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Harvard Medical School/Harvard School of Dental Medicine

Format for the Curriculum Vitae

Date Prepared: January 2020

Name: Francis William (Bill) Luscinskas

Office Address: Department of Pathology, Brigham and Women's Hospital 77 Avenue Louis Pasteur, NRB 752P Boston, MA. 02115

Home Address: 111 Allerton Road, Milton, MA 02186

Work Phone: 617- 525-4337

Work Email: fluscinskas@partners.org

Work FAX: 617-525-4333

Place of Birth: Springfield, MA

Education

1975-1978 BS Medical Technology/Biology Northeastern University, Boston, MA

1978 Medical Technologist Northeastern University, New England Deaconess Hospital, and New England Baptist Hospital, Boston, MA

1980-1986 2010

PhD MS

Biochemistry Honorary

Boston University, Boston, MA Harvard University, Boston, MA

Postdoctoral Training

1985 - 1989 Research Fellow in Pathology

Vascular Cell Biology (under Michael A. Gimbrone, Jr., M.D.

Brigham and Women’s Hospital, Boston, MA

Faculty Academic Appointments

1985 - 1989 Research Fellow Pathology Harvard Medical School, Boston, MA

1990 - 1992 Instructor Pathology Harvard Medical School 1992 - 1996 Assistant Professor Pathology Harvard Medical School 1997 - 2009 Associate Professor Pathology Harvard Medical School 2010 Professor Pathology Harvard Medical School

Appointments at Hospitals/Affiliated Institutions

1990 - present

Research Associate Pathology Brigham and Women's Hospital

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2001 -present

Associate Director Vascular Research Division Brigham and Women’s Hospital

2001 – present

Senior Research Scientist

Center of Excellence in Vascular Biology

Brigham and Women’s Hospital

Other Professional Positions

1/1997 - 6/1997

Ibedrola Visitores Profesores, Servico Inmulogia, Hospital de la Princesa, lab of Dr. Francisco Sanchez-Madrid

Madrid, Spain

Major Administrative Leadership Positions

Local

1996 -2002; 2008 - 2012

Vascular Research Division (VRD) Seminars, Director Organizer of speakers representing our 6 Faculty Members, 18 Postdoctoral Fellows and 25 other staff

Brigham and Women’s Hospital

1997 – present

Cell Biology Core, Director Oversight of core that provides human and rodent cultured cells from both vascular and non-vascular sources to 6 Faculty Members in the Center and neighboring institutions

Center of Excellence in Vascular Biology, Department of Pathology, Brigham and Women’s Hospital

2001-present

Associate Director Assist Director with strategic planning and day-to-day oversight of a 50-person research group composed of 6 Faculty Members and their laboratories consisting of Residents, Postdoctoral Research Fellows, Graduate Students, Research Assistants, and Administrative Staff

Vascular Research Division, Department of Pathology, Brigham and Women’s Hospital

Committee Service

Local

1997-2003 Member, Confocal Microscopy Facility Oversight Committee Purchase and establish Biorad two-photon confocal microscopy core facility in Vascular Research Division

Brigham and Women’s Hospital

2000-2003 Member, Technology Assessment and Development Committee Develop hospital-based research organization and infrastructure for basic and applied research projects

Brigham and Women’s Hospital

2001-2002 Member, Working Group Synergies Working Group, Brigham and

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Identify key technologies and service areas used in research on campus, recommend opportunities for consolidation and sharing of these for organization of research among hospital departments as part of occupying new research space in 2003 at the New Research Building

Women’s Hospital and Harvard Medical School

2002 - 2006 Member, Residency Recruitment and Selection Committee Interview resident candidates for Pathology Department academic training program; Participate in bimonthly meetings to discuss and rank candidates to formulate match list

Brigham and Women’s Hospital, Department of Pathology

2003 - 2006 Member, Executive Committee on Animals (ECOA) Design guidelines and rules for animal space utilization at the New Research Building (NRB) 77 Avenue Louis Pasteur

Brigham and Women’s Hospital

2003 - 2004 Member, Research Service Improvement Committee Identify service issues impacting the research community including organizing institutional support for instrumentation cores, identifying technologies for institutional support and postdoctoral training programs

Brigham and Women’s Hospital

2001- present 2018 -2021 2019 - present

Member, Steering Committee, Vascular Biology Seminar Series Organize speaker program for the weekly Seminars in Vascular Biology Institutional Animal Care and Use Committee (IACUC), Faculty member Promotions, Reappointments and Appointments Committee

Harvard Medical Area Brigham and Women’s Hospital Harvard Medical School, Boston, MA

National and International

1999 - 2002 Member, Program Committee American Society of Investigative Pathology (ASIP)

2001 Organizer and Chair, Blood Vessel Club

North American Vascular Biology Organization (NAVBO), Experimental Biology Meeting

2003 – 2005 Member, Peer Review Steering Committee Commission

American Heart Association

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2003 - 2006 Councilor (elected) North American Vascular Biology

Organization (NAVBO)

2005 - 2008 Member, Inflammation Study Section Arthritis Foundation

2008 -2015 Member, Program Committee (elected) American Society of Investigative

Pathology (ASIP)

2010 – 2015 2012- 2015

Member, External Advisory Committee to COBRE Grant NIH P20RR18758 Chair, Program Committee (elected)

Dr. Rodger McEver, Oklahoma Medical Research Foundation, and University of Oklahoma Medical Center, Oklahoma City, Oklahoma. American Society of Investigative Pathology (ASIP)

2011-2012 2013-2017 2015 - 2018 2015-2016 2017 – present 2017 – present 2020

President, Organized the Pluto Society Annual Meeting in 2012 Member, Co-Chair; co-Chair, Stand Alone Meeting Program Committee (PISA); Co-organizer of two Symposia in PISA 2015; Co-Chair, 2016; Chair 2017 – 19 Co-Chair, Stand Alone Meeting Program Committee for PISA 2016-18 Member, Planning Committee Co-Chair, Vascular and mucosal biology (VAMP) Scientific Interest Group Member, External Advisory Committee to COBRE Grant NIH P20RR18758 Stand Alone Meeting Program Committee for PISA 2020. Member

American Society of University Pathologists (Pluto Society) American Society of Investigative Pathology (ASIP) American Society of Investigative Pathology (ASIP) International Vascular Biology Meeting (IVBM 2016), Boston, MA American Society of Investigative Pathology (ASIP) Dr. Liji Xia, Oklahoma Medical Research Foundation, and University of Oklahoma Medical Center, Oklahoma City, Oklahoma American Society of Investigative Pathology (ASIP)

Professional Societies

1985- American Society for the Advancement of Science

1988- 1990 -

American Society for Investigative Pathology (ASIP) (formerly American Association of Pathologists) American Association of Immunologist (AAI) American Physiology Society (ASPS)

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2012 - 2015 1994- 2008 North American Vascular Biology

Organization

2008- Elected member, American Association of

University Pathologists (Pluto Society)

Grant Review Activities

1996,1997, 1998, 2010

Ad Hoc Peer Reviewer The Israel Science Foundation

1997 Ad Hoc Peer Reviewer Foundation for Pediatric Oncology Research, Groningen, Netherlands

1998-2002 Peer Reviewer, National Study Section

1999-2001 2001-2002

American Heart Association Co-Chairman, Vascular Wall Biology I Chairman, Vascular Wall Biology I

1999 Ad Hoc Peer Reviewer Alberta Lung Association, Edmonton,

Alberta, Canada

2000- present

Ad Hoc Peer Reviewer Program Project Review

NIH/NHLBI

2001, 2017, 2019

Ad Hoc Peer Reviewer The Welcome Trust Foundation, London, UK

2007 -2013 Peer Reviewer, Vascular Biology

2007-2009 2009-2013

NIH CVS Study Section Ad Hoc Member Chairman

2008 – 2018 2019 - present

Ad Hoc Peer Reviewer; Cardiac Biology/Regulation 1 Chair or Co-Chair 2015 -2018 Career Development Award Vascular Basic Sciences

American Heart Association (AHA) American Heart Association (AHA)

Editorial Activities

1993-1998 Editorial Board Member Microcirculation 1999 - 2002 Associate Editor Microcirculation 1996 - 2000 Primary Reviewer Journal of Immunology 1998 - 2000 Associate Editor Journal of Immunology 2000 - 2005 2010 - 2014 2012 - 2017 2012 - 2017

Section Editor Editorial Board Member Editorial Board Member Editorial Board Member

Journal of Immunology Microcirculation Tissue Barriers Cell Communication and Adhesion

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2015 – 2016 2015 – 2019

Advisory Board Editorial Board Cancer

Seminars in Immunopathology Microcirculation

Other Editorial Roles

1992- Ad Hoc Reviewer American Journal of Pathology 1992- American Journal of Physiology

1992- Blood

1992- Circulation Research

1996- European Journal of Immunology

1992- FASEB Journal

1995- Immunity

1992- Journal of Cell Biology

1992- Journal of Clinical Investigation

1992- Journal of Experimental Medicine

1992- Journal of Leukocyte Biology

1998- Cell

1998- Microcirculation 2003- Nature Immunology

2007- Molecular Biology of the Cell

Honors and Prizes

1976 - 1978 Deans' List College of Pharmacy and Allied Health, Northeastern University

Boston, MA

1978 Graduation with Honors

Northeastern University

1983 - 1984 NIH Predoctoral Training Grant

Boston University Medical School Boston, MA

1987 - 1990 Individual Postdoctoral Fellowship Award F32-HL07672

NIH

2000 Established Investigator Award

American Heart Association (Declined to accept RO-1 HL65090)

2001 Partners in Excellence Award

Partner’s HealthCare System, Inc. Team Award to Division for Recognition of Leadership and Innovation

2007 2012

Excellence in Tutorial Facilitation Visiting Professor, Center for Vascular Biology Research

Harvard Medical School Beth Israel Deaconess Medical Center, hosted by Dr. William Aird, November 14

Boston, MA

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Report of Funded and Unfunded Projects

Funding Information

Past

1987 - 1990 Endothelial-dependent Leukocyte Activation NIH NRSA F32 HL07672 PI

1992- 1996 Endothelial-dependent Leukocyte Activation NIH R29 HL47646 PI

1997 - 2007 Critical Transitions in Atherogenesis NIH P50HL 56985 PI: MA Gimbrone, Jr., Program Director Role: Co-Investigator; Project 3: Cellular Immune Mechanisms in Atherogenesis

1999 - 2004 Endothelial Cell Dysfunction in Chronic Immune Diseases NIH RO-1 HL65090 PI

2000 Established Investigator Award American Heart Association (declined to accept NIH RO-1 65090)

1993 - 2008 Pathophysiology of the Endothelium NIH PO1-HL36028-22 PI: MA Gimbrone, Jr., Program Director Role: PI; Project 2: Leukocyte Transendothelial Migration Pathways

1993 - 2008 Pathophysiology of the Endothelium NIH PO1-HL36028-22 PI: MA Gimbrone, Jr., Program Director Role: PI; Core A: Cell Biology Support

2003 - 2008 Pathophysiology of Endothelium NIH PO1-HL36028-22 PI: MA Gimbrone, Jr.; Program Director 1996-2010 Role: PI; Core D: Administrative Support

2009 - 2015 Pathophysiology of the Endothelium NIH PO1-HL36028-24 Program Director; PI; Project 1: The role of CD47 in mononuclear leukocyte recruitment

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2009 – 2015 2009 - 2015

2014-15 2015-2016

2015-2017 6/2017 – 9/2019

Pathophysiology of the Endothelium (NCE) NIH PO1-HL36028-24 Program Director; PI; Core A: Cell Biology Support Pathophysiology of the Endothelium (NCE) NIH PO1-HL36028-24 Program Director; PI; Core D: Administrative Support BWH Biomedical Research Institute (FW Luscinskas, PI). ($50,000/yr) CD47 Regulation of Leukocyte Integrins During Leukocyte Trafficking. American Heart Association, Award Number 15GRNT25080164 ($66,000/year). Principal Investigator: FW Luscinskas Luscinskas Lab Research Support “Breeding and characterization of CD47Fl/Fl x LysM-Cre mice”. ($40,000/year). University of Michigan (Prime PI: Dr. Charles Parkos) Principal Investigator: FW Luscinskas Sponsored Research Agreement (BWH # 2017D000361), Surface Oncology, Cambridge, MA. Direct costs, $60,135; Total cost $106,740

Current

5/2016- 3/2020 9/2018-9/2020 1/2019- 12/2019

CD47 Regulation of Leukocyte Integrins during Leukocyte Trafficking NIH R01-125780; Direct costs: $344,000 Principal Investigator: Francis W. Luscinskas BWH Oncologic Pathology pilot grant award ($15,000). Define the Efficacy of CD47 Blockade in Models of Experimental Autoimmune Encephalomyelitis (EAE). iAward Sanofi: Direct costs: $98,832 PI: Francis W. Luscinskas

Report of Local Teaching and Training

Teaching of Students in Courses

a. Harvard Medical School

I participate as a faculty member in a pathology course at Harvard Medical School. I have served as an Instructor and Section Leader in the laboratory component of the HST-030 course Human Pathology since 1996. The lab portion consists of 8 -10 case study conferences, problem sets, and two lab sessions per week (1.5 hr each), in which the histological features of normal and diseased human tissue and organ systems are taught to first year HST students (MD, DMS, PhD, and MD/PhD students).

1996- present

Human Pathology course (HST-030), 50-75 MD, MD, and PhD, and PhD

Division of Health Sciences and Technology, Harvard Medical School

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candidates

Laboratory Instructor and Case Instructor 60 hours/year (6 hrs/week,14-16 weeks; Fall semester);100 hr/year preparation time

1997-2011 Human Pathology course (HST-030), 50-75 MD, MD and PhD, and PhD candidates

Division of Health Sciences and Technology, Harvard Medical School Lecturer; Frontiers in Science Lecture 1 hr lecture on cutting edge topics in inflammation

1998 - 2014 Immunology, Microbiology and Pathology (IMP) Course (HMS-IN 714.0) MD, PhD and DDS candidates

Harvard Medical School Tutor; 36 direct contact hrs/Spring semester (6-8 students, attend lectures, provide student evaluations)

Laboratory and Other Research Supervisory and Training Responsibilities

1991 PhD Thesis Committee: Ms. Elizabeth Neuman (Thesis Advisor, Dr. Richard Jack)

2004 PhD Thesis Committee: Mr. Avi N. Kogan, Committee on Higher Degrees in Biophysics, Harvard University (Thesis Advisor, Dr. Uli von Andrian)

2005-2009 PhD Thesis Advisory Committee: Mr. Jean-Marc Gauquet, BBS Program (Thesis Advisor: Dr. Uli von Andrian)

2006-2011 PhD Thesis Advisory Committee: Mr. Noah Tubo, BBS Program (Thesis Advisor: Dr. James Campbell)

2009- 2013 PhD Thesis Advisory Committee: Ms. Suzanne Josette Taghap Nizza, BBS Program (Thesis Advisor: Dr. James Campbell)

2006-2011 PhD Thesis Defense Committee: Mr. Noah J. Tubo, BBS Program

2011- 2016 8/2014

PhD Thesis Advisory Committee: Mr. Kutay Karatepe, BPH Program PhD Thesis Defense Committee: Kimberly Lindsay Martinod, Committee on Immunology, HMS

1991- Supervisor, undergraduate, graduate, medical, and post-doctoral students:

Center for Excellence in Vascular Biology, Vascular Research Division, Brigham and

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training in laboratory techniques (biochemical analysis, immunological techniques, small animal surgeries and models of inflammation, adoptive cell transfer techniques); discussion of experiment results, graphing data and application of standard statistical analysis to data.

Women’s Hospital 1300 hrs/year (25 hr/week)

Supervisor, Research Fellows, students: training in writing scientific abstracts and manuscripts, research seminar presentation, and presentation organization for local, national, and international scientific meetings. Weekly lab meetings.

Other Institutions: PhD Thesis Committees

1996 M.Berman The Rockefeller University

1997 1) P S. Sanwar, PhD Thesis entitled “The role of selectins in leukocyte recruitment in vivo”

2)

The University of Calgary, Calgary, Canada

2002 3) Robert Tilghman, PhD thesis entitled “Signaling through endothelial cell adhesion molecules”

Department of Pathology, Vanderbilt University

Formally Supervised Trainees

Undergraduate

6/2002-9/2002

Mr. Leslie Lee, undergraduate biomedical engineering student, Northwestern University, Evanston, IL. Summer internship. Fourth year medical student at Columbia University, New York, NY

6/2004 – 9/2004

Ms. Susan Chen, pre-med undergraduate at Harvard College, Cambridge, MA. Summer internship

6/2009 – 5/2012 6/2014- 8/2014

Mr. Ben Weinberg, Undergraduate Student at Boston University School of Engineering, Boston, MA Tekeydra Lee, Jackson State University, Jackson, MS. A Brigham and Women’s Hospital sponsored program funded by an NIH grant for undergraduate students called “Summer Training in Academic Research and Scholarship” (STARS).

Graduate: Predoctoral research experience (summers, elective periods).

1990 – 1991 Mr. Christopher Peckins, HMS medical student. Currently internist at Mount Auburn Hospital, Cambridge, MA

1995 Ms. Maria Montoya, PhD student, University Autonoma de Madrid, Madrid, Spain.

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Currently director, Confocal Microscopy and Cytometry Unit, National Cancer Research Center, Madrid, Spain

2000 Mr. Tim J. Ryan, HMS, HST student, co-supervisor with Dr. Andrew Lichtman

2000 Anne Montague, PhD, University de Barcelona, Barcelona, Spain

2002-2003 Ms. Pilar Alcaide, PhD (2003, University Autonoma de Madrid, Madrid, Spain). Currently

an Assistant Professor, Tufts University, Boston, MA

2005 Ms. Sandra March, Ph.D student, University of Barcelona, Barcelona, Spain. Currently a

postdoctoral fellow at MIT

2006 Veronica Azcutia-Criado, PhD (2008, University Autonoma of Madrid, Madrid, Spain)

2007-2009 Monica Sircar, HMS, HST Program. Honor’s Thesis supervisor. Currently

resident, Sloan Memorial Hospital, New York, NY

6/2011-9/2011 9/2012-6/2013 6/2014 – 8/2014 6/2017 – 8/2017

Matthew Routledge, Imperial College London (School of Medicine), 3rd year medical student, 3 month research internship Nick Rizzo, Emmanuel College, Boston, MA. Senior Thesis (expected 6/2013) Tykeydra Lee, (BWH summer program STARS sponsored by NIH), rising Senior undergraduate student at Jackson State University, MS. Gustavo Gonzalez (BWH summer program STARS sponsored by NIH), second year medical student 6 yr BS/MD program at Brown University, Providence, RI

Doctoral research advisor/supervisor:

1989 – 1992 Dr. Jian Shen, MIT, Department of Mechanical Engineering; currently Senior Scientist, Ortho Diagnostics Systems, Johnson and Johnson, Princeton, NJ

1995-1996 Dr. Daniel Grief, Fellow of the Sarnoff Foundation; MD, Univ. of California, San Francisco, MD with thesis, 1997. Currently Instructor in Medicine, Stanford University Medical School, Palo Alto, CA

2002-2006

Dr. Aleksandr Rabodzey, MIT, Biological Engineering Department, PhD Thesis committee advisor and examiner. Currently a postdoctoral fellow in lab of Dr. Chenghua Gu, Harvard Medical School

2007-2008 2012-2013

Ms. Patrice Scipio, Fellow of the Sarnoff Foundation; third yr medical student at Ohio State University Medical School, OH Alexander Song, Boston University Graduate School, MS degree, candidate )MS thesis in my laboratory, expected 6/2013)

Post-doctoral trainees: Previous:

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1992-1993 Pilar Pizcueta, PhD, currently Associate Professor, University of Barcelona, Barcelona, Spain

1994-1995 Peter Tan, BM, FRCS, currently a practicing vascular surgeon, Austerfield, England

1995-1997 Douglas Goetz, PhD, currently Professor, Chemical and Biomolecular Engineering Department, Ohio University, Athens, OH

1996-2000 Jennifer Allport, PhD, currently Senior Research Investigator I, Laboratory Head, Cardiovascular Disease, Novartis Institutes for BioMedical Research, Cambridge, MA

2000-2002 Shuo Ma, MD, PhD, Assistant Professor of Medicine, Department of Medicine, Northwestern University School of Medicine, Chicago, IL

1996-2003 Yaw-Chyn Lim, PhD, Assistant Professor, Departments of Physiology and Pathology, National University of Singapore, Singapore

2002-2004 Jin Zhang, PhD, currently Research Scientist, Proctor and Gamble, Englewood, OH

2002-2004 Ravi Rao, MA, MRCP, PhD., currently Translational Medical Leader, Roche Products Limited, Shire Park Welwyn Garden City, Herts, England

1997-2004 Sunil Shaw, PhD, currently Assistant Professor, The Warren Alpert Medical School of Brown University; Research Scientist, Department of Pediatrics, Women and Infants Hospital, Providence, RI

2003-2005 Mike Kim, PhD, currently Manager, Business Development at Alkermes, Inc., Cambridge, MA 02139

2003-2005 Angeles Alvarez, currently Assistant Professor, University of Valencia, Valencia, Spain

2002-2006 Lin Yang, MD, PhD, currently Senior Scientist, Wyeth-Pfizer Nutrition, Collegeville, PA

2005-2008 Scott Auerbach, PhD, currently Research Associate, Salk Institute, La Jolla, CA

2006 - 2009 Michael A Stefanidakis, PhD, Associate Director, Head of Pharmacology, Editas Medicine, Cambridge MA.

2004 – 2011 2006-2013 2009 - 2010 2009 - 2013

Pilar Alcaide, PhD, currently Associate Professor (tenured) of Medicine, Tupper Institute of Cardiovascular Research, Tufts University School of Medicine, Boston, MA. She has been awarded a NIH K99/R00 grant (HL94706). Dr. Luscinskas served as her mentor in Yrs 1 and 2 (2009 – 2011)

Karen Westerman, PhD, currently Assistant Professor of Medicine, Harvard Medical School, Department of Anesthesiology, BWH. She was awarded a NIH K0-1 award. Dr. Luscinskas served as a co-mentor on this award. He is also listed as a consultant on her recently submitted NIH R0-1 award.

Roberta Martinelli, PhD currently Senior Scientist, Merck Pharmaceutical, Boston, MA. Marcie R. Williams, PhD, currently Secondary School teacher, Nashville TN

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2012 - 2014 2009-2015 2015-2018 -

Marion Leick, PhD, currently Vice President, LuxDoc a.s.b.l. Veronica Criado Azcutia, PhD currently Research Investigator (Research Track) in the Department of Pathology, University of Michigan Medical School, Ann Arbor, MI. Anu Antio, PhD, currently Postdoctoral Fellow in Pathology, BWH

2017-2019 Francisco E. Velázquez Planas, currently Postdoctoral Fellow in Pathology PhD, BWH

Supported by Departmental Training grant.

Formal Teaching of Peers (e.g., CME and other continuing education courses)

Local Invited Presentations

Report of Local, Regional, National and International Invited Teaching

and Presentations

Invited Presentations and Courses

Regional

1995 Mononuclear leukocyte interactions with vascular endothelium under flow: models and mechanisms”, Invited lecture, The Rockefeller University, Laboratory of Cellular Physiology and Immunology, New York, NY. Hosted by Dr. William Muller, January 18

“Mononuclear leukocyte interactions with vascular endothelium under flow: models and mechanisms”, Invited speaker, College of Physicians & Surgeons of Columbia University, Department of Physiology and Cellular Biophysics, New York, NY. Hosted by Dr. Sam Silverstein, January 17

1997 “Role of endothelium in leukocyte transmigration”, Brigham and Women's Hospital, Invited Seminar at the Center for Experimental Therapeutics and Reperfusion Injury. Hosted by Drs. Charles Serhan and Sean Colgan

“Endothelial dependent mechanisms of leukocyte transmigration, Phagocytes Gordon Research Conference, Holderness, NH, June 12

1998 “Endothelial dependent mechanisms of leukocyte transmigrationm", Invited Plenary Presentation, Keystone Symposia Conference, Endothelium and Molecular Mechanisms of Leukocyte Trafficking, Lake Tahoe, NV. Organized by Dr. Sam Silverstein, March 23

“Endothelial cell lateral junctions and leukocyte trafficking". Invited Seminar, Beth Israel Hospital, Department of Pathology, Boston, MA. Hosted by Drs. Hal Dvorak and Jack Lawler, January 23

“Endothelial cell lateral junctions and leukocyte trafficking." Invited Seminar, Research

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seminar, Presented at Harvard Medical School, Seminar in Vascular Biology, Boston, MA. Hosted by Dr. Michael A. Gimbrone, May 21

“Molecular mechanisms of leukocyte-endothelial cell adhesive interactions in vitro” Invited Seminar, Massachusetts General Hospital Gastrointestinal Unit, Boston MA. Hosted by Dr. Timothy Wang

1999 Leukocyte-endothelial cell adhesion and transmigration." Invited research seminar, the Center for Blood Research, Boston, MA. Hosted by Drs. U. von Andrian and E. Quackenbush, February 23

“The role of chemokines in monocyte arrest and transmigration across activated endothelium under flow." Invited seminar, Leukocyte, Inc. Cambridge, MA. Hosted by Dr. Mike Briskin, March 24

“Role of Endothelium and lateral junctions in leukocyte recruitment in vitro”. Invited Seminar. Department of Pathology, Brigham and Women’s Hospital, Boston, MA, Research Conference, October 26

2001 “Regulation of Endothelial Cell-Cell Adhesion During Inflammation” Invited seminar,

presented at SECTION MEETING, Biochemistry/Biochemical Pharmacology Discussion Group, New York Academy of Sciences. Minisymposium: New Insights into Inflammation: Novel Cellular and Molecular Aspects of Leukocyte-Endothelial Cell Interactions. Organized by Dr. William A. Muller, Weill Medical College of Cornell University, January 30

2002 “Role of endothelial cell lateral junctions in leukocyte transmigration”, Invited research

seminar, the Department of Pathology, Weill College of Medicine, Cornell University, New York, NY. Hosted by Dr. William A. Muller, May 14

“Models of endothelial cell dependent mechanisms for leukocyte egress from blood vessel

wall in inflammation”, Invited research seminar, the Center for Molecular Medicine, Maine Medical Center Hospital, Portland, ME. Hosted by Drs. Tom Maciag and Volkhard Linder, June 6

“Leukocyte trafficking: a junctional affair", Invited Research Seminar, Harvard Medical

School, Seminar in Vascular Biology, Boston, MA. Hosted by Dr. Michael A. Gimbrone, October 10

2004 “Leukocyte LFA-1 and ICAM-1 localization during transmigration: Lessons from live cell

imaging studies”, invited research seminar, Eyetech Pharmaceuticals, Woburn, MA. Hosted by Drs. Tony Adamis and Greg Robinson, September 25

2005 “Molecular mechanisms that control T cell adhesion under shear flow: Live cell imaging

approaches”, Invited research seminar, Gastrointestinal Division, Massachusetts General Hospital, Boston, MA. Hosted by Dr. Hans Reineiker, December 13

2006 “New connections for endothelial cell ICAM-1 in leukocyte recruitment.” Invited seminar,

Harvard Medical School, Seminar in Vascular Biology, Boston, MA. Hosted by Dr. William Aird, October 10

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2007 “ICAM-1 connections during leukocyte transmigration.” Invited seminar, Center for Immunology and Inflammatory Diseases, Massachusetts General Hospital, Boston, MA. Hosted by Dr. Cathryn Nagler-Anderson, October 11

“Endothelial cell control points for leukocyte trafficking: Role of ICAM-1 dependent

signals”, Invited seminar, Department of Biomedical Engineering, University of Rochester, Rochester, NY. Hosted by Professor Richard E. Waugh, March 22

2008 “Adhesion dependent signaling and mechanical forces induced during leukocyte

diapedesis." Invited seminar, Interdepartmental Program in Vascular Biology and Therapeutics, Yale University School of Medicine, New Haven, CT. Organized by Dr. Themis Kyriakides, March 24

2009 “CD47 integrin associated protein and in T cell recruitment” Invited seminar, Joint Dana-Farber Cancer Institute-Brigham and Women’s Hospital–Joslin Diabetes Center Immunology Research Seminar. Organized by Dr. Mike Gurish and Howard Katz, February 23

“A new look for CD47 integrin associated protein in T cell recruitment.” Invited speaker, the Brigham and Women’s Hospital Biomedical Research Institute Infectious and Immunologic Diseases Research Center, February 23

2011 2012 2013 2014 2016 2018 2019

“Leukocyte recruitment to the vascular wall: Mechanistic insights.” Invited speaker, Pathology Grand Rounds, Department of Pathology, Brigham and Women’s Hospital, March 11 “CD47: an important modulator of the innate and adaptive immune systems during inflammation”. Visiting Professor Lecture, Center for Vascular Biology Research, Beth Israel Deaconess Medical Center, Boston, MA. Hosted by Dr. William Aird, November 14

“CD47 regulates 2 integrin functions in models of inflammation” Invited seminar, Joint Dana-Farber Cancer Institute-Brigham and Women’s Hospital–Joslin Diabetes Center Immunology Research Seminar. Organized by Dr. Mike Gurish and Howard Katz, September 30. “Regulation of T cell trafficking by CD47, a β2 integrin associated molecule” Visiting Professor, Sackler School of Graduate Biomedical Sciences, Program in Immunology, Tufts University School of Medicine. Organized by Dr. Henry Wortis. February 19. “CD47 and ENMC in Innate and Adaptive Immune Responses.” presented at the Whitaker Cardiovascular Institute, Boston University School of Medicine, Invited by Dr. Katya Ravid, May 17.

“Molecules and Molecular Events During Immune Cell Recruitment to Sites of Inflammation.” Invited speaker, Pathology Grand Rounds, Department of Pathology, Brigham and Women’s Hospital, November 14.

“Assessing CD47 as a therapeutic target in inflammatory disease.” Invited speaker, Cardiovascular, Diabetes, and Metabolic Disorders Research Center, Frontiers in Biomedical Science Seminar Series, November 18.

“Targeting CD47 to modulate leukocyte trafficking and immune cell activation for

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2019 therapeutic impact’ Plenary Lecture at the XXI Boston Angiogenesis Meeting (BAM), Tufts Medical School, November 22, 2019.

National

1991 “Involvement of ELAM-1 and ICAM-1 in neutrophil adhesion and transmigration across vascular endothelium”, Invited speaker, Fifth World Congress for Microcirculation, Louisville, KY

1992 “Monocyte attachment to Interleukin-4-activated endothelium is mediated via L-selectin and Vascular Adhesion Molecule-1 under non-static conditions”, oral presentation, American Heart Association Annual Meeting, New Orleans, LA

1993 “Human monocytes roll and attach to Interleukin-4-activated endothelium under physiological levels of flow”, Invited oral presentation, Experimental Biology 93, New Orleans, LA

1995 L-selectin and P-selectin preferentially mediate monocyte rolling and adhesion to TNF-α

activated vascular endothelium under flow”, Invited presentation, Experimental Biology 1995, Atlanta, GA

1996 “Endothelial cell regulation of leukocyte transmigration”, Invited lecture, Immune/Non-Immune Cell Interactions in Intestinal Inflammation, sponsored by Crohn's & Colitis Foundation of America, American Gastroenterological Association and National Institutes of Health, Tampa, FL

“Endothelial-dependent mechanisms of leukocyte migration: Novel role of the proteasome,” Invited speaker, IX International Vascular Biology Meeting, Seattle, WA

1997 “Endothelial dependent mechanisms of mononuclear leukocyte adhesion and transmigration”, Invited Seminar, The University of Virginia, Charlottesville, VA. Hosted by Dr. Klaus Ley

“Role of endothelium during leukocyte transmigration”, Invited Seminar, Louisiana State University, Shrevesport, LA. Hosted by Dr. Neil Granger, Department of Physiology, September 29

Co-chairman, ASIP Section, Experimental Biology 97, New Orleans, LA, entitled “Leukocyte-endothelial interactions,” April 7

“The role of endothelial cell lateral junctions in leukocyte transmigration in vitro", Invited speaker, American Gastroenterological Association Meeting, Washington, DC, sponsored by the National Institutes of Health, May 13

1998 “Endothelial dependent mechanisms of monocyte adhesion and transmigration in vitro”, Invited Plenary speaker, FASEB Summer Research Conference on Physiology and Pathophysiology of the Splanchnic Circulation, Wilsonville, OR, July 21

1999 “The role of chemokines in mononuclear leukocyte interactions with endothelium under flow." Invited research seminar, Emory University, Department of Pathology, Atlanta, GA. Hosted by Dr. Charles Parkos, April 5-7

“Role of the Polymophonuclear Leukocyte in Sickle Cell Disease”, Participant/Invited Speaker, NIH/NHLBI Workshop

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Co-chairman, AAI Section, Experimental Biology 1999, Washington DC. Mini-Symposia

entitled "Chemokines and leukocyte interactions,” April 19

New molecular Insights into the basis of leukocyte trafficking and migration”, Invited Plenary Speaker, Gastroenterology Research Group/American Gastroenterology Association Symposium entitled Cell-Cell Interactions, Orlando, FL. Hosted Drs. Kim Barrett and Gail Hecht, May 15

2000 “ASIP Minisymposium”, invited as Chairman of Session entitled “Leukocyte-endothelial cell interactions”. Experimental Biology 2000, April 15 - 19

“Endothelial cell dependent mechanisms of chronic inflammatory leukocyte transmigration”. Invited Plenary Speaker, Transplant 2000, sponsored by The American Society of Transplantation and American Society of Transplant Surgeons, Chicago, IL, May 13-17

“Leukocyte-Endothelial interactions: The beginning and the end:, Genentech, Inc., Invited Research Seminar, San Francisco, CA. Hosted by Dr. Mary Gerritsen, December 6

2001 “Dynamics of endothelial cells during transmigration”, invited plenary speaker, the ASIP Symposia “New Mechanisms of Leukocyte Transmigration”, Experimental Biology 2001, Orlando FL, March 30-April 3

“New mechanisms of leukocyte transmigration”, American Society of Investigative Pathlogy, Major Symposia, Chairman and Organizer, Experimental Biology 2001, Orlando FL, March 30-April 3

“Regulation of leukocyte adhesion and transendothelial cell migration”. Invited research seminar, Biomedical Engineering Seminar Series. Sponsored by Departments of Biomedical Engineering and Chemistry, and College of Medicine, Ohio University, Athens OH. Hosted by Dr. Douglas Goetz, April 30

“Is there a role for endothelial junctional control during leukocyte egress?” Invited Speaker, the annual Gelber Lecturer, Research Seminar, Department of Medicine, Baylor College of Medicine, Houston, TX. Hosted by Dr. C. Wayne Smith, June 25-26

2002 “Models and mechanisms in leukocyte egress from the blood vessel in inflammation”, Invited Research Seminar, the Department of Pathology, Vanderbilt University, Nashville, TN, May 31. Hosted by Dr. Richard Hoover, Professor of Pathology

2003 “How leukocyte emigrate the vascular endothelium”, Invited Research Seminar, Department of Pharmacology, University of Illinois College of Medicine, Chicago, IL. Hosted by Dr. Asrar Malik, December 2

2004 “Leukocyte-endothelial cell interactions: From start to finish”, Pathology Grand Rounds presentation, University of Texas, Galveston, TX. Hosted by Dr. David Walker, January 12

“Endothelial cell dependent mechanisms that regulate leukocyte recruitment”, Invited Research Seminar. Hosted by Dr. Klaus F. Ley, The Cardiovascular Research Center, University of Virginia, Charlottesville, VA, January 15

18

“Leukocyte transendothelial cell migration: Is it a strictly junctional affair”, Invited Research Seminar, Departments of Pathology and Cell Adhesion and Matrix Adhesion Center, University of Alabama at Birmingham. Hosted by Dr. Dan Bullard, January 22

“Leukocyte-endothelial cell interactions: Lessons from live cell imaging, Invited Speaker, Pathology Grand Rounds, Duke University, Durham, NC. Hosted by Drs. Salvadore Pizzo and Thomas F. Tedder, June 25

2005 “Endothelial cell dependent mechanisms of leukocyte recruitment: Lessons learned from live cell imaging”, Research seminar, Department of Pathology and Immunobiology, University of Cincinnati, Cincinnati, OH. Hosted by Dr. Joan Cook-Mills, January 19

“New Insights into the molecular mechanisms of leukocyte recruitment using live cell imaging approaches”, Distinguished Lecture, Research Forum, Oklahoma Medical Research Foundation (OMRF). Invited lecture, Hosted by Dr. Rodger McEver, Vice President of Research and Member, OMRF, September 21

2006 “New insights into leukocyte recruitment using live cell imaging approaches” Plenary presentation, sponsored by NAVBO and American Society of Investigative Pathology, Experimental Biology Meeting. Organized by Dr. Cathryn Cathcart, April 4

2007 “VE-cadherin and p-120 catenin: Surprising gatekeepers of leukocyte transmigration” and “What’s new in the basic steps in leukocyte transmigration.” Invited seminars, First Symposium on The Microcirculation in Acute Viral and Bacterial Infections, University of Texas Medical Branch, Galveston, Texas. Organized by Drs. David H Walker, Charles Fulhorst and Gustavo Valbuena, November 22

“VE-cadherin and p-120 catenin: Surprising gatekeepers of leukocyte transendothelial migration”. Invited seminar, Institute for Environmental Medicine, University of Pennsylvania Health System, Philadelphia, PA. Hosted by Dr. Aron B. Fisher, June 22

2008

“New players in endothelial cell dependent mechanisms of leukocyte transmigration“,

Plenary presentation, ASIP Symposium “Intracellular Transport in Vascular Cells”, Experimental Biology 2007. Organized by Drs. Lowell Langille and Avrum Gotlieb, May 1

2009 “Molecular Mechanisms Controlling Leukocyte Diapedesis.” Invited seminar, Pluto Society Meeting, Los Suenos, Costa Rica, March 8

Chairman of ASIP sponsored Symposia entitled “Patrolling the Vascular Interface by Leukocytes”, Experimental Biology meeting, New Orleans, LA, April 19

“Endothelial Cell Regulation of Leukocyte Diapedesis”, Invited speaker, ASIP Symposia “Molecular mechanisms and dynamics of leukocytes breaching tissue barriers” presented at the Experimental Biology 2009 meeting, New Orleans, LA, April 21

“Adhesion and Migration”, Invited seminar, University of North Carolina, Chapel Hill. Organized by Dr. Keith Burridge, April 25

2011 “CD47: A multifunctional adhesion molecule that rolls, arrests and transmigrates.” Invited speaker, Weill Medical College and Graduate School of Medical Sciences, Cornell University, New York, NY. Hosted by Dr. David Hajjar, September 27

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2012

“CD47: A multifunctional adhesion molecule that rolls, arrests and transmigrates”, Invited speaker, Molecular Cardiology Research Center, Tufts Medical Center, Tupper Institute. Hosted by Drs. Pilar Alcaide and Richard Karas, January 17

2013 2014 2015 2016 2018

“CD47 as a moderator of the adoptive immune response.” Invited seminar, Pluto Society Meeting, Tortola, British Virgin Islands, March 10-11 “CD47, a key regulator of the innate and adoptive immune responses.” Invited seminar, Department of Pathology and Laboratory Medicine, Temple University, Philadelphia, PA, Hosted by Dr. Yuri Persidsky, June 28-29 “Regulation of T cell trafficking by CD47, a β2 integrin-associated molecule.” FASEB Research Conference, Gastrointestinal Tract XV: Epithelia, Microbes, Inflammation and Cancer, Steamboat Springs, CO. Invited seminar speaker, August 14 “Regulation of T cell trafficking by CD47, a β2 integrin associated molecule” Invited seminar, Dr. Joel Katz, host. The Joint DFCI-BWH Immunology research seminar, Boston, MA. September 30 “Regulation of T cell trafficking by CD47, a β2 integrin associated molecule” Experimental Biology, 2014 Blood Vessel Club Symposium, Session entitled “New Players and Concepts in Leukocyte Trafficking During Inflammation” April 26 ‘New players in leukocyte recruitment: CD47 dependent regulation of integrins functions and beyond”. Invited Speaker. Inflammation Research Symposium, sponsored by Division of Surgical Research, Department of Surgery, Rhode Island Hospital and Brown University, September 25 – 26 PISA Meeting, Pathways to translational medicine; Plenary Session entitled: Recent advances in Cell Injury, Inflammation and Neoplasia, entitled presentation was “CD47 and its expanding role in adaptive immunity” October 6-8

“CD47 and its expanding role in innate and adaptive immune responses” presented at Cardiovascular Medicine Grand Rounds, Jewish Hospital Rudd Heart and Lung Institute, University of Louisville, KY. October 21, Hosted by Dr. Sanjay Srivastava, Ph.D, F.A.H.A.

“CD47 and EMCN: their role in innate and adaptive immune responses” presented at Pathobiology of Disease Seminar Series, Department of Pathology and Medicine, University of Chicago, Chicago IL. February 11, Hosted by Konstantin Birukov, MD, PhD.

“CD47 and Its Expanding Role in Innate and Adaptive Immune Responses” presented at President’s Symposia, The Microcirculation Society at Experimental Biology 2016. Blood cell – Microvessel Interactions Symposia. Invited by Rolano Rumblaut, MD. April 2nd.

“Understanding CD47 – SIRPα Interaction in Adaptive Immune Cell Function”, invited lecture, EB2018 ASIP Symposium: Inflammation, Immunopathology, and Mucosal Disease Mechanisms. EB2018, San Diego, CA. April 22, 2018.

International

1990 “Endothelial-dependent adhesion mechanisms regulate leukocyte adhesion and transmigration across cytokine activated human endothelium”, Invited lecture, Sixth

20

International Symposium on the Biology of the Vascular Endothelial Cell, Paris, France, September 6

1995 “Mechanisms of monocyte adhesion to endothelium under flow in vitro”, Invited Plenary Speaker, Molecular Mechanisms Inflammation Conference, Schloβ Ringberg, München, Germany, September 20

1996 “Mechanisms of CD4+ T Cell adhesion to E- and P-selectin under flow”, Invited plenary lecture, Vascular Endothelium and Regulation of Leukocyte Traffic Conference. Sponsored by The Instituto Juan March de Estudios e Investigaciones. Madrid, Spain

Endothelial-dependent mechanisms of chronic inflammatory leukocyte recruitment,” Invited lecture, Prestige Lecture Series, The Vascular Research Unit, Department of Biomedical Sciences, University of Leeds, Leeds, England

1997 “Endothelial dependent mechanisms of leukocyte adhesion and transmigration in vitro.” Invited seminar. Presented at University of Calgary, Canada, Department of Biology and Biophysics, Calgary, Canada. Hosted by Dr. Paul Kubes, December 11

“Leukocyte endothelial adhesive interactions under flow”, Invited lecture, Centro de Biologia Molecular, Facultad de Ciencias, Universidad Autonoma, Madrid, Spain. Host: Dr. M. Fresno, April 18.

“Leukocyte endothelial adhesive interactions under flow”, Inmulogica ServicoHospital de la Princesa. Madrid, Spain. Hosted by Drs. F. Sanchez-Madrid and M. O de Landzuri, March

“Leukocyte endothelial adhesive interactions under flow”, Inmulogica ServicoHospital de la Princesa, Madrid, Spain. Hosted by Drs. F. Sanchez-Madrid and M. O de Landzuri, March 2

“Neutrophil-endothelial interactions and transmigration, presented at Centro Nacional Dd Biotecnologia, Campus de la Universidad Autonoma, Madrid Spain. Hosted by Dr. Carlos A. Martinez, April 23

1999 "Chemokines IL-8 and MCP-1trigger blood monocyte stable arrest to vascular endothelium under fluid shear in vitro”, Invited speaker, the Fifth Saratoga International Conference on Atherosclerosis, Barcelona, Spain, May 18-21

2000 “Chemokine regulation of T cell adhesion under flow conditions in vitro", Invited Plenary speaker, The World 5th Congress on Trauma, Shock, Inflammation and Sepsis, Munich, Germany. Hosted by Dr. E. Faist, February 29 – March 4

2001 “From start to finish: Leukocyte adhesion to transmigration.” Invited seminar, Department of Laboratory Science and Pathology, University of Toronto, Toronto, Ontario Canada. Hosted by Drs. Myron Cybulsky and Avrium Gotlieb, January 15

“Live-time imaging of vascular endothelial-cadherin (VE-cadherin) during leukocyte transmigration across endothelium under flow” Invited Speaker, Major Session “Flow Modulation of cell-cell interactions”, XVIII Congress of The International Society on Thrombosis and Haemostasis, Paris France, July 11

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2002 “Role of endothelial cell lateral junctions in leukocyte transmigration”, Invited Plenary Research Seminar, Minisymposium “Migration and Immunity”, British Society of Immunology, Harrogate, England, December 5

2003 “Observations of leukocyte transmigration”, Invited plenary speaker, Mminisymposium Transendothelial migration, the 2nd International Workshop on New Therapeutic Targets in Vascular Biology, Geneva, Switzerland, February 7

2004 “Leukocyte transendothelial migration: Lessons from live cell imaging studies”, Invited Plenary, Speaker, Workshop 4, The XIII International Vascular Biology Meeting, Toronto, Ontario Canada, June 5

2008 How signaling and mechanical forces contribute to leukocyte diapedesis”, Invited Seminar Presentation, Workshop, The XIV International Vascular Biology Meeting, Sydney, Australia, June 3

“Biochemical and mechanical signals during leukocyte transmigration”, Invited Plenary Speaker, Leukocyte Trafficking Conference, Schloβ Ringberg, München, Germany, September 3-6

2009 “A new look for CD47 integrin associated protein in T cell recruitment.” Invited speaker, presented at the European Society of Cardiology, Barcelona, Spain, September 2.

2010 “Role of CD47 in the regulation of T cell integrin affinity activation”, Invited Seminar, Pluto Society Meeting, St. Croix, USVI

2012 “CD47 regulation of leukocyte adhesion and recruitment to sites of inflammation”. Invited Seminar, Workshop Session, The XVII International Vascular Biology Meeting, Wiesbaden, Germany, June 3

“CD47 regulation of adoptive immune cell recruitment in vivo”, Invited Seminar, Max Plank Institute, Munster, Germany, Hosted by Professor Dietmar Vestweber, June 5.

2015 2016

Second International Symposia “Cancer and Inflammation: from adherens junctions to disease. Invited seminar entitled “Exploring new models of endothelial cell dependent mechanisms that regulate leukocyte recruitment during inflammation. Universidad Austral de Chile, Valdiva, Chile. January 16-17. “Endothelial adhesion molecules form in cis complexes that signal during leukocyte recruitment” presented at Department of Molecular Cell Biology, Sanquin Research and Landsteiner Laboratory, Academic Medical Center, University of Amsterdam, September 9. Hosted by Drs. Peter Hordijk and Jaap van Buul. “CD47 regulation of leukocyte integrins in immune mediated disease” presented at Schloss Ringberg Symposium entitled Molecular Mechanisms in Leukocyte Trafficking at the Max Plank Institute’s Ringberg Castle, Germany. September 12-17. Organized by Professor Dietmar Vestweber.

“The complicated life of CD47 in innate and adaptive immune responses”, presented at Departament de Biomedicina, University of Barcelona Medical School, Barcelona Spain, June 3. Hosted by Pablo Rocamora Engel, MD.

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2017

“Vascular endothelium: It’s more than just a monolayer”, Keynote Address in Session II entitled Surface Barriers to Inflammation. Tear Film and Ocular Surface Society (TFOS) Annual Meeting, September 9, Montpellier, France.

“Germline deletion of CD47, a “don’t eat me signal”, promotes pro-inflammatory adaptive immunity and atherosclerosis.” Society of Leukocyte Biology Meeting Vancouver, BC, Canada, Oct 5 -8 2017. Invited lecture and Co-Chair, Systems Biology of Adaptive Immune Memory Symposia.

Report of Scholarship

Publications

Peer reviewed publications in print or other media

1. Lionetti FJ, Luscinskas FW, Hunt SM, Valeri CR, Callahan AB. Factors affecting cryogenically

preserved granulocytes. Cryobiology 1980;17:297-310. 2. Aranout MA, Luscinskas FW, Lionetti FJ, Alper CA, Valeri CR. Alternative complement pathway

dependent ingestion of fluolite particles by human granulocytes. J Immunol 1981;127:278-281. 3. Luscinskas FW, Lionetti FJ, Melaragno AJ, Valeri CR. Long term cryopreservation of dog

granulocytes. Cryobiology 1983;20:1-6. 4. Newburger PE, Luscinskas FW, Ryan T, Beard CJ, Wright J, Platt OS, Simons ER, Tauber AI.

Variant chronic granulomatous disease: Modulation of the neutrophil defect by severe infection. Blood 1986;68:914-919.

5. Luscinskas FW, Mark DE, Lionetti FJ, Cragoe EJ, Simons ER. The role of transmembrane

cationic gradients in immune complex stimulation of human neutrophils. J Cell Physiol 1988;134: 211-219.

6. Wheeler ME, Luscinskas FW, Bevilacqua MP, Gimbrone MA, Jr. Human endothelial cells

stimulated with cytokines or endotoxin produce an inhibitor of leukocyte adhesion. J Clin Invest 1988;82:1211-1218.

7. Luscinskas FW, Brock AF, Arnaout MA, Gimbrone MA, Jr. Endothelial-leukocyte adhesion

molecule-1 (ELAM-1)-dependent and leukocyte (CD11/18)-dependent mechanisms contribute to polymorphonuclear leukocyte adhesion to cytokine-activated human vascular endothelium. J Immunol 1989;142:2257-2263.

8. Del Buono BJ, Luscinskas FW, Simons ER. The preparation and characterization of plasma

membrane vesicles from human neutrophils. J Cell Physiol 1989;141:636-644. 9. Luscinskas FW, Nicolaou KC, Webber SE, Veale CA, Gimbrone MA, Jr., Serhan CN. Ca2+

mobilization with leukotriene A4 and epoxytetraenes in human neutrophils. Biochemical Pharm 1990;39:355-365.

10. Nigam S, Fiore S, Luscinskas FW, Serhan CN. Lipoxin A4 and lipoxin B4 stimulate the release

but not the oxygenation of arachidonic acid in human neutrophils: dissociation between lipid

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remodeling and adhesion. J Cell Physiol 1990;143:512-523. 11. Lionetti FJ, Luscinskas FW, Curran TG, Meehan RT, Taylor GR, Carter JH, Jacobson MS,

Surgenor DM. Human blood cells at microgravity: The NASA initial blood storage experiment. Transfusion 1990;30:605-616.

12. Hébert CA, Luscinskas FW, Kiely J-M, Luis EA, Darbonne WC, Bennett GT, Liu C-C, Baker JB,

Obin MS, Gimbrone, MA, Jr. Interactions of neutrophils with two forms of interleukin-8. J Immunol 1990;145:3033-3040.

13. Luscinskas FW, Cybulsky MI, Kiely J-M, Peckins CS, Davis VM, Gimbrone MA, Jr. Cytokine-activated human endothelial monolayers support enhanced neutrophil transmigration via a mechanism involving both Endothelial-leukocyte Adhesion Molecule-1 and Intercellular Adhesion Molecule-1. J Immunol 1991;146:1617-1625.

14. Bochner BS, Luscinskas FW, Gimbrone MA,Jr., Newman W, Sterbinsky SA, Derse-Anthony CP,

Klunk D, Schleimer RP. Adhesion of human basophils, eosinophils and neutrophils to IL-1-activated human endothelial cells: Contributions of endothelial cell adhesion molecules. J Exp Med 1991;173:1553-1556.

15. Spertini O, Luscinskas FW, Kansas GS, Griffin JD, Gimbrone MA, Jr., Tedder TF. Leukocyte

Adhesion Molecule (LAM-1, L-selectin) interacts with an inducible endothelial cell ligand to support leukocyte adhesion. J Immunol 1991;147:2565-2573.

16. Denton MD, Marsden PA, Luscinskas FW, Brenner BM, Brady HR. Cytokine-induced phagocyte

adhesion to human mesangial cells: Role of CD11/CD18 integrins and ICAM-1. Am J Physiol 1991;261:F1071-1079.

17. Shen J, Luscinskas FW, Connolly A, Dewey CF, Gimbrone MA, Jr. Fluid shear stress modulates

cytosolic free Ca2+ in vascular endothelial cells. Am.J.Physiol 1992;262:C384-390. 18. Schleimer RP, Sterbinsly SA, Kaiser J, Bickel CA, Klunk DA, Tomioka K, Newman W,

Luscinskas FW, Gimbrone MA, Jr., McIntyre B, Bockner BS. Interleukin-4 induces adherence of human eosinophils and basophils but not neutrophils to endothelium: association with expression of VCAM-1. J Immunol 1992;148:1086-1092.

19. Spertini O, Luscinskas FW, Gimbrone MA, Jr., Tedder TF. Monocyte attachment to activated

human endothelium in vitro is mediated by LAM-1 (L-selectin) under non-static conditions. J Exp Med 1992;175:1789-1792.

20. Luscinskas FW, Kiely J-M, Ding H, Obin MS, C. Hébert, Baker J, Gimbrone MA,Jr. The in vitro

inhibitory effect of IL-8 and other chemoattractants on neutrophil-endothelial adhesive interactions. J Immunol 1992;149:2163-2170.

21. Shen J, Gimbrone MA , Luscinskas FW, Dewey CF. Regulation of nucleotide concentration at

endothelium-fluid interface by viscous shear flow. Biophy J 1993;64:1323-1330. 22. Ley K, Baker JB, Cybulsky MI, Gimbrone MA, Jr., Luscinskas FW. Intravenous interleukin-8

inhibits granulocyte emigration from rabbit mesenteric venules without altering L-selectin expression or leukocyte rolling. J Immunol 1993;151:6347-6357.

23. Shen J, Luscinskas FW, Gimbrone MA,Jr., Dewey CF, Jr. Fluid flow modulates vascular

24

endothelial cytosolic calcium response to adenine nucleotides. Microcirculation 1994;1:67-68. 24. Pizcueta P, Luscinskas FW. Monoclonal antibody blockade of L-selectin inhibits circulating

neutrophil, lymphocyte and monocyte recruitment to chronic inflammatory sites in vivo. Am J Pathol 1994;145:461-469.

25. Luscinskas FW, Kansas GK, Ding H, Pizcueta P, Boris E. Schleiffenbaum, Tedder TF, Gimbrone MA, Jr. Monocyte rolling, arrest and spreading on IL-4-activated vascular endothelium under defined flow is mediated via sequential action of L-selectin, ß1-integrins and ß2-integrins. J Cell Biol 1994;125:1417-1427.

26. Jiang Y, FW Luscinskas, Graves, D.T. Monocyte chemoattractant protein-1 (MCP-1)

stimulated monocyte attachment to extracellular matrix proteins is mediated by ß2-integrins. Am J Physiol (Cell Physio) 1994;267:C1112-1118.

27. Luscinskas FW, Ding H, Lichtman AH. P-selectin and Vascular cell Adhesion Molecule-1

mediate rolling and arrest, respectively, of CD4+ T lymphocytes on tumor necrosis factor-activated vascular endothelium under flow. J Exp Med 1995;181:1179-1186.

28. Penberthy T, Jiang Y, Luscinskas FW, Graves DT. β2-integrins preferentially mediate

monocyte chemoattractant protein-1 (MCP-1) induced monocyte migration on extracellular matrix proteins. Am J Physiol 1995;269 (Cell Physiol. 38):C60 - 68.

29. Gerritsen ME, Shen CP, McHugh M, Atkinson WJ, Kiely JM, Milstone DS, Luscinskas FW,

Gimbrone MA, Jr. Activation-dependent isolation and culture of murine pulmonary microvascular endothelium. Microcirculation 1995;2:151-163.

30. Read MA, Neish AS, Luscinskas FW, Palombella VJ, Maniatis T, Collins T. The proteasome pathway is required for cytokine-induced endothelial leukocyte adhesion molecule expression. Immunity 1995;2:493-506.

31. Kiely J-M, Cybulsky MI, Luscinskas FW, Gimbrone MA, Jr. Immunoselective trageting of

anti-thrombin agent to the surface of cytokine-activated vascular endothelial cells. Arterioscler Thromb Vasc Biol 1995;15:1211-1218.

32. Pierce JW, Read MA, Ding H, Luscinskas FW, Collins T. Salicylates inhibit IkB- phosphorylation, endothelial leukocyte adhesion molecule expression and neutrophil transmigration. J Immunol 1996;156:3961-3969.

33. Luscinskas FW, Ding H, Tan P, Cumming D, Tedder TF, Gerritsen ME. L-selectin and P-

selectin but not VLA-4 mediate monocyte initial attachment and rolling on TNF-α activated vascular endothelium under flow in vitro. J Immunol 1996;156:326-335.

34. Lichtman AH, Cybulsky MI, Luscinskas FW. Immunology of atherosclerosis: The promise of

mouse models. Am J Pathol 1996;149:351-357. 35. Goetz DJ, Ding H, Cumming D, Luscinskas FW. A colon carcinoma cell line exhibits

adhesive interactions with P-selectin under fluid flow via a PSGL-1 independent mechanism. Am J Pathol 1996;149:1661-1673.

36. Gerszten RE, Luscinskas FW, Ding H, Dichek DA, Stoolman LM, Gimbrone MA, Jr.,

Rosenzweig A. Adhesion of memory lymphocytes to VCAM-1-transduced human vascular endothelial cells under simulated physiological flow conditions in vitro. Circ Res 1996;79:

25

1205-1215. 37. Lichtman AH, Ding H , DesRoches L, Vachino G, Cumming D, Luscinskas FW.

Conversion of CD4+ T lymphocytes from CD45RA+RO- (naive) to CD45RO+ (memory) confers rolling on E- and P-selectin and VCAM-1. J Immunol 1997;158:3640-3650.

38. Allport JR, Ding HT, Ager A, Steeber DA, Tedder TF, Luscinskas FW. L-selectin shedding does not regulate human neutrophil attachment, rolling, or transmigration across human vascular endothelium in vitro. J Immunol 1997;158:4365-4372.

39. Goetz DJ, Greif DM, Ding H, Camphausen RT, Howes S, Comess KM, Snapp KR, Kansas GS,

Luscinskas FW. Isolated P-selectin glycoprotein ligand-1 dynamic adhesion to P- and E-selectin. J Cell Biol 1997;136:509-513.

40. Allport JR, Ding HT, Collins T, Gerritsen ME, Luscinskas FW. Endothelial-dependent

mechanisms regulate leukocyte transmigration: A process involving the proteasome and disruption of the VE-cadherin complex at endothelial cell junctions. J Exp Med 1997;186:517-527.

41. Montoya MC, Luscinskas FW, del Pozo MA, Aragonés, de Landazuri MO. Reduced intracellular

oxidative metabolism promotes firm adhesion of human polymorphonuclear leukocytes to vascular endothelium under flow conditions. Eur J Immunol 1997; 27:1942-1951.

42. Snapp KR, Ding H, Atkins K, Luscinskas FW, Warnke R, Kansas GS. A novel P-selectin

glycoprotein ligand (PSGL-1) monoclonal antibody recognizes an epitope within the tyrosine sulfate motif of human PSGL-1 and blocks recognition of both P- and L-selectin. Blood 1998; 91:154-164.

43. Gerzsten RA, Lim, Y-C, Ding H, Snapp K, Kansas GS, Didchek D, Cabanas C, Sanchez-Madrid F, Rosenzweig A and Luscinskas FW. Adhesion of monocytes to VCAM-1 transduced human endothelial cells: Implications for atherogenesis. Circ Res 1998;82:871-878.

44. Lim Y-C, Ding H, Snapp K, Kansas GS, Luscinskas FW. Contributions of PSGL-1 to monocyte

initial attachment and subsequent accumulation on TNF-α activated endothelium under flow conditions in vitro. J Immunol 1998;161:2501-2508.

45. Tu L, Delahunty MD, Ding H, Luscinskas FW, Tedder TF. The L-selectin ligand induced on

human vascular endothelial cells and expressed by human leukocytes is the cutaneous lymphocyte antigen (CLA). J Exp Med 1999;189:241-252.

46. Lim Y-C, Henault L, Snapp K, Kansas GS, Luscinskas FW, Lichtman AH. Expression of

functional selectin ligands on Helper T cells is differentially regulated by Interleukin-12 and Interleukin-4. J Immunol 1999;162:3193-3201.

47. Gerzsten RA, Garcia-Zepeda E, Yoshida M, Ding H, Gimbrone MA, Luster A, Luscinskas FW,

Rosenzweig A. MCP-1 and IL-8 trigger firm adhesion of monocytes to vascular endothelium under laminar flow conditions. Nature 1999;398:718-723.

48. Montoya MC, Borges E, Snapp KR, Holtmann K, Sánchez-Madrid F, Luscinskas FW, Kansas

GS, Vestweber D, de Landázuri MO. Memory B lymphocytes from secondary lymphoid organs interact with E-selectin through a novel glycoprotein ligand. J Clin Invest 1999;103:1317-1327.

49. Xie H, Lim Y-C, Luscinskas FW, Lichtman AH. Acquisition of selectin binding and peripheral

homing properties by CD4+ and CD8+ T cells. J Exp Med 1999;189:1765-1775.

26

50. Allport JR, Ding H, Muller WA, Luscinskas FW. Monocytes induce reversible focal changes in VE-cadherin complex during transendothelial migration under flow. J Cell Biol. 2000;148:203-216.

51. Canas-Barouch F, Kazuaki M, Allprt JR, Fujita K, Bursell S-E, Aiello LP, Luscinskas FW, Adamis

AP. Integrin-mediated neutrophil adhesion and retinal leukostasis in diabetes. Assoc Res Vis Opth 2000;41:1153-1158.

52. Lim Y-C, Henault L, Goetz DJ, Yednock T, Cabañas C, Sanchéz-Madrid F, Lichtman AH,

Luscinskas FW. α4β1-integrin activation is necessary for high-efficiency T-cell subset interactions with VCAM-1 under flow. Microcirculation 2000;7:201-214.

53. Coxon A, Cullere X, Wakelin M, Luscinskas FW, Mayadas-Norton T. A novel paradigm for neutrophil recruitment by immune complexes under physiologic flow. Immunity 2001;14:693-704.

54. Shaw SK, Bamba, PS, Perkins, BN, Luscinskas FW. Real-time imaging of vascular endothelial-cadherin during leukocyte transmigration across endothelium. J Immunol 2001;167:2323-2330.

55. Shaw SK, Perkins BN, Lim Y-C, Ma S, Liu Y, Nusrat A, Schnell FJ, Parkos CA, Luscinskas FW. Reduced expression of junctional adhesion molecule and platelet/endothelial cell adhesion

molecule-1 (CD31) at human vascular endothelial junctions by cytokines tumor necrosis factor-

plus interferon- does not reduce leukocyte transmigration under flow. Am J Pathol 2001;159: 2281-2291.

56. Lim Y-C, Xie H, Lichtman AH, Luscinskas FW. IL-12, STAT4 dependent upregulation of naive T

cell core 2 β-1,6-N-acetylglucosaminyltransferase (G2GnT), an enzyme essential for expression of functional selectin ligands. J Immunol 2001;167:4476-4484.

57. Boyce JA, Mellor EA, Lim YC, Perkins B, Luscinskas FW. Human mast cell progenitors use 4-integrin -VCAM-1 and PSGL-1 - E-selectin for adhesive interactions with human vascular endothelium under flow conditions. Blood 2002;99:2890-2896.

58. Allport JR, Lim Y-C, Shipley JM, Senior RM, Shapiro SD, Matsuyoshi N, Vestweber D,

Luscinskas FW. Neutrophil Elastase or MMP-9 are not essential for murine neutrophil transendothelial migration in vitro. J Leuk Biol 2002;71:821-28.

59. Grabie N, Delfs MW, Lim Y-C, Westrich J, Luscinskas FW, Lichtman AH. ST3Gal-1

sialyltransferase gene expression during Th2 but not Th1 differentiation: implications for Core-2 glycan formation on cell surface molecules. Eur J Immunol 2002;32:2766-72.

60. Lim YC, Garcia-Cardena G, Allport JR, Zervoglos M, Connolly AJ, Gimbrone Jr. MA,

Luscinskas FW. Heterogeneity of endothelial cells from different organ sites in T-cell subset recruitment. Am J Pathol 2003;162:1591-1601.

61. Ancuta P, Rao R, Moses A, Mehle A, Shaw SK, Luscinskas FW, Gabuzda D. Fractalkine

selectively mediates arrest and migration of CD16+ monocytes. J Exp Med 2003;197:1701-07. 62. Ehlers R, Ustinov V, Chen Z, Zhang X, Rao R, Luscinskas FW, Lopez J, Plow E, Simon D.

Targeting platelet-leukocyte interactions: Identification of the integrin Mac-1 binding site for the

platelet counter-receptor glycoprotein Ibα. J Exp Med 2003;198:1077-1088.

63. Kolesnikova TV, Stipp CS, Rao RM, Lane WS, Luscinskas FW and Hemler MA. EWI-2

modulates lymphocyte integrin α4β1 integrin functions. Blood 2004;103:3013 – 3019.

27

64. Banerjee SS, Lin Z, Atkins GB, Greif DM, Rao RM, Feinberg MW, Chen Z, Simon SI, Luscinskas

FW, TM Michel, Gimbrone MA, Garcia-Cardena G, Jain MK. Identification of KLF-2 as a novel transcriptional regulator of endothelial proinflammatory activation. J Exp Med 2004;199:1304-1315.

65. Rao R, Betz T, Lamont D, Kim MB, Shaw SK, Froio R, Baleux F, Arenzana-Seisdedos F, Alon R,

Luscinskas FW. Elastase release by transmigrating neutrophils deactivates endothelial bound SDF-1 and attenuates subsequent T lymphocyte transendothelial migration. J Exp Med 2004; 200:713-724.

66. Shaw SK, Ma S, Kim MB, Rao RM, Hartman CU, Froio R, Yang L, Jones T, Liu Y, Nusrat A,

Parkos CA, Luscinskas FW. Coordinated redistribution of leukocyte LFA-1 and endothelial ICAM-1 accompany neutrophil transmigration. J Exp Med 2004;200:1571-1580.

67. Cullere X, Shaw SK, Andersson L, Luscinskas FW, Mayadas TN. Regulation of vascular

endothelial barrier function by Epac, a cAMP activated exchange factor for Rap GTPase. Blood 2005;105:1950-5.

68. Yang L, Froio RM, Sciuto TE, Dvorak AM, Alon R and Luscinskas FW. ICAM-1 regulates

neutrophil adhesion and transcellular migration of TNF- activated vascular endothelium under flow. Blood 2005;106:584-92.

69. Lord GM, Rao RM, Choe H, Sullivan BM, Lichtman AH, Luscinskas FW, Glimcher LH. T-bet is

required for optimal pro-inflammatory CD4+ T cell trafficking. Blood 2005;106:3432-3439. 70. Kawakami A, Aikawa M, Alcaide P, Luscinskas FW, Libby P, Sacks FM. Apolipoprotein CIII in

apoB lipoproteins enhances the adhesion of human monocytic cells to endothelial cells. Circulation 2006;113:691-700.

71. Yang L, Kowalski JR, Zhan X, Thomas SM, Luscinskas FW. Endothelial cell cortactin phosphorylation by Src contributes to polymorphonuclear leukocyte transmigration in vitro. Circ Res 2006;98:394-402.

72. Xie C, Alcaide P, Schneider D, Herrmann M, Preissner KT, Luscinskas FW, Chavakis T. Suppression of experimental autoimmune encephalomyelitis by extracellular adherence protein (Eap) of Staphylococcus aureus. J Exp Med 2006;203:985-994.

73. Cai YH, Alvarez A, Alcaide P, Duramad P, Lim YC, Jarolim P, Lowe JB, Luscinskas FW,

Lichtman AH. Abrogation of functional selectin-ligand expression reduces migration of pathogenic CD8+ T cells into heart. J Immunol 2006;176:6568-75.

74. Harari OA, Alcaide P, Ahl D, Luscinskas FW, Liao JK. 2006. Absence of TRAM restricts Toll-like

receptor 4 signaling in vascular endothelial cells to the MyD88 pathway. Circ Res 2006;98:1134-40.

75. Lim YC, Luscinskas FW. Isolation and culture of murine heart and lung endothelial cells for in vitro model systems. Methods Mol Biol 2006;341:141-54.

76. Kawakami A, Aikawa M, Alcaide P, Luscinskas FW, Libby P, Sacks FM. Apolipoprotein CIII

induces expression of vascular cell adhesion molecule-1 in vascular endothelial cells and increases adhesion of monocytic cells. Circulation 2006;114:681-687.

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77. Yang L, Kowalski JR, Yacono P, Bajmoczi M, Shaw SK, Froio RM, Golan DM, Thomas SM and Luscinskas FW. 2006. Endothelial cell cortactin coordinates ICAM-1 clustering and actin cytoskeleton remodeling during polymorphonuclear leukocyte adhesion and transmigration. J Immunol 2006;177:6440-6469.

78. Swirski FK, Aikawa E., Alcaide P, Luscinskas FW, Libby P, Weissleder R, Pittet MJ. Ly-6Chi

monocytes dominate atherosclerosis-associated monocytosis and give rise to lesional macrophages. J Clin Invest 2007;117:195-205.

79. Lou O, Alcaide P, Luscinskas FW, Muller WA. CD99 is a key mediator of the transendothelial

migration of neutrophils. J Immunol 2007;178:1136-1143. 80. Alcaide P, Tatiana G, Jones TG, Lord GM, Glimcher LH, Xu Y, Gurish MA, Luscinskas FW.

Dendritic cell expression of T-bet regulates mast cell progenitor homing to mucosal tissues. J Exp Med 2007;204:431-439.

81. Alcaide P, King SL, Dimitroff CJ, Lim Y-C, Fuhlbrigge RC, Luscinskas FW. The 130-kDa glycoform of CD43 functions as an E-selectin ligand for activated Th1 cells in vitro and in delayed-type hypersensitivity reactions in vivo. J Invest Derm 2007;127:1964-1972.

82. Sircar M, Bradfield P, Aurrand-Lions MC, Fish RJ, Alcaide P, Yang L, Newton G, Lamont D,

Sehrawat S, Mayadas T, Liang TW, Parkos CA, Imhof BA, Luscinskas FW. Neutrophil transmigration under shear flow conditions in vitro is JAM-C independent. J Immunol 2007; 178:5879-5887.

83. Bradfield PF, Scheiermann C, Nourshargh S, Ody C, Luscinskas FW, Rainger GE, Nash GB, Aurrand-Lions M, Imhof BA. JAM-C regulates unidirectional monocyte transendothelial migration in inflammation. Blood 2007;110:2545-2555.

84. Ghandour H, Cullere X, Luscinskas FW, Mayadas TN. Essential role for Rap1 GTPase and its guanine exchange factor CalDAG-GEFI in LFA-1 but not VLA-4 integrin mediated human T-cell adhesion. Blood 2007;110:3682-3690.

85. Rabodzey A, Yao Y, Luscinskas FW, Shaw SK, Dewey DF Jr. Early response of endothelial cells to flow is mediated by VE-cadherin. Cell Commun Adhes 2007;14:195-209.

86. Noma K, Rikitake Y, Oyama N, Yan G, Alcaide P, Liu P, Wang H, Ahl D, Sawada N, Okamoto R, Hiroi Y, Shimizu K, Luscinskas FW, Sun J, Liao J. ROCK1 mediates leukocyte recruitment and neointima formation following vascular injury. J Clin Invest 2008;118:1632-44. PMCID: PMC 2293333

87. Rabodzey A, Alcaide P, Luscinskas FW, Benoit Ladoux B. Mechanical forces induced by the transendothelial migration of human neutrophils. Biophys J 2008;95:1428-38. PMCID: PMC 2479581

88. Stefanidakis M, Newton G, Lee WY, Parkos CA, Luscinskas FW. Endothelial CD47 interaction

with SIRP is required for human T cell transendothelial migration under shear flow conditions in vitro. Blood 2008;112:1280-89. PMCID: PMC2515120.

89. Alcaide P, Newton G, Sehrawat S, Mayadas-Norton T, Vincent P, Yacono P, Golan DE, Kowalcyk

A. and Luscinskas FW. p120-catenin regulates leukocyte transmigration through an effect on VE-Cadherin phosphorylation. Blood 2008;112:2770-79. PMCID: PMC2556612

90. Yano K, Okada Y, Beldi G, Shih SC, Bodyak N, Okada H, Kang PM, Luscinskas FW, Robson

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SC, Carmeliet P, Karumanchi SA, Aird WC. Elevated levels of placental growth factor represent an adaptive host response in sepsis. J Exp Med 2008;205:2623-31.

91. Kasorn A, Alcaide P, Yonghui, J. Subramanian, KK, Sarraj B., Li Y, Loison F, Hattori H,

Silberstein, L. Luscinskas FW, Luo HR. Focal adhesion kinase (FAK) regulates pathogen-killing capability and life-span of neutrophils via mediating both adhesion-dependent and -independent cellular signals. J Immunol 2009;183(2):1032-43. Epub 2009 Jun 26.

92. Alcaide P, Lim YC, Luscinskas FW, Fresno M. Mucin AgC10 from Trypanosoma cruzi Interferes with L-selectin-mediated monocyte adhesion. Infect Immun. 2010;78:1260-8. Epub 2010 Jan 11.

93. Azcutia V, Abu-Taha M, Romacho T, Vázquez-Bella M, Matesanz N, Luscinskas FW,

Rodríguez-Mañas L, Sanz MJ, Sánchez-Ferrer CF, Peiró C. Inflammation determines the pro-adhesive properties of high extracellular d-glucose in human endothelial cells in vitro and rat microvessels in vivo. PLoS One 2010;5(4):e10091.

94. Koduru S, Kumar L, Massaad MJ, Ramesh N, Le Bras S, Ozcan E, Oyoshi MK, Kaku M, Fujiwara

Y, Kremer L, King S, Fuhlbrigge R, Rodig S, Sage P, Carman C, Alcaide P, Luscinskas FW, Geha RS. Cdc42 interacting protein 4 (CIP4) is essential for integrin-dependent T-cell trafficking. Proc Natl Acad Sci U S A 2010;107(37):16252-6. Epub 2010 Aug 30.

95. Zhang J, Alcaide P, Liu L, Sun J, He A, Luscinskas FW, Shi GP. Regulation of endothelial cell adhesion molecule expression by mast cells, macrophages, and neutrophils. PLoS One 2011;6(1):e14525.

96. Maganto-García E, Bu DX, Tarrio ML, Alcaide P, Newton G, Griffin GK, Croce KJ, Luscinskas FW, Lichtman AH, Grabie N. Foxp3+-inducible regulatory T cells suppress endothelial activation and leukocyte recruitment. J Immunol 2011;187(7):3521-9. Epub 2011 Aug 26. PMCID: PMC3217244

97. Alcaide P, Maganto-Garcia E, Newton G, Travers R, Croce KJ, Bu DX, Luscinskas FW, Lichtman AH. Difference in Th1 and th17 lymphocyte adhesion to endothelium. J Immunol 2012; 188(3):1421-30. Epub 2012 Jan 4. PMCID: PMC3262911

98. Griffin GK, Newton G, Tarrio M, Bu D, Maganto Garcia E, Azcutia V, Grabie N, Luscinskas FW,

Croce K, Lichtman AH. IL-17 and TNFα Sustain Neutrophil Recruitment During Inflammation Through Synergistic Effects on Endothelial Activation. J. Immunol. 2012;188(12):6287-99. Epub 2012 May 7.

99. Alcaide P, Martinelli R, Newton G, Williams MR, Adam A, Vincent PA, Luscinskas FW. p120-

Catenin prevents neutrophil transmigration independently of RhoA inhibition by impairing Src dependent VE-cadherin phosphorylation. Am J Physiol Cell Physiol 2012;303(4):C385-95. Epub 2012 May 30.

100. Azcutia V, Stefanidakis M, Tsuboi N, Mayadas T, Fukuda D, Aikawa M, Newton G and Luscinskas FW. Endothelial CD47 promotes Vascular Endothelial-cadherin tyrosine phosphorylation and participates in T-cell recruitment at sites of inflammation in vivo. J. Immunol 2012, 189:2553-62. Epub 2012 Jul 18.

101. Zheng C, Azcutia V, Aikawa E, Figueiredo JL, Croce K, Sonoki H, Sacks FM, Luscinskas FW,

Aikawa M. Statins suppress apolipoprotein CIII-induced vascular endothelial cell activation and monocyte adhesion. Eur Heart J 2013 34(8):615-24. [Epub 2012 Aug 26] PMCID: PMC3578265

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102. van Driel B, Liao G, Romero X, O'Keeffe MS, Wang G, Faubion WA, Berger SB, Magelky EM,

Manocha M, Azcutia V, Grisham M, Luscinskas FW, Mizoguchi E, de Waal Malefyt R, Reinecker HC, Bhan AK, Wang N, Terhorst C. Signaling lymphocyte activation molecule regulates development of colitis in mice. Gastroenterology 2012;143(6):1544-1554.e7 [Epub ahead of print].

103. Pasula S, Cai X, Dong Y, Messa M, McManus J, Chang B, Liu X, Zhu H, Mansat RS, Yoon SJ, Hahn S, Keeling J, Saunders D, Ko G, Knight J, Newton G, Luscinskas FW, Sun X, Towner R, Lupu F, Xia L, Cremona O, De Camilli P, Min W, Chen H. Endothelial epsin deficiency decreases tumor growth by enhancing VEGF signaling. J Clin Invest 2012;122:4424-38. Epub 2012 Nov 26.

104. Venkatesh D, Ernandez T, Rosetti F, Batal I, Cullere X, Luscinskas FW, Zhang Y, Stavrakis G, García-Cardeña G, Horwitz BH, Mayadas TN. Endothelial TNF receptor 2 induces IRF1 transcription factor-dependent interferon-β autocrine signaling to promote monocyte recruitment. Immunity 2013;38(5):1025-1037. Epub 2013 Apr 25.

105. Azcutia V, Routledge M, Williams MR, Newton G, Frazier WA, Manica A, Croce KJ, Parkos CA, Schmider AB, Turman MV, Soberman RJ, Luscinskas FW. CD47 plays a critical role in T-cell recruitment by regulation of LFA-1 and VLA-4 integrin adhesive functions. Mol Biol Cell. 2013 Nov;24(21):3358-68. Epub 2013 Sep 4.

106. Martinelli R, Newton G, Carman CV, Greenwood J, Luscinskas FW. Novel Role of CD47 in Rat Microvascular Endothelium: Signaling and Regulation of T Cell Transendothelial Migration. Arterioscler Thromb Vasc Biol. 2013 Aug 29. [Epub ahead of print].

107. Adams WJ, Zhang Y, Cloutier J, Kuchimanchi P, Newton G, Sehrawat S, Aird WC, Mayadas TN, Luscinskas FW, García-Cardeña G. Functional vascular endothelium derived from human induced pluripotent stem cells. Stem Cell Reports. 2013 Jul 25;1(2):105-13.

108. Brown JD, Lin CY, Duan Q, Griffin, G, Paranal RM, Bair S, Newton G, Lichtman AH, Kung K, Yang Y, Wang H, Luscinskas FW, Croce K, Bradner JE, Plutzky J. NF-κB Directs Dynamic Proinflammatory Super Enhancer Formation in Endothelium. Mol Cell. 2014 Oct 23;56(2):219-31. 2014 Sept 24 [Epub ahead of print ]. PMCID: PMC4224636

109. Massaad MJ, Michiko K, Oyoshi MK, Kane J, Koduru S, Alcaide P, Nakamura F, Ramesh N, Luscinskas FW, Hartwig J and Geha RS. Binding of WIP to Actin is Essential for T Cell 1 Actin Cytoskeleton Integrity and Tissue Homing. Mol Cell Biol. 2014 Dec 1;34(23):4343-54. [Sep 22 Epub ahead of print]. PMCID: PMC4248745

110. Rosetti F, Chen Y, Sen M, Thayer E, Azcutia V, Herter JM, Luscinskas FW, Cullere X, Zhu C, Mayadas TN. A lupus-associated variant of Mac-1 (ITGAM) exhibits defects in mechanical force-induced integrin allostery and interaction with ligands. Cell Rep. 2015 Mar 11. pii: S2211-1247(15)00183-72015. PMCID: PMC4567551 available after 10/2016.

111. Wang G, van Driel BJ, Liao G, O'Keeffe MS, Halibozek PJ, Flipse J, Yigit B, Azcutia V, Luscinskas FW, Wang N, Terhorst C. Migration of myeloid cells during inflammation is differentially regulated by the cell surface receptors Slamf1 and Slamf8. PLoS One. 2015 Mar 23;10(3):e0121968. PMCID: PMC4370648

112. Herter J, Grabie N, Cullere X, Azcutia V, Rosetti F, Bennett P, Herter-Sprie G, Elyaman W, Luscinskas FW, Lichtman AH, and Mayadas T. AKAP9 regulates activation-induced T lymphocyte retention at sites of inflammation. Nat Commun. 2015 Dec 18;6:10182. doi:

31

10.1038/ncomms10182. PMCID: PMC4703868

113. Zahr A, Alcaide P, Yang J, Jones A, Gregory M, Patel-Hett SR, Luscinskas FW, Saint-Geniez M, Ksander B, D’Amore PA, Argüeso P. Endomucin prevents leukocyte-endothelial cell adhesion and has a critical role under resting and inflammatory conditions. Nat Commun. 2016 Feb 2;7:10363. doi: 10.1038/ncomms10363. PMCID: PMC4740757

114. Janssen E, Hedayat M, Leick M, Tohme M, Kumari S, Ramesh N, Massaad M, Ullas S, Azcuti V, Goodnow C, Randall K, Qaio Q, Wu H, Al-Herz W, Cox D, Hartwig J, Irvine D, FW Luscinskas, and Geha RS. A DOCK8-WIP-WASP complex links the TCR to the actin cytoskeleton. J Clin Invest. 2016 Oct 3;126 (10):3837-3851. Epub 2016 Sep 6. PMC5096816 [Available on 2017-01-03]

115. Azcutia V, Bassil R, Herter J, Newton G, Mayadas T, Khoury SJ, Parkos CA, Elyaman E, and Luscinskas FW. Defects in CD4+ T cell LFA-1 integrin dependent adhesion and proliferation protect CD47-/- mice in EAE. J. Leuk. Biol. Epub ahead of print December 13, 2016. doi:10.1189/jlb.3A1215-546RR. PMCID: PMC6608006

116. Miyabe Y, Miyabe C, Murooka TT, Kim EY, Newton GA, Kim ND, Haribabu B, Luscinskas FW,

Mempel TR, and Luster AD.Complement C5a Receptor is the Key Initiator of Neutrophil Adhesion Igniting Immune Complex-induced Arthritis. Sci Immunol. 2017 Jan; 2(7): eaaj2195. Published online 2017 Jan 20. doi: 10.1126/sciimmunol.aaj2195. PMCID: PMC5436313.

117. Folco E, Mawson T, Vromman A, Bernardes-Souza B, Franck G, Persson O, Nakamura M, Newton G, Luscinskas FW, and Libby P. Neutrophil Extracellular Traps Induce Endothelial Cell Activation and Tissue Factor Production through Interleukin-1α and Cathepsin G. Arterioscler Thromb Vasc Biol. 2018 Aug;38(8):1901-1912. doi: 10.1161/ATVBAHA.118.311150. PMCID: PMC6202190

118. Engelbertsen D, Autio A, Verwilligen RAF, Depuydt MAC, Newton G, Rattik S, Levinsohn E, Saggu G, Jarolim P, Lichtman AH, Luscinskas FW. Increased lymphocyte activation and atherosclerosis in CD47-deficient mice. Published online July 23, 2019. Sci Rep. 2019 Jul 23;9(1):10608. PMCID: PMC6650435

119. Jain S, Van Scoyk A, Morgan EA, Matthews A, Stevenson K, Newton G, Powers F, Autio A, Louissaint A Jr, Pontini G, Aster JC, Luscinskas FW, Weinstock DM. Targeted Inhibition of CD47-SIRPα Requires Fc-FcR Interactions to Maximize Activity in T-cell Lymphomas. Blood. 2019 Aug 5. doi: 10.1182/blood.2019001744. [Epub ahead of print] PMID:31383641

120. Erin Janssen E, Tohme M, Butts J, Giguere S, Sage PT, Velázquez FE, Kam C, Milin E, Sobh A, Al-Tamemi S, Luscinskas FW, Batista F, Geha RS. 2020 DOCK8 is essential for LFA-1 dependent positioning of T follicular helper cells in germinal centers. Under review at JCI.

Non-peer reviewed scientific or medical publications/materials in print or other media

1. Kansas GS, Spertini O, Luscinskas FW, Gimbrone MA, Jr., Tedder TF. Interaction of LAM-1 with

an Inducible ligand supports leukocyte adhesion to endothelium. In: Lipsky PF, Rothlein R, Kishimoto TK, Faanes RB, Smith CW Eds. Structure, function and regulation of molecules involved in leukocyte adhesion. New York, NY: Springer-Verlag; 1993. p. 168-181.

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2. Tedder TF, Luscinskas FW and Kansas GS. Regulation of leukocyte migration by L-selectin: Mechanisms, domains, and ligands. In: Sorg C, Hogg N, Ruiter D, Eds. The Vascular Endothelium in Inflammation. Behring-Institut-Metteilugen; 1993. Vol 92; p 165-177.

3. Saunder K, Luscinskas FW, Tedder TF. Investigation of a role of CD34, a sialomucin expressed

by human vascular endothelial cells, in L-selectin-mediated adhesion. In: Schlossman SF, Boumsell L, Gilks W, Harlan JM, Kishimoto T, Morimoto C, Ritz J, Shaw S, Silverstein RL, Springer TS, Tedder TF, Todd RF, Eds. Leukocyte Typing Workshop V: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1994. p. 1520-21.

4. Luscinskas FW, Lawler J. Integrins as dynamic regulators of vascular function. FASEB J 1994;

8:929-938. 5. Luscinskas FW, Gimbrone MA Jr. Endothelial-dependent mechanisms in chronic inflammatory

leukocyte recruitment. Ann Rev Med 1996;47:413-423. 6. Tan P, Homer-Vanniasinkam S, Gimbrone,Jr, MA and Luscinskas FW. The role of chemo-

attractants in neutrophil recruitment to sites of inflammation. In: Paul LC, Issekutz TH, Eds. Targeting Cell Adhesion Molecules for Therapeutic Application; 1997. Chapter 10, p. 257-288.

7. Luscinskas, FW. The endothelium in inflammation. In: Bochner BS, Ed. Adhesion molecules in

allergic disease. New York, NY: Marcel Dekker, Inc.; 1997. Chapter 2, p. 21-36. 8. Goetz DJ, Greif D, Shen J, Luscinskas FW. Adhesion of leukocytes under flow. In: Dejana E,

Ed. Adhesion Protein Protocols, Methods in Molecular Biology. Totowa, NJ: Humana Press, Inc.; 1998. p 137-146.

9. Kiely J-M, Luscinskas FW, Gimbrone MA Jr. Leukocyte-endothelial monolayer adhesion assay.

In: Dejana E, Ed. Adhesion Protein Protocols, Methods in Molecular Biology. Totowa, NJ: Humana Press, Inc.; 1998. Vol 96;p 131-136.

10. Tan P, Luscinskas FW, Homer-Vanniasinkam S. Cellular and molecular mechanisms of

inflammation and thrombosis. Eur J Vasc Endovasc Surg 1999;17:373-380. 11. Allport JR, G Garcia-Cardena, Y-C Lim, Luscinskas FW. Current in vitro methods of cell

migration. In: Donnelly L, Ed. Methods in Molecular Biology. Totowa, NJ: Humana Press, Inc.; 2000.

12. Luscinskas FW. Cytokines and endothelial cell activation. In: Ley K, Ed. Physiology of

Inflammation. New York, NY: Oxford University Press; 2001. Ch 5, p. 80-89. 13. Luscinskas FW, Lim Y-C, Lichtman AH. Chemokine driven T cell transmigration - is flow the

missing step? Nature Immunol 2001;2:478-480.

14. Luscinskas FW, Shaw SK. The biology of endothelial cell-cell lateral junctions. Microcirculation

2001;8:141-142. 15. Luscinskas FW, Ma S, Nusrat A, Parkos CA, Shaw SK. Leukocyte transendothelial migration: A

junctional affair. Seminars in Immunol 2002;14:105-113. 16. Luscinskas FW, Ma S, Nusrat A, Parkos CA, Shaw SK. The role of endothelial cell junctions

during leukocyte transendothelial migration. Immunol Rev 2002;186:57-67.

33

17. Liu Y, Shaw SK, Ma S, Yang L, Luscinskas FW, Parkos CA. Regulation of leukocyte

transmigration: cell surface interactions and signaling events. J Immunol 2004;172: 7-14. 18. Alon R, and Luscinskas FW. Crawling and integrating apical cues. Nat Immunol 2004;5:351-353. 19. Alon R, Cinamon G, Luscinskas FW. Real time in vitro assays for studying seukocyte

transendothelial migration under physiological flow conditions. In: Hamann A, Engelhardt B, Eds. Leukocyte Trafficking: Molecular Mechanisms, Therapeutic Targets, and Methods. Wiley-VCH VerlagGmbH & Co. Weinheim, Germany 2005, Chapter 20, pp 437-454.

20. Luscinskas FW, Mayadas T. FcRs join the cascade. Blood 2007;109:3615. 21. Rao RM, Yang L, Garcia-Cardena G, and Luscinskas FW. Endothelial dependent mechanisms

of leukocyte recruitment to the vascular wall. Circ Res 2007;101:234-47. 22. Muller WA, Luscinskas FW. Assays of transendothelial migration in vitro. Methods Enzymol

2008;443:155-76. PMCID: PMC2759874

23. Alcaide P, Auerbach S, Luscinskas FW. Neutrophil recruitment under shear flow: It’s all about endothelial cell rings and gaps. Microcirculation 2009;16:43-57.

24. Luscinskas FW. Neutrophil CD44 rafts and rolls. Blood 2010;116(3):314-5.

25. Williams MR, Luscinskas FW. Leukocyte rolling and adhesion via ICAM-1 signals to endothelial

permeability. Focus on "Leukocyte rolling and adhesion both contribute to regulation of microvascular permeability to albumin via ligation of ICAM-1". Am J Physiol Cell Physiol 2011;301(4):C777-9. Epub Jul 20. PMCID: PMC3191568

26. Williams MR, Azcutia V, Newton G, Alcaide P, Luscinskas FW. Emerging mechanisms of neutrophil recruitment across endothelium. Trends Immunol. 2011;32(10):461-9. Epub 2011 Aug 11. PMCID: PMC3185121

27. McEver RP and Luscinskas FW. Cell Adhesion. Chapter 12, pgs 127 -134. In Hematology, Basic Principals and Practice. 6th Ed. Elsevier and Saunders, 2012.

28. Luscinskas FW. FAK and PAX-illin get involved in leukocyte diapedesis. Eur J Immunol 2012; 42(2):296-8.

29. Luscinskas FW. CXCL1 excess stops neutrophils in their tracks. Blood. 2013; 122(23):3708-10.

30. Luscinskas FW. Neutrophil recruitment: perivascular macrophages 'duke it out' with Staphylococcus aureus. Nat Immunol. 2014; January 15(1):10-11.

31. Luscinskas FW, Imhof BA. Introduction for the special issue on new paradigms in leukocyte trafficking, lessons for therapeutics. Semin Immunopathol. 2014 Mar;36(2):133-6. Epub 2014 Apr 9.

32. Leick M, Azcutia V, Newton G, Luscinskas FW. Leukocyte recruitment in inflammation: basic concepts and new mechanistic insights based on new models and microscopic imaging technologies. Cell Tissue Res. 2015 February 23. [Epub ahead of print]

33. Luscinskas FW, Leick M, Newton G, Nusrat A. Introduction for the special issue on "Tissue

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Barriers in Inflammation". Tissue Barriers. 2015 Feb 25;3(1-2):e1015825. PMCID: PMC4389789

34. McEver RP and Luscinskas FW. Cell Adhesion. Chapter 10, pgs 97 -104. In Hematology, Basic Principals and Practice. 7th Ed. Elsevier and Saunders, 2018.

Professional educational materials or reports, in print or other media

Abstracts (recent only, most published as complete papers), Poster Presentations and Exhibits

Presented at Professional Meetings

1. Alcaide P, Maganto-Garcia E, Newton G, Travers R, Croce K, Bu DX, Luscinskas FW, Lichtman AH. Difference in Th1 and TH17 lymphocyte adhesion to endothelium. Exp Biol Sessions, Washington, DC, 2011.

2. Azcutia-Criado V, Alcaide P, Newton G, Grodecki A, Luscinskas FW. Integrin-associated

protein-CD47 regulates LFA-1 and VLA-4 during leukocyte recruitment. 116.3, Exp Biol Sessions, Washington, DC, 2011.

3. Azcutia-Criado V, Alcaide P, Newton G, Grodecki A, Luscinskas FW. Integrin-associated

protein-CD47 regulates LFA-1 and VLA-4 during leukocyte recruitment. S120, Exp Biol Sessions, Washington, DC, 2011.

4. Adams WJ, Zhang Y, Clouthier J, Kuchimanchi P, Newton G, Sehrawat S, Mayadas T,

Luscinskas FW, Garcia-Cardena G. Generation and specification of functional human vascular endothelium derived from induced pluripotent stem cells. American Heart Association Scientific Sessions, Orlando, FL, 2011.

5. Griffin G, Newton G, Tarrio M, Bu D, Maganto-Garcia E, Grabie N, Luscinskas FW, Croce KJ,

Lichtman AH. Interleukin 17 promotes endothelial activation and neutrophil recruitment through synergistic action with tumor necrosis factor alpha. Arteriosclerosis, Thrombosis, Vascular Biology Meeting 2012, Chicago, IL.

6. Marchini JF, Manica A, Griffin G, Quillard T, Dutzmann J, Travers R, Azcutia-Criado V,

Tesmenitsky Y, Luscinskas FW, Libby P, Stamler JS, Simon DI, Croce K. S-nitroso-glutathione reductase (GSNOR) reduces vascular inflammation and atherosclerosis. Arteriosclerosis, Thrombosis, Vascular Biology Meeting, 2012, Chicago, IL.

7. Adams WJ, Zhang Y, Cloutier J, Kuchimanchi P, Newton G, Sehrawat S, Mayadas TN,

Luscinskas FW, Garcia-Cardena G. Functional human vascular endothelium for personalized cell based assays. Arteriosclerosis, Thrombosis, Vascular Biology Meeting 2012, Chicago, IL.

8. Azcutia V, Routledge, M, Newton G, Manica A, Croce K, Luscinskas FW. Leukocyte CD47

plays a key role in T cell recruitment through affinity regulation of LFA-1 and VLA-4 integrins. 55.2, Exp Biol. Sessions, San Diego, CA, 2012.

9. Azcutia V, Routledge M, Newton G, Manica A, Tarrio M, Croce K, Lichtman AH, Luscinskas FW.

Leukocyte CD47 regulation of LFA-1 and VLA-4 affinity plays a critical role in T-cell recruitment in vivo. Keystone Symposia on Molecular and Cellular Biology: Chemokines and Leukocyte Trafficking in Homeostasis and Inflammation. 8-13 January, Breckenridge, Colorado, 2012

10. Alcaide P, Maganto-Garcia E, Newton G, Travers R, Croce K, Bu DX, Luscinskas FW, Lichtman

AH. Th17 lymphocyte adhesion to endothelium is highly dependent on E-selectin and ICAM-1

35

mediated interactions. 136.1, Exp Biol Sessions, San Diego, CA, 2012. 11. Azcutia V, Bassil R, Khoury S.J, Elyaman W, Luscinskas FW. CD47 reduces activation-induced

CD4+ T cell death in MOG peptide-dependent Experimental Autoimmune Encephalomyelitis. Experimental Biology Meeting 2013. 20-24 April 2013. Boston, MA. Oral presentation.

12. Azcutia V, Routledge M, Williams MR, Newton G, Frazier W, Manica A, Croce K, Parkos CA, Bair

AM, Soberman RJ, Luscinskas FW. CD47 plays a critical role in T cell recruitment by regulation of LFA-1 and VLA-4 integrin adhesive functions. Vasculata 2013, La Jolla, CA. Panel.

13. Azcutia V, Croce K, Herter JM, Newton G, Mayadas TN, Luscinskas FW. CD47 contributes to

neutrophil recruitment. Experimental Biology Meeting 2014, 25-30 April 2014, San Diego, CA. Oral presentation.

14. Veronica Azcutia, Anny-Claude Luissint, Sven Flemming, Miguel Quiros, Asma Nusrat, Francis W.

Luscinskas, Charles A. Parkos. CD47 regulates CD11b-dependent neutrophil transepithelial migration during mucosal intestinal inflammation. Experimental Biology Meeting 2017, April 2017, Chicago, IL. Oral presentation.

15. Anu Autio, Frankie Velazquez Planas, Gail Newton, Manuel Leyva Castillo, Charles A. Parkos,

Pablo Engel and Francis W. Luscinskas. Impaired CD47–SIRPα Interactions in Antigen-Priming Results in Defects in Proliferation In Vivo. FASEB J 33 (1):34.11, 2019.

Narrative Report (limit to 500 words)

Major Research Interests: 1. Cellular physiology and pathophysiology of endothelium 2. Adhesive mechanisms involved in leukocyte-endothelial adhesion and extravasation in

inflammation 3. Role of CD47- SIRPα pathway in innate and adaptive immune system homeostasis in disease

models of multiple sclerosis (EAE, experimental autoimmune encephalomyelitis), atherosclerosis, and hematological and solid tumor malignancies (T cell lymphomas).

Summary of Research Activities: The goal of my research is to understand the mechanisms of leukocyte recruitment in the context of the innate and adoptive immune systems. As a postdoctoral fellow in Dr. Michael A. Gimbrone’s lab at BWH/HMS, we created an in vitro model system that mimicked blood flow in postcapillary venules of the microcirculation. This model enabled evaluation of effects of laminar shear on endothelial cells (refs. 17, 21 and 23). Upon establishing my independent laboratory, we focused identifying molecular mechanisms responsible for adhesion of blood leukocytes to endothelium. Collectively, these studies elucidated the adhesion mechanisms supporting interactions of blood leukocytes with vascular endothelium under physiological shear flow in vitro (refs. 25,27,37,39,40,44-48,53,57), and determined how endothelial or leukocyte derived chemokines influence leukocyte adhesion and transmigration (refs. 43,47). In early 2000, we postulated that leukocytes and endothelial cells undergo rapid and complex morphological and cytoskeletal rearrangements during transendothelial migration. We set out to determine the spatial and temporal behavior of individual adhesion molecules and their intracellular binding partners, and whether this involved linkage with the cytoskeletal apparatus, would provide clues

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to understanding how individual steps such as leukocyte adhesion and transmigration were regulated. Using an in vitro live-cell, fluorescence imaging system and an adenovirus and later lentivirus transduction approaches to deliver GFP-tagged fusion proteins to endothelium, we reported leukocytes transmigrate at both endothelial cell-cell junctions and through the endothelium (ref 68). We discovered that some, but not all junctional proteins, transiently move laterally in the endothelial plasma membrane, out of the path of the transmigrating leukocyte (refs. 54,68,73,78,82). Experimental approaches that locked VE-cadherin at cell borders inhibit leukocyte transmigration by preventing Src kinase-dependent tyrosine phosphorylation of the cytoplasmic tail in VE-cadherin (ref 89,99). In contrast to VE-cadherin, ICAM-1, VCAM-1 and CD47, formed a “ring” surrounding the transmigrating leukocyte (refs. 66,68,76,106). Next, we explored how leukocyte ligation of ICAM-1 and CD47 and other adhesion molecules influences VE-cadherin association with its cytosolic binding partners and tyrosine kinases and tyrosine phosphatases as it relates to transmigration, and more distal events, such as EC gene regulation (refs 106,108). Our recent work investigates how a broadly expressed surface molecule, CD47, also called Integrin

Associated Protein, IAP, and its ligands, Signal Regulatory Protein (SIRPα, CD172a) and SIRP regulate homeostasis of the innate and adaptive immune systems. CD47 also interacts in the same plasma membrane (in cis) with leukocyte β2 integrin LFA-1 and regulates its activation in leukocytes (ref 105). Our findings show loss of CD47 on leukocytes impairs the innate and adaptive immune systems due to defects in leukocyte recruitment and function in the experimental autoimmune encephalomyelitis (EAE) mouse model of human disease, multiple sclerosis (refs. 88, 105, 115). In a second model, loss of CD47 was predicted to offer protection in a mouse model of atherosclerosis, however, KO animals had greater plaque lesional area due to recruitment of activated NK T cells in aortic arch, despite a significant reduction in other inflammatory leukocytes in lesions (ref 118). We now are investigating how blockade of CD47 binding to SIRPα by antibodies promotes therapeutic efficacy in human hematological cancers and in neuroinflammation (EAE)(ref 115). We have focused on understanding molecular and cellular functions of CD47 antagonists using immune deficient and immune competent mouse models of human T cell lymphoma. We are now testing whether blocking CD47 with an antibody that inhibits only the CD47-SIRP pathway in EAE models, including the acute in B6 mice, relapsing-remitting in SJL mice and progressive EAE in NOD mice, and in immune deficient and immune competent models of T cell lymphoma (ref 119). Summary of Teaching Contributions: I have been actively involved in two pathology courses at Harvard Medical School. I have served as an Instructor and Section Leader in the laboratory and patient cases (9 case presentations) components of the HST-030 course Human Pathology (since 1996 to present, 90 hours/yr). I also served as a Tutorial Leader for the HMS main pathology course IN 754 (from 1998 to 2014, 60 hrs/yr). I also have been on multiple Thesis Advisory Committees and a Thesis Defense Committee for HMS students. Summary of Administrative Contributions: I have a longstanding commitment to the Vascular Research Division (now located within The Center for Excellence in Vascular Biology) in our Department. In addition to guiding the research performed by members of my lab, I assist the Director with strategic planning.