7/24/2019 Guest Lecture Notes

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Dr. Ann West Guest LectureProtein X-Ray Crystallography: The Impact of a Structural Biology Approach

on Signal Transduction Research

wwwrcs!org- Protein "ata Ban#

$Two-Component% Signal Transductiono In !acteria and simple cells& not in higher animal life

o StimulusReceptor ATP'A"P Protein (inase Regulator

)on'o*+ Response, hydrolye the phosphate to gi.e o* Piiandreturns regulator to $o*%

o (inases phosphorylate themsel.es or other molecules, when they

phosphorylate themsel.es auto-phosphorylationo Phosphoryl proteins can ta#e a phosphorylation from a mem!rane

protein to a protein further upstream in the pathwayo Aspartic Acid always relays phosphate to /istidine& .ice .ersa

0P"1 Protein )phosphoryl in yeast+o All helical& si2 alpha-helices

o 3our-heli2 !undle core

o Phosphorylation site located in middle of heli2 alphaC

Phosphorylation of relay protein changes conformation so that /istidine

and Aspartic Acid align to interact

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