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High Throughput Plant Phenotyping at the National Plant Phenomics Centre Dr. Marie Neal et al. 15th May 2014

High Throughput Plant Phenotyping at the National Plant ... · LemnaTec platform for the systematic large scale, high throughput phenotyping of diversity and mapping populations in

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Page 1: High Throughput Plant Phenotyping at the National Plant ... · LemnaTec platform for the systematic large scale, high throughput phenotyping of diversity and mapping populations in

High Throughput Plant Phenotyping at

the National Plant Phenomics Centre

Dr. Marie Neal et al.

15th May 2014

Page 2: High Throughput Plant Phenotyping at the National Plant ... · LemnaTec platform for the systematic large scale, high throughput phenotyping of diversity and mapping populations in

Talk Outline

About the NPPC

Where we are

What facilities we have

Implications of high-throughput phenotyping

Examples of Our Work

How you can use our facilities and work with us

Page 3: High Throughput Plant Phenotyping at the National Plant ... · LemnaTec platform for the systematic large scale, high throughput phenotyping of diversity and mapping populations in

Our Facility

Page 4: High Throughput Plant Phenotyping at the National Plant ... · LemnaTec platform for the systematic large scale, high throughput phenotyping of diversity and mapping populations in

Our Facility

LemnaTec platform for the systematic large scale, high throughput phenotyping

of diversity and mapping populations in crops and model species. 3 conveyor

connected glasshouse compartments with 850 RFID carriages

Page 5: High Throughput Plant Phenotyping at the National Plant ... · LemnaTec platform for the systematic large scale, high throughput phenotyping of diversity and mapping populations in

Our Facility

5 Imaging chambers:

RGB (biomass)

Root NIR and VIS and Laser

Thermal IR (stomata function)

NIR (water content)

Page 6: High Throughput Plant Phenotyping at the National Plant ... · LemnaTec platform for the systematic large scale, high throughput phenotyping of diversity and mapping populations in

Our Facility

Temperature controlled Glasshouse and heat

map produced from 21 distributed sensors.

Page 7: High Throughput Plant Phenotyping at the National Plant ... · LemnaTec platform for the systematic large scale, high throughput phenotyping of diversity and mapping populations in

Our Facility

Watering station, and graph illustrating water regulation

Also nutrient and spraying cabins

Page 8: High Throughput Plant Phenotyping at the National Plant ... · LemnaTec platform for the systematic large scale, high throughput phenotyping of diversity and mapping populations in

Phenotyping at different

scales at IBERS

Nutrient flow (hydroponics) UAV field phenotyping e.g. Forest health

Molecular analysis - HiSeq, Mass spec, FTIR/NIR,

Micro Raman, Marvin Grain Analyser

Photo-physiology – PAMFluorometer, chlorophyll

Imager, Gas analyser

Page 9: High Throughput Plant Phenotyping at the National Plant ... · LemnaTec platform for the systematic large scale, high throughput phenotyping of diversity and mapping populations in

High throughput Phenotyping

Very large amounts of data

We can't look at it all

Aim to provide a set of Automated techniques, moving from

Manual to computer assisted to fully automated analysis

Application pipeline in development

Tell us things we can't measure manually

Page 10: High Throughput Plant Phenotyping at the National Plant ... · LemnaTec platform for the systematic large scale, high throughput phenotyping of diversity and mapping populations in

Images from the chambers

Root RGB Blue light photo-chemical reactions

Multi plant top view RGB – Model plants and crops for

biomass, rate of growth over time 3D depth maps of grass

Page 11: High Throughput Plant Phenotyping at the National Plant ... · LemnaTec platform for the systematic large scale, high throughput phenotyping of diversity and mapping populations in

Experiments

Page 12: High Throughput Plant Phenotyping at the National Plant ... · LemnaTec platform for the systematic large scale, high throughput phenotyping of diversity and mapping populations in

Experiments

Page 13: High Throughput Plant Phenotyping at the National Plant ... · LemnaTec platform for the systematic large scale, high throughput phenotyping of diversity and mapping populations in

Experiments

Page 14: High Throughput Plant Phenotyping at the National Plant ... · LemnaTec platform for the systematic large scale, high throughput phenotyping of diversity and mapping populations in

Access via Website

Page 15: High Throughput Plant Phenotyping at the National Plant ... · LemnaTec platform for the systematic large scale, high throughput phenotyping of diversity and mapping populations in

Access via Website

Page 16: High Throughput Plant Phenotyping at the National Plant ... · LemnaTec platform for the systematic large scale, high throughput phenotyping of diversity and mapping populations in

Summary

About the NPPC – our smarthouse and other

Phenotyping facilities

Our High-throughput phenotyping data -

Images and environmental data gives us

Very Large amounts of data which we can't look at

manually

Illustrated some example experiments

How you can use our facilities and work with us

Page 17: High Throughput Plant Phenotyping at the National Plant ... · LemnaTec platform for the systematic large scale, high throughput phenotyping of diversity and mapping populations in

NPPC team

Contact: Marie Neal

[email protected]