MMP9 (N-terminal)Polyclonal ANTIBODYCatalog Number:10375-2-AP Featured Product 331 Publications

For Research Use Only

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Basic Information Catalog Number:10375-2-AP

Size:41 μg/150 μl

Source:Rabbit

Isotype:IgG

Purification Method:Antigen affinity purification

Immunogen Catalog Number:AG0552

GenBank Accession Number:BC006093

GeneID (NCBI):4318

Full Name:matrix metallopeptidase 9(gelatinase B, 92kDa gelatinase,92kDa type IV collagenase)

Calculated MW:707aa,78 kDa

Observed MW:67 kDa, 92 kDa

Recommended Dilutions:WB 1:300-1:1000 IP 0.5-4.0 ug for IP and 1:200-1:1000for WBIHC 1:50-1:500 IF 1:20-1:200

Applications Tested Applications:IF, IHC, IP, WB, ELISA

Cited Applications:CoIP, IF, IHC, WB

Species Specificity:human, mouse, rat

Cited Species:Astragalus membranaceus, hamster, human, mouse,rabbit, rat, swine

Note-IHC: suggested antigen retrievalwith TE buffer pH 9.0; (*) Alternatively,antigen retrieval may be performedwith citrate buffer pH 6.0

Positive Controls:

WB : HepG2 cells; mouse heart tissue, A375 cells,Jurkat cells, HUVEC cells, Raji cells, rat brain tissue

IP : Jurkat cells;

IHC : human breast cancer tissue; human cervicalcancer tissue, human tonsillitis tissue, mouse hearttissue

IF : HeLa cells;

Background Information Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix innormal physiological processes, such as embryonic development, reproduction, tissue remodeling, and diseaseprocesses, such as arthritis or metastasis. Most MMP's are secreted as inactive proproteins which are activated whencleaved by extracellular proteinases. Matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IVcollagenase) (MMP9, synonyms: GELB, CLG4B) degrades collagens type IV and V. Studies in rhesus monkeys suggestthat MMP9 is involved in IL-8-induced mobilization hematopoietic progenitor cells from bone marrow, and murinestudies suggest a role in tumor-associated tissue remodeling. The pro-MMP9 is 92 kDa, and it can be detected aprocessed form of 68 kDa. This protein can exist as a dimer of 180 kDa (PMID:7492685).

Notable Publications Author Pubmed ID Journal Application

Samana Batool 30274346 Int J Mol Sci WB

Yangke Cai 29097832 Dis Markers WB

Taishu Wang 30268747 Int J Biochem Cell Biol WB

Storage Storage:Store at -20ºC. Stable for one year after shipment.Storage Buffer:PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Aliquoting is unnecessary for -20ºC storage

T: 1 (888) 4PTGLAB (1-888-478-4522) (toll freein USA), or 1(312) 455-8498 (outside USA)

E: proteintech@ptglab.com W: ptglab.com

For technical support and original validation data for this product please contact: This product is exclusively available under ProteintechGroup brand and is not available to purchase from anyother manufacturer.

Selected Validation Data

Various lysates were subjected to SDS PAGEfollowed by western blot with 10375-2-AP (MMP9(N-terminal) antibody) at dilution of 1:1000incubated at room temperature for 1.5 hours

IP Result of anti-MMP9 (N-terminal) (IP:10375-2-AP,3ug; Detection:10375-2-AP 1:300) with Jurkat cellslysate 4000ug.

WB result of MMP9 antibody (10375-2-AP, 1:600)with si-control and si-MMP9 transfected Jurkatcells.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using10375-2-AP (MMP9 (N-terminal) antibody) atdilution of 1:200 (under 10x lens) heat mediatedantigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using10375-2-AP (MMP9 (N-terminal) antibody) atdilution of 1:200 (under 40x lens) heat mediatedantigen retrieved with Tris-EDTA buffer(pH9).

Immunofluorescent analysis of HeLa cells using10375-2-AP(MMP9 (N-terminal) antibody) atdilution of 1:50 and Alexa Fluor 488-conjugatedAffiniPure Goat Anti-Rabbit IgG(H+L)