1
Drought affects morphological, chemicals and physiological mechanism in wheat. The yield and harvest index are heavily reduced when drought stress is imposed during and after anthesis (Bauder, 2001; Gupta et al., 2001). It has been reported that reduction of 40% water can cause 20.6 % yield reduction in wheat. Maximum partitioning of assimilates to spike is crucial to increase grain number, above ground dry matter and harvest index (HI) to raise yield under drought conditions. Genetic improvement is the most effective and sustainable method to achieve this goal. Genetic Variability and Association Analysis in US Soft Wheat Panel for Fruiting Efficiency Under Post Anthesis Drought and Supplemental Irrigated Conditions Jahangir Khan 1 , Sumit Pradhan Shrestha 1 , Muhsin Ibrahim Avci 1 , Dipendra Shahi 1 , Jia Guo 1 , Maksud Morshed Hossain 1 , Mohammad Atikur Rahman 1 , Ann Blount 2 and Md Ali Babar 1 1 Department of Agronomy, University of Florida, 2 North Florida Research and Education Centre, Quincy, FL Bibliography Experimental design: Modified Augmented design with 3 repeated checks Drought imposed after Anthesis Sowing date: November, 23-30 2016 Harvesting date: May 10-18, 2017 Fruiting Efficiency Fruiting efficiency is considered to be a potential venue for increasing grain number under stress and non stress conditions Ratio of grain number to spike dry chaff weight Introduction Results Materials & Methods Objectives Study genetic source of variations for fruiting efficiency, grain number, yield and HI in US soft wheat association panel under post-anthesis drought stress. Identify traits and mechanisms determining genetic variation in fruiting efficiency (FE). Significant genetic variability and good correlation observed for spike fertility, harvest index and grain yield traits under drought conditions. Strong correlations were observed between FE with harvest index and grain yield. Identification of loci involved with higher FE and related traits in soft wheat is in progress. Understanding of physiological basis of increasing fruiting efficiency and the contributing of different spike components is in progress. Bauder, J. 2001. Irrigating with limited water supplies. Montana State Univ. Comm. Ser. Montana Hall. Bozeman, MT 59717, USA. Daryanto, S., Wang, L., & Jacinthe, P.-A. (2016). Global Synthesis of Drought Effects on Maize and Wheat Production. PLoS ONE, 11(5). Gupta, N.K., S. Gupta and A. Kumar. 2001. Effect of water stress on physiological attributes and their relationship with growth and yield of wheat cultivars at different stages. J. Agron. Crop Sci. 186: 55-62. Conclusion Figure 1. Citra and Quincy Figure 2. Rainfall Citra and Quincy 1.33 3.8 3.2 1.24 3.24 5.95 MARCH APRIL MAY Rainfall (inch) Citra Quincy Acknowledgment Source Grain Yield (g) Harvest Index % Fruiting Efficiency Thousand Grain Wt. (g) Checks <0.0001 <0.0001 <0.0001 <0.0001 Location <0.0001 <0.0001 <0.0001 <0.0001 Block x Location 0.2177 0.2571 0.6814 0.2845 New x Entry x Location 0.9095 0.9603 0.2137 0.6763 New x Entry 0.0062 0.0004 0.0052 <0.0001 Table 1. Combined Analysis of Variance Significant (P<0.05) genetic variations differences were observed for grain yield, fruiting efficiency, harvest index, and thousand grain weight. Figure 4. Regression Analysis of FE and related traits Plant Material: 300 soft wheat genotypes Location: Citra and Quincy , Florida y = 0.1784x + 4.4899 R² = 0.2511 0 20 40 60 80 100 120 140 160 180 0 100 200 300 400 500 600 Fruit efficiency Grain Yield y = 1.6955x - 2.046 R² = 0.2258 0 20 40 60 80 100 120 140 160 180 0 10 20 30 40 50 Fruit efficiency Harvest Index % y = 0.3772x - 50.54 R² = 0.4849 0 100 200 300 400 500 600 0 200 400 600 800 1000 1200 1400 Grain Yield Total Dry Matter y = 8.2651x + 1.1357 R² = 0.6803 0 100 200 300 400 500 600 0 10 20 30 40 50 Grain Yield Harvest Index Preliminary Results form two locations showed R 2 values of 0.2511 and 0.2258 for fruiting efficiency with grain yield and harvest index Figure 3. Histogram for Lsmeans of FE PCA for both locations showed positive correlation between FE and other related related traits and PC1 accounted for 56.9 % of variation. 0 20 40 60 80 100 120 140 160 180 Fruit efficiency Genotype

Genetic Variability and Association Analysis in US Soft ... · Jahangir Khan1, Sumit Pradhan Shrestha1, Muhsin Ibrahim Avci1, Dipendra Shahi1, Jia Guo1, Maksud Morshed Hossain1, Mohammad

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Page 1: Genetic Variability and Association Analysis in US Soft ... · Jahangir Khan1, Sumit Pradhan Shrestha1, Muhsin Ibrahim Avci1, Dipendra Shahi1, Jia Guo1, Maksud Morshed Hossain1, Mohammad

➢Drought affects morphological, chemicalsand physiological mechanism in wheat.

➢The yield and harvest index are heavilyreduced when drought stress is imposedduring and after anthesis (Bauder, 2001;Gupta et al., 2001).

➢ It has been reported that reduction of 40%water can cause 20.6 % yield reduction inwheat.

➢Maximum partitioning of assimilates tospike is crucial to increase grain number,above ground dry matter and harvest index(HI) to raise yield under drought conditions.

➢Genetic improvement is the most effective

and sustainable method to achieve this goal.

Genetic Variability and Association Analysis in US Soft Wheat Panel for Fruiting

Efficiency Under Post Anthesis Drought and Supplemental Irrigated Conditions

Jahangir Khan1, Sumit Pradhan Shrestha1, Muhsin Ibrahim Avci1, Dipendra Shahi1, Jia Guo1, Maksud Morshed Hossain1,

Mohammad Atikur Rahman1, Ann Blount2 and Md Ali Babar1

1Department of Agronomy, University of Florida, 2North Florida Research and Education Centre, Quincy, FL

Bibliography

• Experimental design: Modified Augmented

design with 3 repeated checks

• Drought imposed after Anthesis

• Sowing date: November, 23-30 2016

• Harvesting date: May 10-18, 2017

• Fruiting Efficiency

✓Fruiting efficiency is considered to be a

potential venue for increasing grain

number under stress and non stress

conditions

✓Ratio of grain number to spike dry chaff

weight

Introduction

Results

Materials &Methods

Objectives➢Study genetic source of variations for

fruiting efficiency, grain number, yield and

HI in US soft wheat association panel

under post-anthesis drought stress.

➢ Identify traits and mechanisms determining

genetic variation in fruiting efficiency (FE).

➢Significant genetic variability and good

correlation observed for spike fertility,

harvest index and grain yield traits under

drought conditions.

➢Strong correlations were observed

between FE with harvest index and grain

yield.

➢ Identification of loci involved with

higher FE and related traits in soft wheat

is in progress.

➢Understanding of physiological basis of

increasing fruiting efficiency and the

contributing of different spike

components is in progress.

Bauder, J. 2001. Irrigating with limited water

supplies. Montana State Univ. Comm. Ser.

Montana Hall. Bozeman, MT 59717, USA.

Daryanto, S., Wang, L., & Jacinthe, P.-A. (2016).

Global Synthesis of Drought Effects on

Maize and Wheat Production. PLoS ONE,

11(5).

Gupta, N.K., S. Gupta and A. Kumar. 2001. Effect

of water stress on physiological attributes

and their relationship with growth and yield

of wheat cultivars at different stages. J.

Agron. Crop Sci. 186: 55-62.

Conclusion

Figure 1. Citra and Quincy

Figure 2. Rainfall Citra and Quincy

1.33

3.8

3.2

1.24

3.24

5.95

MARCH APRIL MAY

Rainfall (inch)

Citra Quincy

Acknowledgment

Source

Grain

Yield (g)

Harvest

Index %

Fruiting

Efficiency

Thousand

Grain Wt. (g)

Checks <0.0001 <0.0001 <0.0001 <0.0001

Location <0.0001 <0.0001 <0.0001 <0.0001

Block x Location 0.2177 0.2571 0.6814 0.2845

New x Entry x

Location

0.9095 0.9603 0.2137 0.6763

New x Entry 0.0062 0.0004 0.0052 <0.0001

Table 1. Combined Analysis of Variance

Significant (P<0.05) genetic variations

differences were observed for grain yield,

fruiting efficiency, harvest index, and

thousand grain weight.

Figure 4. Regression Analysis of FE and

related traits

• Plant Material: 300 soft wheat genotypes

• Location: Citra and Quincy , Florida

y = 0.1784x + 4.4899R² = 0.2511

0

20

40

60

80

100

120

140

160

180

0 100 200 300 400 500 600

Fru

it e

ffic

ien

cy

Grain Yield

y = 1.6955x - 2.046R² = 0.2258

0

20

40

60

80

100

120

140

160

180

0 10 20 30 40 50

Fru

it e

ffic

ien

cy

Harvest Index %

y = 0.3772x - 50.54R² = 0.4849

0

100

200

300

400

500

600

0 200 400 600 800 1000 1200 1400

Gra

in Y

ield

Total Dry Matter

y = 8.2651x + 1.1357R² = 0.6803

0

100

200

300

400

500

600

0 10 20 30 40 50

Gra

in Y

ield

Harvest Index

Preliminary Results form two locations

showed R2 values of 0.2511 and 0.2258

for fruiting efficiency with grain yield and

harvest index

Figure 3. Histogram for Lsmeans of FE

PCA for both locations showed positive

correlation between FE and other related

related traits and PC1 accounted for 56.9 %

of variation.

0

20

40

60

80

100

120

140

160

180

Fru

it e

ffic

ien

cy

Genotype