Fisher SclentlRe - University of Nevada, RenoFisher SclentlRe Creation Date 25-Mar-2010 Material Safety Data Sheet Revision Date 25-Mar-2010 Revision Number 1 Product Name SurePrepTl

Fisher SclentlRe

Creation Date 25-Mar-2010

Material Safety Data SheetRevision Date 25-Mar-2010 Revision Number 1

Product Name SurePrepTl RNAlDNA/Protein Purification Kit, Lysis Solution

Cat No. BP2802-50

Synonyms No information available.

Recommended Use Laboratory chemicals

CompanyFisher ScientificOne Reagent LaneFair Lawn, NJ 07410Tel: (201) 796-7100

Emergency Telephone NumberCHEMTREC(ß, Inside the USA: 800-424-9300CHEMTREC(ß, Outside the USA: 703-527-3887

DANGER!Emergency Overview

Causes burns by all exposure routes. Harmful by inhalation, in contact with skin and if swallowed. Contact with acidsliberates very toxic gas. Harmful to aquatic organisms, may cause long-term adverse effects in the aquatic environment.

Appearance No information available. Physical State Liquid odor No information available

Target Organs Respiratory system, Eyes, Skin, Gastrointestinal tract (GI)

Potential Health Effects

Acute EffectsPrinciple Routes of Exposure

EyesSkinInhalationIngestion

Causes bums.Causes bums. Harmful in contact with skin.Causes bums. Harmful by inhalation.Harmful if swallowed. Causes bums. Ingestion may cause gastrointestinal irritation, nausea,vomiting and diarrhea.

Chronic Effects None known.

See Section 11 for additional Toxicological information.

Aggravated Medical Conditions No information available.

Page 1 I 8

Thermo Fisher Scientific - SurePrepTM RNAlDNAlProteinPurification Kit, Lysis Solution

Revision Date 25-Mar-2010

HazlNon-hazComponent CAS-No Weiaht %

Thiocvanic acid, comoound with auanidine (1: 1) 593-84-0 45 - 50Nuclease Free Water 7732-18-5 35 - 38

Citrate, sodium, dihvdrate 6132-04-3 13-15

Eye Contact Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes.Immediate medical attention is required.

Skin Contact Wash off immediately with plenty of water for at least 15 minutes. Immediate medical attentionis required.

Inhalation Move to fresh air. If breathing is diffcult, give oxygen. Do not use mouth-to-mouth resuscitationif victim ingested or inhaled the substance; induce artificial respiration with a respiratorymedical device. Immediate medical attention is required.

Ingestion Do not induce vomiting. Call a physician or Poison Control Center immediately.

Notes to Physician Treat symptomatically.

Flash PointMethod

Not applicableNo information available.

Autoignition TemperatureExplosion Limits

UpperLower

No information available.

No data availableNo data available

Suitable Extinguishing Media Substance is nonflammable; use agent most appropriate toextinguish surrounding fire..

Unsuitable Extinguishing Media No information available.

Hazardous Combustion Products No information available.

Sensitivity to mechanical impactSensitivity to static discharge

No information available.No information available.

Specific Hazards Arising from the ChemicalThermal decomposition can lead to release of irritating gases and vapors.

Protective Equipment and Precautions for FirefightersAs in any fire, wear self-contained breathing apparatus pressure-demand, MSHAINIOSH (approved or equivalent) and full protectivegear

NFPA Health 3 Flammabilty 0 Instabilty 1 Physical hazards N/A

Page 2 I 8


Revision Date 25-Mar-201 0

Personal Precautions Use personal protective equipment. Ensure adequate ventilation. Do not get in eyes, on skin,or on clothing.

Should not be released into the environment.Environmental Precautions

Methods for Containment and Clean Soak up with inert absorbent materiaL. Keep in suitable and closed containers for disposaL.Up

Handling Use only under a chemical fume hood. Wear personal protective equipment. Do not breathevapors or spray mist. Do not get in eyes, on skin, or on clothing. Keep away from acids.

Storage Keep containers tightly closed in a dry, cool and well-ventilated place. Corrosives area.

Engineering Measures Use only under a chemical fume hood. Ensure that eyewash stations and safety showers areclose to the workstation location.

Exposure Guidelines This product does not contain any hazardous materials with occupational exposure limitsestablished by the region specific regulatory bodies.

N/OSH /DLH: Immediately Dangerous to Life or Health

Personal Protective EquipmentEye/face Protection

Skin and body protectionRespiratory Protection

Wear appropriate protective eyeglasses or chemical safety goggles as described by OSHA'seye and face protection regulations in 29 CFR 1910.133 or European Standard EN166Wear appropriate protective gloves and clothing to prevent skin exposureFollow the OSHA respirator regulations found in 29 CFR 1910.134 or European Standard EN149. Use a NIOSH/MSHA or European Standard EN 149 approved respirator if exposure limitsare exceeded or if irritation or other symptoms are experienced

~i91: "'Y.CAlJoi~CÌlM ': .

Physical StateAppearanceodorOdor ThresholdpHVapor PressureVapor DensityViscosityBoilng Point/Range

Melting Point/RangeDecomposition temperature °C

Flash PointEvaporation Rate

SpeCific GravitySolubilty

LiquidNo information available.No information availableNo information available.6No information available.No information available.No information available.No information available.No information available.No information available.Not applicable

No information available.No information available.No information available.

Page 3 I 8



log Pow No data available

Stabilty Stable under normal conditions. Light sensitive.

Incompatible Materials

Incompatible products. Excess heat. Exposure to light.

Acids, Strong oxidizing agents

Conditions to Avoid

Hazardous Decomposition Products Carbon monoxide (CO), Carbon dioxide (C02), Nitrogen oxides(NOx), Sulfur oxides, Hydrogen cyanide (hydrocyanic acid)

Hazardous Polymerization Hazardous polymerization does not occur

Hazardous Reactions. Contact with acids liberates very toxic gas..

Acute Toxicity

Product Information No acute toxicity information is available for this product

Component Information

Irritation Causes burns by all exposure routes

Toxicologically SynergisticProducts


Chronic Toxicity

Carcinogenicity There are no known carcinogenic chemicals in this product

Sensitization No information available.

Mutagenic Effects

Reproductive Effects

Developmental Effects




Teratogenicity No information available.

Other Adverse Effects The toxicological properties have not been fully investigated.. See actual entry in RTECS forcomplete information.

Endocrine Disruptor Information No information available

Page 4 I 8



EcotoxicityHarmful to aquatic organisms, may cause long-term adverse effects in the aquatic environment.

Persistence and Degradabilty

Bioaccumulation/ Accumulation

Mobilty

No information available



Waste Disposal Methods Chemical waste generators must determine whether a discarded chemical is classified as ahazardous waste. Chemical waste generators must also consult local, regional, and nationalhazardous waste regulations to ensure complete and accurate classification

DOT

UN-NoProper Shipping NameProper technical nameHazard ClassPacking Group

TDG

UN-NoProper Shipping NameHazard Class

Packing Group

lATA


Packing Group

IMDGIIMO


Packing Group

UN1760CORROSIVE LIQUIDS, N.O.S.(GUANIDINE THIOCYANATE)8III

UN1760CORROSIVE LIQUIDS, N.O.S.8III

UN1760CORROSIVE LIQUIDS, N.D.S.8III

UN1760CORROSIVE LIQUIDS, N.D.S.8III

Page 5 I 8

Thermo Fisher Scientifc - SurePrepTM RNAlDNAlProteinPurification Kit, Lysis Solution


IntemationallnventoriesComponent TSCA DSL NDSL EINECS ELINCS NLP PICCS ENCS AICS CHINA KECL

Thiocyanic acid, compound X X - 209-812- - X - X X -

with auanidine (1: 1) 1

Nuclease Free Water X X - - - X - X XX

Citrate, sodium, dihydrate - - - - - X - X X -

Legend:X - ListedE - Indicates a substance that is the subject of a Section 5(e) Consent order under TSCA.F - Indicates a substance that is the subject of a Section 5(t) Rule under TSCA.N - Indicates a polymeric substance containing no free-radical initiator in it inventory name but is considered to cover the designatedpolymer made with any free-radical initiator regardless of the amount used.P - Indicates a commenced PMN substanceR - Indicates a substance that is the subject of a Section 6 risk management rule under TSCA.S - Indicates a substance that is identifed in a proposed or final Signifcant New Use RuleT - Indicates a substance that is the subject of a Section 4 test rule under TSCA.XU - Indicates a substance exempt from reporting under the Inventory Update Rule, i.e. Partial Updating of the TSCA Inventory Data BaseProduction and Site Report (40 CFR 710(B).

Y1 - Indicates an exempt polymer that has a number-average molecular weight of 1,000 or greater.Y2 - Indicates an exempt polymer that is a polyester and is made only frm reactants included in a specified list of low concern reactants thatcomprises one of the eligibilit crieria for the exemption rule.

u.s. Federal Regulations

TSCA 12(b) Not applicable

SARA 313Not applicable

SARA 311/312 Hazardous CategorizationAcute Health HazardChronic Health HazardFire HazardSudden Release of Pressure HazardReactive Hazard

YesNoNoNoNo

Clean Water ActNot applicable

Clean Air ActNot applicable

OSHANot applicable

Page 6 I 8



CERCLANot Applicable

California Proposition 65This product does not contain any Proposition 65 chemicals.

State Right-to-KnowNot applicable

u.S. Department of TransportationReportable Quantity (RQ): NDOT Marine Pollutant NDOT Severe Marine Pollutant N

u.S. Department of Homeland SecurityThis product does not contain any DHS chemicals.

Other International Regulations

Mexico - Grade No information available

Canada

This product has been classified in accordance with the hazard criteria of the Controlled Products Regulations (CPR) and theMSDS contains all the information required by the CPR.

WHMIS Hazard ClassD1 B Toxic materials

E Corrosive material

Prepared By Regulatory AffairsThermo Fisher ScientificTel: (412) 490-8929


Print Date 25-Mar -2010

Revision Summary "***", and red text indicates revision

Page 7 I 8



DisclaimerThe information provided on this Safety Data Sheet is correct to the best of our knowledge, information and belief at the dateof its publication. The information given is designed only as a guide for safe handling, use, processing, storage,transporttion, disposal and release and is not to be considered as a warrnty or quality specification. The information

relates only to the specific material designated and may not be valid for such material used in combination with any othermaterial or in any process, unless specified in the text

End of MSDS

Page 8 I 8

Fisher SclentlRe


Material Safety Data SheetRevision Date 25-Mar-201 0 Revision Number 1

Product Name SurePrepTl RNA/DNA/Protein Purification Kit, RNA Wash Solution

Cat No. BP2802-50




Emergency Telephone NumberCHEMTREC(ß, Inside the USA: 800-424-9300CHEMTREC(ß, Outside the USA: 703-527 -3887

CAUTION!Emergency Overview

May cause eye, skin, and respiratory tract irritation. The toxicological properties have not been fully investigated.

IIppearance Clear Colorless Physical State Liquid odor No information available

Target Organs


Liver, Kidney, Blood



May cause irritation.May cause irritation. May be harmful in contact with skin.May cause irritation of respiratory tract. May be harmful if inhaled.May be harmful if swallowed. Ingestion may cause gastrointestinal irritation, nausea, vomitingand diarrea.

Chronic Effects Experiments have shown reproductive toxicity effects on laboratory animals. May causeadverse liver effects. May cause adverse kidney effects.



Page 1 I 8

Thermo Fisher Scientific - SurePrepTM RNAlDNAlProteinPurification Kit, RNA Wash Solution



Nuclease Free Water 7732-18-5 98 - 99Tris (hvdroxvmethvl\ aminomethane 77 -86-1 1.0 - 2.0

Ethylenediamine tetra acetic acid (EDT A) 60-00-4 -: 1.0

Eye Contact Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtainmedical attention.

Inhalation

Wash off immediately with plenty of water for at least 15 minutes. Get medical attentionimmediately if symptoms occur.

Move to fresh air. If breathing is diffcult, give oxygen. Get medical attention immediately ifsymptoms occur.

Skin Contact

Ingestion Do not induce vomiting. Obtain medical attention.


Flash PointMethod

Not applicable



UpperLower




Unsuitable Extinguishing Media

Hazardous Combustion Products








Page 2 I 8

Thermo Fisher Scientific - SurePrepTM RNAlDNAlProteinPurifcation Kit, RNA Wash Solution


Personal Precautions Use personal protective equipment. Ensure adequate ventilation. Avoid contact with skin, eyesand clothing.

Environmental Precautions Should not be released into the environment.

Methods for Containment and Clean Soak up with inert absorbnt materiaL. Keep in suitable and closed containers for disposaL.Up

Handling Wear personal protective equipment. Ensure adequate ventilation. Do not breathe vapors orspray mist. Avoid contact with skin, eyes and clothing.

Storage Keep containers tightly closed in a dry, cool and well-ventilated place.

Engineering Measures Ensure adequate ventilation, especially in confined areas. Ensure that eyewash stations andsafety showers are close to the workstation location.





Wear appropriate protective eyeglasses or chemical safety goggles as described by OSHA'seye and face protection reulations in 29 CFR 1910.133 or European Standard EN166Wear appropriate protective gloves and clothing to prevent skin exposureFollow the OSHA respirator regulations found in 29 CFR 1910.134 or European Standard EN149. Use a NIOSH/MSHA or European Standard EN 149 approved respirator if exposure limitsare exceeded or if irritation or other symptoms are experienced

Physical StateAppearanceodorOdor ThresholdpHVapor PressureVapor DensityViscosityBoiling Point/RangeMelting Point/RangeDecomposition temperature °C


Specific Gravity

Solubilty

LiquidClear ColorlessNo information availableNo information available.7.5No information available.No information available.No information available.No information available.No information available.No information available.Not applicableNo information available.No information available.No information available.

Page 3 I 8



No data availablelog Pow

Stabilty Stable under normal conditions.

Conditions to Avoid Excess heat.

Incompatible Materials None known

Hazardous Decomposition Products Thennal decomposition can lead to release of irrtating gases andvapors


Hazardous Reactions. None under normal processing.

Acute Toxicity


LD50 DerralNot listedNot listed

LC50 InhalationNot listedNot listed

Irritation No information available.



Chronic Toxicity



Mutagenic Effects Mutagenic effects have occurred in experimental animals.

Reproductive Effects Experiments have shown reproductive toxicity effects on laboratory animals.

Developmental Effects Developmental effects have occurred in experimental animals.

Teratogenicity Teratogenic effects have occurred in experimental animals..

Page 4 I 8

Thermo Fisher Scientifc - SurePrepTM RNAlDNAlProteinPurification Kit, RNA Wash Solution




Ecotoxicity

Freshwater FishNot listed

MicrotoxNot listed

Water FleaEC50 96 h ;.100 mg/L

Persistence and Degradability No information available

Bioaccumulation/ Accumulation No information available

Mobilty No information available


DOT Not regulated

TDG Not regulated

lATA Not regulated

IMDGIIMO Not regulated

IntemationallnventoriesComponent I TSCA i DSL I NDSL I EINECS I ELINCS I NLP I PICCS I ENCS I AICS I CHINA i KECL I

Page 5 I 8



Legend:X - ListedE - Indicates a substance that is the subject of a Section 5(e) Consent order under TSCA.F - Indicates a substance that is the subject of a Section 5(f) Rule under TSCA.N - Indicates a polymeric substance containing no free-radical initiator in it inventory name but is considered to cover the designatedpolymer made with any free-radical initiator regardless of the amount used.P - Indicates a commenced PMN substanceR - Indicates a substance that is the subject of a Section 6 risk management rule under TSCA.S -Indicates a substance that is identifed in a proposed or final Signifcant New Use RuleT - Indicates a substance that is the subject of a Section 4 test rule under TSCA.XU - Indicates a substance exempt from reporting under the Inventory Update Rule, i.e. Partial Updating of the TSCA Inventory Data BaseProduction and Site Report (40 CFR 710(B).

Y1 - Indicates an exempt polymer that has a number-average molecular weight of 1,000 or greater.Y2 - Indicates an exempt polymer that is a polyester and is made only from reactants included in a specifed list of low concern reactants thatcomprises one of the eligibilit crieria for the exemption rule.

U.S. Federal Regulations




NoNoNoNoNo

Clean Water Act

Component CWA - Hazrdous CWA - Reportble CWA - Toxic Pollutants CWA - Priori PollutantsSubstances Quantities

Ethylenediamine tetraacetic acid X 5000 Ib - -

(EOTA)


OSHANot applicable

CERCLAThis material, as supplied, contains one or more substances regulated as a hazardous substance under the ComprehensiveEnvironmental Response Compensation and Liability Act (CERCLA) (40 CFR 302)

Page 6 I 8



Hazardous Substances RQs

5000 Ib

CERCLA EHS RQs


State Right-to-Know

MassachusettX

New JerseX

Ilinois Rhode Island

u.s. Department of TransportationReportable Quantity (RQ): YDOT Marine Pollutant NDOT Severe Marine Pollutant N

u.s. Department of Homeland SecurityThis product does not contain any DHS chemicals.



Canada


WHMIS Hazard ClassNon-controlled


25-Mar-2010Creation Date

Print Date 25-Mar -201 0

Revision Summary ".**", and red text indicates revision

Page 7 I 8





End of MSDS

Page 8 I 8

Fisher Sclentille



Product Name SurePrepTl RNAlDNAlProtein Purification Kit, Elution Solution

Cat No. BP2802-50

Synonyms Nuclease Free Water




CAUTION!Emergency Overview

Non-hazardous substance. Handle in accordance with good industrial hygiene and safety practice.

toppearance Clear Colorless Physical State Liquid odor odorless

Target Organs


None known.



Non-irritating during normal use.Non-irritating during normal use.Not an expected route of exposure.Low hazard for usual industrial or commercial handling.




Page 1 I 7

Thermo Fisher Scientific - SurePrepTM RNAlDNAlProteinPurification Kit, Elution Solution


HazlNon-hazCom onent

Nuclease Free WaterCAS-No

7732-18-5Wei ht %

100

Eye Contact Flush eyes with water as a precaution.

Skin Contact No hazards which require special first aid measures.

Inhalation No hazards which require special first aid measures.



Flash PointMethod



UpperLower







Specific Hazards Arising from the ChemicalNone known.




Personal Precautions Use personal protective equipment.

Environmental Precautions No special environmental precautions required.

Page 2 I 7




Handling Handle in accrdance with good industrial hygiene and safety practice. Wear personalprotective equipment.


Engineering Measures Ensure that eyewash stations and safety showers are close to the workstation location.

This product does not contain any hazardous materials with occupational exposure limitsestablished by the region specific regulatory bodies.

Exposure Guidelines




Wear appropriate protective eyeglasses or chemical safety goggles as described by OSHA'seye and face protection regulations in 29 CFR 1910.133 or European Standard EN 166Wear appropriate protective gloves and clothing to prevent skin exposureFollow the OSHA respirator regulations found in 29 CFR 1910.134 or European Standard EN149. Use a NIOSH/MSHA or European Standard EN 149 approved respirator if exposure limitsare exceeded or if irritation or other symptoms are experienced

Physical State

AppearanceodorOdor ThresholdpHVapor Pressure

Vapor DensityViscosityBoiling Point/RangeMelting Point/RangeDecomposition temperature °CFlash PointEvaporation Rate

Specific GravitySolubiltylog PowMolecular WeightMolecular Formula

LiquidClear ColorlessodorlessNo information available.7No information available.No information available.No information available.100°C 1212°FO°C 132°F

No information available.Not applicable

No information available.1.000No information available.No data available18.02H20

Stability Stable under normal conditions.

Page 3 I 7

Thermo Fisher Scientific - SurePrepTM RNAlDNAlProtein Revision Date 25-Mar-201 0Purification Kit, Elution Solution

Conditions to Avoid None known.Incompatible Materials None knownHazardous Decomposition Products None knownHazardous Polymerization Hazardous polymerization does not occurHazardous Reactions. None under normal processing.

Acute Toxicity


Component Information


Toxicologically Synergistic

ProductsNo information available.

Chronic Toxicity



Mutagenic Effects No information available.

Reproductive Effects No information available.

Developmental Effects No information available.


Other Adverse Effects The toxicological properties have not been fully investigated.


EcotoxicityContains no substances known to be hazardous to the environment or that are not degradable in waste water treatment plants.

Persistence and Degradabilty No information available


Page 4 I 7





DOT Not regulated

TDG Not regulated

lATA Not regulated


IntemationallnventoriesComponent TSCA DSL NDSL EINECs ELINCS NLP PICCS ENCS AICS CHINA KECL


Legend:X - ListedE -Indicates a substance that is the subject of a Section 5(e) Consent order under TSCA.F - Indicates a substance that is the subject of a Section 5(t) Rule under TSCA.N - Indicates a polymeric substance containing no free-radical initiator in it inventory name but is considered to cover the designatedpolymer made with any free-radical initiator reardless of the amount used.P - Indicates a commenced PMN substanceR - Indicates a substance that is the subject of a Section 6 risk management rule under TSCA.S - Indicates a substance that is identifed in a proposed or final Signifcant New Use RuleT - Indicates a substance that is the subject of a Section 4 test rule under TSCA.XU - Indicates a substance exempt from reporting under the Inventory Update Rule, i.e. Partial Updating of the TSCA Inventory Data BaseProduction and Site Report (40 CFR 710(B).


U.s. Federal Regulations

Page 5 I 7

Thermo Fisher Scientifc - SurePrepTM RNAlDNAlProteinPurification Kit, Elution Solution





NoNoNoNoNo



OSHANot applicable




u.s. Department of TransportationReportable Quantity (RQ): NDOT Marine Pollutant NDOT Severe Marine Pollutant N




Canada



Page 6 I 7





Print Date 25-Mar-2010




End of MSDS

Page 7 I 7

Fisher lelentlRe



Product Name SurePrepTl RNAIDNA/Protein Purification Kit, gDNA Wash Solution

Cat No. BP2802-50





~AUTION!Emergency Overview


Appearance Clear Colorless Physical State Liquid odor No information available

Target Organs





May cause irritation.May cause irritation. May be harmful in contact with skin.May cause irritation of respiratory tract. May be harmful if inhaled.May be harmful if swallowed. Ingestion may cause gastrointestinal irritation, nausea, vomitingand diarrhea.




Page 1/ 8

Thermo Fisher Scientific - SurePrepTM RNAlDNAlProteinPurification Kit, gDNA Wash Solution



Nuclease Free Water 7732-18-5 98 - 99Tris (hvdroxvmethvl\ aminomethane 77-86-1 1.0 - 2.0

Ethvlenediamine tetra acetic acid (EDTA) 60-00-4 .: 1.0


Inhalation

Wash off immediately with plenty of water for at least 15 minutes. Get medical attentionimmediately if symptoms occr.


Skin Contact



Flash PointMethod

Not applicable



UpperLower


No data available

No data available


Unsuitable Extnguishing Media No information available.







Page 2 1 8














Physical StateAppearanceodorOdor ThresholdpHVapor PressureVapor DensityViscosityBoiling Point/RangeMelting Point/RangeDecomposition temperature °C


Specific GravitySolubilty

LiquidClear ColorlessNo information available

No information available.9No information available.No information available.No information available.No information available.No information available.No information available.Not applicable


Page 3 1 8

Thermo Fisher Scientific - SurePrepTM RNAlDNAlProteinPurifcation Kit, gDNA Wash Solution




Conditions to Avoid Exæss heat.


Hazardous Decomposition Products Thermal decomposition can lead to release of irrtating gases andvapors



Acute Toxicity


LD50 DermlNot listedNot listed

LC50 InhalationNot listedNot listed




Chronic Toxicity



Mutagenic Effects



Mutagenic effects have occurred in experimental animals.

Experiments have shown reproductive toxicity effects on laboratory animals.

Developmental effects have occurred in experimental animals.

Teratogenicity Teratogenic effects have occurred in experimental animals..

Page 4 1 8

Thermo Fisher Scientific - SurePrepTM RNAlDNAlProteinPurification Kit, gONA Wash Solution




Ecotoxicity


MicrotoxNot listed

Water FleaEC50 96 h ;.100 mg/L





DOT Not regulated

TDG Not regulated

lATA Not regulated


IntemationallnventoriesComponent I TSCA I DSL I NDSL I EINECS I ELiNCS I NLP I PICCS I ENCS I AICS i CHINA I KECL I

Page 5 1 8



Legend:X - ListedE - Indicates a substance that is the subject of a Section 5(e) Consent order under TSCA.F - Indicates a substance that is the subject of a Section 5(f) Rule under TSCA.N - Indicates a polymeric substance containing no free-radical initiator in it inventory name but is considered to cover the designatedpolymer made with any free-radical initiator regardless of the amount used.P - Indicates a commenced PMN substanceR - Indicates a substance that is the subject of a Section 6 nsk management rule under TSCA.S - Indicates a substance that is identifed in a proposed or final Signifcant New Use RuleT - Indicates a substance that is the subject of a Section 4 test rule under TSCA.XU -Indicates a substance exempt from reporting under the Inventory Update Rule, i.e. Partial Updating of the TSCA Inventory Data BaseProduction and Site Report (40 CFR 710(B).

Y1 - Indicates an exempt polymer that has a number-average molecular weight of 1,000 or greater.Y2 - Indicates an exempt polymer that is a polyester and is made only from reactants included in a specifed list of low concern reactants thatcompnses one of the eligibilit criena for the exemption rule.




SARA 311/312 Hazardous CategorizationAcute Health HazardChronic Health Hazard

Fire HazardSudden Release of Pressure HazardReactive Hazard

NoNoNoNoNo

Clean Water Act

Component CWA - Hazrdous CWA - Reportble CWA - Toxic Pollutants CWA - Pnori PollutantsSubstances Quantities

Ethylenediamine tetraacetic acid X 5000 Ib - -

lEOTA)


OSHANot applicable


Page 6 1 8

Thermo Fisher Scientific - SurePrepTM RNAlONAlProteinPurification Kit, gONA Wash Solution


Hazrdous Substances RQs5000 Ib

CERCLA EHS RQs


State Right-to-Know

Massach usett

X

New Jer5eX

Ilinois Rhode Island

U.S. Department of Transporttion

Reportable Quantity (RQ): YDOT Marine Pollutant NDOT Severe Marine Pollutant N

U.S. Department of Homeland SecurityThis product does not contain any DHS chemicals.



Canada







Page 7 I 8

Thermo Fisher Scientific - SurePrepTM RNAlDNAlProteinPurification Kit, gONA Wash Solution


DisclaimerThe information provided on this Safety Data Sheet is correctto the best of our knowledge, information and belief at the dateof its publication. The information given is designed only as a guide for safe handling, use, processing, storage,transporttion, disposal and release and is not to be considered as a warrnty or quality specification. The information


End of MSDS

Page 8 1 8

Fisher SclentlRe



Product Name SurePrepTl RNA/DNA/Protein Purification Kit, gDNA Elution Buffer

Cat No. BP2802-50





ICAUTION!Emergency Overview


iAppearance Clear Colorless Physical State Liquid odor No information available

Target Organs









Page 1 I 7

Thermo Fisher Scientific - SurePrepTM RNAlDNAlProteinPurification Kit, gDNA Elution Buffer


HazlNon-hazComDonent CAS-No Weiaht 0/

Nuclease Free Water 7732-18-5 98 - 99Tris (hvdroxvmethvl) aminomethane 77 -86-1 1.0 - 2.0


Inhalation



Do not induce vomiting. Obtain medical attention.

Skin Contact

Ingestion


Flash PointMethod



UpperLower



Suitable Extnguishing Media Substance is nonflammable; use agent most appropriate toextinguish surrounding fire..






Protective Equipment and Precautions for FirefightersAs in any fire, wear self-contained breathing apparatus pressure-demand, MSHAlNIOSH (approved or equivalent) and full protectivegear


Page 2 17






Handling Wear personal protective equipment. Ensure adequate ventilation. Do not breathe vapors orspray mist Avoid contact with skin, eyes and clothing.



This product does not contain any hazardous materials with occupational exposure limitsestablished by the region specifiC regulatory bodies.

Exposure Guidelines





Physical State


Vapor DensityViscosityBoilng Point/Range

Melting Point/RangeDecomposition temperature °CFlash PointEvaporation Rate


LiquidClear ColorlessNo information availableNo information available.9No information available.No information available.No information available.No information available.No information available.No information available.Not applicable


Page 3 1 7










Acute Toxicity


LD50 DermalNot listed

LC50 InhalationNot listed




Chronic Toxicity



Mutagenic Effects






Teratogenicity

Other Adverse Effects


The toxicological properties have not been fully investigated.. See actual entry in RTECS forcomplete information.

Page 4 1 7




EcotoxicityDo not empty into drains.





DOT Not regulated

TDG Not regulated

lATA Not regulated


International InventoriesComponent TSCA DSL NDSL EINECS ELINCS NLP PICCS ENCS AICS CHINA KECL


Tris (hydroxymethyl) X X - 201-0- - X X X X KE-

aminomethane 4 01403X

Legend:

Page 5 1 7



x - ListedE - Indicates a substance that is the subject of a Section 5(e) Consent order under TSCA.F - Indicates a substance that is the subject of a Section 5(t) Rule under TSCA.N - Indicates a polymeric substance containing no fr-radical initiator in it inventory name but is considered to cover the designatedpolymer made with any fr-radical initiator regardless of the amount used.P - Indicates a commenced PMN substanceR - Indicates a substance that is the subject of a Section 6 risk management rule under TSCA.S - Indicates a substance that is identifed in a proposed or final Signifcant New Use RuleT - Indicates a substance that is the subject of a Section 4 test rule under TSCA.XU - Indicate a substance exempt from reporting under the Inventory Update Rule, i.e. Partial Updating of the TSCA Inventory Data BaseProduction and Site Report (40 CFR 710(B).

Y1 - Indicates an exempt polymer that has a number-average molecular weight of 1,000 or greater.Y2 - Indicates an exempt polymer that is a polyester and is made only from reactants included in a specified list of low concern reactants thatcomprises one of the eligibilit crieria for the exemption rule.

U.S. Federal Regulations




NoNoNoNoNo



OSHANot applicable




U.S. Department of Transporttion

Reportable Quantity (RQ): NDOT Marine Pollutant NDOT Severe Marine Pollutant N



Page 6 1 7




Canada






Revision Summary "..." , and red text indicates revision


relates only to the specific material designated and may not be valid for such material used in combination with any othermaterial or in any process, unless specifed in the text

End of MSDS

Page 7 1 7

Fisher lelentlRe



Product Name SurePrepTl RNA/DNA/Protein Purification Kit, Protein ColumnRegeneration Buffer

Cat No. BP2802-50





~AUTION!Emergency Overview


Appearance Clear Colorless Physical State Liquid odor No information available

Target Organs Liver, Kidney, Blood





Experiments have shown reproductive toxicity effects on laboratory animals. May causeadverse liver effects. May cause adverse kidney effects.


Chronic Effects


Page 1 1 7

Thermo Fisher Scientific - SurePrepTM RNAlDNAlProteinPurification Kit, Protein Column Regeneration Buffer


HazlNon-hazComDonent CAS-No Weiaht %

Nuclease Free Water 7732-18-5 98-99Tris (hydroxvmethvl\ aminomethane 77-86-1 1.0 - 2.0


Inhalation



Skin Contact



Flash PointMethod

Not applicable



UpperLower




Unsuitable Extnguishing Media No information available.






NFPA Health 1 Flammabilty 0 Instability 0 Physical hazards N/A

Page 2 1 7














Physical State

AppearanceodorOdor ThresholdpHVapor PressureVapor DensityViscosityBoilng Point/Range




LiquidClear ColorlessNo information availableNo information available.11

No information available.No information available.No information available.No information available.No information available.No information available.Not applicable


Page 3 1 7










Acute Toxicity


LD50 DermlNot listed

LC50 InhalationNot listed




Chronic Toxicity



Mutagenic Effects No information available.

Reproductive Effects Experiments have shown reproductive toxicity effects on laboratory animals.


Teratogenicity





Page 4 1 7






Bioaccumulationl Accumulation No information available



DOT Not regulated

TDG Not regulated

lATA Not regulated




Tris (hydroxymethyl) X X - 201-064- - X X X X KE-

aminomethane 4 01403X

Legend:

Page 5 1 7



x - ListedE -Indicates a substance that is the subject of a Section 5(e) Consent order under TSCA.F - Indicates a substance that is the subject of a Section 5(f) Rule under TSCA.N - Indicats a polymeric substance containing no free-radical initiator in it inventory name but is considered to cover the designatedpolymer made with any free-radical initiator reardless of the amount used.P -Indicates a commenced PMN substanceR - Indicates a substance that is the subject of a Section 6 risk management rule under TSCA.S -Indicates a substance that is identifed in a proposed or final Signifcant New Use RuleT - Indicates a substance that is the subject of a Section 4 tet rule under TSCA.XU - Indicates a substance exempt from reporting under the Inventory Update Rule, i.e. Partial Updating of the TSCA Inventory Data BaseProduction and Site Report (40 CFR 710(B).

Y1 - Indicates an exempt polymer that has a number-average molecular weight of 1,000 or greater.Y2 - Indicates an exempt polymer that is a polyester and is made only from reactants included in a specified list of low concern reactants thatcomprises one of the eligibilit crieria for the exemption rule.

U.s. Federal Regulations




NoNoNoNoNo



OSHANot applicable




U.S. Department of TransportationReportable Quantity (RQ): NDOT Marine Pollutant NDOT Severe Marine Pollutant N



Page 6 1 7




Canada









End of MsDS

Page 7/7

(l Fisher ScienifcMaterial Safety Data Sheet

Revision Date 25-Mar-201 0 Revision Number 1Creation Date 25-Mar-2010

Product Name SurePrepTl RNA/DNAlProtein Purification Kit, Column Activation andWash Buffer

Cat No. BP2802-50



CompanyFisher ScientificOne Reagent Lane

Fair Lawn, NJ 07410Tel: (201) 796-7100


!CAUTION!Emergency Overview


IAppearance Clear Colorless Physical State Liquid odor No information available

Target Organs


None known.







Page 1/ 8

Thermo Fisher Scientific - SurePrepTM RNAlDNAlProteinPurification Kit, Column Activation and Wash Buffer


HazlNon-hazComDonent CAS-No Weiaht %

Nuclease Free Water 7732-18-5 ;: 99Phosohoric acid 7664-38-2 oe 0.5

Citric acid 77 -92-9 oe 0.5

Sodium hydroxide 1310-73-2 oe 0.1


Skin Contact Wash off immediately with plenty of water for at least 15 minutes. Get medical attentionimmediately if symptoms occur.

Inhalation Move to fresh air. If breathing is diffcult, give oxygen. Get medical attention immediately ifsymptoms occur.



Flash PointMethod

Not applicable



UpperLower





Hazardous Combustion Products








Page 2 1 8









Exposure Guidelines

Comoonent ACGIH TLV OSHA PEL NIOSH IDLH

Phosphoric acid TWA: 1 mg/m3 (Vacated) TWA: 1 mg/m3 IDLH: 1000 mg/m3STEL: 3 mg/m3 (Vacated) STEL: 3 mg/m3 TWA: 1 mg/m3

TWA: 1 ma/m3 STEL: 3 mçi/m3

Sodium hydroxide Ceiling: 2 mg/m3 (Vacated) Ceiling: 2 mg/m3 IDLH: 10 mg/m3TWA: 2 ma/m3 Ceilino: 2 ma/m3

Component Quebec Mexico OEL (TWA) Ontario TWAEV

Phosphoric acid TWA: 1 mg/m3 TWA: 1 mg/m3 TWA: 1 mg/m3STEL: 3 ma/m3 STEL: 3 ma/m3 STEL: 3 ma/m3

Sodium hydroxide Ceilina: 2 mçi/m3 Peak: 2 ma/m3 CEV: 2 mçi/m3





Physical State

AppearanceodorOdor Threshold

LiquidClear ColorlessNo information availableNo information available.

Page 3 1 8



pHVapor Pressure

Vapor DensityViscosityBoilng Point/RangeMelting Point/RangeDecomposition temperature °C


Specific GravitySolubiltylog Pow

3.5No information available.No information available.No information available.No information available.No information available.No information available.Not applicable

No information available.No information available.No information available.No data available







Acute Toxicity





Chronic Toxicity


Page 4 1 8




Mutagenic Effects









Ecotoxicity

ComDonent Freshwater Alaae Freshwater Fish Microtox Water FleaPhosohoric acid Not listed Not listed Not listed EC50 12 h 4.6 maIL

Citric acid Not listed Leuciscus idus: LC50 = 440- Photobacterium EC50 = 120 mg/ll2h

760 mglU96h phosphoreum: EC50 = 14mglU15 min



Mobilty

ComponentCitric acid

log Pow-1.72


DOT Not regulated

TDG Not regulated

Page 5 1 8



lATA Not regulated




Phosphoric acid X X - 231-633- - X X X X KE-2 27427

X

Citric acid X X - 201-069- - X X X X KE-1 20831

X

Sodium hydroxide X X - 215-185- - X X X X KE-

5 31487X

Legend:X - ListedE - Indicates a substance that is the subject of a Section 5(e) Consent order under TSCA.F - Indicates a substance that is the subject of a Section 5(f) Rule under TSCA.N - Indicates a polymeric substance containing no free-radical initiator in it inventory name but is considered to cover the designatedpolymer made with any free-radical initiator regardless of the amount used.P - Indicates a commenced PMN substanceR - Indicates a substance that is the subject of a Section 6 risk managemnt rule under TSCA.S - Indicates a substance that is identifed in a proposed or final Signifant New Use RuleT - Indicates a substance that is the subject of a Section 4 test rule under TSCA.XU - Indicates a substance exempt from reporting under the Inventory Update Rule, i.e. Partial Updating of the TSCA Inventory Data BaseProduction and Site Report (40 CFR 710(B).

Y1 - Indicates an exempt polymer that has a number-average molecular weight of 1,000 or greater.Y2 - Indicates an exempt polymer that is a polyester and is made only from reactants included in a specified list of low concern reactants thatcomprises one of the eligibilty crieria for the exemption rule.




SARA 311/312 Hazardous CategorizationAcute Health HazardChronic Health HazardFire HazardSudden Release of Pressure Hazard

NoNoNoNo

Page 6 1 8

Thermo Fisher Scientific - SurePrepTM RNAlDNAlProtein Revision Date 25-Mar-2010Purification Kit, Column Activation and Wash Buffer

Reactive Hazard NoClean Water Act

Component CWA - Hazrdous CWA - Reportble CWA - Toxic Pollutants CWA - Priori PollutantsSubstances Quanties

Phosphoric acid X 5000 Ib - -

Sodium hydroxide X 1 000 Ib - -


OSHANot applicable


Comoonent Hazrdous Substances RQs CERCLA EHS RQs

Phosphoric acid 5000 Ib -

Sodium hvdroxide 1000 Ib -


State Right-to-Know

MassachusettXX

New JerseX

X

Ilinois Rhode IslandXX





Canada



Page 7 1 8







DisclaimerThe information provided on this Safety Data Sheet is corrct to the best of our knowledge, information and belief at the dateof its publication. The information given is designed only as a guide for safe handling, use, processing, storage,transporttion, disposal and release and is not to be considered as a warrnty or quality specification. The information


End of MSDS

Page 8 1 8

Fisher SclentlRe



Product Name SurePrepTl RNA/DNA/Protein Purification Kit, Protein pH Binding Buffer

Cat No. BP2802-50





DANGER!

&'ppearance Clear Colorless

Emergency OverviewCauses burns by all exposure routes.

Physical State Liquid odor No information available

Target Organs


Eyes, Respiratory system, Skin, Gastrointestinal tract (GI), Blood, Liver



Causes bums.Causes bums. May be harmful in contact with skin.Causes bums. May be harmful if inhaled.Causes bums. May be harmful if swallowed. Ingestion may cause gastrointestinal irritation,nausea, vomiting and diarrhea.

Chronic Effects May cause adverse liver effects.


Aggravated Medical Conditions Preexisting eye disorders. Skin disorders.

Page 1 1 8

Thermo Fisher Scientific - SurePrepTM RNAlDNAlProteinPurification Kit, Protein pH Binding Buffer


HazlNon-hazComponent CAS-No Weiaht "1

Nuclease Free Water 7732-18-5 80 - 85Phosohoric acid 7664-38-2 8 - 10

Citric acid 77 -92-9 7 - 8Sodium hvdroxide 1310-73-2 3.2






Flash PointMethod

Not applicable



UpperLower











Page 2 1 8

Thermo Fisher Scientific - SurePrepTM RNAlDNAlProteinPurifcation Kit, Protein pH Binding Buffer





Handling Use only under a chemical fume hood. Wear personal protective equipment. Do not breathevapors or spray mist. Do not get in eyes, on skin, or on clothing.

Keep containers tightly closed in a dry, cool and well-ventilated place. Corrosives area.Storage


Exposure Guidelines

ComDonent ACGIH TLV OSHA PEL NIOSH IOLH

Phosphoric acid TWA: 1 mg/m3 (Vacated) TWA: 1 mg/m3 IDLH: 1000 mg/m3STEL: 3 mg/m3 (Vacated) STEL: 3 mg/m3 TWA: 1 mg/m3

TWA: 1 ma/m3 STEL: 3 ma/m3

Sodium hydroxide Ceiling: 2 mg/m3 (Vacated) Ceiling: 2 mg/m3 IDLH: 10 mg/m3TWA: 2 mQ/m3 Ceilina: 2 mQ/m3


Phosphoric acid TWA: 1 mg/m3 TWA: 1 mg/m3 TWA: 1 mg/m3STEL: 3 mQ/m3 STEL: 3 mQ/m3 STEL: 3 mQ/m3

Sodium hydroxide Ceilina: 2 ma/m3 Peak: 2 ma/m3 CEV: 2 ma/m3





Physical State

AppearanceodorOdor Threshold

LiquidClear ColorlessNo information availableNo information available.

Page 3 1 8





pHVapor Pressure





Conditions to Avoid Incompatible products. Excess heat.

Incompatible Materials Strong oxidizing agents

Hazardous Decomposition Products Carbon monoxide (CO), Carbon dioxide (C02), Oxides ofphosphorus



Acute Toxicity


Com




Chronic Toxicity


Page 4 1 8










Ecotoxicity

ComDonent Freshwater Alaae Freshwater Fish Microtox Water FleaPhosohoric acid Not listed Not listed Not listed EC50 12 h 4.6 maIL

Citric acid Not listed Leuciscus idus: LC50 = 440- Photobacterium EC50 - 120 mg/L/2h760 mglU96h phosphoreum: EC50 = 14

mglU15 min



Mobilty Component log PowCitric acid -1.72Waste Disposal Methods Chemical waste generators must determine whether a discarded chemical is classified as a

hazardous waste. Chemical waste generators must also consult local, regional, and nationalhazardous waste regulations to ensure complete and accurate classification

DOT

UN-NoProper Shipping NameHazard ClassPacking Group

UN1805PHOSPHORIC ACID SOLUTION8III

Page 5 1 8



TDG


Packing Group

lATA


Packing Group

IMDGIIMO


Packing Group




IntemationallnventoriesComponent TSCA DSL NDSL EINECS ELiNCS NLP PICCS ENCS AICS CHINA KECL


Phosphoric acid X X - 231-633- - X X X X KE-

2 27427X


X

Sodium hydroxide X X - 215-185- - X X X X KE-

5 31487X

Legend:X - ListedE -Indicates a substance that is the subject of a Section 5(e) Consent order under TSCA.F - Indicates a substance that is the subject of a Section 5(t) Rule under TSCA.N - Indicates a polymeric substance containing no free-radical initiator in it inventory name but is considered to cover the designatedpolymer made with any free-radical initiator reardless of the amount used.P - Indicates a commenced PMN substanceR - Indicates a substance that is the subject of a Section 6 risk management rule under TSCA.S - Indicates a substance that is identifed in a proposed or final Signifcant New Use RuleT - Indicates a substance that is the subject of a Section 4 test rule under TSCA.XU -Indicates a substance exempt from reporting under the Inventory Update Rule, i.e. Partial Updating of the TSCA Inventory Data BaseProduction and Site Report (40 CFR 710(B).

Page 6 1 8








NoNoNoNoNo

Clean Water Act

Component CWA - Hazardous CWA - Reportble CWA - Toxic Pollutants CWA - Priori PollutantsSubstances Quantities


Sodium hydroxide X 1000lb - -


OSHANot applicable


ComDonent Hazrdous Substances RQs CERCLA EHS RQs

Phosphoric acid 5000 Ib -

Sodium hydroxide 1000 Ib -


State Right-to-Know

MassachusettX

X

New JerseX

X

Illnois Rhode IslandX

X


Page 7 1 8






Canada


WHMIS Hazard ClassE Corrosive material

§''"-,..,_.-







End of MSDS

Page 8 1 8

Fisher SclentlRe



Product Name SurePrepTl RNA/DNA/Protein Purification Kit, Protein Elution Buffer

Cat No. BP2802-50






Causes burns by all exposure routes.

Ilppearance Clear Colorless Physical State Liquid odor No information available

Target Organs Respiratory system, Eyes, Skin, Gastrointestinal tract (GI)




Causes bums.Causes bums. May be harmful in contact with skin.Causes bums. May be harmful if inhaled.Causes bums. May be harmful if swallowed. Ingestion may cause gastrointestinal irritation,nausea, vomiting and diarrea.




Page 1 1 8

Thermo Fisher Scientific - SurePrepTM RNAlDNAlProteinPurification Kit, Protein Elution Buffer


HazlNon-hazComDonent CAS-No Weiaht "1

Nuclease Free Water 7732-18-5 :. 98Tribasic sodium ohosohate dodecahvdrate 10101-89-0 ç: 1.0

Sodium hydroxide 1310-73-2 ç: 0.1


Wash off immediately with plenty of water for at least 15 minutes. Immediate medical attentionis required.

Skin Contact




Flash PointMethod

Not applicable



UpperLower











Page 2 1 8







Keep containers tightly closed in a dry, cool and well-ventilated place. Corrosives area.Storage


Exposure Guidelines

Com onentSodium hydroxide

ACGIH TLVCeiling: 2 mg/m3

OSHA PEL(Vacated) Ceiling: 2 mg/m3

TWA: 2 m 1m3

ComponentSodium h droxide





Physical StateAppearanceodorOdor ThresholdpHVapor Pressure

Vapor DensityViscosity

LiquidClear ColorlessNo information availableNo information available.12.5No information available.No information available.No information available.

Page 3 1 8



No information available.No information available.No information available.Not applicableNo information available.No information available.No information available.No data available

Boilng Point/Range



Specific Gravity

Solubiltylog Pow





Hazardous Polymerization

Hazardous Reactions.

Hazardous polymerization does not occur

None under normal processing.

Acute Toxicity


C i fomponent n ormationComDonent LD50 Oral LD50 Derml LC50 Inhalation

Tribasic sodium phosphate 7400 mglkg (Rat) Not listed Not listeddodecahvdrate

Sodium hydroxide Not listed 1350 mc:/kc: (Rabbit) Not listed




Chronic Toxicity



Page 4 1 8



Mutagenic Effects



Teratogenicity


Endocrine Disruptor Information








Persistence and Degradabilty

Bioaccumulation/ Accumulation

Mobilty




Waste Disposal Methods

DOT


TDG


lATA

UN-No

Chemical waste generators must determine whether a discarded chemical is classified as ahazardous waste. Chemical waste generators must also consult local, regional, and nationalhazardous waste regulations to ensure complete and accurate classification

UN3266CORROSIVE LIQUID, BASIC, INORGANIC, N.O.S.8III


UN3266

Page 5 1 8



Proper Shipping NameHazard Class

Packing Group

CORROSIVE LIQUID, BASIC, INORGANIC, N.O.S.8III

IMDGIIMO


Packing Group




Tribasic sodium phosphate - - - - - X - X X -

dodecahvdrateSodium hydroxide X X - 215-185- - X X X X KE-

5 31487X

Legend:X - ListedE - Indicates a substance that is the subject of a Section 5(e) Consent order under TSCA.F - Indicates a substance that is the subject of a Section 5(f) Rule under TSCA.N - Indicates a polymeric substance containing no free-radical initiator in it inventory name but is considered to cover the designatedpolymer made with any free-radical initiator regardless of the amount used.P - Indicates a commenced PMN substanceR - Indicates a substance that is the subject of a Section 6 risk management rule under TSCA.S - Indicates a substance that is identifed in a proposed or final Signifcant New Use RuleT - Indicates a substance that is the subject of a Section 4 test rule under TSCA.XU - Indicates a substance exempt from reporting under the Inventory Update Rule, i.e. Partial Updating of the TSCA Inventory Data BaseProduction and Site Report (40 CFR 710(B).





SARA 311/312 Hazardous CategorizationAcute Health HazardChronic Health HazardFire Hazard

YesNoNo

Page 6 1 8

Thermo Fisher Scientific - SurePrepTM RNAlDNAlProtein Revision Date 25-Mar-2010Purification Kit, Protein Elution Buffer

Sudden Release of Pressure Hazard NoReactive Hazard NoClean Water Act


Tribasic sodium phosphate X - - -

dodecahvdrateSodium hydroxide X 1000 Ib - -


OSHANot applicable


ComDonent Hazrdous Substances RQs CERCLA EHS RQs

Tribasic sodium ohosohate dodecahvdrate 5000 Ib -

Sodium hvdroxide 1000 Ib -


State Right-to-Know

ComDonent Massachusett New Jersev Pennsvlvania Ilinois Rhode Island

Tribasic sodium phosphate X - X - -

dodecahvdrateSodium hydroxide X X X - X





Canada



Page 7 1 8



§...: ~







End of MSDS

Page 8 1 8

Fisher Sclentlfle



Product Name SurePrepTl RNAIDNA/Protein Purification Kit, Protein Neutralizer

Cat No. BP2802-50






Causes burns by all exposure routes.

~ppearance Clear Colorless Physical State Liquid odor No information available

Target Organs


Eyes, Respiratory system, Skin, Gastrointestinal tract (GI), Blood, Liver



Causes bums.Causes bums. May be harmful in contact with skin.Causes bums. May be harmful if inhaled.Causes bums. May be harmful if swallowed. Ingestion may cause gastrointestinal irritation,nausea, vomiting and diarrhea.

Chronic Effects May cause adverse liver effects.


Aggravated Medical Conditions Preexisting eye disorders. Skin disorders.

Page 1 I 8

Thermo Fisher Scientific - SurePrepTM RNAlDNAlProteinPurification Kit, Protein Neutralizer



Nuclease Free Water 7732-18-5 ;. 92Sodium hvdroxide 1310-73-2 3.2

Citric acid 77-92-9 1 - 3Phosohoric acid 7664-38-2 .. 1




Ingestion

Notes to Physician

Do not induce vomiting. Call a physician or Poison Control Center immediately.

Treat symptomatically.

Flash PointMethod

Not applicable



UpperLower




Substance is nonflammable; use agent most appropriate toextinguish surrounding fire..


Suitable Extnguishing Media







Page 2 I 8







Storage Keep containers tightly closed in a dry, cool and well-ventilated place. Corrosives area.


Exposure Guidelines

Component ACGIH TLV OSHA PEL NIOSH IOLH

Sodium hydroxide Ceiling: 2 mg/m3 (Vacated) Ceiling: 2 mg/m3 IOLH: 10 mg/m3TWA: 2 ma/m3 Ceilina: 2 ma/m3

Phosphoric acid TWA: 1 mg/m3 (Vacated) TWA: 1 mg/m3 IOLH: 1000 mg/m3STEL: 3 mg/m3 (Vacated) STEL: 3 mg/m3 TWA: 1 mg/m3

TWA: 1 ma/m3 STEL: 3 mg/m3


Sodium hydroxide Ceilina: 2 mg/m3 Peak: 2 ma/m3 CEV: 2 mg/m3Phosphoric acid TWA: 1 mg/m3 TWA: 1 mg/m3 TWA: 1 mg/m3

STEL: 3 ma/m3 STEL: 3 ma/m3 STEL: 3 ma/m3





Physical StateAppearanceodorOdor Threshold

LiquidClear ColorlessNo information available


Page 3 1 8



pHVapor Pressure



Specifc Gravity

Solubiltylog Pow









Acute Toxicity


Com




Chronic Toxicity


Page 4 1 8










Ecotoxicity

Comoonent Freshwater Alaae Freshwater Fish Microtox Water FleaCitric acid Not listed Leuciscus idus: LC50 = 440- Photobacterium EC50 = 120 mg/L/2h

760 mglU96h phosphoreum: EC50 = 14mglU15 min

Phosohoric acid Not listed Not listed Not listed EC50 12 h 4.6 maIL



Mobilty Component log PowCitric acid -1.72Waste Disposal Methods Chemical waste generators must determine whether a discarded chemical is classified as a

hazardous waste. Chemical waste generators must also consult local, regional, and nationalhazardous waste regulations to ensure complete and accurate classification

1~:Vj 141J--SlTl:.

DOT


Packing Group


Page 5 1 8



TDG


Packing Group

lATA


Packing Group

IMDGIIMO


Packing Group




IntemationallnventoriesComponent TSCA DSL NDSL EINECS ELiNCS NLP PICCS ENCS AICS CHINA KECL


Sodium hydroxide X X - 215-185- - X X X X KE-5 31487

X


X

Phosphoric acid X X - 231-633- - X X X X KE-2 27427

X

Legend:X - ListedE - Indicates a substance that is the subject of a Section 5(e) Consent order under TSCA.F - Indicates a substance that is the subject of a Section 5(f) Rule under TSCA.N - Indicates a polymeric substance containing no free-radical initiator in it inventory name but is considered to cover the designatedpolymer made with any free-radical initiator regardless of the amount used.P - Indicates a commenced PMN substanceR - Indicates a substance that is the subject of a Section 6 risk management rule under TSCA.S - Indicates a substance that is identifed in a proposed or final Signifcant New Use RuleT - Indicates a substance that is the subject of a Section 4 test rule under TSCA.XU -Indicates a substance exempt from reporting under the Inventory Update Rule, i.e. Partial Updating of the TSCA Inventory Data BaseProduction and Site Report (40 CFR 710(B).

Page 6 1 8








YesNoNoNoNo

Clean Water Act


Sodium hvdroxide X 1000 Ib - -



OSHANot applicable


Comoonent Hazrdous Substances RQs CERCLA EHS RQs

Sodium hvdroxide 1000lb -

Phosphoric acid 5000lb -


State Right-to-Know

MassachusettX

X

New JerseX

X

Ilinois Rhode IslandX

X

u.s. Department of TransporttionReportable Quantity (RQ): YDOT Marine Pollutant NDOT Severe Marine Pollutant N

Page 7 1 8






Canada

This product has been classifed in accordance with the hazard criteria of the Controlled Products Regulations (CPR) and theMSDS contains all the information required by the CPR.


§







End of MSDS

Page 8 1 8

Fisher SelentlRe


Material Safety Data SheetRevision Date 25-Mar-201 0 Revision Number 1

Product Name SurePrepTl RNA/DNA/Protein Purification Kit, Loading Dye

Cat No. BP2802-50





WARNING!Emergency Overview

Causes eye, skin, and respiratory tract irritation. May cause central nervous system effects.

Appearance Dark green Physical State Liquid odor No information available

Target Organs


Eyes, Skin, Respiratory system, Central nervous system (CNS), Kidney, Blood



I rrtating to eyes.Irrtating to skin. May be harmful in contact with skin.Irrtating to respiratory system. May be harmful if inhaled.May be harmful if swallowed. May cause central nervous system effects. Ingestion may causegastrointestinal irritation, nausea, vomiting and diarrhea.

Chronic Effects Tumorigenic effects have been reported in experimental animals.. Experiments have shownreproductive toxicity effects on laboratory animals. May cause adverse kidney effects.


Aggravated Medical Conditions Preexisting eye disorders. Kidney disorders. Skin disorders.

Page 1 1 8

Thermo Fisher Scientific - SurePrepTM RNAlDNAlProteinPurification Kit, Loading Dye



Glvcerin 56-81-5 401,3-Propanediol, 2-amino-2-(hydroxymethyl)-, 1185-53-1 23 - 25

hvdrochlorideNuclease Free Water 7732-18-5 16 - 22

2,3-Butanediol, 1,4-dimercaoto-, (R* ,R*)- 3483-12-3 7 - 9Glvcine, N-methvl-N-(1-oxododecvll, sodium salt 137-16-6 7 - 9

Bromphenol blue 115-39-9 .: 1.0


Skin Contact Wash off immediately with plenty of water for at least 15 minutes. Obtain medical attention.

Inhalation Move to fresh air. If breathing is diffcult, give oxygen. Obtain medical attention.



Flash PointMethod



UpperLower



Suitable Extinguishing Media Use water spray, alcohol-resistant foam, dry chemical or carbondioxide.








Page 2 1 8




Should not be released into the environment.Environmental Precautions


Handling Wear personal protective equipment. Ensure adequate ventilation. Do not breathe vapors orspray mist. Do not get in eyes, on skin, or on clothing.



Exposure Guidelines

ComDonent ACGIH TLV OSHA PEL NIOSH IOLH

Glycerin TWA: 10 mg/m3 (Vacated) TWA: 10 mg/m3(Vacated) TWA: 5 mg/m3

TWA: 15 mg/m3TWA: 5 ma/m3

ComponentGI cerin





Physical State


Vapor Density

LiquidDark greenNo information availableNo information available.6.8No information available.No information available.

Page 3 1 8



ViscosityBoilng Point/Range




No information available.No information available.No information available.No information available.Not applicable





Hazardous Decomposition Products Carbon monoxide (CO), Carbon dioxide (C02)



Acute Toxicity


Irritation Irrtating to eyes, respiratory system and skin

No information available.Toxicologically Synergistic

Products

Chronic Toxicity



Mutagenic Effects Mutagenic effects have occurred in humans.

Page 4 1 8





Teratogenicity





Tumorigenic effcts have been reported in experimental animals.. See actual entry in RTECSfor complete information.


Ecotoxicity


MicrotoxNot listed

Water FleaEC50 24 h ~500 m IL



Mobilty

10 Pow-1.766.77


DOT Not regulated

TDG Not regulated

lATA Not regulated


Page 5 1 8




Glycerin X X - 200-289- - X X X X KE-5 29297

X1 ,3-Propanediol, 2-amino-2- X X - 214-684- - X - X X KE-

(hydroxymethyl)-, 5 34819hydrochloride X


2,3-Butanediol, 1,4- X X - 222-468- - X - X X -

dimercapto-, (R*, R*)- 7Glycine, N-methyl-N-( 1- X X - 205-281- - X X X X KE-

oxododecyl)-, sodium salt 5 21878X

Bromphenol blue X X - 204-086- - X X X X KE-2 02746

X

Legend:X - ListedE - Indicates a substance that is the subject of a Section 5(e) Consent order under TSCA.F - Indicates a substance that is the subject of a Section 5(f) Rule under TSCA.N - Indicates a polymeric substance containing no free-radical initiator in it inventory name but is considered to cover the designatedpolymer made with any free-radical initiator regardless of the amount used.P - Indicates a commenced PMN substanceR - Indicates a substance that is the subject of a Section 6 risk management rule under TSCA.S -Indicates a substance that is identifed in a proposed or final Signifcant New Use RuleT - Indicates a substance that is the subject of a Section 4 test rule under TSCA.XU - Indicates a substance exempt from reporting under the Inventory Update Rule, i.e. Partial Updating of the TSCA Inventory Data BaseProduction and Site Report (40 CFR 710(B).

Y1 - Indicates an exempt polymer that has a number-average molecular weight of 1,000 or greater.Y2 - Indicates an exempt polymer that is a polyester and is made only from reactants included in a specified list of low concern reactants thatcomprises one of the eligibilit criteria for the exemption rule.





YesNoNoNoNo


Page 6 1 8




OSHANot applicable



State Right-to-Know

MassachusettX

New JerseX

Ilinois Rhode IslandX

U.S. Department of TransportationReportable Quantity (RQ): NDOT Marine Pollutant NDOT Severe Marine Pollutant N




Canada


WHMIS Hazard ClassD2B Toxic materials

CD


Page 7 I 8





Revision Summary "..." , and red text indicates revision



End of MSDs

Page 8 1 8

Documents

Fisher SclentlRe - University of Nevada, RenoFisher SclentlRe Creation Date 25-Mar-2010 Material Safety Data Sheet Revision Date 25-Mar-2010 Revision Number 1 Product Name SurePrepTl