Upload
lyle
View
40
Download
0
Tags:
Embed Size (px)
DESCRIPTION
Growth and Cell Division. Growth: Not size, but number of cells Bacteria: Binary fission Yeast: Budding. Cell Division. Bacterial Growth Curve. Lag phase Log phase Stationary phase Death phase. Measuring Bacterial Concentration. Serial dilution/ Standard plate counts - PowerPoint PPT Presentation
Citation preview
1
Growth and Cell Division
Growth:– Not size,
but number of cells
Bacteria:– Binary
fission Yeast:
– Budding
2
Cell Division
3
Bacterial Growth Curve
Lag phase
Log phase
Stationary phase
Death phase
5
Measuring Bacterial Concentration
Serial dilution/ Standard plate counts
CFU = Colony Forming Unit
CFU/ml
8
Measuring Bacterial Growth
Direct microscopic counts
9
Measuring Bacterial Growth Most probable
number
12
Measuring Bacterial Growth
Filtration
Turbidity (Spectrophotometry)
14
Physical Factors Affecting Bacterial Growth
pH Temperature
15
Oxygen
Moisture
Physical Factors Affecting Bacterial Growth
16
Osmotic pressure– Salt
Radiation
Physical Factors Affecting Bacterial Growth
17
Nutritional Factors Affecting Bacterial Growth
Carbon source Nitrogen source Sulfur/ phosphor Trace elements Vitamins Nutritional complexity Enzymes:
– Extracellular– Periplasmic– Cytoplasmic
18
Sporulation
Endospores
Other sporelike bacterial structures
19
Culturing Bacteria — Streak Plates Streak plate method
20
Culturing Bacteria — Pour Plates Pour plate method
21
Culture Media Purposes
Selective media
Differential media
Enrichment media
24
Culture Conditions — O2
Controlling oxygen content
25
Culture conditions
Maintaining cultures
Preserved cultures
Diagnostic tests
Limitations