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Fig. S1
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GFP WT Mutant TIMP-1 TIMP-1
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Supplementary data, Liu et al., 2007
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Fig. S1. Quantitative analysis of vessel formation by relative total length. (A) Fibroblast-conditioned media promotes vessel formation. (B) Exogenous TIMP-1 enhances vessel formation. (C) Removal of TIMP-1 by siRNA from fibroblasts reduces conditioned-media induced vessel formation. (D) TIMP-1 promotes vessel formation in a MMP-dependent manner. (E) Inhibition of MMP-2/9 enhances vessel formation. (F) TIMP-1-enhanced vessel formation is abolished by active MMP-9. *P < 0.05, **P < 0.01 compared to respective control.
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Fig. S2
Supplementary data, Liu et al., 2007
Fig. S2. TIMP-1 does not affect proliferation and migration of HUVECs. (A) HUVECs were cultured in a 96-well plate and incubated without (con) or with TIMP-1 (400ng/ml). At indicated time points cell number was determined using methylene blue assay. (B-E) HUVECs were plated in 24-well plate and migration was determined by wound healing assay. The monolayers were wounded and incubated without (con) (B and C) or with TIMP-1 (400ng/ml) (D and E).
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Fig. S3
Supplementary data, Liu et al., 2007
Fig. S3. Inhibition of MMP-2/9 enhances vessel formation. HUVECs were grown inthree dimensional collagen gel for five days without (con) or with indicated concentrations of MMP-2/9 inhibitor. **P < 0.01 compared to control.
