Fluorescence emission spectra of (a) DLE (5.00 M), (b) DLE/BSA(5.00 M /2.50 M ) and (c-D)DLE/BSA/Hg2+ with different concentrations of Hg2+ from 5 M to 30 M in 50mM NaAC solution. Inset shows fluorescence photographs of (a), (b) and (d) under illumination with 365 nm light.

Fig.1

d b a

Fluorescence emission spectra of (a) DLS (5.00 M), (b) DLS/BSA(5.00 M /2.50 M ) and (c-g)DLS/BSA/Hg2+ with different concentrations of Hg2+ from 5 M to 200 M in 50 mM NaAC solution. Inset shows fluorescence photographs of (a), (b) and (g) under illumination with 365 nm light.

g b a

Fig.2

Fluorescence emission spectra of (a) DLP (5.00 M), (b) DLP/BSA(5.00 M /2.50 M ) and (c-h)DLP/BSA/Hg2+ with different concentrations of Hg2+ from 5 M to 200 M in 50 mM NaAC solution. Inset shows fluorescence photographs of (a), (b) and (h) under illumination with 365 nm light.

abh

Fig.3

Fluorescence emission spectra of (a) DLT(5.00 M), (b) DLT/BSA (5.00 M /2.50 M ) and (c-j)DLT/BSA/Hg2+ with different concentrations of Hg2+ from 5 M to 200 M in 50 mM NaAC solution. Inset shows fluorescence photographs of (a), (b) and (j) under illumination with 365 nm light.

abj

Fig.4

(A) Fluorescence emission spectra of DLE(30 µM) upon addition of Hg2+ (from 5 to 150 µM) in 50.0 mM NaAC solution. The excitation length is 340 nm.

DLE + Hg2+ 5µM

(B) Estimation of binding constant of DLE (30 µM) with Hg2+ : the plot based on the 1:1 binding model :I0/(I-I0) versus [Hg2+]-1

Fig.5

150µM