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8/13/2019 Fuction of Indgenous Probiotic From Dadih Enterococcus Faecum is-27526 in Improving Nutrional Sta
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Inggrid Surono
Function of Indigenous probiotic fromdadih Enterococcus faeciumIS-27526in improving nutritional status and
suppress diarrhoea
Ingrid S Surono*, Fiona Koestomo, F
Riewpassa, Yulianasari and Netty Harnetty
*SEAMEO TROPMED RCCN University of IndonesiaSalemba Raya 6, Jakarta 10430 INDONESIA
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Dadih, Indonesian traditional
fermented raw buffalo milk of WestSumatra
Enterococcus faeciumEnterococcus faeciumISIS--27526 strain isolated27526 strain isolatedfrom Dadihfrom Dadih possess probiotic propertiespossess probiotic properties
Antagonisms against pathogens
Probiotic Properties
high
viability
acid and bile
toleranceadhesion
colonization
antimutagenic, hypocholesterolemic
Safety in food, clinical safety
processing, shelf life, probiotics stability
Immuno-modulatory
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TRADITIONAL WAY OF DADIH MAKINGTRADITIONAL WAY OF DADIH MAKING
Fresh raw unheated buffalo milk, poured into bamboo tubes
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Diarrhoea in a year globally
4000 milliondiarrhaea cases
1500 millionepisode of diarechildren youngerthan 5
1.8 million die
Every 3 seconds 1 child dies from diarrhoeaEvery 3 seconds 1 child dies from diarrhoea
70% cases related to contaminated food and drink70% cases related to contaminated food and drink
Cause of mortality of Indonesian
children
%
%
%
%
%
%
%
%
%
%
%
%
%
INFANTS UNDER FIVE CHILDREN
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2. Fekal Mikrobiota pada Feses Balita
Fecal Microbiota of children younger than five, before and after supplementationFecal Microbiota of children younger than five, before and after supplementationwith FPC biscuit with probiotic creamwith FPC biscuit with probiotic cream
Profile of fecal microbiota before after delta
1Fecal Aerobic bacteria (cfu/g)Log cfu/g
7.10 0.961.2 x 107
6.42 1.362.5 x 106
0.96 1.65
2Fecal Coliform (cfu/g)Log cfu/g
5.40 0.872.5 x 105
5.50 0.983.1 x 105
0.07 1.28
3Fecal lactic bacteria (cfu/g)Log cfu/g
4.48 3.193.0 x 104
6.35 0.702.2 x 106
1.86 3.41
4Fecal Anaerobic bacteria(cfu/g) Log cfu/g
6.38 0.922.5 x 106
6.29 1.231.9 x 106
0.09 1.15
5Fecal lactic Anaerobic (cfu/g)
Log cfu/g
5.61 0.71
4.0 x 105
6.23 0.99
1.5 x 106 0.62 0.45**
** significantly different before and after (P
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Morbidity of children younger than five subjects during 90days intervention study
Treatment Groups
Normal biscuit FPC biscuit FPC + Probioticcream
Day Day Day
0 30 60 90 0 30 60 90 0 30 60 90
Diarrhoea 5 2 3 1 3 2 4 2 6 4 0 0
High fever - 2 2 0 0 1 1 0 0 2 3 1
Commoncold
1 2 3 3 4 2 2 0 3 3 1 1
Dermatitis 1 1 0 2 0 0 0 0 0 0 0 0
Fever 4 2 2 1 4 2 2 0 5 0 0 0
Cough 5 1 2 1 6 2 2 0 6 0 0 0
Measle 0 0 0 0 1 0 0 0 0 0 0 0
Total 16 11 12 8 18 9 11 2 20 9 4 2
The improvement of Intestinal vili induces by probiotic LIS 10506 and LIS 20506A: Normal Vili ; B: Disruption o of vili caused by LPS E.coli ;
C: the improvement vili by LIS 10506 ; D: the improvement vili by LIS 20506
A B C D
(Reza Ranuh et al., 2008)
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Protein Intestinal's Brush Border
01000020000300004000050000600007000080000
Duodenum
Jejunum
Ileum
Duodenum
Jejunum
Ileum
Duodenum
Jejunum
Ileum
Duodenum
Jejunum
Ileum
Galectin-4 Myosin-1a Occludin ZO-1
concentration
in
ppm
LPS
Placebo
LIS10506
LIS20506
(Reza Ranuh et al., 2008)
Distribusi Ekspresi Protein Brush Border Usus
0
5
10
15
20
25
30
D J I D J I D J I D J I
G M ZO O
Protein Brush Border Usus
TingkatE
kspresi
KONTROL
LPS
LPS + LIS 10506
LPS + LIS 20506
p
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Gambar 21
-0.93
-0.75
-0.16
-0.93
-0.9
-0.82
-0.61
-1.39
-1.1
-1.28
-0.48-0.56
-1.6
-1.4
-1.2
-1
-0.8
-0.6
-0.4
-0.2
0
0 30 60 90
Days
Zscores
Biskuit Biskuit KPI Biskuit KPI+Probiotik
Z scores (weight/height)
Normal biscuit FPC biscuit FPC + probiotic cream
Effect of supplementation on nutritional status of
children less than five
Z scores
Before intervention After intervention
Normalbiscuit(n=35)
FPCbiscuit(n=32)
FPC +Probiotic
cream (n=35)
Biskuit(n=35)
Biskuit KPI(n=32)
Biskuit KPI+ Probiotik
(n=35)
n % n % n % n % n % n %
Weight/age
Normalunderweight
1223
34.365.7
626
18.881.2
431
11.488.6
2015
57.142.0
1418
43.856.2
1520
42.957.1
Height/age
NormalStunted
1421
4060
1814
56.243.8
1421
4060
1421
4060
1913
59.440.6
1916
54.345.7
Weight/height
NormalWasted
314
88.611.4
257
78.121.9
332
91.45.8
341
97.12.9
311
96.93.1
350
1000
Note : weight/age: Normal -2
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Human Study in Teluk NagaPublic Health Center -
Tangerang
Randomised Placebo Control Trial, Pre-post study
Two groups, n = 40 subjects, 90 days supplementationProbiotic E. faecium IS-27526 108 cfu/day
0.2200.231
0.2740.267
0.000
0.400
0.800
Placebo Probiotic
Group
Consentration(mg/ml)
0 d 90 d
Total Serum IgA
Total serum IgA (mg/ml), before and after intervention
Placebo : maltodextrinProbiotic : 108 cfu/day Enterococcus faeciumIS-27526
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So, which bacteria did you eat today?