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AB ANALITICA Srl, Via Svizzera 16 – 35127 PADOVA, tel. +39 049 761698 – e-mail: info@abanalitica.it

www.abanalitica.it

GENEQUALITY AZF Y-TYPE Kit for detection of deletions in the AZF LOCUS by means of Reverse Line Blot The kit GENEQUALITY AZF Y-TYPE (04-18A-20) allows to detect the deletions in the AZF (AZoospermia Factor) locus that are associated with male infertility by means of a single multiplex amplification (PCR) of all examined deletion markers and a Reverse Line Blot.

The tested regions are: • AZFa: sY84, sY86, DFFRY, DBY • between AZFa and AZFb: sY95 • AZFb: sY117, sY125, sY127, sY134 • AZFc: sY254, sY255.

In addition, SRY, which codes for the Testis Determining Factor, and ZFX/Y, used as internal amplification control, are amplified. The analysis of Y chromosome microdeletions is an essential diagnostic tool for the research of male infertility. The long arm of the Y chromosome has been subdivided into three regions called AZFa, AZFb and AZFc which control the correct course of spermatogenesis and often carry deletions in persons with azoospermia and severe oligospermia. Alterations in one or more loci lead to a drastic reduction in number or complete loss of sperm cells. After the Y chromosome had been sequenced and with the understanding of the mechanisms causative of the microdeletions, European Guidelines for molecular diagnosis of y-chromosomal microdeletions were established (Simoni et al., 2004). The product GENEQUALITY AZF Y-TYPE is based on these guidelines.

 Technical Characteristics:

Test  with  CE  IVD  mark  

Amplifica7on  in  SINGLE  MULTIPLEX  (premixed)  

Visualiza7on  using  Reverse  Line  Blot  

Conforms  to  European  Guidelines  

 

________________________________________________________________________________________Rev Oct 2012_____

AB ANALITICA Srl, Via Svizzera 16 – 35127 PADOVA, tel. +39 049 761698 – e-mail: info@abanalitica.it

www.abanalitica.it

 

REFERENCES:

Kamp et al., Human Mol Genet 9, 2563-2572, 2000 Kuroda-Kawaguchi et al., Nature Genetics 29, 279-286, 2001 Repping et al., Am J Hum Genet 71, 906-922, 2002 Simoni et al. International Journal of Andrology, 22: 292-299; 1999. Simoni et al. International Journal of Andrology, 27: 240-249, 2004. Skaletsky et al., Nature, 423, 825-837, 2003 Sun et al., Hum mol Genet, 9, 2291-2296, 2000 Vogt PH. et al., Hum Mol Genet 5, 933-943, 1996

ORDERING INFORMATION:

Code Product PKG Method of visualization

04-18A-20 GENEQUALITY AZF Y-TYPE 20 test Reverse Line Blot

RELATED PRODUCTS:

Code Product PKG Method of visualization 04-23 GENEQUALITY AZF-MX 12 or 25 test Agarose gel

04-24 GENEQUALITY AZF EXTENSION 12 or 25 test Agarose gel

Example analysis of a patient without deletions

Staining control SRY ZFY  sY86 sY84 DFFRY DBY  sY95

sY117 sY125 sY127 sY134

sY254 sY255

AZFa

AZFb

AZFc

1 •  DNA EXTRACTION

FROM BLOOD

2

•  SINGLE MULTIPLEX AMPLIFICATION 2 hours

3 •  HYBRIDIZATION ON STRIP

75 minutes

4 •  INTERPRETATION OF RESULTS