Biochemical Systematics and Ecology 29 (2001) 10251047
Herbivory, induced resistance, and interplantsignal transfer in Alnus glutinosa
Teja Tscharntkea,*, Sabine Thiessena,b, Rainer Dolcha,Wilhelm Bolandb
aAgroecology, University of G .ottingen, Waldweg 26, D-37073 G .ottingen, GermanybMax-Planck Institute for Chemical Ecology, Carl-Zeiss-Promenade 10, D-07745 Jena, Germany
Received 9 April 2001; accepted 19 April 2001
Field experiments with manually defoliated black alders (Alnus glutinosa) showed that
defoliation aected herbivory by the major alder antagonist, the leaf beetle Agelastica alni.Herbivore damage increased with increasing distance to the defoliated tree, suggesting inducedresistance not only on the damaged tree, but also on the neighbouring trees. The beetles alsoavoided leaves from the nearest neighbours for both feeding and oviposition in a laboratory
assay, so the alders showed interplant resistance transfer. Natural enemies did not appear toshape this pattern, because the number of entomophagous arthropods and predatorpreyratios even increased with increasing distance to the defoliated tree. The numbers of all
specialist, but not the generalist, herbivore species paralleled the increase in the attack of thespecialist A. alni, supporting the view that specialists are more aected by plant resistance thangeneralists.
Mechanisms causing this pattern, found in the eld, were studied in more detail usingbiochemical analyses and further bioassays. Responses of alder leaves to herbivory of A. alniwere shown to include ethylene emission and the release of a blend of volatiles with mono-,
sesqui- and homoterpenes. Changes in leaf chemistry after herbivory included increases in theactivity of oxidative enzymes (polyphenoloxidase, PPO, lipoxygenase, LOX, and peroxidase,POD) and proteinase inhibitors (PIs), and an increase in the phenolic contents of the leaves.Quantication of the endogenous jasmonic acid (JA) showed the activation of the
octadecanoid pathway following herbivory.The active components in mediating a possible interplant signal transfer via airborne
volatiles may have included ethylene, b-ocimene, 4,8-dimethylnona-1,3,7-triene (DMNT), and4,8,12-trimethyltrideca-1,3,7,11-tetraene (TMTT). The incubation with volatiles resulted in an
*Corresponding author. Tel.: +49-551-399209; fax: +49-551-398806.
E-mail address: firstname.lastname@example.org (T. Tscharntke).
0305-1978/01/$ - see front matter r 2001 Elsevier Science Ltd. All rights reserved.
PII: S 0 3 0 5 - 1 9 7 8 ( 0 1 ) 0 0 0 4 8 - 5
increase in the activity of catalase (CAT) and PIs (after MeJA application) and in an increase
in the content of phenolics and PI activity (after ethylene application). Further evidence thatairborne interplant communication may be important in the response of alder trees to beetleattack came from container experiments. In airtight chambers, unattacked leaves signicantlyincreased the activity of proteinase inhibitors when they were associated with leaves previously
attacked by beetle larvae.In conclusion, eld experiments, bioassays in the laboratory as well as biochemical analyses
suggest the existence of interplant resistance transfer in A. glutinosa, with airborne volatiles as
a possible mechanism. However, the relative importance of airborne and possible soil-bornesignals as well as unknown eects of intensied nutrient absorption of defoliated trees,possibly reducing foliage quality of undamaged neighbours, remains to be shown. r 2001
Elsevier Science Ltd. All rights reserved.
Keywords: Talking trees; Leaf beetles; Volatiles; Proteinase inhibitors; Jasmonic acid; Phenolics; Ethylene
Plant responses to herbivory are known from a wide range of plants and oftenaect subsequent herbivory (e.g. Karban and Baldwin, 1997; Agrawal et al., 1999).Plant responses to insect herbivory may not only concern the damaged plantsthemselves but also undamaged neighbours, making them less susceptible toherbivores. Such interplant signal transfer has been hypothesized for a long time (e.g.Baldwin and Schultz, 1983; Rhoades, 1983). Communication among plantsremained a hot topic in the public, although Fowler and Lawton (1985), whosework did not support a talking tree hypothesis, reviewed the published studiesdealing with interplant signal transfer and criticized most of them because ofunsuitable experimental design or statistical aws such as pseudoreplication. Despitetheir harsh criticism, discussion has been refuelled as recent work has yielded resultsin favour of interplant communication (e.g. Bruin et al., 1995; Shonle and Bergelson,1995; Karban et al., 2000; Arimura et al., 2000). Attack of herbivores on one plantmay aect the non-attacked, neighbouring plant via connection by roots ormycorrhiza (Simard et al., 1997) or via volatiles such as methyl salicylate (MeSA)and methyl jasmonate (MeJA) (Farmer and Ryan, 1990; Shulaev et al., 1997; Bolandet al., 1998; Thaler et al., 1996; Karban et al., 2000). Two recent papers give evidencethat interplant communication may be important under natural eld conditions, inboth herbs (Karban et al., 2000) and trees (Dolch and Tscharntke, 2000).Field observations suggested the existence of resistance transfer among alders
(Alnus glutinosa) (Dolch and Tscharntke, 2000). After manual defoliation, leafdamage of alder leaf beetle (Agelastica alni, Col. Chrysomelidae) increased withdistance to the defoliated tree. This eect may be attributed to interplantcommunication. Why have black alders been expected to show signal transferamong plants? Black alders were chosen, because they are known to becomeregularly and completely defoliated by their specic, widespread, univoltine and
T. Tscharntke et al. / Biochemical Systematics and Ecology 29 (2001) 102510471026
most important enemy, the alder leaf beetle, and such a periodic and strong selectionpressure may be associated with induced and strong defense responses. In addition tothe conspicuous ushcrash uctuations in this system (Tscharntke and Dolch, pers.obs.), previous work had shown that herbivory caused both rapid and delayedinduced resistance in alders (Jeker, 1981; Baur et al., 1991; Seldal et al., 1994;Oleksyn et al., 1998; Tscharntke and Dolch, unpubl. data).Additional experiments in the eld were conducted to search for further evidence
for the pattern found by Dolch and Tscharntke (2000), and to further elaborate theinterplant-communication hypothesis. Since eects of defoliation on neighbouringtrees may not only aect performance of the alder leaf beetle, we also studied theresponse of the total arthropod community associated with alder leaves. In addition,the volatile compounds emitted by attacked leaves and the induced changes in leafchemistry were analysed in the laboratory. We show in this paper that damagedalder plants produce biologically active volatiles, which are possible candidatesubstances for the interplant resistance transfer observed in the eld. Bioassays withunattacked leaves, neighbouring leaves attacked by beetles as well as the applicationof airborne signals such as ethylene, MeJA and MeSA revealed plant physiologicalresponses to volatile cues.
2. Material and methods
2.1. Field experiments
We selected 10 sites in the vicinity of G .ottingen (Germany) with 10 trees of blackalder growing in rows along a creek, so we studied altogether 100 alders (for detailssee Dolch and Tscharntke, 2000). At each site, one randomly selected tree waschosen for defoliation. Manual defoliation took place in early May 1994 (beforeadult beetles had colonized alders) and included stripping 20% of the trees leavesfrom the lower branches of the canopy. Mean distance between each of the 10 treeswas 1m. Leaf damage was estimated as percentage of total leaf area consumed on all100 trees at six dates between May and September. In the laboratory, ovipositionbehaviour was tested using equally aged leaves from each site, and, in a furtherexperiment, beetles could choose between these leaves for consumption.In addition to the experiments with alder leaf beetles, we took samples of all
arthropods on the alder leaves from all defoliated trees, their nearest neighbours andthe farthest trees. The three lowest branches of each of the altogether 30 trees werestrongly knocked three times on four dates between May and September (before and37, 81 and 133 days after defoliation), and the arthropods dropped in an umbrellathat was held beneath these branches. Sampling the insect fauna on Alnus glutinosayielded phytophagous insects, grouped in specialists (monophagous and/or knownto prefer Alnus) and non-specialists (according to Schwenke, 1972, 1974), andentomophagous arthropods.
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2.2. Biochemical analyses and bioassays
For the laboratory experiments, six-months old black alder trees Alnus glutinosa(nursery Grebenstein & Linke, Germany) were grown for 12 months in a plasticpot (+=10 cm) with a mixture of potting soil and sand (1 : 1, v/v) in walk-in growthchambers with 241C and a 16:8 h day : night regime. These 18-months old trees(height=4060 cm, stem +=0.7 cm) were assigned for treatments so that eachtreatment group had plants of similar size and appearance. Agelastica alni (L.) larvaewere reared from eggs of adults sampled in the eld. The larvae were fed with freshlycut A. glutinosa foliage and reared at 201C with a 16:8 h day : night regime.
2.3. Container experiments
The container experiments should show possible eects of volatiles produced byherbivore-damaged alder leaves on undamaged leaves. We placed one branch withthree leaves from intact alder plants in a clip cage to prevent the larvae fromescaping. Five larvae of A. alni were placed on each leaf and allowed to feed for 72 h.In the rst desiccator (2700ml), the A. alni-infested leaves (=A. alni) were kepttogether with one branch with three healthy leaves of black alder (=A-neighbour). We analysed the black alder physiological responses (PI activity) inthe leaves damaged by A. alni and in the leaves exposed to the volatiles. The seconddesiccator had the same container set-up, but without feeding of A. alni: one branchwith three leaves from a healthy plant (=control) and another healthy branch asneighbour of control leaves (=C-neighbour) were kept together. Assays weremaintained at room temperature (r.t.) with a 16:8 h day : night regime and 4000 lx.Experiments were repeated six times and for each repetition and treatment, dierentdesiccators were used.
2.4. Application of ethylene, jasmonic acid methyl ester (=MeJA), salicylic acidmethyl ester (=MeSA), and jasmonic acid (=JA), and insect feeding
For induction experiments with volatiles (ethylene, MeJA, MeSA), we detachedthree fresh leaves from intact black alder plants, transferred them into a vial (5ml)with tap water, and enclosed the leaves in a desiccator (2700ml). The chemicals,except ethylene, were dissolved in pure dichloromethane (1 mg ml1), and 5 ml of eachwas applied onto a small piece of lter paper. After brief evaporation of the solvent,we xed the lter paper as a dispenser below the cap of the desiccator to preventany direct contact between the chemical and the leaves. Control experiments werecarried out following the same procedure but without the application of the testcompounds onto the lter paper. Ethylene was applied using 1000 ml of the pure gas.Jasmonic acid was applied as a solution (1mM) in tap water. For insect feeding,three leaves of black alder were kept together with 15 larvae of A. alni. The leaveswere exposed to the airborne substances, JA, and insect feeding for 48 or 72 h. Theseexperiments were repeated ve times (for the determination of activities of PPO,LOX, POD, CAT, and PI, see below) or six times (for the phenolics), using dierent
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desiccators for each treatment or replicate. The experimental set-up was maintainedat 251C and with a photophase of 16 h and 4000 lx.
2.5. Collection and analyses of alder volatiles
For the volatile induction experiments, stems of young A. glutinosa plants withthree developed primary leaves were cut and immediately transferred into glass vialscontaining a solution of the test substance in tap water. Feed-induced volatiles wereobtained by allowing 10 larvae of A. alni to feed on the alder leaves. The cut plantletswere enclosed in glass desiccators (2700ml). The experimental set-up was maintainedat 251C and with a photophase of 16 h.After pre-incubation of 24 h, the emitted volatiles were continuously collected over
a period of 24 h on small carbon traps (1.5mg charcoal, CLSA-Filter, Le Ruisseaude Montbrun, F-09350 Daumazan sur Arize) with air circulation according toDonath and Boland (1995). Compounds were eluted from the carbon traps withdichloromethane (2 15 ml) and 1 ml aliquots were injected into a 2201C injector andseparated by capillary GC on a fused silica-column (15m 0.25mm i.d.) coated witha 0.1 mm medium polar stationary phase (Optima-5s MS, Machery and Nagel,D .uren, Germany). Compounds were eluted under programmed conditions: 401C for1min, ramped at 101Cmin1 to 1801C, followed by a 351Cmin1 ramp to 2801C for3min. The He carrier gas was maintained at a ow rate of 3.0mlmin1. Elutingcompounds were detected by mass spectrometry (Finnigan GCQ) with a sourcetemperature of 1801C operated in EI (70 eV) mode. GC-interface at 2651C; solventdelay: 2min; scan range 35300Da. Compounds (caryophyllene, 4,8-dimethylnona-1,3,7-triene (=DMNT), 3-hexenyl acetate, indole, linalool, methyl salicylate, b-ocimene, 4,8,12-trimethyltrideca-1,3,7,11-tetraene (=TMTT)) were identied byretention time and mass spectra of authentic standards.Ethylene production was measured in real time with a photoacoustic laser
spectrometer containing a line-tunable infrared CO2 laser and a resonantphotoacoustic cell. Stems of young A. glutinosa plants with three developed primaryleaves were cut and immediately transferred into glass vials containing tap water.These plants were placed in a 600ml glass cuvette which received a constant ow of1 l h1 air. One cuvette contained the leaves with continuous feeding of A. alni (veadults on each leaf), while the second one contained only leaves (without beetles) andserved as reference cuvette. Ambient air was drawn through a platinum catalyst at4501C prior to entering the cuvette to remove hydrocarbons. After the cuvette, theair was pulled through a liquid-nitrogen trap to remove CO2 and H2O beforeentering the photoacoustic detection cell in which ethylene concentrations weremeasured every 3min for 36 h. The detection limit of this method was about 100 pptand calibration was performed with certied ethylene samples.
2.6. Quantication of endogenous jasmonic acid (=JA) and salicylic acid (=SA)
For measuring the endogenous levels of JA and SA, we detached three fresh leavesfrom intact black alder plants, transferred the leaves into a vial (5ml) with tap water,
T. Tscharntke et al. / Biochemical Systematics and Ecology 29 (2001) 10251047 1029
and enclosed the three leaves in a desiccator (2700ml). For feeding experiments velarvae of A. alni were placed on each leaf and allowed to feed continuously for thewhole time of incubation (t=0, 1, 3, 5.5, 8, 10, 12 h). Control experiments werecarried out following the same procedure but without insect feeding. Theseexperiments of a time course were repeated four times. Assays were maintained atr.t. with a 16:8 h day : night regime and 4000 lx.The quantication of endogenous JA and SA of the leaves (1 g FW) followed the
protocol of Koch et al. (1999) adapted from the original procedure of McCloud andBaldwin (1997) and was calculated using calibration curves of the standards.
2.7. Quantication of total content of phenolic compounds
For determination of phenolic compounds, the black alder leaves were driedovernight at 501C. The dried leaves (0.5 g) were ground for 3min with a Ultra-turraxT25 in 15ml of methanol. Then the homogenate was heated upto 751C for 3 h. Aftercooling, the extract was centrifuged at 2500g for 3min and the supernatant was usedfor the following measurement. A modication of the FolinCiocalteau method wasused for measurement of total content of phenolic compounds (Martin and Martin,1982). A 10 ml extract was delivered to an Eppendorf tube and mixed with 840 mlwater. The diluted extract was mixed with 50 ml Folin reagent (FolinCiocalteausphenol reagent, Sigma) and the mixture was allowed to incubate for 25min. Then100 ml of Na2CO3 (saturated) was added. After a 30min incubation at r.t. theabsorbance was measured at 750 nm on a Perkin-Elmer Spectrophotometer Lambda2S. Four replicates of each sample were analysed. The standard curve was preparedwith known concentrations of guaiacol.
2.8. Plant enzyme assays
To assay for foliar enzymes, the foliage, with midribs removed, was ash frozen inliquid nitrogen and homogenized in 0.1M ice-cold NaPO4-buer, pH 7.2, containing0.1% (w/v) sodium dodecyl sulphate (SDS) and 0.1% (w/v) polyvinylpyrrolidone(PVPP). The homogenate was centrifuged at 41C for 10min at 12,000g, thesupernatant was frozen in liquid nitrogen, and stored at 801C until used forspectrophotometric assays of PPO, LOX, POD, and CAT activities. Protein contentof the samples was determined in duplicate using the Bradford (1976) method, andthe activities of all four enzymes were expressed as DEmin1mg1 protein.To determine the polyphenol oxidase (PPO) activity, the procedure of Sherman
et al. (1991) was followed. The assay solution consisted of 850 ml l-3,4dihydroxyphenylalanine (DOPA (5mgml1) in 0.1M NaPO4-buer, pH 7.2, whichhad been aerated for 5min prior to assay. To eliminate interfering H2O2 andperoxidase activities, catalase (280Unitsml1, Sigma) in 100 ml H2O was added. Theassay was started by addition of 50 ml of enzyme extract. Assays were carried out foreither 2 or 10min depending on the amount of PPO activity present in the samplesand the PPO activity was estimated spectrophotometrically at 490 nm and r.t., tofollow the conversion of l-3,4 DOPA to quinone polymers.
T. Tscharntke et al. / Biochemical Systematics and Ecology 29 (2001) 102510471030
To determine the lipoxygenase (LOX) activity, the formation of UV-activeconjugated diene hydroperoxides was measured at 234 nm (Hildebrand andHymowitz, 1981). The reaction mixture consisted of 50 ml of enzyme extract addedto 950 ml of 1mM linoleic acid dispersed in a 0.1M NaPO4-buer (pH 7.0). Changein absorbance was monitored for at least 5min.To determine peroxidase (POD) activity, guaiacol was used as the hydrogen donor
according to the procedure of Ridge and Osborne (1970). Fifty microlitres of enzymesolution was mixed with a substrate solution consisting of 10mM guaiacol in 0.1MNaPO4-buer (pH 7.0) with H2O2 added as a cofactor. POD activity was measuredas increase for 5min at 470 nm.Catalase (CAT) activity was assessed by monitoring the decrease in H2O2 by
measuring the absorbance at 240 nm (Faccioli, 1979). The substrate was 30mMH2O2 in 0.1M NaPO4
-buer (pH 7.0)
2.9. Proteinase inhibitor (=PI) extraction and quantitative determination of trypsinproteinase inhibitor (=Tryp PI) activity using a radial diusion assay
The leaf samples for the PI analyses were weighed in a tube (1 g FW), ash frozenin liquid nitrogen, ground with a glass pestle, and thawed on ice. Before weighing,the midribs of the leaves were removed. After addition of 1ml ice-cold proteinextraction buer (1ml g1 leaf tissue; 0.1mM Tris HCl-buer (pH 7.6), containing5% polyvinylpolypyrrolidine, 2mgml1 phenylthiourea, and 5mgml1 diethyl-dithiocarbamate, 0.05M Na2EDTA), the samples were vortexed and centrifuged at41C for 20min at 12,000g. The supernatant was transferred to a fresh Eppendorftube and kept on ice until protein and PI analysis.Protein content of the samples was determined in duplicate using the Bradford
method (Bradford, 1976). PI activities were analysed with the radial diusion assayaccording to Jongsma et al. (1993, 1994), using bovine trypsine (type III; Fluka, 1mgml1) dissolved in agar. The leaf extract was allowed to incubate for 16 h at 41C inthe agar. Following incubation a 25ml of N-acetyl-phenylalanine-b-naphtylester(APNE, a trypsin substrate, Bachem) in DMSO-/Tris buer (0.1mM, pH 7.6)-mixture was added to the trypsin/PI-extract mixture for 1 h at 371C.For Tryp PI activities, a series of soybean Tryp inhibitor (STI, Boehringer
Mannheim, Mannheim, Germany) solutions were used to obtain a reference curve.PI activities are expressed as mg PImg1 protein g1 FW.
As the chemical assays used in this study are destructive, chemical analyses of theleaves could not be done both before and after the treatment, so dierences fromcontrol plants were established. Data analysis has been done using the softwareSTATISTICA 5.0 (StatSoft). Dierent treatments were compared using one-wayANOVA (Figs. 1 and 57), the StudentNewmanKeuls multiple range test, andt-tests (Fig. 8). In the gures, we give arithmetic means + one standard error.
T. Tscharntke et al. / Biochemical Systematics and Ecology 29 (2001) 10251047 1031
3.1. Field experiments
Leaf damage of the alder trees increased with distance to the manually defoliatedtree (Dolch and Tscharntke, 2000). Distance and leaf damage were best correlated 7days after defoliation, but this correlation held up to 3781 days. The feeding andoviposition experiments in the laboratory supported these ndings from the eld, asmost eggs were laid on leaves from the most distant alder tree, whereas leaves of thedefoliated tree and its nearest neighbour were avoided. Similarly, beetles (in the lab)consumed more area of the leaves from the farthest tree than from the defoliated treeor its nearest neighbour (Dolch and Tscharntke, 2000).The response of the total arthropod community to the manual defoliation was
very similar to that of the alder leaf beetle. Before defoliation, abundance and speciesrichness did not dier, but 81 days after defoliation, abundance of all arthropods(F 10:4; n 30; po0:001), of the phytophagous species (F 3:8; po0:05) as wellas the entomophagous species (F 9:0; po0:001) increased with distance to thedefoliated tree. This response was stronger than that after 37 or 133 days. Specialistsand non-specialists among the herbivores reacted dierently (Figs. 1A and B). Whilethe non-specialists did not show a signicant pattern, the specialists exhibited a cleardierence between the trees. The altogether 94 species of phytophagous insectssampled from A. glutinosa comprised 25 specialized (monophagous and/or known toprefer Alnus) and 69 non-specialized species. These species were represented by atotal of 1286 individuals, 980 (76.3%) of which belonged to the 20 most abundantspecies shown in Table 1. The altogether 75 species of entomophagous arthropods
Fig. 1. Number of individuals of phytophagous arthropods on the defoliated trees, their nearest
neighbours and their farthest neighbours (0, 1.3 and 10.6m distance to the manually defoliated tree), 81
days after defoliation (see Table 1). Arithmetic means + one standard error are shown, dierent letters
indicate signicant dierences: (a) phytophagous non-specialists: F 0:44; n 30; p 0:65; (b)phytophagous specialists: F 6:43; n 30; po0:005:
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consisted of 361 individuals (including 187 individuals and 28 species of spiders, 110individuals and nine species of true bugs, and 33 individuals and 26 species ofparasitoids). Table 1 presents 265 individuals (73.4%) of the entomophagousarthropods belonging to the 10 most abundant species. In addition, the predatorprey ratio (abundance of predators divided by abundance of prey, 81 days after thedefoliation) increased from the defoliated tree to its nearest neighbour and the 10mdistant tree (F 2:8; n 30; p 0:08).
Number of arthropod individuals collected on Alnus glutinosa, 81 days after defoliation (other than
Species No. of individuals
Specialist phytophagous insects
Psylla alni (Homoptera, Psyllidae) 67
Phyllonorycter alnifoliella (Lepidoptera, Gracillariidae) 29
Pterocallis alni (Homoptera, Callaphididae) 15
Alnetoidia alneti (Homoptera, Cicadellidae) 7
Ennomos alniaria (Lepidoptera, Geometridae) 7
Aphrophora alni (Homoptera, Cercopidae) 5
Oncopsis alni (Homoptera, Cicadellidae) 2
Melasoma aenea (Coleoptera, Chrysomelidae) 2
Psallus ambiguus (Heteroptera, Lygaeidae) 2
Chalcoides aurata (Coleoptera, Chrysomelidae) 1
Non-specialist phytophagous insects
Corticarina gibbosa (Coleoptera, Lathridiidae) 38
Baeopalma foersteri (Homoptera, Psyllidae) 25
Apion avipes (Coleoptera, Apionidae) 10
Apion nigritarse (Coleoptera, Apionidae) 10
Eupteryx aurata (Homoptera, Cicadellidae) 8
Stenocranus minutus (Homoptera, Delphacidae) 5
Semiothisa alternaria (Lepidoptera, Geometridae) 4
Trioza urticae (Homoptera, Psyllidae) 3
Dicyphus epilobii (Heteroptera, Miridae) 2
Eupteryx atropunctata (Homoptera, Cicadellidae) 2
Deraeocoris lutescens (Heteroptera, Miridae) 32
Singa hamata (Araneida, Araneidae) 19
Araneidae gen. sp. (Araneida) 15
Blepharidopterus angulatus (Heteroptera, Lygaeidae) 14
Tetragnatha sp. (Araneida, Tetragnathidae) 12
Araniella sp. (Araneida, Araneidae) 12
Forcula sp. (Dermaptera) 11
Metellina sp. (Araneida, Metidae) 9
Linyphiidae gen. sp. (Araneida) 6
Orius vicinus (Heteroptera, Anthocoridae) 4
aThe 10 most abundant species of both Alnus specialists and non-specialists, as well as the 10 most
abundant entomophagous species are shown.
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3.2. Biochemical analyses and bioassays
3.2.1. Volatile induction experimentsIn response to herbivory by A. alni, alder leaves released a blend of volatiles that
comprised the following compounds (Fig. 2): monoterpenes (e.g. b-ocimene,linalool), sesquiterpenes (e.g. b-caryophyllene, b-farnesene, a-humulene), homo-terpenes (e.g. 4,8-dimethylnona-1,3,7-triene, 4,8,12-trimethyltrideca-1,3,7,11-tetra-ene), fatty acid derivatives (e.g. 3-hexenyl acetate, decanal), and aromaticcompounds such as 2-methyl anthranilate, methyl salicylate, and indole. Herbivoryby A. alni induced a massive emission of these compounds that were not emitted by
Fig. 2. Gaschromatographic prole of the alder volatiles emitted after feeding by Agelastica alni. In
response to herbivory, black alder leaves released a blend of volatiles comprising the following
compounds: monoterpenes (e.g. b-ocimene, linalool), sesquiterpenes (e.g. b-caryophyllene, b-farnesene,a-humulene) homoterpenes (e.g. 4,8-dimethylnona-1,3,7-triene, 4,8,12-trimethyltrideca-1,3,7,11-tetraene),fatty acid derivatives (e.g. 3-hexenyl acetate, decanal) and aromatic compounds such as 2-methyl
anthranilate, methyl salicylate and indole. Control: gaschromatographic prole of undamaged alder
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healthy leaves. Although the collection of headspace volatiles from plants in closedsystems may lead to deprivation of CO2 and, hence may impose an additionalstress onto the plant, as yet, no negative impact has been observed. Moreover,headspace volatiles from control plants were collected the same way exposing thisgroup of plants to identical constraints. Volatile production was exclusively observedafter herbivore damage (or jasmonic acid treatment, Thiessen, unpublished)demonstrating that the experimental conditions do not signicantly alter the plantsresponse.
3.2.2. Ethylene emissionThe temporal pattern of ethylene emission from alder leaves over a 36 h period is
illustrated in Fig. 3. The rst volatile signs of A. alni feeding were observed 8 h afterstarting the experiment. The increase in emission was relatively long-lived (slowbuild-up within about the rst 10 h and waning within 24 h) in response to feeding.The ethylene production from leaf beetle-damaged tissue was not only greater than
Fig. 3. Ethylene emission (nl/l) from Alnus glutinosa plants during feeding of Agelastica alni adults,
measured by photoacoustic laser spectroscopy: (a) feeding of A. alni. Data measurement for the ethylene
emission due to the beetles feeding was shortly interrupted (as indicated by the double line); (b) control
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that from the control tissue, but showed a peak of emitted ethylene (7 nl l1) at 16 h,whereas control tissue showed a relatively steady rate of emission (1.4 nl l1) over themeasurement time.
3.2.3. Quantication of endogenous JA and SAThe enhanced levels of endogenous JA and SA showed the activation of the
octadecanoid and the salicylate pathway (Fig. 4). Samples were taken at dened timeintervals after allowing the herbivore to feed on the leaves. The level of endogenousJA rose 2 h after the onset of the experiment, reached a maximum of ca. 1255 ng g1
FW after 8 h, and then fell back within 12 h to the initial concentration of about13 ng g1 FW. Control experiments without feeding larvae resulted in only a smallincrease of endogenous JA, and the maximum level was much lower (ca. 81 ng g1
FW, due to the wounding by cutting the stem) (Fig. 4A).Temporal patterns of endogenous SA are shown in Fig. 4B. In the case of
herbivore damage, the level of endogenous SA at rst decreased and then steadilyincreased to 2000 ng g1 FW from hour 3 to 8. Later, free SA dropped to a nal levelof 1295 ng g1 FW, ca. 30% above the resting level prior to damage. Control leavesshowed a much lower increase and a lower maximum level of SA than the herbivore-damaged leaves. After a transient decrease, after the onset of the experiment, thelevel of endogenous SA reached a nal concentration at about 1121 ng g1 FW.
3.2.4. Quantication of total phenolic compoundsThe phenolic content of the alder leaves depended on the type of treatment
(Fig. 5). Control plants contained only 5.370.3mg phenolics g1 leaf DW. Feedingof larvae induced a signicant increase to 8.570.8mg g1. Treatment with gaseousethylene resulted in a similar increase of phenolics (10.271.1mg g1), but incubationwith gaseous MeJA and MeSA did not signicantly aect the level of phenolics.
Fig. 4. Jasmonic acid (JA) and salicylic acid (SA) concentrations of black alder leaves wounded at time 0
with feeding of Agelastica alni larvae, and leaves without herbivores (=control). Leaves from four alder
plants were harvested at each time: (a) JA concentration is expressed as ng JA per g leaf FW and; (b) SA
concentration is expressed as ng SA per g leaf FW. Arithmetic means 7 one standard error is shown(n 4). (&) Control; (K) feeding of A. alni. FW= fresh weight.
T. Tscharntke et al. / Biochemical Systematics and Ecology 29 (2001) 102510471036
Fig. 5. Phenolic content of black alder leaves treated with larvae of A. alni or with gaseous chemicals:
ethylene, jasmonic acid methyl ester (MeJA), and salicylic acid methyl ester (MeSA). Phenolic content (mg
phenolic per g leaf DW) was measured after 72 h of wounding or incubation with ethylene, MeJA and
MeSA. Arithmetic means + one standard error is given (n 6). Dierent letters indicate signicantdierences (po0:05). DW=dry weight.
Fig. 6. Enzyme activities in black alder leaves, after 48 h feeding of A. alni larvae or incubation with
gaseous chemicals: ethylene, jasmonic acid methyl ester (MeJA), or salicylic acid methyl ester (MeSA).
Arithmetic means + one standard error are given (n 5). Dierent letters indicate signicant dierences(po0:05): (a) polyphenol oxidase (PPO). (DE490min1mg1protein); (b) lipoxygenase (LOX)(DE234min
1mg1protein); (c) peroxygenase (POD) (DE470min1mg1protein); (d) catalase (CAT)
T. Tscharntke et al. / Biochemical Systematics and Ecology 29 (2001) 10251047 1037
3.2.5. Plant enzyme assaysAlder leaves were exposed to feeding of A. alni and incubated with gaseous
ethylene, MeJA and MeSA. After an induction period of 48 h, leaf PPO, LOX, PODand CAT activity was measured (Fig. 6). After feeding of A. alni PPO activitiesincreased 3.2-fold compared to the control. The feeding also increased the activitiesof LOX 5.7-fold and that of POD 13.6-fold. The activity of the antioxidant enzymeCAT showed a non-signicant increase (1.5-fold). In contrast, treatment of alderleaves with the volatiles ethylene, MeJA and MeSA did not enhance the activities ofPPO, LOX and POD, and only application of MeJA resulted in a 2.2-fold increase inCAT activity.
3.2.6. Proteinase inhibitor activityThe eects of feeding of A. alni, elicitation by JA solution, gaseous ethylene,
MeJA, or MeSA on the activity of PI were determined by the radial diusion assay.The data (Fig. 7) clearly indicate that alder leaves generally exhibited a low PIactivity (controls: 3.970.5 mg PImg1 protein g1 FW) that is signicantly enhancedby herbivory (4.7-fold), and the gaseous phytohormone ethylene (3.5-fold), withintermediate levels in the JA treatment (2.5-fold) and airborne MeJA (3-fold).
3.2.7. Container experimentsUndamaged leaves exposed to volatiles released from leaves damaged by
herbivory showed a signicant, 1.9-fold increase of PI activity compared to control
Fig. 7. Proteinase inhibitor (PI) activity in black alder leaves, monitored by radial diusion assay after
72 h of feeding of A. alni larvae or incubation with jasmonic acid (JA), ethylene, jasmonic acid methyl ester
(MeJA), or salicylic acid methyl ester (MeSA). Activity is expressed as mg PI mg1 protein g1 FW.Arithmetic means + one standard error are given (n 5). Dierent letters indicate signicant dierences(po0:05). FW=fresh weight.
T. Tscharntke et al. / Biochemical Systematics and Ecology 29 (2001) 102510471038
leaves (p 0:0065; Fig. 8), whereas combinations of undamaged leaves withundamaged leaves did not show any signicant changes in PI activity.
The results of the eld experiments with manually defoliated alders showed thatdefoliation aected herbivory by the major alder antagonist, the leaf beetle A. alni.Herbivore damage increased with increasing distance to the defoliated tree,suggesting induced resistance not only on the damaged tree, but also in theneighbouring trees. The beetles also avoided leaves from the nearest neighbours forboth feeding and oviposition in a laboratory assay (Dolch and Tscharntke, 2000).This pattern suggests that alders show interplant resistance transfer (inducedresistance in alders has been already shown by Jeker (1981) and Oleksyn et al.(1998)), possibly due to an airborne signal transfer with volatiles. Emission ofvolatiles to induce resistance may be advantageous for plants because of (i) a within-plant induction of resistance, as aerial dispersion of volatiles may lead to a fastertransfer to other tissues of the same tree than a systemic signal transfer within thevascular system (see Jones et al., 1993, and references therein), (ii) masking ofdefoliated trees by non-nutritious neighbours, similar to eects of associatedresistance of plants grown in polyculture rather than in monoculture (see Strong
Fig. 8. Proteinase inhibitor (PI) activity of undamaged alder leaves inuenced by volatiles emitted by
leaves damaged by A. alni larvae (A-neighbour) in an airtight container. The control leaves (C-neighbour)
were associated with only undamaged leaves. PI activity (mg PImg1 protein g1 FW) was monitored byradial diusion assay after 72 h of incubation. Arithmetic means + one standard error are given (n 6).FW=fresh weight. Undamaged leaves (A-neighbour) neighbouring damaged leaves (A. alni) showed
a signicant dierence from control leaves (Control and C-neighbour): t 3:12; n 18; p 0:0065 (orA-neighbour vs. C-neighbour: t 3:22; n 12; p 0:009). Damaged leaves and their undamagedneighbours did also dier (A. alni vs. A-neighbour): t 2:85; n 12; p 0:017:
T. Tscharntke et al. / Biochemical Systematics and Ecology 29 (2001) 10251047 1039
et al., 1984), or (iii) the attraction of natural enemies such as parasitoids andpredators (e.g. Dicke, 1994; Bruin et al., 1992; Turlings and Benrey, 1998). Possibleexplanations for this pattern also include a soil-borne signal transfer (e.g. Simardet al., 1997) and an intensied nutrient absorption of defoliated trees reducing foliagequality of undamaged neighbours (Tuomi et al., 1990, see Dolch and Tscharntke,2000). In the alder-leaf beetle interaction, responses of natural enemies to volatilesdid not appear to be of importance, because (i) the many rearings did not result inparasitoids and (ii) the eld samples of all arthropods associated with alder leavesdid not show an aggregation of natural enemies near the defoliated tree, but just theopposite pattern, since the number of natural enemies as well as predatorprey ratiosincreased with increasing distance to the defoliated tree. Interestingly, the specialistherbivores responded similarly to the defoliation as the alder leaf beetle, which isalso a specialist. In contrast, the generalists did not show this pattern, supporting theview that specialists are more aected by plant resistance than generalists (Maddoxand Root, 1987).Mechanisms that might have produced the patterns found in these eld
experiments were studied in more detail using biochemical analyses and furtherbioassays. These lab experiments had, for practical reasons, to rely on very youngalder trees, although such fast-growing, young trees can be expected to invest moreenergy in growth rather than in defense (Coley et al., 1985; Karban, 1990). Dolchand Tscharntke (2000) found that damage on black alders caused by the leaf beetleswas negatively correlated with tree diameter, so the young trees in the eld weremore damaged than old trees. Accordingly, the biochemical analyses and labexperiments may underestimate defensive responses which can be expected to bemore pronounced in the old (but hard to handle) trees.Results of the laboratory studies on the mechanisms involved in the induced
resistance of black alders following attack of the alder leaf beetle suggest adierentiation between direct, feeding-induced and indirect, volatile-induced plantresponses.
4.1. Direct, feeding-induced plant responses
Black alder proved to exhibit a multitude of inducible defense mechanisms as adirect response of alder foliage to feeding of A. alni. Results of this study showedincreases in the phenolic contents, in the activity of oxidative enzymes (PPO, LOX,and POD) and in proteinase inhibitors.The total amount of phenolics showed a 2-fold increase, supporting the results of
Oleksyn et al. (1998) and the general nding that leaves of the genus Alnus are rich inphenolic compounds (Hegnauer, 1964). The presumed role of phenolics ascomponents of plant defense is based on studies demonstrating their toxicity toherbivores when incorporated into articial diets (Elliger et al., 1981) and on thecorrelation of the phenolic content of plants with the amount of herbivory (Dudtand Shure, 1994).Anti-nutritive oxidative enzymes were also shown to increase in response to
herbivory. PPO is a major anti-nutritive protein, known to be induced by the
T. Tscharntke et al. / Biochemical Systematics and Ecology 29 (2001) 102510471040
jasmonic acid pathway, e.g. in tobacco and other solanaceous plants, and to causeresistance against several herbivores (Felton et al., 1992; Constabel and Ryan, 1998).LOX activity increased after herbivory and may also play a role in herbivore control.Damage to cells by feeding can cause the release of fatty acids including linoleic,linolenic, and arachidonic acids from membranes and other lipids (Gardner, 1980;Vernooy-Gerritsen et al., 1983). LOX will catalyse the oxidation of thepolyunsaturated fatty acids to form peroxy radicals, hydroperoxides, and superoxideanions (Gardner, 1980; Rustin et al., 1983; Lynch and Thompson, 1984). The freeradicals are highly reactive and can promote lignication, which may represent abarrier to some plant pests. LOX can also catalyze the production of JA fromlinolenic acid, which stimulates the expression of defense-related genes serving assecondary signals activating a subset of defense genes (see Farmer et al., 1992).Activity of the prooxidant enzyme POD was found to also increase with A. alnifeeding. Numerous studies have indicated that POD increases with insect attack orleaf tissue damage (Thaler et al., 1996). This response may be due to a direct role inplant resistance mechanisms. POD is assumed to be involved in the production oftoxic oxidative metabolites (Garner, 1984) and may promote lignication (Egleyet al., 1983). However, POD normally increases during tissue senescence in plantsand the increased concentration of lipid peroxides may simply be a response to pest-induced senescence of tissues (Matkovics et al., 1981). CAT, like POD, catalyses thereduction of toxic intermediates of the O2 metabolism (Faccioli, 1979). CAT activitydid not change after A. alni feeding. There was no response to herbivory in otherstudies (Faccioli, 1979; Matkovics et al., 1981), too, or even decreased activity insoybeans after herbivory (Bi and Felton, 1995).Alder plants could be also shown to produce proteinase inhibitors. The PI activity
increased upon feeding 5-fold. Proteinase inhibitors are found in a wide range ofplant species and their importance as anti-herbivore compounds against insectherbivory is well known (Koiwa et al., 1997, but see Jongsma et al., 1995). PIs mayinhibit digestive enzymes and reduce body mass indices (growth, weight) (Broadway,1995; Heath et al., 1997). Alnus incana, the grey alder, which is closely related to theblack alder A. glutinosa, is known to produce inducible PIs, and the induction of PIactivity in grey alder foliage has been shown to cause retarded growth, delayedpupation, reduced egg production and low survival of the leaf beetle Galerucellalineola (Seldal et al., 1994).
4.2. Indirect, volatile-induced plant responses
Only few publications give evidence for volatile signals triggering interplantcommunication following herbivory (Bruin et al., 1992; Arimura et al., 2000; Karbanet al., 2000). Black alder responded to insect damage with the release of volatiles. Therst signal after herbivory was the release of the phytohormone ethylene from thewounded leaves, and ethylene production increased 7-fold in response to herbivory.Ethylene is known to play important regulatory roles in primary and secondaryplant metabolism (Abeles et al., 1992). In black alder, the volatile ethylene appearedto be an important candidate of the possible interplant signal transfer following
T. Tscharntke et al. / Biochemical Systematics and Ecology 29 (2001) 10251047 1041
herbivore feeding and of the induction of plant resistance mechanisms. ODonnellet al. (1996) suggested that the initial production of ethylene (in tomato) may act aspositive-feedback mechanism to increase jasmonate synthesis linked to wounding.Feeding of A. alni induced not only ethylene, but also the biosynthesis of a large
number of other volatiles. Presumably, the regurgitate of black alder leaf beetlecontains eliciting compounds inducing the production of volatiles, becausemechanical leaf wounding caused only a non-specic blend of volatiles in lowquantities (Thiessen, unpublished). The great importance of insect-specic elicitorsfor the emission of volatiles is known from many insects (Turlings et al., 1993;Mattiacci et al., 1994, 1995; Alborn et al., 1997; Par!e and Tumlinson, 1998; Arimuraet al., 2000). Preliminary chemical analyses of the regurgitate of A. alni showed thatit contains no volicitin (Thiessen, unpublished), which has been claimed to elicitvolatile biosynthesis in maize plants (Alborn et al., 1997).JA application to leaves mediates the induction of volatiles. The inducing eect of
JA is known for various woody and herbaceous plant species (Hopke et al., 1994;Boland et al., 1995; Dicke et al., 1999; Meiners and Hilker, 2000). Many plantsrespond to herbivory, the herbivores oral secretions and regurgitants, to jasmonateapplication or the treatment with cellulysin with a release of a complex blend ofvolatiles including mono-, sesqui- or homoterpenes, which is often rather specic tothe attacking herbivores or treatment (Turlings et al., 1995; Takabayashi and Dicke,1996; Alborn et al., 1997; Boland et al., 1998). In black alder, application of JAinduced a volatile blend that was similar but not identical to that emitted byherbivory (Thiessen, unpublished), which supports observations on lima beans(Dicke et al., 1999). Among the many volatiles, there are signals for rapidcommunication among stressed plants: b-ocimene, DMNT, and TMTT induce theexpression of pathogen-related (PR) genes and other defense genes in undamagedlima bean leaves after exposure to the volatiles (Arimura et al., 2000). We could showthat wounded plants of Alnus glutinosa also produce the volatiles b-ocimene,DMNT, TMTT and MeSA, so these volatile signals may have also been involved ina possible interplant communication in black alders.We exposed leaves to the gaseous elicitors ethylene, MeJA, and MeSA, generally
thought to be candidates for plantplant communication and induced resistance(Farmer and Ryan, 1990; Thaler et al., 1996; Shulaev et al., 1997; Boland et al., 1998;Karban and Baldwin, 1997; Karban et al., 2000). As we have no data for the actualvolatile concentrations in the eld (typically with old trees which can be expected toshow much stronger responses than the very young trees or even isolated leaves, usedin the laboratory assays), we used these experiments to simply test whether there areany plant responses. We found an increase of CAT and PI activities after exposure ofMeJA and an increase of phenolics and PI activity after ethylene treatment.However, there was no induction of PPO by ethylene or MeJA. This was unexpectedsince an induction of PPO activity by MeJA has been reported for a number ofplants (Farmer and Ryan, 1990; Constabel and Ryan, 1998). MeSA was not foundto signicantly change phenolic metabolism or protein activity in our black aldersystem. The active components in mediating the possible plantplant interaction inthe present study may include ethylene, b-ocimene, DMNT, and TMTT, but methyl
T. Tscharntke et al. / Biochemical Systematics and Ecology 29 (2001) 102510471042
jasmonate (MeJA) may have also been involved (although not, or not yet, detectedamong the released volatiles). The relative importance of these candidates forinterplant signal transfer in alders is still unclear.Further evidence for plantplant communication comes from our container
experiments, where undamaged leaves were exposed to volatiles produced byherbivore-damaged leaves in airtight chambers. Not only the damaged leaves, butalso the undamaged neighbours of damaged leaves showed a signicant increase inthe activity of proteinase inhibitors (Fig. 8). We cannot exclude that the possible CO2enrichment in the chambers, which might be expected from the beetles respiration,may have aected PI activity, but as shown previously for Eucalyptus foliage, greatlyelevated CO2 levels are required to shift the metabolism to a signicant extent(Lawler et al., 1997; McDonald et al., 1999). In addition, the PIs of A. glutinosa wereinducible not only after insect feeding, but also after the application of ethylene,MeJA and JA (Fig. 7). PIs have already been found to be inducible after applicationof plant hormones such as ethylene or MeJA in other systems (Pena Cortes et al.,1988; Farmer et al., 1992; Koiwa et al., 1997). Such interplant signal transfer has alsobeen shown in herbaceous plants. Lima beans become less susceptible to spider mitesafter exposure to volatiles from attacked conspecics (Bruin et al., 1992), probablydue to the activation of defense genes by volatile terpenoids (Arimura et al., 2000).Wild tobacco plants with clipped sagebrush neighbours had increased PPO levelsand experienced reduced leaf damage by grasshoppers and cutworms (Karban et al.,2000).Results of our experiments suggest the following scenario of herbivore-induced
plantplant interactions among black alder trees. The alders respond to feeding of A.alni with an emission of ethylene and a blend of volatiles, followed by an activationof the JA signalling pathway. As a result, attacked alder leaves show increases (i) inthe phenolic content of the leaves, (ii) in the activity of oxidative enzymes (PPO,LOX and POD), and (iii) in the level of PIs. When exposed to ethylene, the phenoliccontent and the level of PIs were even induced in undamaged alder trees. However,we need more experiments to solve some inconsistencies, such as the increase in PIactivity, but not in phenolics (Thiessen, unpublished) when leaves are exposed to thevolatiles emitted by damaged neighbours.In conclusion, eld experiments, bioassays in the laboratory as well as biochemical
analyses suggest the existence of interplant resistance transfer in Alnus glutinosa, withairborne volatiles as a possible mechanism. However, we did not show the relativeimportance of airborne and possible soil-borne signals as well as unknown eects ofreduced foliage quality of neighbouring trees, due to a possible soil nutrientdepletion as a result of compensatory growth after defoliation (Dolch andTscharntke, 2000).
Comments of Ian T. Baldwin, Jan Bruin, and Marcel Dicke improved the papergreatly. We would like to thank Frank K .uhnemann (Institute for Applied Physics,
T. Tscharntke et al. / Biochemical Systematics and Ecology 29 (2001) 10251047 1043
University of Bonn) for his help with the photoacoustic laser spectrometer. Financialsupport came from the German Science Foundation (Deutsche Forschungsge-meinschaft, DFG).
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