on each slide Use this info to fill in the Notes column on

the back of your worksheet Then use the animations on the Enzyme

Menu to fill in drawings or descriptions in the Evidence column

We will do the first one together… You will work quietly w/ your assigned

partner to complete the rest.Objectives for Class:

Describe what an enzyme does and how it works Explain why an enzyme’s shape is so important



Kenny

Emiliano

Yamile

Lennin

Martin

Amrita

Javier

Lorena

Lila

Imani

Nathy

Jesse

Hajar

BryanVictor

Martely

Daysia

Devin

Naidaliz

Joshua

Joelle

Cassandra

BeverlyErick

OliviaAlerte

Web-Based Activity:Guided Notes on Enzymes

Go to “LewPort enzymes” (first link on google)


Read aloud the blue text on each slide Use this info to fill in the Notes column on

the back of your worksheet Then use the animations on the Enzyme

Menu to fill in drawings or descriptions in the Evidence column

We will do the first one together… You will work quietly w/ your assigned

partner to complete the rest.Objectives for Class:

Describe what an enzyme does and how it works Explain why an enzyme’s shape is so important



Homework: Now: Take out Intro to Enzyme worksheet Tonight: Starch Digestion Pre-Lab Questions (pages 2-

3 #1-6)Silent Do Now: (in NB w/ title & date)Describe or define each of the following terms.

What does each one mean to you? If you’re not sure, write what you think. What questions do you have about them? How do these terms relate to each other? Chemical reaction Enzyme Reactant Product

Bio 9A/9D: Wednesday, 11.3.10Title: ENZYMES

You should be writing silently for the entire Do Now time.

Today’s Objectives & Agenda

Objectives: Describe and illustrate chemical reactions,

reactants, products, and enzymes Explain how enzymes work to catalyze

reactions Use a knowledge of enzymes to predict what

will happen in tomorrow’s lab Agenda:

Notes: Vocabulary & Example Pre-Lab for tomorrow’s lab Start HW

Objectives for Class: Describe and illustrate chemical reactions, reactants, products, and enzymes Explain how enzymes work to catalyze reactions Use a knowledge of enzymes to predict what will happen in tomorrow’s lab

Chemical Reaction:Process that changes or transforms one substance into another

Either to build something from smaller pieces – Synthesis

Or to break something down – DecompositionEx: glucose + O2 CO2 + H2O + ATP

energy + heat Enzyme:

A protein that speeds up chemical reactions in living things (the “catalysts” in our cells and bodies)

Chemical Reaction Vocabulary:

Chemical Reaction Vocabulary continued:

Reactants:the starting materials in a chemical reaction(also known as substrates)

Products:substances that are produced in the chemical reaction (the end result)


The enzyme lipase helps to break down lipids into their building block molecules (fatty acids and glycerol)

Enzyme – Reactant (aka Substrate) – Products – Lipids Fatty acids +

glycerolSubstrate/ Reactant Products

LipaseEnzyme helps

substrate turn into products

Example: Lipid Digestion


Lipase Lipi

dsFatty acids and glycerol

Which enzyme is best structured to help break down lipids? Why?


1 32Objectives for Class:

Describe and illustrate chemical reactions, reactants, products, and enzymes Explain how enzymes work to catalyze reactions Use a knowledge of enzymes to predict what will happen in tomorrow’s lab


2

Which enzyme is best structured to help break down lipids? Why?

The SHAPE of an enzyme must match the SHAPE of its specific substrates!Objectives for Class:

Describe and illustrate chemical reactions, reactants, products, and enzymes Explain how enzymes work to catalyze reactions Use a knowledge of enzymes to predict what will happen in tomorrow’s lab

Enzyme Lab Preview:Starch Digestion

Background Info… Procedure

Summary… Work w/ partner

to answer pre-lab questions #1-6

HW: Finish the pre-lab questions!

Starch & Water

Starch & Water & Enzyme(salivary amylase)


Homework:Enzyme Lab Analysis Questions (typed or neatly

written on separate paper) Silent Do Now: (in notebook w/ title & date)

1. In 1-2 sentences, explain what this chemical equation means:

STARCH GLUCOSE2. Identify the reactant, product, and enzyme

in this reaction.3. Which two substances will we add to the

test tube at the beginning of our experiment?4. Which substance will be in the test tube at the

end of the experiment that wasn’t there at the beginning?

Bio 9A/9D: Thursday, 11.4.10Title: Enzyme Lab: Starch Digestion

salivary amylase

Today’s Objectives & Agenda Today’s Objectives:

Identify the reactant, product, and enzyme in a chemical reaction

Predict how an enzyme affects the speed of a chemical reaction

Use proper lab procedures to test how saliva enzymes affect the speed at which starch breaks down into glucose

Today’s Agenda: Review Do Now & Lab Procedures Do the lab!

1 2

Enzyme Lab: Starch Digestion STARCH GLUCOSE

Procedure

Prediction:

Results: How will we know?

Starch & Water

Starch & Water & Enzyme(salivary amylase)

Which tube will contain glucose after 10 minutes? Fill in the Prediction row on your lab table right now.

salivary amylase

*Stir the starch well before using it!

Benedict’s test

Enzyme Lab: Starch Digestion STARCH GLUCOSE

Safety: Goggles on! Be careful around hot plates! Stay at your own lab station, do not talk across

the room No eating/drinking

Clean Up: Pour test tubes into waste container Rinse test tubes in sink Put tray containing all materials on side counter Turn off hot plates if everyone at your table is

done If finished early: discuss Analysis Question #2

with your partner

salivary amylase

Kenny

Emiliano

Yamile

Lennin

Martin

Amrita

Javier

Lorena

Lila

Emiliano

Nathy

Jesse

Hajar

BryanVictor

Martely

Daysia

Devin

Naidaliz

Joshua

Joelle

Cassandra

BeverlyErick

OliviaAlerte

Kenny

Yamile

LenninMartin

Amrita

Javier

Lorena

Lila

EmilianoNathy

Jesse

Hajar

Bryan

Victor

Martely

Daysia

Devin

Naidaliz

Joshua

Joelle

Cassandra

Beverly

Erick Olivia

9A – New Seats You are sitting with your partner from the Enzyme Lab

Homework:Type up the Background Info and Purpose &

Hypothesis sections of your Denaturing Enzymes lab report (see rubric for details)

Silent Do Now:Complete questions 1 and 2 on the Denaturing Enzymes packet.

Today’s Objectives: Define and illustrate denaturing and explain how it

affects the function of enzymes Develop a specific experimental question and

hypothesis related to denaturing enzymes

Bio 9A: Monday, 11.8.10Title: Enzyme Lab Extension

Quick Review: Enzyme-Catalyzed Reactions

Objectives for Class: Define and illustrate denaturing and explain how it affects the function of enzymes Develop a specific experimental question and hypothesis related to denaturing enzymes

Enzyme = Protein that “Speeds Up” a Chemical Reaction

Enzymes are a “catalyst”

Products = Stuff Produced from a Chemical Reaction “Enzyme – Substrate

Complex”

Substrate = Reactants of a Chemical Reaction

“Active Site” – where the substrate bonds to the enzyme

This shows the reaction happening!!!

Denaturing EnzymesWhen an enzyme is denatured, it… Becomes misshapen and deformed Loses its original shape Becomes unable to speed up the

reaction it is designed for, because its shape no longer matches its substrate

Design Your Own Experiment

Work with your partner to complete pages 2 and 3 of the Denaturing Enzymes Lab Packet Purpose and Hypothesis Background Information

If you finish early, move on to the Procedure section (page 4)

HW: Write up the Background Info and Purpose & Hypothesis sections of the lab report See rubric for details Type if possible (and bring electronic copy

on Wed.)Hand in Analysis Questions at the end

of today’s class

Background Info Brainstorm Enzyme Denature Salivary amylase Heat or pH (acid/base) (depending on

your question) Glucose Substrates Products Chemical reaction Starch

Homework:Write (type if possible) the Procedure section of

your Denaturing Enzymes lab report (see rubric for details)

Silent Do Now: (in NB w/ title and date) Summarize what it means for an enzyme to be

denatured. Is this generally a good thing or a bad thing? Why?

Describe the experiment you are planning. How will you denature the enzyme? What do you think will happen?

Today’s Objectives: Write a clear, specific, and replicable procedure for carrying

out an experiment relating to denaturing enzymes Design a clear data table for recording the results of an

experiment

Bio 9A: Wednesday, 11.10.10Title: Denaturing Enzymes Lab (Day 2)

Revised Work Policy You must turn in the original assignment

and rubric, along with your revisions Posters: Redo OR just add missing info Lab Reports: Type revisions and reprint Staple them all together

Revisions due FRIDAY if you want them to show up on your 1st quarter report card

Otherwise, they still count towards raising your 1st quarter grade but won’t show up until the next report card

Denaturing Enzymes Lab:Procedure & Data Table

Work with your partner to complete pages 4 and 5 of the Denaturing Enzymes Lab Packet Plan your Procedure by answering the questions Get Dickson’s approval Write the Steps and Materials for your

procedure Write Materials List on clipboard on kidney

On page 6: Create your data table HW: Write up the Procedure section of the

lab report See rubric for details Type if possible (and bring electronic copy on

Friday) Friday: Do the experiment, then laptops for

typing!

Kenny

Imani

Yamile

Lennin

Martin

Amrita

JavierLorena

Lila

Emiliano

Nathy

Jesse

Hajar

BryanVictor

Martely

Daysia

Devin

Naidaliz

JoshuaJoelle

Cassandra

Beverly

Erick

Olivia Alerte

Homework:Finish typing the Lab Report (all sections) for the

Denaturing Enzymes Lab Silent Do Now:

Take out the Denaturing Enzymes Lab packet On page 6, create a data table for recording the

results of your experiment. Remember to include: Space to record results from all of your test tubes Space to record observations as well as +/- results

Bio 9A: Friday, 11.12.10Title: Denaturing Enzymes Lab (Day 3)


Design a clear, specific, and replicable procedure and a clear data table for your experiment

Carry out your experiment Write a formal lab report

Agenda:1. Procedure Checklist (do this silently while Dickson

sets up groups)2. Get final procedure approved3. Gather materials4. Carry out experiment5. Discuss results w/ Ms. Dickson6. Type Lab Report (sit at front desks)

Kenny

YamileLennin

Martin Amrita

JavierLorena Lila

Emiliano

Nathy

Jesse

HajarBryan

Victor Martely

Daysia

Devin

Naidaliz Joshua

Joelle

Cassandra

Beverly

Erick Olivia

9A – New Seats for today

Alerte

Imani

Homework:Revise your lab report based on today’s peer

editing. Final draft due tomorrow, with rubric! Silent Do Now:

Take out your lab report On the top, write:

1. What is one thing you think you have done well in this rough draft?

2. What is one thing you think you could improve? What would you most like feedback/suggestions on?

Bio 9A: Monday, 11.15.10Title: Denaturing Enzymes Lab Wrap-Up


Compare and contrast experimental results between different groups, and draw class-wide conclusions

Use the lab report rubric and peer editing sheet to give and receive helpful feedback on the Denaturing Enzymes lab report

Agenda:1. Groups report out on results2. Peer editing of lab reports

In your notebook set up a place for Denaturing Enzyme Lab Class Results How does HEAT affect enzymes?

How does extreme pH affect enzymes?

Class Results:How does HEAT affect enzymes?

Group No enzyme Room Temp (25oC)

Boiling Water (100oc)

Joshua, Javier, Erick

+ Slight + / Slight -

Yamile, Hajar, Olivia

- + +

Lila, Devin

- + Slight +/-

Daysia, Victor

- + Slight +

Cassandra &

Nathy

- + -

+ = positive result (glucose was present, reaction occurred)– = negative result (no glucose present, reaction didn’t occur)

In your notebook set up a place for Denaturing Enzyme Lab Class Results How does HEAT affect enzymes?

Boiling enzymes denatures some enzymes but not all

Maybe needed to boil them longer Heat doesn’t make enzymes completely

useless, just less effective How does extreme pH affect enzymes?

Lemon juice (acid) and bleach (base) denature enzymes

Hydrochloric acid is not consistent but using more works better

Maybe household items work better than pure acids?

Class Results:How does pH affect enzymes?+ = positive result (glucose was present, reaction occurred)– = negative result (no glucose present, reaction didn’t occur)

Group No enzyme

Low pH (ACID)

Neutral pH High pH (BASE)

Martely, Emiliano

Slight + Lemon juice:

Complete -

+

Jesse, Martin

Slight + + Bleach:Complete -

Joelle, Lorena

Slight + HCl:-

+

Lennin, Bryan

Slight + HCl:Slight +

+

Amrita,

Kenny

Slight + 2 mL: +4 mL:

slight +

+


Compare and contrast experimental results between different groups, and draw class-wide conclusions

Use the lab report rubric and peer editing sheet to give and receive helpful feedback on the Denaturing Enzymes lab report

Agenda:1. Groups report out on results2. Peer editing of lab reports – this is silent

individual work time1. Switch lab reports with your assigned partner.2. Read the lab report and evaluate each section

(just circle boxes, don’t give points/grades)3. Fill out the feedback form on the back of the

rubric.4. Last 5 min. of class = time for quiet partner

discussion

Homework:Study for tomorrow’s quiz Silent Do Now: (in NB w/ title and date)

Take out your lab report and rubric and put it on the corner of your desk

Take out your assignment sheet and find the study guide on the back.

Answer questions 1 and 2 in your notebook (refer to notes from 10/20/10 if you can’t remember)

Bio 9A: Tuesday, 11.16.10Title: Quiz Review: Carbon Compounds & Enzymes

Quiz Review: Carbon Compounds & EnzymesToday’s Objectives & Agenda Today’s Objectives:

Review important information for tomorrow’s quiz Identify specific topics that require additional

review Agenda:Work individually or with a partner to quietly

complete the study guide If you want to work with a partner, raise your

hand and ask. Write answers to all study guide questions in

your notebook. If noise levels get too loud… 3rd warning = silent

individual work Raise your hand if you need to move across the room Work silently or talk quietly with your partner only

Homework:Read Section 38.2 and complete the reading

guide Do Now:

Clear your desks and get quiet for the quiz When Finished with the Quiz:

Get a textbook and start the HW Today’s Objectives:

Demonstrate what you know about carbon compounds and enzymes on the quiz

Bio 9A: Wednesday, 11.17.10Title: Quiz: Carbon Compounds & Enzymes

Documents

Homework: Complete Step 4 (Summary) on Intro to Enzymes worksheet Silent Do Now: