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Haploid embryonic stem cells. R eporter : XueBinghua Date : 2011.12.24. Derivation of haploid embryonic stem cells from mouse embryos. Martin Leeb & Anton Wutz Nature 7 September 2011. R esults:. 1 、 Deriving haploid mouse ES cells. B6CBAF1. 132 activated oocytes 30 blastocysts (22%) - PowerPoint PPT Presentation
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Haploid embryonic stem cells
Reporter: XueBinghuaDate: 2011.12.24
Derivation of haploid embryonic stem cells from mouse embryos
Martin Leeb & Anton WutzNature
7 September 2011
Results:
1 、 Deriving haploid mouse ES cells
B6CBAF1
132 activated oocytes
30 blastocysts (22%)
27 ES cell lines(93%)
LIF+2i[SU5402+PD184352(inhibitor of MAPK and GSK3)]
Requirements for deriving aploid mES cells
1 、 Lif+KOSR+DMEM2 、 Lif+FBS+DMEM+Feeder
Results:
2 、 DNA content
Results:
3 、 Morphology and Karyotype
Results:
4 、 Comparative genomic hybridization
g
CBA control, black; HAP-1, red; HAP-2, green
HTG control, black; HTG-1 , green ; HTG-2 , red
Results:
5 、 Pluripotency markers
Results:
6 、 High correlation : Genome-wide
99 X-linked genes were over expressed and 4 Y-linked genes were
lost in haploid ES cells
Results:
7 、 Differentiation potential :Chimaeras
Results:
8 、 Utility : Genetic screening
1、 6-TG( 2氨基 6巯嘌呤)2、 Spilinkerette PCR