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For Capillary Electrophoresis & Fragment Analysis
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√ NanoPOP™ Genetic Analyzer Polymers
√ BrightDye® Terminator Cycle Sequencing Kit
√ FastGene® DyeTerminator Removal kit
√ D-Pure™ DyeTerminator Removal Kit
√ Fluorescent DNA Size Standards
√ 310 Capillaries
NanoPOP™ Genetic Analyzer Polymers
√ High resolution & performance √ Better stability & shelf life √ Compatible with all ABI Genetic Analyzers
IntroductionNanoPOP-4™, NanoPOP-6™ & NanoPOP-7™ are separation ma-trixes, newly formulated from na-noparticles. These new matrixes, designed for ABI genetic analyz-ers, have excellent coating and separating ability with all differ-ent applications. All NanoPOP polymers can be used without any change in run protocols, condi-tions or spectral calibrations. De-veloped with the latest polymer chemistry technology, NanoPOP has a increased stability and shelf life.
Save up to 50% on polymers for your ABI Genetic Analyzer without compromise!
CompatabilityNanoPOP-4™ is compatible with CE 10X running buffer. Nano- POP-7™ and NanoPOP-6™ are compatible with NanoPOP 10X running buffer. CE 10X running buffer is also compatible with ABI’s POP-4™, POP-6™ & POP-7™.
Name Description P/N
NanoPOP-4 (3130/3130XL) 5 ml NP4-100
NanoPOP-4 (3130/3130XL) 10 ml NP4-101
NanoPOP-4 (310/3100) 5 ml NP4-120
NanoPOP-4 (310/3100) 10 ml NP4-121
NanoPOP-6 (3130/3130XL) 5 ml NP6-100
NanoPOP-6 (3130/3130XL) 10 ml NP6-101
NanoPOP-6 (310/3100) 5 ml NP6-120
NanoPOP-6 (310/3100) 10 ml NP6-121
NanoPOP-7 (3130/3130XL) 5 ml NP7-100
NanoPOP-7 (3130/3130XL) 10 ml NP7-101
NanoPOP-7 (3730/3730XL) 28 ml NP7-300
NanoPOP-7 (3730/3730XL) 10 x 28 ml NP7-301
NanoPOP 10X running buffer 100 ml NPB-100
NanoPOP 10X running buffer 500 ml NPB-500
CE 10X running buffer 100 ml RBUF-100
CE 10X running buffer 500 ml RBUF-500
√ NanoPOP™ polymer for 3500 Genetic Analyzer coming soon, please inquire.
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sBrightDye® Terminator Cycle Sequencing Kit
√ No need for any change in protocol, settings or analysis parameters √ High Performance for sequencing of GT-rich regions √ Uniform peak hights and optimal signal balance √ Reduce your costs √ Robust design for high success rates
IntroductionThe BrightDye® Terminator Cycle Sequencing Kit is a reagent used for labeling the dideoxynucleoside triphosphates using the Sanger sequencing method. This facili-tates their identification when the products of DNA sequencing reac-tions are separated by gel or by capillary electrophoresis. Bright-Dye® Terminator Cycle Sequenc-ing Kit is compatible with v3.1 sequencing chemistry setting. Ideal for de novo sequencing, re-sequencing and finishing with PCR product, Plasmid, Cosmid and BAC templates.
Guaranteed PerformanceAll BrightDye® Terminator se-quencing reagents are tested twice for quality. First for correct formulation and secondly for con-sistent and reliable performance on sequencing systems.
The new solution for your DNA sequencing workflow
Applications - Ideal for PCR products and
de novo sequencing - Sequencing of difficult tem-
plates - Long-read sequencing - Sequencing accross all tem-
plate types (plasmid, PCR Products, BACs and cosmids)
- Excellent mixed-base detec-tion
- Sequencing short PCR products using rapid electro-phoresis run modules
Platforms310, 3100, 3130, 3130XL, 3500, 3500XL, 3730 & 3730XL Genetic Analyzers.
ContentAll kits include BrightDye Termi-nator ready reaction premix, 5X sequencing buffer, pGEM control and -21M13 primer.
Name Description P/N
BrightDye®Terminator Cycle Sequencing Kit 10 rxn, contains 80 µl 2.5X Ready Reaction Premix BDT3-50
BrightDye® Terminator Cycle Sequencing Kit 100 rxn, contains 800 µl 2.5X Ready Reaction Premix BDT3-100
BrightDye® Terminator Cycle Sequencing Kit 1000 rxn, contains 8 ML 2.5X Ready Reaction Premix BDT3-200
BrightDye® Terminator Cycle Sequencing Kit 5000 rxn, contains 40 ML 2.5X Ready Reaction Premix BDT3-300
BrightDye® Terminator Cycle Sequencing Kit 25000 rxn, contains 200 ML 2.5X Ready Reaction Premix BDT3-400
*Only available in the Benelux, for other countries please contact us.
FastGene® DyeTerminator Removal Kit
√ Manual sequencing product purification √ Cost efficient √ Long shelf life - storage at room temperature
High quality purification of DyeTerminator sequencing reactions by gel-filtration
IntroductionFastGene® DyeTerminator Re-moval Kit removes unincorperat-ed DyeTerminator molecules from sequencing samples using an ef-ficient and reasonably priced gel filtration. The Kit includes the gel filtration, resuspension buffer and filter spin columns. An Aliquot of the matrix is mixed with resus-pension buffer, then the equili-brated matrix is transferred to the filter spin column. After a brief spin down, up to 20 µl of the se-quencing reaction mix can be pu-rified on one column.
Name Description P/N
Nippon Fastgene®
Dye Terminator Removal kit50 preps FG-9411
SpecificationsThe maximum sample volume is 20 µl sequencing reaction. The ob-tained DNA recovery is over 90%.
The desired DNA is eluted by a fi-nal centrifugation step. All kits can be stored at room temperature.
This figure shows the long read lengths with BrightDye® Terminator on a 3730XL Genetic Analyzer. pGEM control DNA was used in combination with M13 primers. Sequences were run under filter set Z.
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sD-pure™ DyeTerminator Removal Kit
√ Proprietary formula √ No C-degradation √ Very Competitive prices
IntroductionD-pure™ DyeTerminator remov-al kit is a magnetic bead-based high-throughput purification method for DNA sequencing reac-tions. The kit consists of a mag-netic bead solution and an elu-tion buffer. Each component has been optimized for removing salts and unincorporated DyeTermina-tors from DNA sequencing reac-tion mixtures. The purified DNA products are more stable than the leading competitor’s magnetic beads. The system can be eas-ily adapted in your current liquid handling system. Please visit our website for the Hamilton applica-tion note, describing how to use D-pure in an automated liquid handling system.
High quality purification of DyeTerminator sequencing reactionsby magnetic beads
Name Description P/N
D-pure™ kit 5 ml (total volume)500 preps (96-well format)
DP-005
D-pure™ kit 50 ml (total volume)5000 preps (96-well format)
DP-050
D-pure™ kit 500 ml (total volume)50.000 preps (96-well format)
DP-500
High quality purification of DyeTerminator sequencing reactionsby magnetic beads
Fluorescent DNA Size Standards
√ Red DNA Size Standard (GeneScan™ 500 ROX® alternative)
√ Orange DNA Size Standard (GeneScan™ 500 LIZ® alternative) √ Double Peak DNA Size Standard for monitoring resolution performance
of the installed capillary arrays
IntroductionMCLAB’s DNA Size Standard prod-ucts are internal lane standards that are intended to be used in assigning sizes to DNA fragments on fluorescence-detecting instru-ments. The common applica-tions include genotyping and DNA fragment Analysis. Each of these standards consists of 15 DNA fragments, 50, 75, 100, 139, 150, 160, 200, 250, 300, 340, 350, 400, 450, 490 and 500 bp. The fragments are single-stranded and fluorescence-labeled either with carboxy-x-rhodamine (ROX® dye) or MCLAB’s proprietary fifth orange dye. Size fragments are evenly distributed and can be used for very accurate size calling. Compatible with ABI Genetic Ana-lyzers: 310, 3100, 3130/3130XL, 3500 and 3730/3730XL.
Assign sizes to DNA fragments
Red DNA Size StandardThis size standard has been adapted to DNA fragment analysis software, e.g. GeneMapper® and Peak Scanner® by using the same parameters as ABI’s GS 500 ROX® Size Standard.
Orange DNA Size StandardThe orange DNA size standard can be used with the same settings as for ABI’s GS 500 LIZ® size stand-ard.
Double Peak DNA Size StandardThis size standard is similar to the red DNA size standard, except every fragment is accompanied by an one-base-plus band. For ex-ample, 50 becomes 50 and 51, 75 becomes 75 and 76, etc. This sin-gle base separation is very useful for monitoring the performance of the installed capillary arrays.
Name Description P/N
Red DNA Size Standard 800 analysis (400µl) DSMR-100
Orange DNA Size Standard 800 analysis (400µl) DSMO-100
Double Peak DNA Size Standard 800 analysis (400µl) DSMD-100
310 CapillariesHigh reliability and low fluorescent background
Introduction310 Capillaries are bare fused silica capillary tubing’s, 47cm x 50μm (36 cm well-to-read) and 61cm x 50μm (50 cm well-to-read). They are designed and optimized for fragment analysis or sequencing applications on 310 Genetic Analyzer.
Name Description P/N
310 Capillary 47 cm, 5 pieces CAP-47
310 Capillary 61 cm, 2 pieces CAP-61
The capillaries have a low fluorescent background and are very reliable (>100 runs/capil-lary).
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AddressNimaGen BVLagelandseweg 566545 CG NijmegenThe Netherlands
Contact detailsPhone: +31 (0)24 820 02 41Fax: +31 (0)24 358 02 59E-mail: [email protected]: www.nimagen.com
International account managerJoop TheelenPhone: +31 (0)24 820 02 41E-mail: [email protected]
Holland & BelgiumLeticia van der MeerPhone: +31 (0)24 820 02 41E-mail: [email protected]
United Kingdom Zulfiqar MajeedPhone: +44 (0)121 468 00 15E-mail: [email protected]
GermanyRegionNiedersachsen, NRW, Rheinland-Pfalz, Hessen, Saarland, Baden-Württemberg, Bayern (München excluded) & Hamburg.
Melanie SchwarzPhone: +49 (0)211 9870 1237Email: [email protected]
RegionSchleswig-Holstein, Mecklenburg-Vorpommern, Brandenburg, Berlin, Sachsen-Anhalt, Sachsen, Thüringen, Bremen & München.
Tordis BorowskiPhone: +49 (0)157 3773 9676Email: [email protected]
www.nimagen.com
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.© 2012 NimaGen, part of Biolegio Nijmegen, The Netherlands. All rights reserved.Changes in configuration or specifications may be applied without prior notification.
“NanoPOP”, “NanoPOP-4”, “NanoPOP-6”, “NanoPOP-7” are trademarks of Molecular Cloning Lab, Inc. in the U.S. “BrightDye” is a registered trademark of ICloning, Inc. China. “FastGene” is a registered trademark of Nippon Genetics Europe GmbH.“D-pure” is a trademark of NimaGen, part of Biolegio Nijmegen, The Netherlands.“GeneScan”, “ROX”, “LIZ” are registrated trademarks and “Peak Scanner” is a trademark of Life Technologies, Carlsbad, USA.All other trademarks are the sole property of their respective owners.
