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INTER-OFFICE MEMORANDUMSubject: Glucose Isomerase Report(Period
Covering November 24 toDecember 5, 1969)
To: Mr . Edward A . Vassallo
r
Date: De ce mber 10, 1969
The glucose isomerase activity has been raised in the shake
flaskstudies to 600 to 700 uU per ml but the activity in the
30-gallon fer-mentors is still about 500 uU per ml . The reasons
for this differencein activity have not been resolved . However,
preliminary data indicatethat a minimal medium will yield
approximately the same enzyme yieldas the more complex normal
production medium .Since this culture of Arthrobacter exhibits
cellular differentiationin the different stages of its growth cycle
and the cells contain greaterenzymatic activity in the rod form,
the cell morphology of the finalinoculum may greatly influence the
final enzyme yield . Therefore, themethods for preparation of the
final inoculum are being reviewed with special
emphasis on the time,level,and culture morphology .The pilot
plant enzymatic isomerization (1,000 gallons) has beenscheduled for
December 14 or 15 in Cedar Rapids . Appropriate personnel
from the Research Department will carry the cells to Cedar
Rapids and,with the assistance of the Penick and Ford personnel,
monitor the conversion .The percent fructose during the conversion
will be monitored by an opticalrotation procedure and periodic
samples will be returned to Winston-Salem
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2
the protein released from the cells constituted only 18% of the
totalcellular protein. The level of macromolecular contamination
from theArthrobacter cells in pure glucose was about one-fifth of
the macro-molecular level that was observed in the high DE syrup
.Freundlich decolorization isotherms were obtained in bothuntreated
and dialysed enzymatically isomerized high DE syrup . Theresults
indicate that the macromolecular contamination does not increasethe
color bodies but does reduce the efficiency of the carbon to
removethese colored impurities .
Charles G . Pheil
CGP :vbl
Cc: Mr. D . S . PeoplesDr. Murray SenkusDr. R. E . FarrarDr. E .
D . NielsonDr. R . E . BrouillardDr . E . C . RitchellMr . W . C .
BlackMr . Claude SawyerDr. D . J . LartigueDr. J . L . Ayres .
L-PY . Paul BenkoMr . L . E . HayesDr. Chin K . LeeMiss Margaret
E . Long
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FREDERICK POST COMPANY901TGM MILLIMETER t0%10 PER CM
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