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2021-1Please add Test ID here
Proficiencytesting@forensicfoundationsForensic Biology
Biological examination and DNA - 1Sexual Assault
2021-1
Thank you for participating in this Proficiency Test. We hope that you find this test useful and welcome any feedback which can be used to improve the design of further tests.
In addition to this exercise being a test of your laboratory procedures using controlled items, we also anticipate that it will enable participants to evaluate the quality of their analytical results against those from other laboratories and observe how other laboratories express their opinions or advise for their clients. To enable this, we request that participants submit the following:
An outline of the methodology used; and Their opinion in the format that they would provide to the court.
Forensic Foundations’ Proficiency Tests are designed to test the end-to-end forensic examination process. The AS 5388 and the ISO 21043 series of Standards describe the forensic examination process from collection to reporting. This figure1 illustrates the inter-relatedness of all steps in this process and was used as the basis of the Australian Standards’ development. The figure is also used as the basis of the development of Forensic Foundations’ Proficiency Tests. Thus, all Forensic Foundations’ Proficiency Tests commence with item collection and/or receipt and includes all the subsequent examination / analysis steps, culminating in the reporting of results, the process therefore reflects actual forensic casework.
Attached you will find the case ‘Examination Request and Item Submission’ form and the test commences with the receipt of the items followed by your routine processes - item description, examination, analysis and interpretation. The information submitted to the laboratory on the examination request form will direct what testing needs to be undertaken. Please use the attached results sheets. Additional pages may be added if required. An electronic copy of the results sheet can be downloaded from https://www.forensicfoundations.com.au/download/ The results sheets should be returned to Forensic Foundations by 28th May 2021. Hardcopy can be returned to PO Box 2279, Ringwood, Victoria, 3134, Australia or a soft copy can be uploaded to https://www.forensicfoundations.com.au/upload/
To meet the requirements of the National Privacy Principles, DNA Profiles of the donors must not be permanently uploaded onto a DNA database.
Qualitative feedback will be provided to participants. Feedback will be both participant-specific (i.e., whether a particular laboratory “got the right answer”) and group specific (e.g., which techniques seemed to perform better than others).
Following the conclusion of the testing participants will be advised of the expected results and information regarding the production of the test.
1James Robertson, Karl Kent & Linzi Wilson-Wilde (2013) The Development of a Core Forensic Standards Framework for Australia, Forensic Science Policy & Management: An International Journal, 4:3-4, 59-67
2021-1Please add Test ID here
Forensic Biology 2021-1 - Results Sheets
EXAMINATION REQUEST AND ITEM SUBMISSION
EASTERN AUSTRALIAN
POLICE SERVICEOFFENCE: Sexual Assault
DATE OF OFFENCE Friday 19th February 2021
BRIEF STATEMENT OF FACTSMs Megan Cook presented at the emergency department of The Eastern Metropolitan Hospital. She reported that she had been sexually assaulted while at a night club. She immediately replaced her clothing and walked to the hospital. She is not sure who the assailants were but believes them to be friends of friends.
Ms Cook was examined by a forensic medical officer and swabs were taken for testing.
ITEM SUBMITTED FOR EXAMINATIONItem 1 – Reference sample – suspect 1 – Robin PikeItem 2 – Reference sample – suspect 2 – Lee FieldItem 3 – Reference sample – suspect 3 – Nick JacksonItem 4 – Reference sample – suspect 4 – Tony WhiteItem 5 – Medical samples comprising:
Reference sample from the complainant High vaginal swab collected from the complainant Low vaginal swab collected from the complainant
EXAMINATION REQUESTED
An examination of the medical samples collected from the complainant for the presence of biological material. A comparison of any biological material located on the medical samples with reference samples obtained from the four suspects.An evaluation of the weight which can be assigned to any match, if any match is found.
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2021-1Please add Test ID hereForensic Biology 2021-1 - Results Sheets
Continuity, receipt and description of items
Item 1
Yes/No CommentsSubmission to the laboratory undertaken correctly, in accordance with laboratory proceduresSample description corresponds to accompanying paperworkSecurity seals intact
Item fully described in case notesItem recorded by means of photographyDescription of any deviations from the expected
Item 2
Yes/No CommentsSubmission to the laboratory correctly recorded, in accordance with laboratory proceduresSample description corresponds to accompanying paperworkSecurity seals intact
Item fully described in case notesItem recorded by means of photographyDescription of any deviations from the expected
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2021-1Please add Test ID hereForensic Biology 2021-1 - Results Sheets
Item 3
Yes/No CommentsSubmission to the laboratory correctly recorded, in accordance with laboratory proceduresSample description corresponds to accompanying paperworkSecurity seals intact
Item fully described in case notes
Item recorded by means of photographyDescription of any deviations from the expected
Item 4
Yes/No CommentsSubmission to the laboratory correctly recorded, in accordance with laboratory proceduresSample description corresponds to accompanying paperworkSecurity seals intact
Item fully described in case notes
Item recorded by means of photographyDescription of any deviations from the expected
Item 5
Yes/No CommentsSubmission to the laboratory correctly recorded, in accordance with laboratory proceduresSample description corresponds to accompanying paperworkSecurity seals intact
Item fully described in case notes
Item recorded by means of photographyDescription of any deviations from the expected
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2021-1Please add Test ID hereForensic Biology 2021-1 - Results Sheets
Examination /Analysis
Items 1- 4
Used in this examination Y/N
Result Comments
Presumptive testingAlternative light sourcesLight microscopySpecialised microscopyHemastixKastel MeyerLeuco Malachite GreenAcid Phosphatase / BrentamineProstate specific p30 testPhosphatesmo KMPhadebasOther – please specifyConfirmatory testingMicroscopy
Christmas tree stainHematoxylin & Eosin
RSID SemenRSID SalivaRSID BloodABACard HemaTraceHemochromogen TestRSID UrineOther – please specifyMethod of subsampling (if required)Cutting / excisionTape liftsSwabbingMethod used for DNA extractione.g. Chelex, organic, commercial kit (please specify), differentialMethod used for DNA quantificatione.g. commercial kit (please specify), gel electrophoresis, spectroscopy Amplification System(s) usede.g. commercial kit (please specify), in-house method (please specify)Electrophoresis and detection methode.g. DNA sequencer & associated software (please specify), agarose / acrylamide gel & staining
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2021-1Please add Test ID hereForensic Biology 2021-1 - Results Sheets
Item 5
Used in this examination Y/N
Result Comments
Presumptive testingAlternative light sourcesLight microscopySpecialised microscopyHemastixKastel MeyerLeuco Malachite GreenAcid Phosphatase / BrentamineProstate specific p30 testPhosphatesmo KMPhadebasOther – please specifyConfirmatory testingMicroscopy
Christmas tree stainHematoxylin & Eosin
RSID SemenRSID SalivaRSID BloodABACard HemaTraceHemochromogen TestRSID UrineOther – please specifyMethod of subsampling (if required)Cutting / excisionTape liftsSwabbingMethod used for DNA extractione.g. Chelex, organic, commercial kit (please specify), differentialMethod used for DNA quantificatione.g. commercial kit (please specify), gel electrophoresis, spectroscopy Amplification System(s) usede.g. commercial kit (please specify), in-house method (please specify)Electrophoresis and detection methode.g. DNA sequencer & associated software (please specify), agarose / acrylamide gel & staining
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2021-1Please add Test ID hereForensic Biology 2021-1 - Results Sheets
Comments with respect to examination / analysis…………………………………………………………………………………………………
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2021-1Please add Test ID hereForensic Biology 2021-1 - Results Sheets
DNA Results
To meet the requirements of the National Privacy Principles, DNA Profiles of the donors must not be permanently uploaded onto a DNA database.
Please enter DNA typing results in the tables below Include any additional loci Copy these pages as many times as you require to include all the DNA typing
results
Autosomal
Sample
Locus
D1S1656
D2S441
D2S1338
D3S1358
D3S3045
D4S2366
D5S818
D5S2500
D6S477
D6S1043
D7S820
D7S3048
D8S1132
D8S1179
D9S925
D10S1248
D10S1435
D11S2368
D12S391
D13S317
D13S325
D14S608
D15S659
D16S539
D17S1290
D18S51
D18S535
D19S433
D19S253
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2021-1Please add Test ID hereForensic Biology 2021-1 - Results Sheets
Sample
Locus
D20S470
D21S11
D21S1270
D22S1045
D22GATA19
8B05
CSF1PO
FGA
Penta D
Penta E
QS1
QS2
SE33
TH01
TPOX
vWA
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2021-1Please add Test ID hereForensic Biology 2021-1 - Results Sheets
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2021-1Please add Test ID hereForensic Biology 2021-1 - Results Sheets
Non-autosomal
Sample
Locus
AMEL
Yindel
DYS19
DYS385 a/b
DYS389-I
DYS389-II
DYS390
DYS391
DYS392
DYS393
DYS437
DYS438
DYS439
DYS456
DYS448
DYS449
DYS460
DYS481
DYS518
DYS533
DYS570
DYS576
DYS627
DYS635
DYS3891
DYS38911
YGATAH4
DYF387S1
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2021-1Please add Test ID hereForensic Biology 2021-1 - Results Sheets
Case analysis , Interpretation and Conclusions(please include the wording you would use in your court report)…………………………………………………………………………………………………
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2021-1Please add Test ID hereForensic Biology 2021-1 - Results Sheets
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2021-1Please add Test ID hereForensic Biology 2021-1 - Results Sheets
General Comments…………………………………………………………………………………………………
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ACCREDITATION RELEASE STATEMENTForensic Foundations will submit your Proficiency Test results directly to your accreditation body, if this is a requirement of your accreditation. Please select one of the options below if you wish for your data to be submitted on your behalf. We do not wish for our data to be submitted to an accreditation body. Please forward our Proficiency Test results to our accreditation body. Our details are given below
Accreditation body (e.g. ANAB, CNAS)Accreditation Certificate numberAuthorised laboratory contact person (Please print)Contact details of contact personLaboratory / facility
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