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multiplicación in vitro de vetiver
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Vetiver
multiplication
Elise Pinners
Director, The Vetiver Network International (TVNI), and
Director of Green Cycle Consulting Ltd. (GCC), Kenya
Floricounda Ltd.,
Dakar, Senegal
Different types of
multiplication
1. Bare root slips
2. Flower inflorescence
3. Poly-pots, net-pots
4. Strips
5. Micro-propagation
Plant parts naming,
for different multiplication methods
Tiller: one stem with root
Slip: 2-4 tillers together, basic unit for planting a hedge
Culm: the base of a stem producing new shoots
Clump: a poll of grass
Cutting: a piece if stem including a node where it develops roots (flower inflorescence)
Parts used for
multiplication
Tiller: one stem with root
Slip: min. 2 tillers together, for planting a
hedge (for infrastructure 3-4 tillers/slip)
Culm: the base of a stem producing new
shoots
Clump: a poll of grass
Cutting: a piece if stem including a node
where it develops roots
Vetiver grows new
adaptive roots
above the crown
as it traps eroded
soil
Different multiplication methods
Method Advantage Disadvantage
Bare root
slips
Cheap; little skill & inputs
required; easy transport
Less robust under
hardship
Poly-pots
(plastic,
paper)
Withstanding hardship;
easy transport
More bulky for
transport, more
expensive
Strips For difficult sites (water,
steep slopes); faster
handling & planting; sure
plant distance
Expensive, more
vulnerable during
transport (soil fall
out, strip breaking)
Micro-
propagation
Large scale, fast and
cheap
Needs special
skills & equipment
(lab)
1. Bare root slips
How to speed up rooting
Four steps to propagate cuttings:
1. Prepare cuttings (split, prune)
2. Spray cuttings with 10% water hyacinth solution, or other rooting medium, then cover for 24h with plastic bags
3. OR: dip in clay mud or manure slurry (‘cow tea’)
4. Plant in a good bed. For fast establishment, add manure and/or chemical fertilizer (17:17:17 10g/plant), and start watering daily (check depth of wetness!)
Cowtea dip
New slips are
stimulated to
make new root: in
fertile clay/mud
Vetiver planting material acquired from abandoned
university project area in Darfur
Method to irrigate the
just planted vetiver
Three weeks after planting
3 months after planting
6 months after planting
Nursery near Eldoret (for Kerio
valley project)
2. Flower inflorescence
For use on infrastructure, in harsh,
hostile conditions, requiring
fast and sure establishment Advantages of poly-pots & strips:
i) hardy plants, less affected by
high temperature and low moisture
ii) lower irrigation frequency after planting
iii) can remain on-site longer (than bare root slips) before planting, but: after 1 week increased maintenance costs
Disadvantages:
i) poly-pot plants are more bulky, difficult to transport
ii) more costly (labour, space, pots, soil, etc.)
iii) 4-5 weeks longer in nursery (than bare root slips)
iv) if not bio-degradable, pots are environmentally charging
3. Polypots,
netpots
Propagation in Ethiopia
In low rainfall areas the most efficient and cost effective proved the two-stage production process:
multiplication within nurseries in big poly-tube
multiplication for final planting out in the field using smaller tube.
Cost (Ethiopia)
Following
recommendation
for low rain fall
area: 17 Ethiopian
cents / slip
For high rainfall
and mid- & low
altitude areas: 10
Ethiopian cents /
slip.
Net pots
Easy to plant in rainy season
Cheap (India: + 0.005$/pot)
Advantages:
Easier to transport than polypots
Saves time for planting
You need less soil
(compared to poly-
pots) and plastic
can be re-used
High survival rate
since roots remain
together
4. Strips
PROPAGATION OF VETIVER GRASS HEDGE ROWS
Vetiveria zizanioides (Chrysopogon zizanioides )
Vetiver Grass
Hedge Row
Propagation
by
Yoann Coppin
in
Madagascar
River Bank Stabilisation with VGHR’S
Strip dimensions
Length 50-75 cm
Width 6 cm
Depth 8-10 cm
(in Chile)
ICV45. Micro-propagation
Low cost micro-propagation
procedure developed by College of
Agriculture & Applied Biology,
University of Can Tho. This
procedure uses net-house instead of
the expensive growth chamber.
Produced in net-house conditions
plants were +22% cheaper than
when grown in growth chamber.