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File: 28012003.001 Sample: Celulas Dendriticas Beads1
Date: 28-Jan-3 Parameters: 5
Total Events 14220 Gated Events 1962 13.80%
System: Log Parameter Means: Geometric
Quad Stats
FL1-Height(Log) vs Empty(Log)
Quadrant x,y: 824,880
Quad X-Mean Y-Mean Events %Total %Gated
1 UL 1591.6 3059.7 3 0.02 0.15
2 UR 2567.5 4061.9 6 0.04 0.31
3 LL 270.8 415.3 1948 13.70 99.29
4 LR 2423.1 2038.8 5 0.04 0.25
File: 28012003.004 Sample: Celulas Dendriticas Beads1
Date: 28-Jan-3 Parameters: 5
Total Events 10950 Gated Events 2031 18.55%
System: Log Parameter Means: Geometric
Quad Stats
FL1-Height(Log) vs Empty(Log)
Quadrant x,y: 660,652
Quad X-Mean Y-Mean Events %Total %Gated
1 UL 257.1 368.5 1 0.01 0.05
2 UR 7802.4 1686.2 431 3.94 21.22
3 LL 115.4 85.1 364 3.32 17.92
4 LR 6783.5 14.9 1235 11.28 60.81
1a analise beads
File: 28012003.004 Sample: Celulas Dendriticas Beads1
Date: 28-Jan-3 Parameters: 1
Total Events 10950 Gated Events 2031 18.55%
System: Log Parameter Means: Geometric
Param name M Low,High Events %Total %Gated GMean CV Peak,Value
FL1-Heigh 0 0,1023 2031 18.55 100.00 3361.86 19.92 1234,8058.42
1 804,1023 1653 15.10 81.39 7166.20 2.72 1234,8058.42
File: 28012003.003 Sample: Celulas Dendriticas Beads1
Date: 28-Jan-3 Parameters: 5
Total Events 15000 Gated Events 2178 14.52%
System: Log Parameter Means: Geometric
Quad Stats
FL1-Height(Log) vs Empty(Log)
Quadrant x,y: 824,880
Quad X-Mean Y-Mean Events %Total %Gated
1 UL 0.0 0.0 0 0.00 0.00
2 UR 8058.4 4858.7 4 0.03 0.18
3 LL 225.3 324.4 2105 14.03 96.65
4 LR 5591.3 543.2 69 0.46 3.17
File: 28012003.002 Sample: Celulas Dendriticas Beads1
Date: 28-Jan-3 Parameters: 5
Total Events 15000 Gated Events 4172 27.81%
System: Log Parameter Means: Geometric
Quad Stats
FL1-Height(Log) vs Empty(Log)
Quadrant x,y: 884,892
Quad X-Mean Y-Mean Events %Total %Gated
1 UL 0.0 0.0 0 0.00 0.00
2 UR 6871.5 6019.7 7 0.05 0.17
3 LL 650.8 653.9 4092 27.28 98.08
4 LR 5467.0 718.4 73 0.49 1.75
File: 28012003.005 Sample: Celulas Dendriticas Beads1
Date: 28-Jan-3 Parameters: 5
Total Events 30000 Gated Events 6523 21.74%
System: Log Parameter Means: Geometric
Quad Stats
FL1-Height(Log) vs Empty(Log)
Quadrant x,y: 824,880
Quad X-Mean Y-Mean Events %Total %Gated
1 UL 0.0 0.0 0 0.00 0.00
2 UR 8051.4 4570.3 543 1.81 8.32
3 LL 191.0 208.9 1404 4.68 21.52
4 LR 7047.3 109.3 4576 15.25 70.15
File: 28012003.005 Sample: Celulas Dendriticas Beads1
Date: 28-Jan-3 Parameters: 1
Total Events 30000 Gated Events 6523 21.74%
System: Log Parameter Means: Geometric
Param name M Low,High Events %Total %Gated GMean CV Peak,Value
FL1-Heigh 0 0,1023 6523 21.74 100.00 3277.52 19.18 3796,8058.42
1 830,1023 5115 17.05 78.41 7155.60 2.71 3796,8058.42
File: 14022003.001 Sample: CD beads
Date: 14-Feb-3 Parameters: 5
Total Events 15000 Gated Events 1596 10.64%
System: Log Parameter Means: Geometric
Quad Stats
Empty(Log) vs Empty(Log)
Quadrant x,y: 700,664
Quad X-Mean Y-Mean Events %Total %Gated
1 UL 0.0 0.0 0 0.00 0.00
2 UR 0.0 0.0 0 0.00 0.00
3 LL 46.4 32.5 1596 10.64 100.00
4 LR 0.0 0.0 0 0.00 0.00
Total Events 30000 Gated Events 4477 14.92%
System: Log Parameter Means: Geometric
Quad Stats
Empty(Log) vs Empty(Log)
Quadrant x,y: 676,636
Quad X-Mean Y-Mean Events %Total %Gated
1 UL 0.0 0.0 0 0.00 0.00
2 UR 8002.9 721.7 411 1.37 9.18
3 LL 62.7 28.0 2073 6.91 46.30
4 LR 4438.6 119.5 1993 6.64 44.52
2a analise beads
Multiple Document Interface for Flow Cytometry
WinMDI Version 2.8 - Windows 3.10/DOS 5.0
Tue Feb 18 14:18:01 2003
Gates: R1
Project: Experiment:
File: 14022003.002 Sample: CD beads
Date: 14-Feb-3 Parameters: 1
Total Events 30000 Gated Events 4477 14.92%
System: Log Parameter Means: Geometric
Param name M Low,High Events %Total %Gated GMean CV Peak,Value
Empt 0 0,1023 4477 14.92 100.00 651.63 34.74 867,8058.42
1 803,1023 2276 7.59 50.84 5400.26 5.22 867,8058.42+
File: 27012003.001 Sample: Cels. Dendriticas cultura 1
Date: 27-Jan-3 Parameters: 5
Total Events 22220 Gated Events 1900 8.55%
System: Log Parameter Means: Geometric
Quad Stats
Empty(Log) vs FL2-Height(Log)
Quadrant x,y: 736,800
Quad X-Mean Y-Mean Events %Total %Gated
1 UL 372.2 1795.5 28 0.13 1.47
2 UR 2978.2 3158.0 33 0.15 1.74
3 LL 260.2 433.2 1822 8.20 95.89
4 LR 930.1 907.2 17 0.08 0.89
File: 27012003.003 Sample: Cels. Dendriticas cultura 1
Date: 27-Jan-3 Parameters: 5
Total Events 21044 Gated Events 1725 8.20%
System: Log Parameter Means: Geometric
Quad Stats
CD11c PE(Log) vs Empty(Log)
Quadrant x,y: 736,800
Quad X-Mean Y-Mean Events %Total %Gated
1 UL 0.0 0.0 0 0.00 0.00
2 UR 3100.6 2915.1 21 0.10 1.22
3 LL 282.7 141.0 1536 7.30 89.04
4 LR 1230.1 214.6 168 0.80 9.74
1a analise cd11c
File: 27012003.003 Sample: Cels. Dendriticas cultura 1
Date: 27-Jan-3 Parameters: 1
Total Events 21044 Gated Events 1725 8.20%
System: Log Parameter Means: Geometric
Param name M Low,High Events %Total %Gated GMean CV Peak,Value
CD11c P 0 0,1023 1725 8.20 100.00 335.87 11.75 19,235.014
1 777,1023 101 0.48 5.86 1985.73 7.93 9,8058.42
2 analise marcação cd11b, cd11c
File: 21032003.002 Sample: Celulas dendriticas Control
Date: 21-Mar-3 Parameters: 5
Total Events 40000 Gated Events 3612 9.03%
System: Log Parameter Means: Geometric
Quad Stats
Mouse IgG1 FITC(Log) vs Mouse IgG1 PE(Log)
Quadrant x,y: 404,400
Quad X-Mean Y-Mean Events %Total %Gated
1 UL 25.9 39.5 10 0.03 0.28
2 UR 87.9 58.3 16 0.04 0.44
3 LL 9.1 7.4 3566 8.91 98.73
4 LR 44.6 23.8 20 0.05 0.55
File: 21032003.003 Sample: Celulas dendriticas
Date: 21-Mar-3 Parameters: 5
Total Events 45267 Gated Events 2321 5.13%
System: Log Parameter Means: Geometric
Quad Stats
CD11b FITC(Log) vs CD11c PE(Log)
Quadrant x,y: 404,400
Quad X-Mean Y-Mean Events %Total %Gated
1 UL 0.0 0.0 0 0.00 0.00
2 UR 262.4 142.0 22 0.05 0.95
3 LL 16.2 7.2 207 0.46 8.92
4 LR 281.9 2.5 2092 4.62 90.13
Project: Experiment:
File: 21032003.003 Sample: Celulas dendriticas
Date: 21-Mar-3 Parameters: 1
Total Events 45267 Gated Events 2321 5.13%
System: Log Parameter Means: Geometric
Param name M Low,High Events %Total %Gated GMean CV Peak,Value
CD11b FIT 0 0,1023 2321 5.13 100.00 218.31 20.02 20,410.47
1 418,1023 2096 4.63 90.31 286.45 12.40 20,410.47
File: 21032003.004 Sample: Celulas dendriticas
Date: 21-Mar-3 Parameters: 5
Total Events 40000 Gated Events 2369 5.92%
System: Log Parameter Means: Geometric
Quad Stats
CD11b FITC(Log) vs FL2-Height(Log)
Quadrant x,y: 404,400
Quad X-Mean Y-Mean Events %Total %Gated
1 UL 0.0 0.0 0 0.00 0.00
2 UR 137.5 52.5 9 0.02 0.38
3 LL 17.6 7.0 275 0.69 11.61
4 LR 181.8 3.0 2085 5.21 88.01
File: 21032003.004 Sample: Celulas dendriticas
Date: 21-Mar-3 Parameters: 1
Total Events 40000 Gated Events 2369 5.92%
System: Log Parameter Means: Geometric
Param name M Low,High Events %Total %Gated GMean CV Peak,Value
CD11b FIT 0 0,1023 2369 5.92 100.00 138.46 19.46 20,257.132
1 411,1023 2079 5.20 87.76 183.61 11.54 20,257.132
File: 21032003.005 Sample: Celulas dendriticas
Date: 21-Mar-3 Parameters: 5
Total Events 40000 Gated Events 3608 9.02%
System: Log Parameter Means: Geometric
Quad Stats
CD11c PE(Log) vs FL1-Height(Log)
Quadrant x,y: 404,400
Quad X-Mean Y-Mean Events %Total %Gated
1 UL 19.7 61.4 31 0.08 0.86
2 UR 52.9 68.3 27 0.07 0.75
3 LL 8.2 9.8 3547 8.87 98.31
4 LR 96.8 20.1 3 0.01 0.08
File: 21032003.005 Sample: Celulas dendriticas
Date: 21-Mar-3 Parameters: 1
Total Events 40000 Gated Events 3608 9.02%
System: Log Parameter Means: Geometric
Param name M Low,High Events %Total %Gated GMean CV Peak,Value
CD11c P 0 0,1023 3608 9.02 100.00 8.39 29.68 31,8.4291
1 405,1023 30 0.08 0.83 56.15 10.16 2,49.1367
