GRETA MULTIFERMENTER SYSTEM – OPTIMIZE YOUR MICROBIAL PROCESS PARAMETERS

• Sixindividuallycontrolledstainless-steelbioreactorscompactononeframe,eachwith0.2-1Lworkingvolume• Bioreactorsareequippedwithbottommagneticstirrerscombinedwithsparger,andsterilegasfiltersystem• Eachbioreactorisfullyinstrumented:

• ControlledTemperature,pHandDOT• Stirrer,Feedpumps,airandoxygen• Gasflows• OpticalDensityandOutletGasanalyzer

• 1-4MultibioreactormodulesintegratedwithoneCIP/MediapreparationmoduleandonePCcontrolstationprovides6-24state-of-artbioreactors

• InletandoutletgassystemforaerationandDO-control• AutomatedMediapreparation,ReactorSterilization,InductionandHarvest,Cleaning-in-placeandCalibration

sequencesallowonepersonoperation

COMPUTER CONTROL

• User-friendly,opencontrolsystem• Algorithms,dataloggingandprocessadministrationprovidessophisticatedcontroletc.• (FDACFR21Part11)Compliant• CompatiblewithDOEprograms,Excelandotherprograms• Automaticcontrolsequences(e.g.sterilizationsequenceandset-pointprofiles)

GRETA TECHNICAL SPECIFICATION

BIOREACTOR

Volume 6x0.2-1LVesselMaterial StainlesssteelEN1.4404,Ra<0.8

CONTROL SYSTEM

BioPhantom©

GAS SYSTEM

Air IndividualMassFlowControl0-2L/minOxygen/Nitrogen IndividualMassFlowControl0-1L/minPressurereading -1–3barcommonfor6BR(Individualoption)Inlet CommoninletsterilefilterOutlet Refluxcondenser.Commonoutletsterilefilter

TEMPERATURE

TemperatureSensor Pt100MeasuringRange 0-150°CRegulationRange 10-70°CRegulation Proportional-time-regulatorforheatandcool(DB±0.1°C,PB±0.3°C)HeatingElement HotwaterbybottomjacketCoolingElement Tapwater(max12°C)bybottomjacket

STIRRER

SpeedRanges 0-1500rpmRegulation FeedbackregulationMotor 24VDCmotorLocation BottomImpellers 3Rushtonturbines

DISSOLVED OXYGEN (DO)

Electrode Optical325mmpG13.5Range 0-200%±2%Controlby Stirrerspeed,Airflow,O2flow,feedpump

pH

Electrode Combination/glasstype325mmpG13.5Range 2-12±0.05Controlby DualPIDon/offregulationforAcid/Baseadditionwperistalticpump

CONNECTIONS

ExtraInputsignals 8digital,8analogExtraOutputsignals 12digital,4analog

MEDIA

Powersupply 230V,50Hz,10AmpAir Prefiltered1.5to3.0bar,40Nl/min,dryN2 2.0to3.0bar,1Nl/minO2 2.0to3.0bar,1Nl/minCleanSteam 2.5to3.0bar,10kg/hColdtapwater 3.0to4.0bar,100L/h,8to15°CHottapwater 3.0to4.0bar,100L/h,>55°CCleanwater 1.0to3.0bar,100l/h

DIMENSIONS

Height/Width/Diameter 1800/1300/800mmWeight 220kg

OPTIONS INTEGRATED IN BIOPHANTOM SOFTWARE

GasAnalyzerO2andCO2 CP460-O2/CO2gasreadingFeedpump Peristalticpump0.5-200rpmOpticalBiomassMeasurement OnlineOD.0-130g/L

COMPARISON BETWEEN ALL PRODUCT LINESTECHNICAL SPECIFICATION

•–StandardEquipment,▫–NotstandardEquipment,•/▫–OptionalEquipment

LEVEL ANT JENNY HANNA LARS GUSTAV GRETA DOLLY

LevelSensorResistiveprobeø6mm • • • • • • ▫AdditionPump • • • • • • ▫Adjustable-pause-timeregulation • • • • • • ▫

DISSOLVED OXYGEN (DO)

DOSensor:

Electrochemicalpolarographic •/▫ •/▫ •/▫ •/▫ •/▫ •/▫ •/▫Optical •/▫ •/▫ •/▫ •/▫ •/▫ •/▫ ▫

MeasuringRange0-200%-airsaturation • • • • • • ▫DOregulation:

FeedPump •/▫ • • • • • ▫Gascontrol • • • • • • ▫Stirrerspeed • • • • • • ▫

pH

pHSensorElectrochemicalgelelectrode • • • • • • •/▫Additionpumps(acidandbase) • • • • • • •/▫Regulation:twopointproportionaltimeregulationforacid/baseaddition • • • • • • •/▫

GAS SYSTEM

Rotameterfixedregulation • • • ▫ ▫ ▫ •/▫MassflowmeterforAir • •/▫ •/▫ • • • ▫MassflowmeterforO2/N2/CO2

▫ •/▫ •/▫ •/▫ •/▫ • ▫Outletgascondenser • • • • • • •

Inletfilter • • • • • • •/▫Outletfilter • • • • • • •/▫VolumetricGasMeasurement ▫ ▫ ▫ ▫ ▫ ▫ •

CONTROL CONNECTIONS

ExtraInputsignals - 6analog 6analog 6analog 6analog 8analog8digital 6analog

ExtraOutputsignals - 4analog6digital

4analog6digital

4analog6digital

4analog6digital

4analog12digital

4analog6digital

MEDIA REQUIRMENTS

ElectricPower230VAC±10V 230V 230V 230V 230V 3phase 3phase 230V

TapWater • • • • • • •

HotWater(55°C) ▫ ▫ ▫ ▫ ▫ • ▫PressurizedAir •/▫ • • • • • •/▫Steam ▫ ▫ ▫ • • • ▫

DIMENSIONS

Height(mm) 650 330 360 1650 2250 1800 1500

Width(mm) 300 360 450 1045 1900 1300 600

Length(mm) 450 430 520 600 1050 800 600

Weight(kg) 18 16 18 - - 220 70

BIOREACTOR ANT JENNY HANNA LARS GUSTAV GRETA DOLLY

Volume(L) 0.2-5 0.2-2 1-10 3-30 50-1000 6x0.2-1.0 2x2-6

VesselMaterial:

ASTM316L ▫ ▫ ▫ • • • ▫Glass • • • ▫ ▫ ▫ •

APPLICATION

Microbial • • • • • • ▫Cell ▫ ▫ • • ▫ ▫ ▫Funghi/Yeast • • • • • • ▫Biofuel ▫ ▫ ▫ ▫ ▫ ▫ •

Parallel ▫ ▫ ▫ ▫ ▫ • •

Fed-batch • • • • • • •

Continuouscultivation •/▫ •/▫ •/▫ •/▫ •/▫ ▫ ▫Perfusionwithe.g.ATF • • • • • ▫ ▫

CONTROL SYSTEM

BioPhantom ▫ • • • • • •

WebAnt • ▫ ▫ ▫ ▫ ▫ ▫

TEMPERATURE

TemperatureSensor:

Pt100inprobe • • ▫ ▫ ▫ •

Pt100inpocket • ▫ ▫ • • • ▫RegulationRange(°C) 10-70 10-70 10-70 •

Proportional-time-regulatorforheatandcool • • • • • • •

Heating:

Heatingelement ▫ ▫ ▫ • • ▫ ▫100-150Wheatingband • • • ▫ •

Jacketbysteamheatexchanger ▫ ▫ ▫ • • ▫ ▫Hotwaterthroughjacket ▫ ▫ ▫ ▫ ▫ • ▫

Cooling:

Tapwaterincoolingfinger • ▫ • ▫ ▫ ▫ •

Tapwaterinjacket ▫ • ▫ • • • ▫

STIRRER

SpeedRanges(rpm): 150-1500 150-1500 150-1500(20-300) 150-1500 50-800 50-1500 20-250

Motor(Watt/Nm): 40/0.3 40/0.3 40/0.3 750/2 5000/3 30/0.2 40/1.5

Magnetcoupledbottomstirrer ▫ ▫ • • • • ▫Lipsealedtopstirrer • • ▫ ▫ ▫ ▫ •

Location:

Topmounted(lipseal) • ▫ • ▫ •/▫ ▫ •

Bottommounted(magneticcoupling) ▫ • ▫ • • • ▫

PRESSURE

Reading ▫ ▫ ▫ • • •(common)•/▫ (ind) ▫

Regulationvalve ▫ ▫ ▫ •/▫ •/▫ •/▫ (ind) ▫

CLEANING IN PLACE (CIP) ▫ ▫ ▫ •/▫ •/▫ • ▫

STERILIZATION (SIP) ▫ ▫ ▫ • • • ▫