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FRAC-Brasil activitiesFRAC-Brasil activities
FRAC BrasilFRAC Brasil
1999 – Official start in Jaguariúna, São Paulo1999 – Official start in Jaguariúna, São Paulo First Board Committee:First Board Committee:
– Chairperson:Chairperson: Eric Guicherit (Novartis) Eric Guicherit (Novartis)– Vice Chairperson:Vice Chairperson: Alberto Marçon (Du Pont) Alberto Marçon (Du Pont)– Secretary:Secretary: Sergio Paiva (Zeneca) Sergio Paiva (Zeneca)– Treasury:Treasury: Alexandre Frateschi (Dow) Alexandre Frateschi (Dow)
FRAC BrasilFRAC Brasil
2009–20011 Board Committee2009–20011 Board Committee– Chairperson:Chairperson: Carlos Medeiros (Basf) Carlos Medeiros (Basf)– Vice Chairperson:Vice Chairperson: Rafael Pereira (Bayer) Rafael Pereira (Bayer)– Secretary:Secretary: Elisa Basso (Syngenta) Elisa Basso (Syngenta)– Treasury:Treasury: Olavo Correa (DowAgroSciences) Olavo Correa (DowAgroSciences)
MembershipMembership
R&D members from 12 companies (SINDAG R&D members from 12 companies (SINDAG members)members)– Arysta Arysta – Basf Basf – Bayer Bayer – CheminovaCheminova– DowAgroSciencesDowAgroSciences– DuPontDuPont– FMCFMC– IharaBrasIharaBras– Milenia Milenia – NuFarmNuFarm– Sipcam-Isagro Sipcam-Isagro – SyngentaSyngenta
““Modus operandi”Modus operandi”
Meetings:Meetings: two official per year (during Regional and two official per year (during Regional and National Plant Pathology Congress)National Plant Pathology Congress)– approve activities and projectsapprove activities and projects– approve/decision on expensesapprove/decision on expenses– each member has 2 representatives but only one votes per each member has 2 representatives but only one votes per
CompanyCompany
Board committee:Board committee: eligible every 2 years (in August) eligible every 2 years (in August)
Annual fee:Annual fee: paid by members to support group paid by members to support group activities (website, courses, books, etc.)activities (website, courses, books, etc.)
Projects:Projects: paid by FRAC-Brasil (25%) and Companies paid by FRAC-Brasil (25%) and Companies (75%).(75%).
Educational ProjectsEducational Projects
Training sessions for technicians, dealers, Training sessions for technicians, dealers, undergraduate and graduate students;undergraduate and graduate students;
Presentations on Research Institutions; Presentations on Research Institutions;
Web Site (www.frac-brasil.org.br);Web Site (www.frac-brasil.org.br);
FRAC International's Monograph (1, 2 and 3): FRAC International's Monograph (1, 2 and 3): translation to Portuguese finalized and in translation to Portuguese finalized and in diagramming phase;diagramming phase;
Print of Mode of Actions FRAC International Print of Mode of Actions FRAC International Poster's and distribution to Plant Pathologist Poster's and distribution to Plant Pathologist Researchers: ongoing.Researchers: ongoing.
Monitoring sensitivity of Monitoring sensitivity of Ramularia areolaRamularia areola from cotton to QoIs, triazoles and from cotton to QoIs, triazoles and benzimidazolesbenzimidazoles
Status:Status: Collaborators:Collaborators:
– The Committee agreed that will be interesting The Committee agreed that will be interesting looking for another researcher.looking for another researcher.
Coordinators:Coordinators:– Elisa Basso (Syngenta)Elisa Basso (Syngenta)– Reginaldo Sene (Cheminova)Reginaldo Sene (Cheminova)
Monitoring sensitivity of Monitoring sensitivity of Puccinia triticinaPuccinia triticina from wheat to QoI and triazolesfrom wheat to QoI and triazoles
Status: Status: ongoingongoing CollaboratorsCollaborators
– Erlei Melo Reis: Universidade de Passo Fundo Erlei Melo Reis: Universidade de Passo Fundo (UPF)(UPF)
– Amarilis: OR Melhoramento de Sementes Ltda.Amarilis: OR Melhoramento de Sementes Ltda.
Coordinators:Coordinators:– Alisson Celmer (Milenia)Alisson Celmer (Milenia)– Marssal Tamagnone (Sipcam Isagro) Marssal Tamagnone (Sipcam Isagro)
New results is being discussedNew results is being discussed
Monitoring sensitivity of Monitoring sensitivity of Phakopsora Phakopsora pachyrhizipachyrhizi from soybean to QoI and triazoles from soybean to QoI and triazoles productsproducts
Collaborators:Collaborators:– The Committee agreed that was interesting The Committee agreed that was interesting
change the researchers.change the researchers.– FRAC-Brasil is finalizing agreements with the FRAC-Brasil is finalizing agreements with the
researchers. They will be announced soon.researchers. They will be announced soon.
Coordinators:Coordinators:– Carlos Medeiros (Basf)Carlos Medeiros (Basf)– Christian Scherb (Nufarm) Christian Scherb (Nufarm) – Rafael Pereira (Bayer)Rafael Pereira (Bayer)
Results:Results: Erlei Melo Reis: University of Passo Fundo (UPF)Erlei Melo Reis: University of Passo Fundo (UPF)
2007-2008 season: 8 samples: 2007-2008 season: 8 samples: – RS:RS: Passo Fundo; Passo Fundo; GO: GO: Jataí; Santa Helena; Rio Verde; Jataí; Santa Helena; Rio Verde; SP:SP:
Holambra; Paulínia; Holambra; Paulínia; MS: MS: Chapadão do Sul; Chapadão do Sul; MT: MT: RondonópolisRondonópolis
2008-2009 season: 19 samples 2008-2009 season: 19 samples – BA: BA: Luis Eduardo Magalhães (2);Luis Eduardo Magalhães (2); GO: GO: Senador Canedo; Senador Canedo;
Goiânia (2); Goiânia (2); MS: MS: Dourados; Ponta Porã (2); Dourados; Ponta Porã (2); MT: MT: Campo Campo Verde (2); Chapadão do Céu; Diamantina; Tangará da Verde (2); Chapadão do Céu; Diamantina; Tangará da Serra; Serra; PR: PR: Arapoti, Campo Mourão (2); Londrina, Ponta Arapoti, Campo Mourão (2); Londrina, Ponta Grossa; Grossa; SP: SP: HolambraHolambra
Sensibility Monitoring of Sensibility Monitoring of Phakopsora Phakopsora pachyrhizipachyrhizi from soybean to QoI and triazoles from soybean to QoI and triazoles productsproducts
Effect of substrata on germination of urediniospores and on Effect of substrata on germination of urediniospores and on length of germ tube of length of germ tube of Phakopsora pachyrhiziPhakopsora pachyrhizi (time of (time of exhibition 6h; dark: 22°C)exhibition 6h; dark: 22°C)
Effect of temperature on urediniosporos germination of Effect of temperature on urediniosporos germination of Phakopsora pachyrhizi Phakopsora pachyrhizi (time of exhibition 6h; dark; agar- (time of exhibition 6h; dark; agar- water)water)
Experiment 1
y = -0,3079x2 + 13,456x - 89,886R² = 0,90 p<0,0001
0
20
40
60
80
100
0 5 10 15 20 25 30 35 40
Ger
min
ação
(%)
Temperatura (� C) Experiment 2
y = -0,3482x2 + 15,521x - 106,55R² = 0,90 p<0,0001
0
20
40
60
80
100
0 5 10 15 20 25 30 35 40
Ger
min
ação
(%)
Temperatura (� C)
Effect of temperature on urediniospores length of germ Effect of temperature on urediniospores length of germ tube of tube of Phakopsora pachyrhiziPhakopsora pachyrhizi (time of exhibition 6h; dark; (time of exhibition 6h; dark; agar-water)agar-water)
Experiment 1
y = -0,5536x2 + 23,746x - 122,3R² = 0,95 p<0,0001
0
25
50
75
100
125
150
0 5 10 15 20 25 30 35 40
Com
prim
ento
tubo
ger
min
ativ
o (µ
m)
Temperatura (� C) Experiment 2
y = -0,6826x2 + 30,173x - 196,73R² = 0,96 p<0,0001
0
25
50
75
100
125
150
0 5 10 15 20 25 30 35 40
Com
prim
ento
tubo
ger
min
ativ
o (µ
m)
Temperatura (� C)
Effect of time of exhibition on germination of Effect of time of exhibition on germination of urediniospores of urediniospores of Phakopsora pachyrhiziPhakopsora pachyrhizi (agar-water; dark; (agar-water; dark; 22°C)22°C)
Experiment 1
y = -0,0451x2 + 2,467x + 28,958R² = 0,51 p=0,002
0
10
20
30
40
50
60
70
80
0 4 8 12 16 20 24 28 32 36 40 44
Ger
min
ação
(%
)
Tempo (h)
Experiment 2
y = -0,0418x2 + 1,9484x + 27,325R² = 0,21 p=0,0045
0
10
20
30
40
50
60
70
0 4 8 12 16 20 24 28 32 36 40 44
Ger
min
ação
(%)
Tempo (h)
Effect of time of exhibition on germination of Effect of time of exhibition on germination of urediniospores of urediniospores of Phakopsora pachyrhiziPhakopsora pachyrhizi (agar-water; dark; (agar-water; dark; 22°C)22°C)
Experiment 1
y = -2,2188x2 + 28,305x - 25,701R² = 0,94 p<0,0001
0
10
20
30
40
50
60
70
80
0 1 2 3 4 5 6 7 8 9
Ger
min
ação
(%)
Tempo (h)
Experiment 2
y = -2,1845x2 + 28,095x - 13,911R² = 0,89 p<0,0001
0
10
20
30
40
50
60
70
80
90
0 1 2 3 4 5 6 7 8 9
Ger
min
ação
(%)
Tempo (h)
Effect of time of exhibition on length of germ tube of Effect of time of exhibition on length of germ tube of Phakopsora pachyrhiziPhakopsora pachyrhizi (agar-water; dark; 22°C) (agar-water; dark; 22°C)
Experiment 1
y = -0,4929x2 + 37,176x - 48,252R² = 0,93 p<0,0001
0
100
200
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400
500
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700
800
900
0 4 8 12 16 20 24 28 32 36 40 44
Com
prim
ento
tubo
ger
min
ativ
o (µ
m)
Tempo (h)
Experiment 2
y = -0,5101x2 + 38,485x - 89,301R² = 0,95 p<0,0001
0
100
200
300
400
500
600
700
800
0 4 8 12 16 20 24 28 32 36 40 44Com
prim
ento
tubo
ger
min
ativ
o (µ
m)
Tempo (h)
Effect of time of exhibition on length of germ tube of Effect of time of exhibition on length of germ tube of Phakopsora pachyrhiziPhakopsora pachyrhizi (agar-water; dark; 22°C) (agar-water; dark; 22°C)
Experiment 1
y = 44,25x - 60,967R² = 0,97 p<0,0001
0
50
100
150
200
250
300
350
0 1 2 3 4 5 6 7 8 9
Com
prim
ento
tubo
ger
min
ativ
o (µ
m)
Tempo (h)
Experiment 2
y = 45,181x - 59,033R² = 0,98 p<0,0001
0
50
100
150
200
250
300
350
400
0 1 2 3 4 5 6 7 8 9
Com
prim
ento
tubo
ger
min
ativ
o (µ
m)
Tempo (h)
Effect of photoperiod and length of germ tube of Effect of photoperiod and length of germ tube of Phakopsora pachyrhiziPhakopsora pachyrhizi (agar-water; dark; 22°C; D=dark; L (agar-water; dark; 22°C; D=dark; L = light)= light)
b
a
b
cb
a
b
c0
10
20
30
40
50
60
70
3E+3L 6E 3L+3E 6L
Ger
min
ação
(%)
Fotoperíodo (h)
Experimento 1
Experimento 2
exp. 1: C.V. 18,7%exp. 2: C.V. 15,4%
b
a
bc
b
a
bb
0
20
40
60
80
100
120
140
160
180
3E+3L 6E 3L+3E 6L
Com
prim
ento
tubo
ger
min
ativ
o (µ
m)
Fotoperíodo (h)
Experimento 1
Experimento 2
exp. 1: C.V. 6,5%exp. 2: C.V. 14,0%
Effect of Effect of photoperiod and length of germ photoperiod and length of germ tube of Phakopsora pachyrhizi (ágar-water; tube of Phakopsora pachyrhizi (ágar-water; dark; 22°C; D=dark; L = light)dark; 22°C; D=dark; L = light)
e e
c
b
b
a
b
c
decd cd
e
gg fg
cdefbcde
a
b
bc bcd
defg efgfg
0
10
20
30
40
50
60
70
1E+5
L
2E+4
L
3E+3
L
4E+2
L
5E+1
L 6E
1L+5
E
2L+4
E
3L+3
E
4L+2
E
5L+1
E 6L
Ger
min
ação
(%)
Fotoperíodo (h)
Experimento 1
Experimento 2
exp. 1: C.V. 13,3%exp. 2: C.V. 13,4%
f ef
d
bc
b
a
c c
de
def
dedef
bcd bcdbc
a a a a
bbc
cd cdd
0
20
40
60
80
100
120
140
160
180
1E+5
L
2E+4
L
3E+3
L
4E+2
L
5E+1
L
6E
1L+5
E
2L+4
E
3L+3
E
4L+2
E
5L+1
E
6L
Com
prim
ento
tubo
ger
min
ativ
o (µ
m)
Fotoperíodo (h)
Experimento 1
Experimento 2
exp. 1: C.V. 9,9%exp. 2: C.V. 9,5%
Inhibitory concentration (mgL-1) of QoI fungicides to Inhibitory concentration (mgL-1) of QoI fungicides to reduce 50% of uredospores of reduce 50% of uredospores of Phakopsora pachyrhiziPhakopsora pachyrhizi, in , in vitro, (agar – water) 22°; time of exhibition 6h; absence of vitro, (agar – water) 22°; time of exhibition 6h; absence of light.light.
Inhibitory concentration (mgL-1) of QoI fungicides to Inhibitory concentration (mgL-1) of QoI fungicides to reduce 50% of growth of germ tubes from urediniospores reduce 50% of growth of germ tubes from urediniospores of of Phakopsora pachyrhiziPhakopsora pachyrhizi in vitro (ágar – water) 22°; time in vitro (ágar – water) 22°; time of exhibition 6h; absence of light).of exhibition 6h; absence of light).
Inhibitory concentration (mgL-1) of QoI fungicides to Inhibitory concentration (mgL-1) of QoI fungicides to reduce 50% of growth of number of lesions per cmreduce 50% of growth of number of lesions per cm22 from from Phakopsora pachyrhiziPhakopsora pachyrhizi, sensitive isolate, in detached , sensitive isolate, in detached soybean leafs.soybean leafs.
Inhibitory concentration (mgL-1) of QoI fungicides to Inhibitory concentration (mgL-1) of QoI fungicides to reduce 50% of growth of number of pustules per cm2 from reduce 50% of growth of number of pustules per cm2 from Phakopsora pachyrhiziPhakopsora pachyrhizi, sensitive isolate, in detached , sensitive isolate, in detached soybean leafs.soybean leafs.
Inhibitory concentration (mgL-1) of triazoles fungicides to Inhibitory concentration (mgL-1) of triazoles fungicides to reduce 50% of growth of number of lesions per cmreduce 50% of growth of number of lesions per cm22 from from Phakopsora pachyrhiziPhakopsora pachyrhizi, sensitive isolate, in detached , sensitive isolate, in detached soybean leafs.soybean leafs.
Inhibitory concentration (mgL-1) of triazoles fungicides to Inhibitory concentration (mgL-1) of triazoles fungicides to reduce 50% of growth of number of pustules per cm2 from reduce 50% of growth of number of pustules per cm2 from Phakopsora pachyrhiziPhakopsora pachyrhizi, sensitive isolate, in detached , sensitive isolate, in detached soybean leafs.soybean leafs.
Sensibility Monitoring of Sensibility Monitoring of Phakopsora Phakopsora pachyrhizipachyrhizi from soybean to QoI and triazoles from soybean to QoI and triazoles productsproducts
Results:Results: Ricardo Balardin: University of Santa Maria (UFSM)Ricardo Balardin: University of Santa Maria (UFSM)
2007-2008 season: 5 samples2007-2008 season: 5 samples– SP: Est. Experimental Holambra; Paulínia (2)– MT: Rondonópolis; Lucas do Rio Verde
2008-2009 season: 12 samples2008-2009 season: 12 samples– BA: Luis Eduardo Magalhães (2)BA: Luis Eduardo Magalhães (2)– GO: Chapadão do Céu; Goiânia; Senador CanedoGO: Chapadão do Céu; Goiânia; Senador Canedo– MS: MS: DouradosDourados– MT: Campo Verde; Lucas do Rio Verde MT: Campo Verde; Lucas do Rio Verde – PR: Arapoti; Londrina; Ponta Grossa PR: Arapoti; Londrina; Ponta Grossa – SP: SP: HolambraHolambra
Fungicides and letal dose capable to reduce Fungicides and letal dose capable to reduce 50% of uredias. Santa Maria 2009.50% of uredias. Santa Maria 2009.
Fungicides and letal dose capable to reduce Fungicides and letal dose capable to reduce 50% of uredias. Santa Maria 2009.50% of uredias. Santa Maria 2009.
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