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First insights into segregation for aggressiveness among sexual progeny isolates of Puccinia striiformis
Chris K. Sørensen, Julián Rodríguez-Algaba, Mogens S. Hovmøller, Annemarie F. Justesen
Department of Agroecology, Aarhus University, Research Center Flakkebjerg, Forsøgsvej 1 – 4200 Slagelse, Denmark
14th International Cereal Rust and Powdery Mildew Conference 5-8 July 2015
A A R H U S U N I V E R S I T Y
Outline
• Experimental system for sexual reproduction and
the genetic diversity in sexual progenies.
• The definition and role of pathogen aggressiveness
• Assessment of aggressiveness for P. striiformis
• Preliminary results for segregation of aggressiveness
14th International Cereal Rust and Powdery Mildew Conference 5-8 July 2015
Yue Jin, AU-Flakkebjerg Nov. 2012
14th International Cereal Rust and Powdery Mildew Conference 5-8 July 2015
Barberry – an alternate host of P. striiformis
14th International Cereal Rust and Powdery Mildew Conference 5-8 July 2015
An experimental system for barberry infections
Germination of teliospores Inoculation of Barberry
Pycniospores Aecidiospores
Fertilization
Inoculation of wheat
Selfing of an isolate of the Warrior race
14th International Cereal Rust and Powdery Mildew Conference 5-8 July 2015
Dominating race in Europe since 2011 Readily teliospores
Production
14th International Cereal Rust and Powdery Mildew Conference 5-8 July 2015
Genetic diversity in warrior progeny
SSR marker diversity
Aggressiveness – Quantitative pathogenicity
14th International Cereal Rust and Powdery
Mildew Conference 5-8 July 2015
Does aggressiveness matter? • Several examples where replacement of old populations with new
strains could not be explained by virulence alone. Important question: • Heritability of aggressiveness? • Genetic correlation between aggressiveness traits – possible trade-off
to prevent super aggressive genotypes?
Monocyclic epidemiological components • Germination rate • Infection efficiency • Latent period • Lesion growth rate • Spore production
“Relative ability of a virulent isolate to cause disease on a susceptible host”
Setup for assessment of aggressiveness in P. striiformis
14th International Cereal Rust and Powdery Mildew Conference 5-8 July 2015
14th International Cereal Rust and Powdery Mildew Conference 5-8 July 2015
Assessment of latent period and lesion growth
Latent period
Four days
Lesion growth First marking
Four days
Lesion growth Second marking
14th International Cereal Rust and Powdery Mildew Conference 5-8 July 2015
Latent period and lesion growth on cv. Avocet.S Lesion growth
0,0 1,0 2,0 3,0 4,0 5,0 6,0 7,0 8,0 9,0
10,0 11,0 12,0
A9
A
7
A1
3
A1
2
A6
A
2
A2
2
DK
09
/11
A
19
A
15
A
18
A
5
A1
6
A1
4
A8
A
10
A
3
A4
0,0 1,0 2,0 3,0 4,0 5,0 6,0 7,0 8,0 9,0
10,0 11,0 12,0 13,0 14,0
A9
A
12
A
22
A
7
DK
09
/11
A
6
A5
A
18
A
19
A
13
A
2
A1
5
A1
6
A8
A
10
A
14
A
3
A4
0,0 1,0 2,0 3,0 4,0 5,0 6,0 7,0 8,0 9,0
10,0 11,0 12,0 13,0 14,0 15,0
A6
A
13
A
12
A
7
DK
09
/11
A
9
A2
A
22
A
14
A
5
A8
A
15
A
19
A
16
A
18
A
10
A
3
A4
mm
in 4
day
s
Exp 1.
Exp 2.
Exp 3.
Latent period
0 25 50 75
100 125 150 175 200 225 250 275 300
A1
6
A3
A
4
A2
2
A5
A
14
A
19
A
8
A1
0
A1
8
DK
09
/11
A
2
A1
5
A1
3
A1
2
A7
A
6
A9
0
25 50 75
100 125 150 175 200 225 250 275 300 325
A3
A
14
A
15
A
2
A2
2
DK
09
/11
A
19
A
5
A1
0
A1
6
A4
A
18
A
8
A1
3
A9
A
12
A
6
A7
0 25 50 75
100 125 150 175 200 225 250 275 300 325
A3
A
4
A1
9
A2
A
15
A
5
DK
09
/11
A
10
A
22
A
16
A
14
A
18
A
8
A1
2
A6
A
13
A
7
A9
Late
nt
per
iod
I h
ou
rs
14th International Cereal Rust and Powdery Mildew Conference 5-8 July 2015
Latent period and lesion growth on cv. Avocet.S Latent period
Exp 1.
Exp 2.
0 25 50 75
100 125 150 175 200 225 250 275 300
A1
6
A3
A
4
A2
2
A5
A
14
A
19
A
8
A1
0
A1
8
DK
09
/11
A
2
A1
5
A1
3
A1
2
A7
A
6
A9
0
25 50 75
100 125 150 175 200 225 250 275 300 325
A3
A
14
A
15
A
2
A2
2
DK
09
/11
A
19
A
5
A1
0
A1
6
A4
A
18
A
8
A1
3
A9
A
12
A
6
A7
0 25 50 75
100 125 150 175 200 225 250 275 300 325
A3
A
4
A1
9
A2
A
15
A
5
DK
09
/11
A
10
A
22
A
16
A
14
A
18
A
8
A1
2
A6
A
13
A
7
A9
Exp 3.
Lesion growth
0,0 1,0 2,0 3,0 4,0 5,0 6,0 7,0 8,0 9,0
10,0 11,0 12,0
A9
A
7
A1
3
A1
2
A6
A
2
A2
2
DK
09
/11
A
19
A
15
A
18
A
5
A1
6
A1
4
A8
A
10
A
3
A4
0,0 1,0 2,0 3,0 4,0 5,0 6,0 7,0 8,0 9,0
10,0 11,0 12,0 13,0 14,0
A9
A
12
A
22
A
7
DK
09
/11
A
6
A5
A
18
A
19
A
13
A
2
A1
5
A1
6
A8
A
10
A
14
A
3
A4
0,0 1,0 2,0 3,0 4,0 5,0 6,0 7,0 8,0 9,0
10,0 11,0 12,0 13,0 14,0 15,0
A6
A
13
A
12
A
7
DK
09
/11
A
9
A2
A
22
A
14
A
5
A8
A
15
A
19
A
16
A
18
A
10
A
3
A4
mm
in 4
day
s
Late
nt
per
iod
I h
ou
rs
14th International Cereal Rust and Powdery Mildew Conference 5-8 July 2015
Latent period and lesion growth on cv. Avocet.S Latent period
Exp 1.
Exp 2.
0 25 50 75
100 125 150 175 200 225 250 275 300
A1
6
A3
A
4
A2
2
A5
A
14
A
19
A
8
A1
0
A1
8
DK
09
/11
A
2
A1
5
A1
3
A1
2
A7
A
6
A9
0
25 50 75
100 125 150 175 200 225 250 275 300 325
A3
A
14
A
15
A
2
A2
2
DK
09
/11
A
19
A
5
A1
0
A1
6
A4
A
18
A
8
A1
3
A9
A
12
A
6
A7
0 25 50 75
100 125 150 175 200 225 250 275 300 325
A3
A
4
A1
9
A2
A
15
A
5
DK
09
/11
A
10
A
22
A
16
A
14
A
18
A
8
A1
2
A6
A
13
A
7
A9
Exp 3.
Lesion growth
0,0 1,0 2,0 3,0 4,0 5,0 6,0 7,0 8,0 9,0
10,0 11,0 12,0
A9
A
7
A1
3
A1
2
A6
A
2
A2
2
DK
09
/11
A
19
A
15
A
18
A
5
A1
6
A1
4
A8
A
10
A
3
A4
0,0 1,0 2,0 3,0 4,0 5,0 6,0 7,0 8,0 9,0
10,0 11,0 12,0 13,0 14,0
A9
A
12
A
22
A
7
DK
09
/11
A
6
A5
A
18
A
19
A
13
A
2
A1
5
A1
6
A8
A
10
A
14
A
3
A4
0,0 1,0 2,0 3,0 4,0 5,0 6,0 7,0 8,0 9,0
10,0 11,0 12,0 13,0 14,0 15,0
A6
A
13
A
12
A
7
DK
09
/11
A
9
A2
A
22
A
14
A
5
A8
A
15
A
19
A
16
A
18
A
10
A
3
A4
mm
in 4
day
s
Late
nt
per
iod
I h
ou
rs
14th International Cereal Rust and Powdery Mildew Conference 5-8 July 2015
Latent period and lesion growth on cv. 514W
Late
nt
per
iod
I h
ou
rs
Exp 1.
Latent period
Exp 2.
Exp 3.
0 25 50 75
100 125 150 175 200 225 250 275 300 325
A1
6
A1
8
A2
2
A4
A
8
A1
0
A1
9
A3
A
14
A
15
A
5
DK
09
/11
A
6
A2
A
13
A
12
A
7
A9
0
25 50 75
100 125 150 175 200 225 250 275 300 325
A1
0
A2
2
A1
6
A1
9
A1
5
A1
8
A2
A
14
A
3
DK
09
/11
A
5
A4
A
13
A
8
A9
A
6
A1
2
A7
0 25 50 75
100 125 150 175 200 225 250 275 300 325
A3
A
4
A2
2
A1
9
A2
A
5
A1
5
A1
8
DK
09
/11
A
10
A
16
A
8
A1
4
A9
A
6
A7
A
13
A
12
Lesion growth
0,0 1,0 2,0 3,0 4,0 5,0 6,0 7,0 8,0 9,0
10,0 11,0 12,0
A6
A
9
A1
2
A2
2
A5
A
13
A
7
A1
9
A1
4
A1
8
A1
6
DK
09
/11
A
8
A1
0
A1
5
A2
A
3
A4
0,0 1,0 2,0 3,0 4,0 5,0 6,0 7,0 8,0 9,0
10,0 11,0 12,0 13,0
A9
A
6
A2
2
A7
A
5
A1
2
A1
3
A2
A
19
A
8
DK
09
/11
A
15
A
18
A
16
A
10
A
14
A
3
A4
0,0 1,0 2,0 3,0 4,0 5,0 6,0 7,0 8,0 9,0
10,0 11,0 12,0 13,0 14,0 15,0 16,0
A9
A
12
A
6
DK
09
/11
A
13
A
16
A
14
A
5
A2
2
A7
A
8
A1
9
A1
0
A1
8
A2
A
15
A
4
A3
mm
in 4
day
s
Correlation between latent period and lesion growth
0,0
1,0
2,0
3,0
4,0
5,0
6,0
7,0
8,0
9,0
10,0
11,0
12,0
13,0
220 230 240 250 260 270 280 Le
sio
n g
row
th
Latent period
0,0
1,0
2,0
3,0
4,0
5,0
6,0
7,0
8,0
9,0
10,0
11,0
12,0
13,0
230 240 250 260 270 280 290
Lesi
on
gro
wth
Latent period
514W Avocet.S
14th International Cereal Rust and Powdery Mildew Conference 5-8 July 2015
Chlorotic phenotype
10 19 4 3 12 18 15 2 8 20 11 1 14 17 7 5 6 9 16 13
12 dpi
10 19 4 3 12 18 15 2 8 20 11 1 14 17 7 5 6 9 16 13
19 dpi
14th International Cereal Rust and Powdery Mildew Conference 5-8 July 2015
Conclusion
• A new system for simple and repeatable assessment of components of aggressiveness for P. striiformis on wheat seedlings.
• Segregation for both latent period and lesion growth in progeny isolates from a selfing of an isolate of the warrior race • Indications of a trade-off effect between latent period and lesion growth. • Similar results on two susceptible wheat varieties with two very different genetic backgrounds.
Acknowledgements
A A R H U S U N I V E R S I T Y
14th International Cereal Rust and Powdery Mildew Conference 5-8 July 2015
Mogens S. Hovmøller Annemarie F. Justesen Julian Rodriguez-Algaba Mehran Patpour Sajid Ali Tine Thach Yan-Jun Chen Ellen Frederiksen Steen Meier Janne Hansen
Claude de Vallavieille-Pope Marc Leconte
Partners
Financial support
