High Incidence of Lipid Deposition in the Liver of Rats Fed a Diet Supplemented with Branched-Chain Amino Acids under Vitamin B6 Deficiency

Tae Kaimoto, Mayumi Shibuya, Kazutaka Nishikawa and Hideo MaedaDepartment of Living ScienceShikoku University, Tokushima, Tokushima 779-1192 Japan

Elizabeth Katzman2.27.14

What are Branched Chain Amino Acids (BCAA)?

http://agron-www.agron.iastate.edu/Courses/Agron317/AA_inhibitors.htm

How Does this Fit with B6

Roles of BCAA

Suppresses fatigue in CNS.

Prevention of Hepatic Encephalopathy.

Recovery of burn victims.

Required for protein synthesis.

Contribute to energy metabolism.

Problems with Supplementation

Lack of information

High protein diets with B6 deficiency

Biochemical parameters

Fatty liver

Previous Study

Accumulation of

triacylglycerol in the liver

B6 Deficiency

BCAAs

20% Casein

Hypothesis

“It is possible that a disorder of amino acid metabolism in the presence of vitamin B6 deficiency might influence the assembly of lipids and apolipoproteins and/or the secretion of lipoproteins.”

What are Apolipoproteins?

https://www.mabtech.com/knowledge-center/applied-research/apolipoproteins/research-studies-apolipoproteins

Diets and Animals

20% vitamin-free casein diet 21 days Sacrificed

Isolation of Lipoproteins and Purification of Apo E

• Pooled serum of male Wistar Rats

VLDL

• Preparative Ultracentrifugation

APO E

• Column Chromatography

Purification of Apo E

Column Chromatography

http://www.waters.com/waters/en_US/HPLC---High-Performance-Liquid-Chromatography/nav.htm?cid=10048919&locale=en_US

Spectrophotometer

Serum and Hepatic Apolipoproteins

http://virology-online.com/general/Test1.htm

Enzyme-Linked Immunosorbent Assay (ELISA)

Enzyme-Linked Immunosorbent Assay (ELISA)

http://medical-dictionary.thefreedictionary.com/enzyme-linked+immunosorbent+assay

Lipoprotein Analysis

High Performance Liquid Chromatography (HPLC)

Growth Parameters

Serum and Hepatic Lipids

Serum and Hepatic Apolipoprotein Levels

HPLC Analysis of Serum

Conclusion

“Therefore, it can be inferred from our data that the secretion of synthesized lipoproteins from the liver to the bloodstream might be impaired.”

What does it all mean?

Triacylglycerol and APO E are getting stuck in the liver Fatty Liver Disease

Supplementation can do more harm than good.