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HematopoeisisVIBS 443/602
Peripheral blood smear (May-Grunwald-Giemsa)113
Peripheral blood smear (Leishman-Giemsa)eosinophil, basophil, and neutrophils
110
Bone marrow 1976119761
Bone marrow smear; low power view, showing negative images of fat globules
Megakaryocyte: contain many joined nuclei. Each probably contain 8-32 times a normal diploid amount of DNA
1. Nuclei of megakaryocyte
2. Red blood cell
Macrophage: contains greenish, iron-containing granules due to poor quality. Differentiating cells have been torn away during preparation.
1. Macrophage
2. Lymphocyte
3. Neutrophil
1. Pronormoblast
2. Basophilic normoblast
3. Polychromatophilic normoblast
4. Orthochromatic normoblast
5. Myelocyte
6. Late metamyelocytes
1. Pronormoblast
2. Basophilic normoblast
3. Polychromatophilic normoblast
4. Orthochromatic normoblast
5. Myelocyte
6. Late metamyelocytes
1. Late promyelocyte
2. Late myelocyte/early metamyelocyte
3. Nearly mature PMN
1. Smudge cell
2. Promyelocyte
3. Myelocyte
4. Metamyelocytes
1. Pronormoblast
2. Metamyelocytes
3. Band cell
Bone marrow: EM 12a
• Reticular cell
• Developing red blood cell
Bone marrow; EM 8a
1. Lymphocyte
2. PNM
3. Red blood cell
4. eosinophil
Bone marrow; EM 12b
1. PMN
2. Immature neutrophil
3. Red blood cell
Routine H&E of bone
• Marrow spicules
• Lipid in fat cells
H&E of bone marrow
1. megakaryocytes
19761
H&E of bone marrow:
• Red blood cell
Rib 420
Rib 420
Tibia, fetal421
32583
32583
Red bone marrow smear (May-Grunwald-Giemsa)112
Pronormoblastround nucleus and thin rim of deep blue cytoplasm
Basophilic normoblastnuclear condensation ("fractured nucleus") with a deep blue cytoplasm
Cells of Erythropoisesis
Polychromatophilic normoblastcytoplasm has accumulated enough hemoglobin so that it is no longer blue but a bluish-gray to bluish-red
Orthochromatic normoblastmust have an almost mature cytoplasm with only the faintest hint of blue: the nucleus is fully condensed
Reticulocyteno nucleus, mature cytoplasm with blue tint
Red blood celltypical of mature cell seen in circulation
Myelocytecytoplasm becomes increasingly mature (reddish/clear) but still contains blue, nucleus of variable shape
Cells of Granulopoiesis
Promyelocyteoval nucleus with deep blue cytoplasm and azurophilic granules
Metamyelocytedeeply indented nucleus with nearly mature cytoplasm (no longer blue)
Band cellnucleus just prior to lobulation with fully mature cytoplasm
Neutrophil (mature)lobulated nucleus with fully mature cytoplasm