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Flow Cytometry - Leinco Technologies · Flow Cytometry Reporter Molecule MW (g/mol) (x10-3) Abs. Max. (nm) (M-1 cm-1) Em. Max (nm) Allophycocyanin (APC) 104 650 700,000 660 Fluorescein

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Page 1: Flow Cytometry - Leinco Technologies · Flow Cytometry Reporter Molecule MW (g/mol) (x10-3) Abs. Max. (nm) (M-1 cm-1) Em. Max (nm) Allophycocyanin (APC) 104 650 700,000 660 Fluorescein

excellence in early discovery research™

Flow Cytometry

Page 2: Flow Cytometry - Leinco Technologies · Flow Cytometry Reporter Molecule MW (g/mol) (x10-3) Abs. Max. (nm) (M-1 cm-1) Em. Max (nm) Allophycocyanin (APC) 104 650 700,000 660 Fluorescein

Leinco Technologies has been a premier developer and manufacturer of innovative flow cytometry reagents since 1992. Our flow cytometry products have applications in a broad range of fields, including molecular biology, cell biology, pathology, immunology and cancer research. Antibodies that specifically bind to intracellular antigens or cell surface receptors can be optimally tagged with a variety of fluorescent dyes to allow for multicolor analysis of cellular subsets.

Leinco Technologies’ legendary Easy-Lyse™ Whole Blood Erythrocyte Lysing Kit has been designed to gently lyse erythrocytes while maintaining exquisite populations of leukocytes.

We offer a wide range of DyLight®, biotin-, FITC-, R-PE-, APC- and PerCP-conjugated monoclonal antibodies designed to immunophenotype cell populations.

Flow Cytometry

Reporter Molecule MW (g/mol) (x10-3) Abs. Max. (nm) (M-1 cm-1) Em. Max (nm)

Allophycocyanin (APC) 104 650 700,000 660

Fluorescein (FITC) 0.473 495 74,000 519

R-Phycoerythrin (R-PE) 240 565.5 1,960,000 578

Texas Red 0.817 583 116,000 603

PerCP 35 482 40,600 675

DyLight® 488 0.08 493 70,000 518

DyLight® 549 1.007 562 150,000 576

DyLight® 594 1.059 593 80,000 618

DyLight® 649 1.033 654 250,000 673

Note: Depending upon excitation and emission requirements, a variety of other reporter molecules are available to be custom conjugated to antibodies or proteins.

Commonly Used Fluorophores Available

When performance and results are critical, researchers turn to Leinco Technologies for their key research tools.

Page 3: Flow Cytometry - Leinco Technologies · Flow Cytometry Reporter Molecule MW (g/mol) (x10-3) Abs. Max. (nm) (M-1 cm-1) Em. Max (nm) Allophycocyanin (APC) 104 650 700,000 660 Fluorescein

PRODUCT # SURFACETAG™ HUMAN CD MARKERS CLONE

C109 Anti-Human CD2 G11

C105 Anti-Human CD3 UCHT-1

C121 Anti-Human CD4 RPA-T4

C113 Anti-Human CD5 UCHT-2

C117 Anti-Human CD8 UCHT-4

C137 Anti-Human CD10 (CALLA) SJ5-1B4

C171 Anti-Human CD11a (LFA-1α Subunit Integrin αMβ2) 38

C133 Anti-Human CD11b (Mac-1 Integrin αMβ2) 44

C145 Anti-Human CD11c (gp 150/95) 3.9

C149 Anti-Human CD13 (Myeloid Cells) 2245

C153 Anti-Human CD14 (Monocytes) UCHM-1

C157 Anti-Human CD15 (Granulocytes) TG1

C141 Anti-Human CD19 (Pan B-Cells) SJ25-C1

C1602 Anti-Human CD20 2H7

C179 Anti-Human CD22 RFB-4

C187 Anti-Human CD41 (gp llb/llla) M148

C183 Anti-Human CD44 (Pgp-1) E1/2

C129 Anti-Human CD45RO UCHL-1

C167 Anti-Human ICAM-1 (CD54) 15.2

C175 Anti-Human CD56 (NCAM) ERIC-1

C287 Anti-Human CD57/HNK-1 (Natural Killer Cells) VC1.1

C191 Anti-Human CD61 (Integrin β3) F11

C163 Anti-Human CD71 (Transferrin Receptor) T56/14

***For specific information on each reagent please visit www.Leinco.com***

PRODUCT # SURFACETAG™ MOUSE CD MARKERS CLONE

C207 Anti-Mouse CD3ε 500A2

C211 Anti-Mouse CD4 GK1.5

C215 Anti-Mouse CD8a (Ly 2) (All Alleles) 53-6.7

C297 Anti-Mouse CD8a (Ly 2.2) 2.43

C223 Anti-Mouse CD11a (LFA-1α) I21/7

C239 Anti-Mouse CD11a (LFA-1α) FD441.8

C227 Anti-Mouse CD11b (Mac-1 αM chain) M1/70

C231 Anti-Mouse CD18 (LFA-1, Integrin β2 Chain) C71/16

C235 Anti-Mouse CD24 (Heat Stable Antigen, Nectadrin) R7129.7

C243 Anti-Mouse CD28 37.58

C247 Anti-Mouse CD32/CD16 (Fcγ II/III Receptor) YT1.24

C255 Anti-Mouse CD45 (LCA) (Leukocyte Common Antigen) I3/2.3

C263 Anti-Mouse CD45RB 16A

C275 Anti-Mouse CD71 (Transferrin Receptor) R17217

C279 Anti-Mouse CD90 (Thy 1) (Activating) HK2.1

C271 Anti-Mouse CD90 (Thy 1, non-polymorphic) T24/31

C283 Anti-Mouse CD90 (Thy 1.2) 30-H12

D104 Anti-Mouse Dendritic Cells 33D1

G100 Anti-Mouse Granulocytes (Ly-6G, LY-6C, Gr-1, Myeloid) RB6-8C5

C267 Anti-Mouse ICAM-1 BE29G1

C1189 Anti-Mouse IL-2 Rα PC61

I-401 Anti-Mouse Interferon α/β Receptor (IFNAR1/CD118) MAR1-5A3

L126 Anti-Mouse Ly-6C (Activating) HK1.4

L131 Anti-Mouse Ly-6C (Non-Activating) 7B10

N106 Anti-Mouse Natural Killer Cells PK136

B100 Anti-Mouse Pre B-Cells BP-1

S102 Anti-Mouse Sca-1 (Ly-6A/E, Stem Cells) 38/42

T137 Anti-Mouse ThB 114.8.1

C251 Anti-Mouse/Human CD44 (Pgp-1, Ly-24, H-CAM) IM7

C259 Anti-Mouse/Human CD45R (B220) (Pan B-Cell) RA3-6B2

Flow Cytometry Reagents** The following is a non-inclusive list of our products. For a complete listing of all available reagents, please visit our website at Leinco.com or contact us today at (800) 538-1145.

Page 4: Flow Cytometry - Leinco Technologies · Flow Cytometry Reporter Molecule MW (g/mol) (x10-3) Abs. Max. (nm) (M-1 cm-1) Em. Max (nm) Allophycocyanin (APC) 104 650 700,000 660 Fluorescein

359 Consort DriveSt. Louis, Missouri 63011Phone: (800) 538-1145Fax: (636) 527-5545www.Leinco.com


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