1. INTRODUCTION: Construction of urinary bladder wall de novo for cancer patients with tissue engineering approach disqualify the use of autologous urothelial cells. 2. AIM: The goal of this study was to analyze if adipose derived stem cells (ADSCs) can transdifferentiate into urothelial- like cells (U-LCs) and serve potentially to construct urinary bladder wall in vitro.

The present work was supported by the National Center for Research and Development in Poland under Agreement no STRATEGMED1/235368/8/NCBR/2014 (Smart AUCI Project) within the Strategic Programme STRATEGMED “Prevention practices and treatment of civilization diseases”.

ADSCs+U-CM

NC+SM

NC+U-FM

PC+U-FM

Before the differen�a�on A�er 7 days A�er 14 days

Adipose derived stem cells (ADSCs)

+ a standard medium used for ADSCs culture (SM)

with the addi�on of urothelium derived condi�oned medium (U-CM)

= the differen�a�on (ADSCs + U-CM)

+ SM = the 1st nega�ve control (NC + SM)

+ SM with the addi�on of fresh medium used for UCs culture (U-FM)

= the 2nd NC (NC + U-FM)

HAVE ADIPOSE DERIVED STEM CELLS POTENTIAL TO TRANSDIFFERENTIATE INTO UROTHELIAL CELLS?

Buhl M, Rasmus M, Jundzill A, Balcerczyk D, Kloskowski T, Szeliski K, Dąbrowski P, Drewa T, Pokrywczynska M Department of Regenerative Medicine, Nicolaus Copernicus University in Torun, Ludwik Rydygier Medical College in Bydgoszcz, Poland

DIFFERENTIATION

7 OR 14 DAYS

ADSCs U-LCs?

3. MATERIALS AND METHODS:

DIFFERENTIATION: + a standard medium used for ADSCs culture (SM) with the addition of urothelium derived conditioned medium (U-CM) =ADSCs + U-CM

1st NEGATIVE CONTROL (NC): + SM =NC + SM

2nd NEGATIVE CONTROL (NC): SM with the addition of fresh medium used for urothelial cells (UCs) culture (U-FM) =NC + U-FM

ADSCs

UCs

4. RESULTS - CELL MORPHOLOGY:

0 7 14

AD

SCs

+ U

-CM

N

C +

SM

N

C +

U-F

M

PC

+ U

-FM

4. REAL-TIME PCR:

POSITIVE CONTROL (PC): + the standard, fresh medium used for UCs culture (U-FM) =PC + U-FM

ADSCs+

U-C

M

NC+S

M

NC+S

M

NC+U

-FM

PC+U

-FM

0

20

40

60

80

100

ADSCs+

U-C

M

NC+U

-FM

PC+U

-FM

14 days CK8

CK19

CK18

UP1b

CD44

CD105

Th

e r

ela

tive e

xp

ressio

n

0

20

40

60

80

100 7 days5. CONCLUSIONS: The study showed that ADSCs have no potential to transdifferentiate into urothelial- like cells and therefore there is a need to improve this method of transdifferentiation or search another type of stem cells to create urothelial cells for construction of urinary bladder wall de novo.