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8/12/2019 Ha See Nullah
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EFFICACY OF DIFFERENT FUNGICIDES ON FRUIT
ROTS OF CHILLI (ALTERNARIA ALTERNATA.) IN
VITRO AND VIVO CONDITION
SUPERVISOR:
Mr. AKHTAR GULCO-SUPERVISOR
MR. FAISAL ADNAN
MEMBER
MR. AHMED KHAN STUDENT:
HASEENULLAH
DEPARTMENT OF PLANT PATHOLOGY
BALOCHISTAN AGRICULTURE COLLEGE, QUETTA
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RATIONALE/PROBLEM STATEMENT
Chilli is grown in four agro-ecological zones of Balochistan
and farmers get high income from chilli growing. However ,
Chilli is susceptible to several diseases particularly fruit rot
of chilli is of great importance in terms of yield and quality
losses. The chemical control of this disease is the easiest
option in farmer’s perspective. So, this study is designed to
evaluate the efficacy of different fungicides against the fruit
rot of Chilli caused by Alternaria alternate.
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OBJECTIVES
To isolate, purify and identify the causal organism of
chilli fruit rot (Aternaria alternata ) and prepare its
culture in the laboratory.
To find out the most effective fungicide against chilli
fruit rot caused by Aternaria alternata under in vitro
and vivo condition.
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INTRODUCTION
Chilli (Capsicum annuum L.) is considered an important tropical
and subtropical crop on the basis of its high consumption,
nutritional and cash value to farmers and consumers both in
developed and developing countries.
In Pakistan chilli (Capsicum annum L.) is an important
vegetable crop planted over an estimated area of 47.3 thousandhectares with an annual production of 69.5 thousand tones, with
an average yield of 1902 kg/ha.
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MATERIAL AND METHODS
Four fungicides i.e. Diathene M45, Topsin, Allete, and Revus will be evaluated against Chilli fruit rot caused by Alternaria
alternata in vitro and vivo condition that will be replicated three
times. In vitro study will be conducted in Plant Pathology
Laboratory Balochistan Agriculture College Quetta using CRD
and in vivo study will be carried out at the experimental field
using RCBD at Directorate of Agriculture Research Plant
Protection, Agriculture Research Institute Sariab Quetta during
2013.
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IN VITRO STUDY
Alteranaria alternata culture will be prepared from infected chilli
fruit that will be used for the assessment of fungicides efficacy.
Different concentrations of the tested fungicides with specific
volumes will be added to the proper amounts of PDA medium
before solidification and then poured into sterilized Petri-dishes
(9cm diam.).
All dishes will be inoculated in the centre from the Alteranaria
alternata cultures.
All dishes will be incubated at 30±1°C for 7 days then the number
of colonies of fungus will be counted and the average will be
calculated.
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IN VIVO STUDY
T1 T3 T4
T2 T5 T2
T3 T1 T3
T4 T2 T5
T5 T4 T1
T1 = No fungicide spray(control)
T2 = Diathene M45 spray
T3 = Topsin spray
T4 = Allete spray
T5 = Revus spray
R1 R2 R3
7 m
6 m
Treatment = 5 (4 fungicides and one control)
Plot size = 6x7 m = 42 m2
Replication = 3
Statistical design = RCBD
Randomized complete block design Water Channel
LAY OUT
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OBSERVATIONS
Number of colonies counted per treatment
In vitro study
In vivo study
Disease severity assessed one week after each spray and theaverage will be calculated
The number of infected fruits/plot
Fruit weight (kg/plot)
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STATISTICAL ANALYSIS
The collected data will be subjected to statistical analysis
using computer software STATISTIX 8.1 (Russel and
Eisensmith, 1983). Treatment averages will be compared at
0.05 level of probability using L.S.D. procedure (Fisher,
1948).
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IN VITRO RESEARCH WORK IS BEING CONDUCTED IN
THE LABORATORY OF PALNT PATHOLOGY
BALOCHISTAN AGRICULTURE COLLEGE QUETTA
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