Genetic Engineering -- Digital Portfolio SGP

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    Genetic Engineering

    Scott Chubb.Mr Schurtz

    English 12 AP Period 34 March 2010

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    What isit?

    (LA Genetic Engineeri

    ( )Dykinga

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    , What is DNA exactly?

    ( )DNA

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    :History Mendel and Beyond

    ( )Punnett Square( )Gregor Mendel

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    How doesit work?

    Restriction enzymes and sticky ends

    (Restriction and Ligati

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    Putting itback

    together

    (Recombinant Plasmid

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    What happens next and why should I care?

    (Transformation of competent

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    Products

    ( )Insulin Pen ( )GE Corn

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    ( The Human Genome

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    Concerns and Controversy

    (GM Accepted Map

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    My Application

    .Transformation of E coli

    Purification of a Protein Product

    Placeholder for image once application progresses further

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    Step 1 Grow the E.coli

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    Step 1.5-Pouring NutrientPlate

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    Step 2 E.coli onto plates

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    Class Activities Ideas

    Borrow micropipettes from Mr.Werner, bring in so that studentscan try using the micropipette with

    a dye. Create small activity to simulate

    restriction enzymes have

    students look at a string ofnucleotides and cut at specificsequences

    Borrow vortex from Mr. Werner and

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    Works Cited Designing the Genes of Future Generations. Facts on File. 1998. Todays Science on File. Todays Science.

    Web. 1 Dec. 2009. . Galloway, Evan. Human Genetics: Promise and Controversy. Facts on File. 2009. Facts On File News

    Services. Todays Science. Web. 1 Dec. 2009. .

    Gene Therapy - An Overview.Access Excellence. National Health Museum, 2 Nov. 2009. Web. 1 Dec. 2009..

    Genetic Engineering. Facts on File. Facts on File News Services. Todays Science. Web. 1 Dec. 2009..

    "Genetic Engineering." How Stuff Works. Discovery Communications, 2010. Web. 2Mar. 2010. . Green Fluorescent Protein (GFP) Purification Kit. N.p.: Bio Rad, n.d. Print.

    Human Genome Project Information. U.S. Department of Energy Office of Science, Office of Biological andEnvironmental Research, Human Genome Program, 12 Aug. 2009. Web. 1 Dec. 2009..

    Poirot, Lissa. "Genetic Disorders and Engineering: Implications for PhysicalTherapists." Consumer Health Complete. Vol. 13. 2005. 54-60. Magazine ofPhysical Therapy. Consumer Health Complete. Web. 2 Mar. 2010..

    PGLO Bacterial Transformation Kit. N.p.: Bio Rad, n.d. Print.

    Rhee, Seung Yon. "Gregor Mendel (1822-1884)."Access Excellence. National HealthMuseum, n.d. Web. 2 Mar. 2010. .

    Rubenstein, Irwin. "DNA." World Book Student. N.p., n.d. Web. 3 Mar. 2010.. "Say No to Genetic

    Engineering." Greenpeace. N.p., n.d. Web. 2 Mar. 2010.

    "What is Genetic Engineering?" Mothers for Natural Law. N.p., n.d. Web. 2 Mar.

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    Works Cited Pictures

    DNA. N.d. Photobucket. N.p., n.d. Web. 3 Mar. 2010..

    Dykinga, Jack. Genetic Engineering Wheat. N.d. Wikimedia Commons. N.p., 2 Jan. 2008. Web.3 Mar. 2010. .

    GE Corn. 13 Oct. 2003. Wikimedia Commons. N.p., 14 Nov. 2006. Web. 3 Mar. 2010. .

    GM Accepted Map. 19 Mar. 2009. Wikimedia Commons. N.p., n.d. Web. 3 Mar. 2010. .

    Gregor Mendel. 1928. The History of Biology de Erik Nordenskild. Wikimedia Commons.Web. 3 Mar. 2010. .

    The Human Genome 3. N.d. Flickr. Yahoo, n.d. Web. 3 Mar. 2010..

    Insulin Pen. N.d. Wikimedia Commons. N.p., 20 Sept. 2005. Web. 3 Mar. 2010..

    LA Genetic Engineering. N.d. Photobucket. N.p., n.d. Web. 3 Mar. 2010..

    Punnett Square. 5 May 2007. Wikimedia Commons. N.p., n.d. Web. 3 Mar. 2010..

    Recombinant Plasmids. N.d. Flickr. Yahoo, 27 Oct. 2008. Web. 3 Mar. 2010..

    Restriction and Ligation. N.d. Flickr. Yahoo, 27 Oct. 2008. Web. 3 Mar. 2010.