SOLID STATE FERMENTATION OFPADDY STRAW FOR PRODUCTION OF BIOETHANOL USING TRICHODERMA

REESEISANDHYA VARMA S. AND A.MURALIDHARAN

DEPARTMENT OF GENETIC ENGINEERING, SRM UNIVERSITY

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INTRODUCTION• Recently the demand of ethanol has increased considerably

because of its use as gasoline in addition to other application in industries which need production of alcohol on large scale ( Wati L et al.,2007)

• Abundant agricultural residue such as corn stover, wheat straw, rice straw, bagasse, etc. are used in cellulase production (Romero et al., 1999; Xia and Cen,1999; Kang et al., 2004; Wen et al., 2005; Sukumaran et al.,2009).

• Bioethanol is a clean, renewable, low green house gas emitting fuel (Wheals AE et al., 1999)

• Ethanol has a octane number of 99 which prevents pre ignition and thereby proves to be a better fuel than petrol (Wheals AE et al.,1999)

OBJECTIVES

• Cellulolysis of paddy straw using Trichoderma reesei (MTCC*164)

• Extraction of crude extract• Distillation of crude to extract ethanol• Confirmation and Quantitative analysis of

ethanol using Gas Chromatography

MATERIALS AND METHODSMaterials:• Trichoderma reesei (MTCC*164) procured from

IMTECH, Punjab, India.• Paddy straw collected from Potheri• Solid State Fermentation set up.• Gas chromatogram (Chemito GC 1000)• Simple Distillation UnitMethods:• Solid state fermentation• Simple Distillation• Gas Chromatography

RESULTS AND DISCUSSION

Pictures showing solid state fermentation of paddy straw

Pictures showing slants with Trichoderma reesei

T. reesei

OBJECTIVE 1: Cellulolysis of paddy straw using Trichoderma reesei (MTCC*164)

Complete hydrolysis

Partial hydrolysis

Incomplete hydrolysis

Growth of T.reesei in the walls of the bioreactor

OBJECTIVE 2: Extraction of crude extract

Pictures showing the extraction of crude extract

Crude extract

OBJECTIVE 3:Distillation to extract ethanol

Cooling system

Crude extract

Distillate

Picture showing the distillate after distillation

Ethanol

Hexane layer

water

Picture showing the separation of ethanol using hexane layer

Extraction of ethanol by solvent extraction method

10% ethanol standard 20% ethanol standard Chromatogram showing peaks for ethanol standards

30% ethanol standard 40% ethanol standard

Chromatogram showing peaks for ethanol sandards

Chromatogram showing peak for the sample ethanol

OBJECTIVE 4: Quantification of ethanol using Gas Chromatography

TABLE 1Sample Substrate

weight(grams)Equivalent broth(ml)

Duration in days

SSF1 (PS Tr) 40 40 63

SSF2 (PS Tr) 30 30 61

SSF3 (PS Tr) 20 20 57

Sample yield of crude extract (ml)

Extract after distillation(ml)

yield of bioethanol from distillate(%)(v/v)

Total yield of bioethanol (%)(v/v)

SSF1 (PS Tr) 60 07 ml 16 2.8

SSF2 (PS Tr) 30 03 ml 15.6 2.7

SSF3 (PS Tr) 20 05 ml 15.5 2.6

TABLE 2

STATUS REPORT

OBJECTIVES STATUS

Cellulolysis of paddy straw using Trichoderma reesei MTCC*164

completed

Extraction of crude extract completed

Distillation to extract ethanol. completed

Quantification of ethanol using Gas Chromatography

completed

THANK YOU!!!