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FINDING 16S
BY JADINE DALEY
New Domain
16S rRNA
rRNA Found in all Bacteria Functional role is same in all ribosomes Structure changes slowly with time Contains variable and conserved regions
Used as standard for classifying and identifying microbes
Technique pioneered by Carl Woese and George E. Fox in 1977
16S in PCR
In our PCR experiment we...
Mixed our purified DNA with Primers and Master Mix to target 16S rRNA gene
Placed in thermocycler to amplify
Loaded amplified DNA into gel
Ran Gel Electrophoresis
Visualized gel
Celia Moore, PhDProfessor of Psychology, College
of Liberal Arts
Areas of Expertise
Developmental Psychobiology
Behavioral Neuroscience
Hormones and Behavior
Sexual Differentiation