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GUSTA ME
¿Te gusta la estadística?
An online guide to statistical analysis in microbial ecology
Roman FeldbauerDivision of Computational Systems Biology
GUSTA ME
Motivation
GUSTA ME
End-points
Wizards
Walkthroughs
VIZ libraries
MASAME
Community
Usage ex.
Outlook
● Multivariate statistical analysis in microbial ecology
● Which technique for which data?● Ongoing development of methodology● 'Prevalence rate' of statistical
expertise?
→ Where to find guidance?
GUSTA ME
Motivation
GUSTA ME
End-points
Wizards
Walkthroughs
VIZ libraries
MASAME
Community
Usage ex.
Outlook
● Multivariate statistics for microbial ecologists
● Online resource● Techniques, relevance, debates● Different entry points/approaches
● GUSTA ME webpage:
http://mb3is.megx.net/gustame
GUSTA ME
Motivation
GUSTA ME
End-points
Wizards
Walkthroughs
VIZ libraries
MASAME
Community
Usage ex.
Outlook
● Reference pages● High-level descriptions of techniques● Principles, assumptions, limitations,
statistical and ecological interpretation plus common pitfalls
● Examples: PCA, NMDS, db-RDA, ...
GUSTA ME
Motivation
GUSTA ME
End-points
Wizards
Walkthroughs
VIZ libraries
MASAME
Community
Usage ex.
Outlook
Interactive guides:● Suitable methods for given data set● Decrease and conquer strategy● 'Decision tree' based on (relatively)
easy questions● Suggestion of one or several
appropriate techniques
GUSTA ME
Motivation
GUSTA ME
End-points
Wizards
Walkthroughs
VIZ libraries
MASAME
Community
Usage ex.
Outlook
● Social cognitive theoretic approach● Flowcharts: Analytical methodology
of peer-reviewed studies● Learning by imitation
● http://mb3is.megx.net/gustame/ walkthroughs
GUSTA ME
Motivation
GUSTA ME
End-points
Wizards
Walkthroughs
VIZ libraries
MASAME
Community
Usage ex.
Outlook
● Visualization makes high-dimensional data human readable
● Browsing different types of viz● 'Putting the cart before the horse'
approach
● http://mb3is.megx.net/gustame/home/ visualisations
GUSTA ME
Motivation
GUSTA ME
End-points
Wizards
Walkthroughs
VIZ libraries
MASAME
Community
Usage ex.
Outlook
● Multivariate AnalysiS Applications in Microbial Ecology
● Web front-end for several R tools● Point-and-click style, no R knowledge
required● Example data sets provided● Direct links from end-points
(e.g. PCA, NMDS, db-RDA)
GUSTA ME
Motivation
GUSTA ME
End-points
Wizards
Walkthroughs
VIZ libraries
MASAME
Community
Usage ex.
Outlook
● Community involvement● Online forums
– Comments on content
– Method discussion
– Suggest revisions
– Contribute walkthroughs etc.
● Community still needs to grow...
GUSTA ME
Motivation
GUSTA ME
End-points
Wizards
Walkthroughs
VIZ libraries
MASAME
Community
Usage ex.
Outlook
● Student– Given data set, follow a wizard, analysis using
MASAME, publish study with appropriate multivariate statistics
● Principal investigator– Designing a study, browses walkthroughs, adjusts
sampling design and replication strategy
● Reviewer– Needs to check appropriateness of a certain
technique, jumps directly to corresponding end-point, may check key assumptions with MASAME
GUSTA ME
Motivation
GUSTA ME
End-points
Wizards
Walkthroughs
VIZ libraries
MASAME
Community
Usage ex.
Outlook
● Current state is a starting point, useful resource for multivariate statistics
● Might become great knowledge base● Some issues
– Community involvement
– Minor inconsistencies
– Web design
GUSTA ME
Motivation
GUSTA ME
End-points
Wizards
Walkthroughs
VIZ libraries
MASAME
Community
Usage ex.
Outlook
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