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Graphs and polytopes: learning Bayesian networks with LP
relaxations
Tommi JaakkolaMIT CSAIL
based on joint work withDavid Sontag, MIT
Amir Globerson, HUJIMarina Meila, U Washington
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
• Bayesian networks are widely used as modeling tools across applied areas, including computational biology
• Learned structures (especially causal) can lead to useful insights about the domain
Learning Bayesian networks
sample in this data set consists of quantita-tive amounts of each of the 11 phosphorylatedmolecules, simultaneously measured from sin-gle cells [data sets are downloadable (8)]. Forpurposes of illustration, examples of actualfluorescence-activated cell sorter (FACS) dataplotted in prospective corelationship form areshown in fig. S1. In most cases, this reflects theactivation state of the kinases monitored, or inthe cases of PIP3 and PIP2, the levels of thesesecondary messenger molecules in primary cells,under the condition measured. Nine stimula-tory or inhibitory interventional conditions wereused (Table 1) (8). The complete data sets wereanalyzed with the Bayesian network structureinference algorithm (6, 9, 17).
A high-accuracy human primary T cellsignaling causality map. The resulting denovo causal network model was inferred(Fig. 3A) with 17 high-confidence causal arcsbetween various components. To evaluate thevalidity of this model, we compared the mod-el arcs (and absent potential arcs) with thosedescribed in the literature. Arcs were catego-rized as the following: (i) expected, for con-nections well-established in the literature thathave been demonstrated under numerous con-ditions in multiple model systems; (ii) reported,for connections that are not well known, butfor which we were able to find at least oneliterature citation; and (iii) missing, which indi-cates an expected connection that our Bayesiannetwork analysis failed to find. Of the 17 arcsin our model, 15 were expected, all 17 wereeither expected or reported, and 3 were missed(Fig. 3A and table S1) (8, 18–22). Table 3enumerates the probable paths of influencecorresponding to model arcs determined bysurveying published reports.
Several of the known connections fromour model are direct enzyme-substrate rela-tionships (Fig. 3B) (PKA to Raf, Raf to Mek,Mek to Erk, and Plc-g to PIP2), and one has arelationship of recruitment leading to phos-phorylation (Plc-g to PIP3). In almost all cases,
the direction of causal influence was correctlyinferred (an exception was Plc-g to PIP3, inwhich case the arc was inferred in the reversedirection). All the influences are containedwithin one global model; thus, the causal di-rection of arcs is often compelled so that theseare consistent with other components in themodel. These global constraints allowed de-tection of certain causal influences from mole-cules that were not perturbed in our assay.For instance, although Raf was not perturbedin any of the measured conditions, the meth-od correctly inferred a directed arc from Rafto Mek, which was expected for the well-characterized Raf-Mek-Erk signal transduc-tion pathway. In some cases, the influence ofone molecule on another was mediated by in-termediate molecules that were not measuredin the data set. In the results, these indirectconnections were detected as well (Fig. 3B,panel b). For example, the influence of PKAand PKC on the MAPKs p38 and Jnk likelyproceeded via their respective (unmeasured)MAPK kinase kinases. Thus, unlike someother approaches used to elucidate signalingnetworks [for example, protein-protein inter-action maps (23, 24)] that provide static bio-chemical association maps with no causallinks, our Bayesian network method can de-tect both direct and indirect causal connectionsand therefore provide a more contextual pic-ture of the signaling network.
Another feature demonstrated in our mod-el is the ability to dismiss connections thatare already explained by other network arcs(Fig. 3B, panel c). This is seen in the Raf-Mek-Erk cascade. Erk, also known as p44/42,is downstream of Raf and therefore dependenton Raf, yet no arc appears from Raf to Erk,because the connection from Raf to Mek andthe connection from Mek to Erk explain thedependence of Erk on Raf. Thus, an indirectarc should appear only when one or moreintermediate molecules is not present in thedata set, otherwise the connection will proceed
via this molecule. The intervening moleculemay also be a shared parent. For example,the phosphorylation statuses of p38 and Jnkare correlated (fig. S2), yet they are not di-rectly connected, because their shared parents(PKC and PKA) mediate the dependence be-tween them. Although we cannot know wheth-er an arc in our model represents a direct orindirect influence, it is unlikely that our modelcontains an indirect arc that is mediated byany molecule observed in our measurements.Correlation exists between most moleculepairs in this data set [per Bonferroni correctedP value (fig. S2)], which can occur with close-ly connected pathways. Therefore, the relativelack of arcs in our model (Fig. 3A) contrib-uted greatly to the accuracy and interpret-ability of the inferred model.
A more complex example is the influenceof PKC on Mek, which is known to be me-diated by Raf (Fig. 3B, panel d). PKC isknown to affect Mek through two paths ofinfluence, each mediated by a different ac-tive phosphorylated form of the protein Raf.Although PKC phosphorylates Raf directlyat S499 and S497, this event is not detectedby our measurements, because we use onlyan antibody specific to Raf phosphorylationat S259 (Table 2) (16). Therefore, our algo-rithm detects an indirect arc from PKC toMek that is mediated by the presumed un-measured intermediate Raf phosphorylatedat S497 and S499 (18). The PKC-to-Raf arcrepresents an indirect influence that proceedsvia an unmeasured molecule, presumed to beRas (19, 20). We discussed above the abilityof our approach to dismiss redundant arcs. Inthis case, there are two paths leading fromPKC to Mek, because each path correspondsto a separate means of influence from PKCto Mek: one via Raf phosphorylated at S259and the other through Raf phosphorylated atS497 and S499. Thus, neither path is redun-dant. This result demonstrates the distinctionthat this analysis is sensitive to specific phos-
Fig. 3. Bayesian network inferenceresults. (A) Network inferred fromflow cytometry data represents ex-pected outcomes. This network rep-resents a model average from 500high-scoring results. High-confidencearcs, appearing in at least 85% ofthe networks, are shown. For clarity,the names of the molecules are usedto represent the measured phospho-rylation sites (Table 2). (B) Inferrednetwork demonstrates several fea-tures of Bayesian networks. (a) Arcsin the network may correspond todirect events or (b) indirect influ-ences. (c) When intermediate mol-ecules are measured in the dataset, indirect influences rarely appear as an additional arc. No additionalarc is added between Raf and Erk because the dependence between Rafand Erk is dismissed by the connection between Raf and Mek, and be-tween Mek and Erk (for instance, see Fig. 1C). (d) Connections in themodel contain phosphorylation site–specificity information. Because Raf
phosphorylation on S497 and S499 was not measured in our data set,the connection between PKC and the measured Raf phosphorylation site(S259) is indirect, likely proceeding via Ras. The connection between PKCand the undetected Raf phosphorylation on S497 and S499 is seen as anarc between PKC and Mek.
R E S E A R C H A R T I C L E S
22 APRIL 2005 VOL 308 SCIENCE www.sciencemag.org526
on
Ap
ril 1
3,
20
09
w
ww
.scie
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g.o
rgD
ow
nlo
ad
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fro
m
(Sachs et al. 2005)
themselves transcriptionally regulated. Theseregulation mechanisms often involve feed-forward loops and feedback mechanisms(18, 31) that change the mRNA expression
level of regulators when their protein activ-ity changes. As a consequence, we candetect coordinated changes in the expres-sion levels of regulators and their targets.
This hypothesis is supported by an analysisof the discovered regulatory relations againsta database of protein-DNA and protein-protein interactions (26).
Fig. 3. Different regulatory network architectures. (A) An uncon-strained acyclic network where each gene can have a different regu-lator set. This is a fragment of a network learned in the experimentsof Pe’er et al. (24 ). (B) A summary of direct neighbor relations amongthe genes shown in (A) based on bootstrap estimates. Degrees ofconfidence are denoted by edge thickness. We automatically identifya subnetwork of genes, with high-confidence relations among them,that are involved in the yeast-mating pathways. The colors highlightgenes with known function in mating, including signal transduction(yellow), transcription factors (blue), and downstream effectors(green). (C) A fragment of a two-level network described by Pe’er etal. (25). The top level contains a small number of regulators; the
bottom level contains all other genes (targets). Each gene has differ-ent regulators from among the regulator genes. (D) Visualization ofsignificant Gene Ontology (42) annotations of the targets of differentregulators. Each significant annotation for the targets of a regulator(or pairs of regulators) is shown with the hypergeometric p-value. (E)A fragment of the module network described by Segal et al. (26). Eachmodule contains several genes that share the same set of regulators andshare the same conditional regulation program given these regulators. (F)Visualization of the expression levels of the 55 genes in Module 1 (b) andtheir regulators (a). Significant Gene Ontology annotations (c) and cis-regulatory motifs in promoter regions of genes in the module (d) are shown.[See figure 3 of (26); reproduced with permission]
M A T H E M A T I C S I N B I O L O G Y
6 FEBRUARY 2004 VOL 303 SCIENCE www.sciencemag.org804
SPECIALSECTION
on
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27
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00
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ww
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(Friedman 2004)
MATING_TYPE
FUS3
STE50FUS1
TUP1
STE7
MFA1
STE2 AGA2
STE20
GPA1
BAR1FAR1
SAG1STE18
STE6
STE3
SST2
MFA2
AGA1 SIN3STE4
SNF2
MFALPHA1
SWI1TEC1 KSS1MCM1
STE5
MFALPHA2
KAR3
STE12
STE11
MATING_TYPE
FUS3
STE50
TUP1
STE7
MFA1
STE2
AGA2
GPA1
STE12
BAR1
SAG1
STE18
STE6
STE3
SST2
MFA2
AGA1
SIN3
SNF2 MFALPHA1
SWI1
TEC1
FUS1 KSS1 STE20 MCM1
STE5
MFALPHA2
KAR3
STE4
FAR1
STE11
Figure 2. Bayesian network models learned by model averaging over the 500 highest scoringmodels visited during the unconstrained and constrained simulated annealing search runs,respectively. Edges are included in the figure if and only if their posterior probabilityexceeds 0.5. Node and edge color descriptions are included in the text.
(Hartemink et al. 2002)
...
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
Structure learning: basics
themselves transcriptionally regulated. Theseregulation mechanisms often involve feed-forward loops and feedback mechanisms(18, 31) that change the mRNA expression
level of regulators when their protein activ-ity changes. As a consequence, we candetect coordinated changes in the expres-sion levels of regulators and their targets.
This hypothesis is supported by an analysisof the discovered regulatory relations againsta database of protein-DNA and protein-protein interactions (26).
Fig. 3. Different regulatory network architectures. (A) An uncon-strained acyclic network where each gene can have a different regu-lator set. This is a fragment of a network learned in the experimentsof Pe’er et al. (24 ). (B) A summary of direct neighbor relations amongthe genes shown in (A) based on bootstrap estimates. Degrees ofconfidence are denoted by edge thickness. We automatically identifya subnetwork of genes, with high-confidence relations among them,that are involved in the yeast-mating pathways. The colors highlightgenes with known function in mating, including signal transduction(yellow), transcription factors (blue), and downstream effectors(green). (C) A fragment of a two-level network described by Pe’er etal. (25). The top level contains a small number of regulators; the
bottom level contains all other genes (targets). Each gene has differ-ent regulators from among the regulator genes. (D) Visualization ofsignificant Gene Ontology (42) annotations of the targets of differentregulators. Each significant annotation for the targets of a regulator(or pairs of regulators) is shown with the hypergeometric p-value. (E)A fragment of the module network described by Segal et al. (26). Eachmodule contains several genes that share the same set of regulators andshare the same conditional regulation program given these regulators. (F)Visualization of the expression levels of the 55 genes in Module 1 (b) andtheir regulators (a). Significant Gene Ontology annotations (c) and cis-regulatory motifs in promoter regions of genes in the module (d) are shown.[See figure 3 of (26); reproduced with permission]
M A T H E M A T I C S I N B I O L O G Y
6 FEBRUARY 2004 VOL 303 SCIENCE www.sciencemag.org804
SPECIALSECTION
on
Fe
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27
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complete data
highest scoringacyclic graph
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
Structure learning: basics
themselves transcriptionally regulated. Theseregulation mechanisms often involve feed-forward loops and feedback mechanisms(18, 31) that change the mRNA expression
level of regulators when their protein activ-ity changes. As a consequence, we candetect coordinated changes in the expres-sion levels of regulators and their targets.
This hypothesis is supported by an analysisof the discovered regulatory relations againsta database of protein-DNA and protein-protein interactions (26).
Fig. 3. Different regulatory network architectures. (A) An uncon-strained acyclic network where each gene can have a different regu-lator set. This is a fragment of a network learned in the experimentsof Pe’er et al. (24 ). (B) A summary of direct neighbor relations amongthe genes shown in (A) based on bootstrap estimates. Degrees ofconfidence are denoted by edge thickness. We automatically identifya subnetwork of genes, with high-confidence relations among them,that are involved in the yeast-mating pathways. The colors highlightgenes with known function in mating, including signal transduction(yellow), transcription factors (blue), and downstream effectors(green). (C) A fragment of a two-level network described by Pe’er etal. (25). The top level contains a small number of regulators; the
bottom level contains all other genes (targets). Each gene has differ-ent regulators from among the regulator genes. (D) Visualization ofsignificant Gene Ontology (42) annotations of the targets of differentregulators. Each significant annotation for the targets of a regulator(or pairs of regulators) is shown with the hypergeometric p-value. (E)A fragment of the module network described by Segal et al. (26). Eachmodule contains several genes that share the same set of regulators andshare the same conditional regulation program given these regulators. (F)Visualization of the expression levels of the 55 genes in Module 1 (b) andtheir regulators (a). Significant Gene Ontology annotations (c) and cis-regulatory motifs in promoter regions of genes in the module (d) are shown.[See figure 3 of (26); reproduced with permission]
M A T H E M A T I C S I N B I O L O G Y
6 FEBRUARY 2004 VOL 303 SCIENCE www.sciencemag.org804
SPECIALSECTION
on
Fe
bru
ary
27
, 2
00
8
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complete data
decomposable scoring function
highest scoringacyclic graph
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
Structure learning as inference
1
2 3
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
Structure learning as inference
1
2 3
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
Structure learning as inference
1
2 3
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
Structure learning as inference
1
2 3
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
1
2 3
Structure learning as inference
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
Score based approaches (briefly)• Local search methods
- stochastic search (e.g., Heckerman et al., 1995)- over equivalence classes (e.g., Chickering 2002)- order based search (e.g., Teyssier et al., 2005)
• Exact search methods- dynamic programming (e.g., Koivisto et al., 2004, Singh et al.,
2005, Silander et al., 2006)- partial order covers (Parviainen et al., 2009)- branch and bound (e.g., de Campos et al., 2009 )
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
• Dynamic programming methods work well for small structure learning problems ...
20 25 30 35 4010
0
101
102
103
104
number of variables
seconds
Exact methods
2 sec
24 sec
32 min
dynamicprogramming
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
• Dynamic programming methods work well for small structure learning problems ...
20 25 30 35 4010
0
101
102
103
104
number of variables
seconds
Exact methods
2 sec
24 sec
32 min
dynamicprogramming
it should be possible tosolve some larger
problems much faster...
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
• We reduce the search over graph structures to a linear program over a polytope representing acyclic graphs- each vertex corresponds to an acyclic graph- interior points correspond to distributions over graphs
• Any solution obtained at a vertex is guaranteed to be optimal (“certificate of optimality”)
score
Overview of our approach
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
Graphs and vectors
1
2 3
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
Graphs and vectors
100000010100
parent set selectionfor variable 1
parent set selectionfor variable 2
parent set selectionfor variable 3
1
2 3
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
Graphs and vectors
100000010100
parent set selectionfor variable 1
parent set selectionfor variable 2
parent set selectionfor variable 3
1
2 3
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
Graphs and polytopes1
2 3
100000010100
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
Graphs and polytopes1
2 3
1
2 3
100000010100
010000101000
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
Graphs and polytopes1
2 3
1
2 3
100000010100
010000101000
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
Graphs and polytopes1
2 3
1
2 3
100000010100
010000101000
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
Graphs and polytopes1
2 3
1
2 3
100000010100
010000101000
1
2 3
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
• Maximize
subject to
• Integral solution is optimal (“certificate of optimality”)
LP for structure learning
“expected” score
vertices are binaryvectors corresponding
to acyclic graphs
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
• Maximize
subject to
• Integral solution is optimal (“certificate of optimality”)
• But the polytope has exponentially many facets...
LP for structure learning
“expected” score
vertices are binaryvectors corresponding
to acyclic graphs
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
A cutting plane approach• We only need to fully characterize the polytope (linear
constraints) near the actual solution
parent set selectionprobabilities
scoresimple
constraints
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
A cutting plane approach• We only need to fully characterize the polytope (linear
constraints) near the actual solution- solve first with the current constraints
parent set selectionprobabilities
current solutionscoresimple
constraints
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
A cutting plane approach• We only need to fully characterize the polytope (linear
constraints) near the actual solution- solve first with the current constraints- find a violated constraint (separation problem)
parent set selectionprobabilities
current solutionscoresimple
constraints
violated simple constraint
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
A cutting plane approach• We only need to fully characterize the polytope (linear
constraints) near the actual solution- solve first with the current constraints- find a violated constraint (separation problem)- resolve
parent set selectionprobabilities
current solution
new solution
score
violated simple constraint
simpleconstraints
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
A cutting plane approach• We only need to fully characterize the polytope (linear
constraints) near the actual solution- solve first with the current constraints- find a violated constraint (separation problem)- resolve
parent set selectionprobabilities
current solutionscore
violated simple constraint
simpleconstraints
What are the linear constraints?How to find a violated constraint?How to solve the resulting (simpler) LP?
new solution
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
The separation problem1
2 3
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
The separation problem1
2 3
010001000010
parent set selectionfor variable 1
parent set selectionfor variable 2
parent set selectionfor variable 3
100010001000
In an acyclic graph, any subset (cluster) C must have
at least one variable withall its parents outside C
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
LP relaxation for structure learning• Maximize
subject to parent set selections
cluster constraints
“expected” score
These constraints are facet defining but not sufficient to fully specify the polytope
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
Dual LP for structure learning • Minimize
subject to
local scores adjusted based on clusters
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
Dual LP for structure learning • Minimize
subject to
•Why the dual?- simpler constraints, closed
form coordinate updates
- clusters can be ranked by their effect on value
- any dual feasible point upper bounds the LP value (and the optimal score)
local scores adjusted based on clusters
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
Simple example problem• RNAi gene silencing experiments in C. elegans
• 25 phenotypic indicators/markers that characterize each experimental outcome
• We seek to build a Bayesian network model over the phenotypic markers in order to capture their coordinate variation across experiments
• Technical constraint: each variable can have at most four parents
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
• The LP is tight if we can find a structure (integral solution) that attains the dual value
0 50 100 150 200−6300
−6200
−6100
−6000
−5900
−5800
−5700
−5600
−5500
Dual solution
dual parameter updates
score
cluster constraintsadded
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
• We can use adjusted local scores from the dual solution to find good candidate structures (decoding)
Minimum regret decoding
local scores adjusted based on clusters
find an ordering of variables
select parents based on the ordering
candidate structure
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
0 0.5 1 1.5 2 2.5 3 3.5 4 4.5!6300
!6200
!6100
!6000
!5900
!5800
!5700
!5600
!5500
seconds
score
Dual solution with decoding
• A structure is proved optimal if it attains the dual value
• The simple LP relaxation is (almost) tight for this problem
best integral reconstruction
dual value
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
• We can further tighten the approximation by iteratively partitioning the space of possible solutions and using the LP relaxation separately for each partition
• The dual LP (upper bound) is particularly effective for determining how the tree of partitions should be expanded
Branch and Bound
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
Partitions
parent set selectionprobabilities
current solution
score
cluster constraints
• We can tighten the approximation further by partitioning the polytope into segments and solving each segment separately
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
• We can tighten the approximation further by partitioning the polytope into segments and solving each segment separately
Partitions
parent set selectionprobabilities
current solution
score
(1)
(2)cluster constraints
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
• We can tighten the approximation further by partitioning the polytope into segments and solving each segment separately
parent set selectionprobabilities
Partitions
score
(1)
(2)
new solution
cluster constraints
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
parent set selectionprobabilities
Partitions
score
(1)
(2)
new solution
cluster constraints
• We can tighten the approximation further by partitioning the polytope into segments and solving each segment separately
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
Solution...• The highest scoring structure is found after a small
number of branch and bound partitions
0 50 100 150 200 250!6095
!6090
!6085
!6080
!6075
!6070
!6065
!6060
branch and bound expansions
score
LP value
best integral reconstruction
8 seconds
certificate ofoptimality
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
• Dynamic programming methods work well for small structure learning problems ...
20 25 30 35 4010
0
101
102
103
104
number of variables
seconds
Recall: exact methods
2 sec
24 sec
32 min
dynamicprogramming
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
LP relaxation with BB• LP relaxation combined with branch and bound
performs similarly to dynamic programming methods
20 25 30 35 4010
0
101
102
103
104
number of variables
seconds
2 sec
24 sec
32 min
8 sec
21 mindynamic
programminglinear
programming withbranch and bound
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
The alarm network• 37 variables, 1000 data points
case # x1 x2 x3 x37
1
2
3
4
10,000
3
2
1
3
2
3
2
3
2
2
2
2
3
3
2
4
3
3
1
3
!
!
" #
17
25
6 5 4
19
27
20
10 21
37
31
11 32
33
22
15
14
23
13
16
29
8 9
2812
34 35 36
24
30
72618
321 (a)
(b)
17
25 18 26
3
6 5 4
19
27
20
10 21
35 3736
31
11 32 34
1224
33
22
15
14
23
13
16
29
30
7 8 9
28
21
(c)
17
25
6 5 4
19
27
20
10 21
37
31
11 32
33
22
15
14
23
13
16
29
8 9
2812
34 35 36
24
30
72618
321 (d)
deleted
(Heckerman et al. 1995)
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
The alarm network
20 25 30 35 4010
0
101
102
103
104
number of variables
seconds
2 sec
24 sec
32 min
8 sec
21 min
40 hours
dynamicprogramming
linearprogramming withbranch and bound
• Dynamic programming methods are no longer practical without additional constraints ...
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
22 24 26 28 30 32 34 36 3810
0
101
102
103
104
number of variables
seconds
The alarm network
2 sec
24 sec
32 min
8 sec
21 min
40 hours
7 min
dynamicprogramming
linearprogramming withbranch and bound
• Dynamic programming methods are no longer practical without additional constraints ...
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
22 24 26 28 30 32 34 36 3810
0
101
102
103
104
number of variables
seconds
The alarm network
2 sec
24 sec
32 min
8 sec
21 min
40 hours
7 min
dynamicprogramming
linearprogramming withbranch and bound
300x
• Dynamic programming methods are no longer practical without additional constraints ...
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
0 500 1000 1500 2000 2500 3000 3500 4000 4500!10000
!9950
!9900
!9850
!9800
!9750
branch and bound expansions
score
Anytime solution
LP value
best integral reconstruction
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
0 500 1000 1500 2000 2500 3000 3500 4000 4500!10000
!9950
!9900
!9850
!9800
!9750
branch and bound expansions
score
Anytime solution
LP value
best integral reconstruction
optimal solutionfound
certificate ofoptimality
2 min 7 min
Wednesday, March 10, 2010
Small scale validation cont’d• We can also directly examine changes due to OR1 knock-out
Preliminary validation• The !-phage (bacteria infecting virus)!"#$%&'#($)*#+,-./
0#,12(#'
3-142(#
5&1-6#
7&8.6,
OR3 OR1OR2cI cro
cI RNAp2
Arkin et al. 1998
• We can find the equilibrium of the game (binding frequencies)as a function of overall protein concentrations.
50
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
3
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(a) OR3
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
2
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(b) OR2
10!2
100
102
0
0.2
0.4
0.6
0.8
1
Binding in OR
1
frepressor
/fRNA
Bin
din
g F
req
ue
ncy (
tim
e!
ave
rag
e)
RepressorRNA!polymerase
(c) OR1
Figure 3: Predicted protein binding to sites OR3, OR2, and mutated OR1 for increasing amounts of cI2.
became su!ciently high do we find cI2 at the mutatedOR1 as well. Note, however, that cI2 inhibits transcrip-tion at OR3 prior to occupying OR1. Thus the bindingat the mutated OR1 could not be observed without in-terventions.
7 Discussion
We believe the game theoretic approach provides a com-pelling causal abstraction of biological systems with re-source constraints. The model is complete with prov-ably convergent algorithms for finding equilibria on agenome-wide scale.
The results from the small scale application are en-couraging. Our model successfully reproduces knownbehavior of the !!switch on the basis of molecularlevel competition and resource constraints, without theneed to assume protein-protein interactions between cI2dimers and cI2 and RNA-polymerase. Even in the con-text of this well-known sub-system, however, few quan-titative experimental results are available about bind-ing. Proper validation and use of our model thereforerelies on estimating the game parameters from availableprotein-DNA binding data (in progress). Once the gameparameters are known, the model provides valid pre-dictions for a number of possible perturbations to thesystem, including changing nuclear concentrations andknock-outs.
Acknowledgments
This work was supported in part by NIH grant GM68762and by NSF ITR grant 0428715. Luis Perez-Breva is a“Fundacion Rafael del Pino” Fellow.
References
[1] Adam Arkin, John Ross, and Harley H. McAdams.Stochastic kinetic analysis of developmental path-way bifurcation in phage !-infected excherichia colicells. Genetics, 149:1633–1648, August 1998.
[2] Kenneth J. Arrow and Gerard Debreu. Existence ofan equilibrium for a competitive economy. Econo-metrica, 22(3):265–290, July 1954.
[3] Z. Bar-Joseph, G. Gerber, T. Lee, N. Rinaldi,J. Yoo, B. Gordon F. Robert, E. Fraenkel,T. Jaakkola, R. Young, and D. Gi"ord. Compu-tational discovery of gene modules and regulatorynetworks. Nature Biotechnology, 21(11):1337–1342,2003.
[4] Otto G. Berg, Robert B. Winter, and Peter H. vonHippel. Di"usion- driven mechanisms of proteintranslocation on nucleic acids. 1. models and theory.Biochemistry, 20(24):6929–48, November 1981.
[5] Drew Fudenberg and Jean Tirole. Game Theory.The MIT Press, 1991.
10
• Predictions are again qualitatively correct
52
Summary• Finding the highest scoring Bayesian network structure
from data is a hard combinatorial problem... but the “hard instances” may not be typical
• Our “anytime” approach to structure learning is based on linear programming relaxations that are iteratively refined in a cutting plane fashion
• The approach relies fundamentally on understanding the facets of the polytope corresponding to acyclic graphs
Wednesday, March 10, 2010