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FIELD TRIAL REPORT
Efficacy and selectivity of Anolyte (AcquAttiva®) to control Erwinia amylovora
causing fireblight on BIO pear
PROTOCOL ID:1062018G_B SATA TRIAL ID: 1062018G_B_01
Sponsor: Bio Fruit Service Via Savigliano, 75/A 12024 Costigliole Saluzzo (CN) - Acqua Attiva 20875 Burago di Molgora (MB)
Quargnento, 28/11/2019
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PROTOCOL ID: 1062018G_B SATA TRIAL ID: 1062018G_B_01
Efficacy and selectivity of Anolyte (AcquAttiva®) to control Erwinia amylovora causing fireblight on
BIO pear
Pag. 2 di 38
Pagina 2 di 38
SATA S.r.l. – Strada Alessandria, 13 – 15044 Quargnento (AL) - Tel 0131219925 - Fax 0131219926 –
e-mail: [email protected] – web: www.satasrl.it
CONTENTS
CONTENTS .................................................................................................................................... 1
1 GEP COMPLIANCE STATEMENT ........................................................................................... 3
2 GEP OFFICIAL ACCREDITATION ........................................................................................... 4
3 ARCHIVING STATEMENT ....................................................................................................... 6
4 SUMMARY ............................................................................................................................... 6
5 TRIAL INFORMATION & METHODS ....................................................................................... 7
5.1 TRIAL TREATMENTS ............................................................................................................ 7 5.2 GENERAL TRIAL INFORMATION ............................................................................................ 7 5.3 TRIAL LOCATION ................................................................................................................. 7 5.4 CONTACTS ......................................................................................................................... 8 5.5 COOPERATOR/LANDOWNER ................................................................................................ 8 5.6 CROP DESCRIPTION ........................................................................................................... 8 5.7 PEST DESCRIPTION ............................................................................................................ 8 5.8 SITE AND DESIGN ............................................................................................................... 9 5.9 FIELD PLOT LAYOUT ............................................................................................................ 9 5.10 APPLICATION DESCRIPTION ............................................................................................... 10 5.11 CROP STAGE AT EACH APPLICATION ................................................................................. 10 5.12 PEST STAGE AT EACH APPLICATION .................................................................................. 11 5.13 APPLICATION EQUIPMENT ................................................................................................. 11
6 MOISTURE AND WEATHER CONDITIONS .......................................................................... 12
7 STATISTICAL ANALYSIS METHODS .................................................................................... 12
8 RESULTS ............................................................................................................................... 13
8.1 EFFICACY ......................................................................................................................... 13 8.2 PHYTOTOXICITY ................................................................................................................ 13
9 FINAL REMARKS................................................................................................................... 14
10 ANNEX ............................................................................................................................... 15
10.1 RAW DATA ........................................................................................................................ 15 10.2 RAW WEATHER DATA ........................................................................................................ 25 10.3 CHARTS ........................................................................................................................... 27 10.4 STATISTICAL ANALYSIS ...................................................................................................... 28 10.5 TRIAL SITE LOCATION ........................................................................................................ 36
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PROTOCOL ID: 1062018G_B SATA TRIAL ID: 1062018G_B_01
Efficacy and selectivity of Anolyte (AcquAttiva®) to control Erwinia amylovora causing fireblight on
BIO pear
Pag. 3 di 38
Pagina 3 di 38
SATA S.r.l. – Strada Alessandria, 13 – 15044 Quargnento (AL) - Tel 0131219925 - Fax 0131219926 –
e-mail: [email protected] – web: www.satasrl.it
1 GEP COMPLIANCE STATEMENT
The field trial was performed in according with Sponsor study protocol.
The trial was managed according to the OEPP/EPPO Guidelines on Efficacy Evaluation of
Plant Protection Product:
PP 1/166(3) Erwinia amylovora
PP 1/152(4) Design and analysis of efficacy evaluation trials
PP 1/135(3) Phytotoxicity assessment
PP 1/181(4) Conduct and reporting of efficacy evaluation trials including GEP
L.D. March 1995 n.194, accomplishment of Directive 91/414/ECC and Regulation N.
1107/2009.
Internal SOPs.
Study Director: Alessandro Costanzo Date: 28/11/2019
Signature:
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PROTOCOL ID: 1062018G_B SATA TRIAL ID: 1062018G_B_01
Efficacy and selectivity of Anolyte (AcquAttiva®) to control Erwinia amylovora causing fireblight on
BIO pear
Pag. 4 di 38
Pagina 4 di 38
SATA S.r.l. – Strada Alessandria, 13 – 15044 Quargnento (AL) - Tel 0131219925 - Fax 0131219926 –
e-mail: [email protected] – web: www.satasrl.it
2 GEP OFFICIAL ACCREDITATION
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PROTOCOL ID: 1062018G_B SATA TRIAL ID: 1062018G_B_01
Efficacy and selectivity of Anolyte (AcquAttiva®) to control Erwinia amylovora causing fireblight on
BIO pear
Pag. 5 di 38
Pagina 5 di 38
SATA S.r.l. – Strada Alessandria, 13 – 15044 Quargnento (AL) - Tel 0131219925 - Fax 0131219926 –
e-mail: [email protected] – web: www.satasrl.it
Innovare e crescere insieme
PROTOCOL ID: 1062018G_B SATA TRIAL ID: 1062018G_B_01
Efficacy and selectivity of Anolyte (AcquAttiva®) to control Erwinia amylovora causing fireblight on
BIO pear
Pag. 6 di 38
Pagina 6 di 38
SATA S.r.l. – Strada Alessandria, 13 – 15044 Quargnento (AL) - Tel 0131219925 - Fax 0131219926 –
e-mail: [email protected] – web: www.satasrl.it
3 ARCHIVING STATEMENT
The protocol, raw data and copy of the final report will be archived for ten years in the office GEP
of SATA srl, Strada Alessandria, 13 – 15044 Quargnento (AL) – Italy.
4 SUMMARY
The experimental field trial was carried out in the countryside of Centallo (province of Cuneo –
Northern Italy) from May 2019 to July 2019, in order to evaluate the efficacy and selectivity of
Anolyte (AcquAttiva®) applied on BIO pear to control Erwinia amylovora (ERWIAM).
Anolyte (AcquAttiva®) was been tested in comparison to organic standard control strategy (Amylo X+Vacciplant+Poltiglia Disperss). Disease symptoms were visible at 30/05/2019 but the disease pressure was very low and concentrated on the buds. Anolyte (AcquAttiva®) showed good efficacy to control disease, but efficacy evaluation was not completely verified because the disease pressure was very low. All treatments did not show any phytotoxicity symptoms on the crop. La prova sperimentale è stata condotta a Centallo (provincia di Cuneo – Nord Italia) da Maggio 2019 a Luglio 2019, al fine di valutare l’efficacia e la selettività di Anolyte (AcquAttiva®) per il controllo di Erwinia amylovora (ERWIAM). Anolyte (AcquAttiva®) è stata testata a confronto con una strategia BIO standard (Amylo X+Vacciplant+Poltiglia Disperss). I sintomi della malattia erano visibili al 30/05/2019, ma la pressione era molto bassa e concentrata sui germogli. Anolyte (AcquAttiva®) ha mostrato una buona efficacia nel controllo della malattia, ma la valutazione dell’efficacia non è completamente verificata in quanto la pressione della malattia era molto bassa. Tutti i trattamenti non hanno mostrato alcun sintomo di fitotossicità sulla coltura.
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PROTOCOL ID: 1062018G_B SATA TRIAL ID: 1062018G_B_01
Efficacy and selectivity of Anolyte (AcquAttiva®) to control Erwinia amylovora causing fireblight on
BIO pear
Pag. 7 di 38
Pagina 7 di 38
SATA S.r.l. – Strada Alessandria, 13 – 15044 Quargnento (AL) - Tel 0131219925 - Fax 0131219926 –
e-mail: [email protected] – web: www.satasrl.it
5 TRIAL INFORMATION & METHODS
5.1 TRIAL TREATMENTS Trt Treatment Form Form Form Specific Rate Appl No. Type Name Conc Unit Type Gravity Description Supplier Rate Unit Code
1 CHK Untreated Check not treated
2 PLHE Anolyte - AcquaAttiva L 450 l/ha ABCDEFGH 3 FUNG Amylo-X 250 gA/kg WG 1 CBC 2 kg/ha ABDF -Bacillus amyloliquefacens 250 500 g AI FUNG Vacciplant 45 gA/L SL 1,05 Ary 1 l/ha CE -Laminarina 45 45 g AI FUNG Poltiglia Disperss 200 gA/kg WG 1 UPL 5 kg/ha GH -Rame metallo 200 1000 g AI
Replications: 4, Untreated treatments: 1, Conduct under GLP/GEP: Yes (GEP with no protection), Design: Randomized Complete Block (RCB), Treatment units: Treated 'Plot' experimental unit size, Dry Form. Unit: %, Treated 'Plot' experimental unit size Width: 4 meters, Treated 'Plot' experimental unit size Length: 9 meters, Application amount: 900 L/ha, Mix size: 14.96 L, Overage: 2000 mL, Format definitions: G-All7.def, G-All7.frm
5.2 GENERAL TRIAL INFORMATION
Study Director: Alessandro Costanzo Title: Dott. Investigator: Ivano RAMON Title: Dott.
Discipline: F fungicide
Trial Status: E established ARM Trial Created On: Mar-25-2019
Initiation Date: Mar-27-2019 Completion Date: Jul-26-2019
Conducted Under GLP: No Conducted Under GEP: Yes
No. Guideline Discipline Description
1. PP 1/181(4) GS Conduct and reporting of efficacy evaluation trials including GEP
2. PP 1/152(4) GS Design and analysis of efficacy evaluation trials 3. PP 1/135(4) GS Phytotoxicity assessment
4. PP 1/166(3) F Erwinia amylovora
Objectives: To evaluate efficacy and selectivity of Anolyte (AcquAttiva®) to control Erwinia amylovora causing fireblight on BIO pear
5.3 TRIAL LOCATION Trial Location
City: Centallo Country: ITA Italy State/Prov.: Cuneo
Postal Code: 12044 Climate Zone: EPOMED EPPO Mediterranean
Latitude of LL Corner °: 44,5081 N
Longitude of LL Corner °: 7,5956 E Altitude of LL Corner: 424,00 m
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Efficacy and selectivity of Anolyte (AcquAttiva®) to control Erwinia amylovora causing fireblight on
BIO pear
Pag. 8 di 38
Pagina 8 di 38
SATA S.r.l. – Strada Alessandria, 13 – 15044 Quargnento (AL) - Tel 0131219925 - Fax 0131219926 –
e-mail: [email protected] – web: www.satasrl.it
5.4 CONTACTS
Study Director: Alessandro Costanzo Title: Dott. Organization: SATA srl
Address: Strada Alessandria 13 Phone No.: +39 0131 219925 City+State/Prov: Quargnento Mobile No.: +39 349 2559462
Postal Code: 15044 E-mail: [email protected] Country: Ita
Investigator: Ivano RAMON Title: Dott.
Organization: SATA srl Address: Strada Alessandria 13 Phone No.: +39 0131 219925
City+State/Prov: Quargnento Mobile No.: +39 346 3906013 Postal Code: 15044 E-mail: [email protected]
Country: Ita Other Contacts
Name Role Other
Marco Capra Tecnician SATA srl
Massimiliano Amerelli Tecnician SATA srl
5.5 COOPERATOR/LANDOWNER Cooperator/Landowner
Cooperator: Società agricola Aia Piccola Role: FALDOW Address 1: Reg. Crosia, 365
City: Centallo State/Prov: CN
Postal Code: 12044 Country: Ita
5.6 CROP DESCRIPTION
Crop 1: C PYUCO Pyrus communis Common pear BBCH Scale: BPOM Stage Scale: BBCH
Variety: Abate Perennial Age: 7 YR
Planting Density: 1250 P/ha Row Spacing: 4 m
Spacing within Row: 2 m
5.7 PEST DESCRIPTION
Pest 1 Type: D Code: ERWIAM Erwinia amylovora Common Name: Fire blight Entry Date: Sep-10-2019
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Efficacy and selectivity of Anolyte (AcquAttiva®) to control Erwinia amylovora causing fireblight on
BIO pear
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e-mail: [email protected] – web: www.satasrl.it
5.8 SITE AND DESIGN
Treated Plot Width: 4 m Treated Plot Length: 9 m
Treated Plot Area: 36 m2 Treatments: 3 Replications: 4 Study Design: RACOBL Randomized Complete Block (RCB)
5.9 FIELD PLOT LAYOUT
403 1
401 2
402 3
301 1
303 3
302 2
201 3
202 1
203 2
102 3
101 2
103 1
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Efficacy and selectivity of Anolyte (AcquAttiva®) to control Erwinia amylovora causing fireblight on
BIO pear
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e-mail: [email protected] – web: www.satasrl.it
5.10 APPLICATION DESCRIPTION
A B C D E F G
Application Date Mar-27-2019 Apr-2-2019 Apr-9-2019 Apr-17-2019 Apr-25-2019 May-3-2019 May-22-2019 Appl. Start Time 11:00 11:50 11:20 11:15 10:45 14:20 11:50
Appl. Stop Time 11:30 12:20 12:17 11:40 11:00 14:50 12:20
Application Method SPRAY SPRAY SPRAY SPRAY SPRAY SPRAY SPRAY
Application Timing BBCH 59-60 BBCH 67-69 DAA 7-10 DAA 7-10 DAA 7-10 DAA 7-10 Application Placement FOLIAR FOLIAR FOLIAR FOLIAR FOLIAR FOLIAR FOLIAR
Applied By M. Capra M. Capra M. Capra M. Capra M. Capra M. Capra M. Capra
Appl. Entry Date Sep-10-2019 Sep-10-2019 Sep-10-2019 Sep-10-2019 Sep-10-2019 Sep-10-2019 Sep-10-2019
Air Temperature Start, Stop 13,9 14,5 C 21,8 22,0 C 16,9 15,7 C 23,5 24,8 C 13,2 13,4 C 21,2 21,3 C 23,4 24,8 C % Relative Humidity Start, Stop 41,1 35,2 42,4 41,9 61,6 70,2 44,7 42,8 70,2 71,2 58,2 59,2 59,2 59
Wind Velocity+Dir. Start 0 KPH 0 KPH 2,6 KPH SE 0 KPH 0 KPH 5,6 KPH SW 0 KPH
Wind Velocity+Dir. Stop 0 KPH 0 KPH 3,2 KPH SE 0 KPH 0 KPH 4,8 KPH SW 0 KPH
Wind Velocity+Dir. Max 0 KPH 0 KPH 3,2 KPH SE 0 KPH 0 KPH 5,6 KPH SW 0 KPH Wet Leaves (Y/N) N no N no N no N no N no N no N no
Soil Moisture DRY DRY WET WET WET NORMAL NORMAL
% Cloud Cover 0 20 0 0 80 90 10
H
Application Date May-30-2019
Appl. Start Time 11:25
Appl. Stop Time 12:00 Application Method SPRAY
Application Timing
Application Placement FOLIAR
Applied By M. Capra Appl. Entry Date Sep-10-2019
Air Temperature Start, Stop 24,9 25,0 C
% Relative Humidity Start, Stop 39,7 38,6 Wind Velocity+Dir. Start 3,1 KPH E
Wind Velocity+Dir. Stop 6,3 KPH E
Wind Velocity+Dir. Max 6,3 KPH E
Wet Leaves (Y/N) N no Soil Moisture NORMAL
% Cloud Cover 0
5.11 CROP STAGE AT EACH APPLICATION
A B C D E F Crop 1 Code, BBCH Scale PYUCO BPOM PYUCO BPOM PYUCO BPOM PYUCO BPOM PYUCO BPOM PYUCO BPOM
Stage Scale Used BBCH BBCH BBCH BBCH BBCH BBCH
Stage Majority, Percent 59 65 65 69 71 72
Stage Minimum, Percent 59 65 65 69 71 72 Stage Maximum, Percent 60 65 67 69 71 72
G H Crop 1 Code, BBCH Scale PYUCO BPOM PYUCO BPOM
Stage Scale Used BBCH BBCH
Stage Majority, Percent 73 74
Stage Minimum, Percent 73 74 Stage Maximum, Percent 73 74
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Efficacy and selectivity of Anolyte (AcquAttiva®) to control Erwinia amylovora causing fireblight on
BIO pear
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Pagina 11 di 38
SATA S.r.l. – Strada Alessandria, 13 – 15044 Quargnento (AL) - Tel 0131219925 - Fax 0131219926 –
e-mail: [email protected] – web: www.satasrl.it
5.12 PEST STAGE AT EACH APPLICATION
A B C D E F G H
Pest 1 Code, Type, Scale ERWIAM D ERWIAM D ERWIAM D ERWIAM D ERWIAM D ERWIAM D ERWIAM D ERWIAM D Stage Majority, Percent PRINFC PRINFC PRINFC PRINFC PRINFC PRINFC PRINFC PRINFC
Stage Minimum, Percent PRINFC PRINFC PRINFC PRINFC PRINFC PRINFC PRINFC PRINFC
Stage Maximum, Percent PRINFC PRINFC PRINFC PRINFC PRINFC PRINFC PRINFC PRINFC
5.13 APPLICATION EQUIPMENT
A B C D E F G H
Equipment Type KNAMOT KNAMOT KNAMOT KNAMOT KNAMOT KNAMOT KNAMOT KNAMOT
Operation Pressure 10 BAR 10 BAR 10 BAR 10 BAR 10 BAR 10 BAR 10 BAR 10 BAR
Nozzle Type CONSOL CONSOL CONSOL CONSOL CONSOL CONSOL CONSOL CONSOL Nozzle Size 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5
Boom ID SataNord082 SataNord082 SataNord082 SataNord082 SataNord082 SataNord082 SataNord082 SataNord082
Carrier WATER WATER WATER WATER WATER WATER WATER WATER
Application Amount 900 L/ha 900 L/ha 900 L/ha 900 L/ha 900 L/ha 900 L/ha 900 L/ha 900 L/ha Mix Overage 2000 mL 2000 mL 2000 mL 2000 mL 2000 mL 2000 mL 2000 mL 2000 mL
Mix Size 14,96 L 14,96 L 14,96 L 14,96 L 14,96 L 14,96 L 14,96 L 14,96 L
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Efficacy and selectivity of Anolyte (AcquAttiva®) to control Erwinia amylovora causing fireblight on
BIO pear
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Pagina 12 di 38
SATA S.r.l. – Strada Alessandria, 13 – 15044 Quargnento (AL) - Tel 0131219925 - Fax 0131219926 –
e-mail: [email protected] – web: www.satasrl.it
6 MOISTURE AND WEATHER CONDITIONS
Closest Weather Station: Centallo Distance: 5 km
Chart 1 - Temperatures and rainfall during trial
(Source: Rete Agrometeorologica del Piemonte - Regione Piemonte - Assessorato Agricoltura - Settore Fitosanitario - Sezione di Agrometeorologia)
7 STATISTICAL ANALYSIS METHODS
Data collected during the trial were subjected to analysis of variance ANOVA. Treatment average
means were calculated and were compared using the TUKEY HSD test (p=0.05). Statistical
procedures were applied using the ARM 2019 from Gylling Data Management.
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Efficacy and selectivity of Anolyte (AcquAttiva®) to control Erwinia amylovora causing fireblight on
BIO pear
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Pagina 13 di 38
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e-mail: [email protected] – web: www.satasrl.it
8 RESULTS
8.1 EFFICACY Efficacy evaluation was carried out counting the number of buds infected by ERWIAM. The buds infected was been removed on each plots. At first assessment Anolyte (AcquAttiva®) showed good efficacy to control disease, but Bio standard strategy showed higher efficacy (Chart 2, paragraph 10.3). The second assessment was carried out 14 days after first assessment and Anolyte (AcquAttiva®) showed good efficacy to control disease (Chart 3, paragraph 10.3). Successively, all assessments have not showed any deasease symptoms. La valutazione dell’efficacia è stata effettata contando il numero di germogli infetti da ERWIAM. I germogli infetti sono stati rimossi da ogni plots. Alla prima valutazione Anolyte (AcquAttiva®) mostrava una buona efficacia nel controllo della malattia, ma la strategia Bio di riferimento ha mostrato un’efficacia più elevata (grafico 2, paragrafo 10.3). Alla seconda valutazione effettuata 14 giorni dopo la prima, Anolyte (AcquAttiva®) ha mostrato nuovamente una buona efficacia nel contenimento della malattia (grafico 3, paragrafo 10.3). Successivamente, in tutte le valutazioni non sis ono riscontrati sintomi legati a ERWIAM.
8.2 PHYTOTOXICITY All assessments have not showed any phytotoxicity symptoms. Tutte le valutazionI non hanno mostrato alcun sintomo di fitotossicità.
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Efficacy and selectivity of Anolyte (AcquAttiva®) to control Erwinia amylovora causing fireblight on
BIO pear
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SATA S.r.l. – Strada Alessandria, 13 – 15044 Quargnento (AL) - Tel 0131219925 - Fax 0131219926 –
e-mail: [email protected] – web: www.satasrl.it
9 FINAL REMARKS
The experimental field trial was carried out in the countryside of Centallo (province of Cuneo –
Northern Italy) from May 2019 to July 2019, in order to evaluate the efficacy and selectivity of
Anolyte (AcquAttiva®) applied on BIO pear to control Erwinia amylovora (ERWIAM).
Anolyte (AcquAttiva®) showed good efficacy to control disease, but efficacy evaluation was not completely verified because the disease pressure was very low. All treatments did not show any phytotoxicity symptoms on the crop. La prova sperimentale è stata condotta a Centallo (provincia di Cuneo – Nord Italia) da Maggio 2019 a Luglio 2019, al fine di valutare l’efficacia e la selettività di Anolyte (AcquAttiva®) per il controllo di Erwinia amylovora (ERWIAM). Anolyte (AcquAttiva®) ha mostrato una buona efficacia nel controllo della malattia, ma la valutazione dell’efficacia non è stata completamente verificata in quanto la pressione della malattia era molto bassa. Tutti i trattamenti non hanno mostrato alcun sintomo di fitotossicità sulla coltura.
Innovare e crescere insieme
PROTOCOL ID: 1062018G_B SATA TRIAL ID: 1062018G_B_01
Efficacy and selectivity of Anolyte (AcquAttiva®) to control Erwinia amylovora causing fireblight on
BIO pear
Pag. 15 di 38
Pagina 15 di 38
SATA S.r.l. – Strada Alessandria, 13 – 15044 Quargnento (AL) - Tel 0131219925 - Fax 0131219926 –
e-mail: [email protected] – web: www.satasrl.it
10 ANNEX
10.1 RAW DATA Pest Type D Disease D Disease D Disease D Disease Pest Code ERWIAM ERWIAM ERWIAM ERWIAM Pest Scientific Name Erwinia amylov> Erwinia amylov> Erwinia amylov> Erwinia amylov> Pest Name Fire blight Fire blight Fire blight Fire blight Crop Type, Code C PYUCO C PYUCO C PYUCO C PYUCO BBCH Scale BPOM BPOM BPOM BPOM Crop Scientific Name Pyrus communis Pyrus communis Pyrus communis Pyrus communis Crop Name Common pear Common pear Common pear Common pear Rating Date Mar-27-2019 Mar-27-2019 Apr-2-2019 Apr-2-2019 SE Group No. 22 1 23 2 Part Rated BUD P FLOCLU P BUD P FLOCLU P Rating Type COUINF COUINF COUINF COUINF Rating Unit NUMBER NUMBER NUMBER NUMBER Sample Size 1 PLOT 1 PLOT 1 PLOT 1 PLOT Number of Subsamples 1 1 1 1 Crop Stage Scale BBCH BBCH BBCH BBCH Crop Stage Majority 59 59 65 65 Crop Stage Minimum/Maximum 60 60 60 60 65 65 65 65 Assessed By I. Ramon I. Ramon I. Ramon I. Ramon Data Entry Date Sep-10-2019 Sep-10-2019 Sep-10-2019 Sep-10-2019 Days After First/Last Applic. 0 0 0 0 6 6 6 6
Trt Treatment Rate Appl No. Name Rate Unit Code Plot 1 2 3 4
1 Untreated Check 103 0,0 0,0 0,0 0,0 202 0,0 0,0 0,0 0,0 301 0,0 0,0 0,0 0,0 403 0,0 0,0 0,0 0,0
Mean = 0,0 0,0 0,0 0,0
2 Anolyte - AcquaAttiva 450 l/ha ABCDEFGH 101 0,0 0,0 0,0 0,0 203 0,0 0,0 0,0 0,0 302 0,0 0,0 0,0 0,0 401 0,0 0,0 0,0 0,0
Mean = 0,0 0,0 0,0 0,0
3 Amylo-X 2 kg/ha ABDF 102 0,0 0,0 0,0 0,0 Vacciplant 1 l/ha CE 201 0,0 0,0 0,0 0,0 Poltiglia Disperss 5 kg/ha GH 303 0,0 0,0 0,0 0,0
402 0,0 0,0 0,0 0,0 Mean = 0,0 0,0 0,0 0,0
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Efficacy and selectivity of Anolyte (AcquAttiva®) to control Erwinia amylovora causing fireblight on
BIO pear
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Pest Type D Disease D Disease Pest Code ERWIAM ERWIAM Pest Scientific Name Erwinia amylov> Erwinia amylov> Pest Name Fire blight Fire blight Crop Type, Code C PYUCO C PYUCO C PYUCO C PYUCO BBCH Scale BPOM BPOM BPOM BPOM Crop Scientific Name Pyrus communis Pyrus communis Pyrus communis Pyrus communis Crop Name Common pear Common pear Common pear Common pear Rating Date Apr-2-2019 Apr-9-2019 Apr-9-2019 Apr-9-2019 SE Group No. 15 24 3 16 Part Rated PLANT C BUD P FLOCLU P PLANT C Rating Type PHYGEN COUINF COUINF PHYGEN Rating Unit % NUMBER NUMBER % Sample Size 1 PLOT 1 PLOT 1 PLOT 1 PLOT Number of Subsamples 1 1 1 1 Crop Stage Scale BBCH BBCH BBCH BBCH Crop Stage Majority 65 65 65 65 Crop Stage Minimum/Maximum 65 65 65 67 65 67 65 67 Assessed By I. Ramon I. Ramon I. Ramon I. Ramon Data Entry Date Sep-10-2019 Days After First/Last Applic. 6 6 13 7 13 7 13 7
Trt Treatment Rate Appl No. Name Rate Unit Code Plot 5 6 7 8
1 Untreated Check 103 0,0 0,0 0,0 0,0 202 0,0 0,0 0,0 0,0 301 0,0 0,0 0,0 0,0 403 0,0 0,0 0,0 0,0
Mean = 0,0 0,0 0,0 0,0
2 Anolyte - AcquaAttiva 450 l/ha ABCDEFGH 101 0,0 0,0 0,0 0,0 203 0,0 0,0 0,0 0,0 302 0,0 0,0 0,0 0,0 401 0,0 0,0 0,0 0,0
Mean = 0,0 0,0 0,0 0,0 3 Amylo-X 2 kg/ha ABDF 102 0,0 0,0 0,0 0,0 Vacciplant 1 l/ha CE 201 0,0 0,0 0,0 0,0 Poltiglia Disperss 5 kg/ha GH 303 0,0 0,0 0,0 0,0
402 0,0 0,0 0,0 0,0 Mean = 0,0 0,0 0,0 0,0
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Efficacy and selectivity of Anolyte (AcquAttiva®) to control Erwinia amylovora causing fireblight on
BIO pear
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Pagina 17 di 38
SATA S.r.l. – Strada Alessandria, 13 – 15044 Quargnento (AL) - Tel 0131219925 - Fax 0131219926 –
e-mail: [email protected] – web: www.satasrl.it
Pest Type D Disease D Disease D Disease Pest Code ERWIAM ERWIAM ERWIAM Pest Scientific Name Erwinia amylov> Erwinia amylov> Erwinia amylov> Pest Name Fire blight Fire blight Fire blight Crop Type, Code C PYUCO C PYUCO C PYUCO C PYUCO BBCH Scale BPOM BPOM BPOM BPOM Crop Scientific Name Pyrus communis Pyrus communis Pyrus communis Pyrus communis Crop Name Common pear Common pear Common pear Common pear Rating Date Apr-17-2019 Apr-17-2019 Apr-17-2019 Apr-25-2019 SE Group No. 25 4 17 26 Part Rated BUD P FRUATT P PLANT C BUD P Rating Type COUINF COUINF PHYGEN COUINF Rating Unit NUMBER NUMBER % NUMBER Sample Size 1 PLOT 1 PLOT 1 PLOT 1 PLOT Number of Subsamples 1 1 1 1 Crop Stage Scale BBCH BBCH BBCH BBCH Crop Stage Majority 69 69 69 71 Crop Stage Minimum/Maximum 69 69 69 69 69 69 71 71 Assessed By I. Ramon I. Ramon I. Ramon I. Ramon Data Entry Date Sep-10-2019 Sep-10-2019 Days After First/Last Applic. 21 8 21 8 21 8 29 8
Trt Treatment Rate Appl No. Name Rate Unit Code Plot 9 10 11 12
1 Untreated Check 103 0,0 0,0 0,0 0,0 202 0,0 0,0 0,0 0,0 301 0,0 0,0 0,0 0,0 403 0,0 0,0 0,0 0,0
Mean = 0,0 0,0 0,0 0,0
2 Anolyte - AcquaAttiva 450 l/ha ABCDEFGH 101 0,0 0,0 0,0 0,0 203 0,0 0,0 0,0 0,0 302 0,0 0,0 0,0 0,0 401 0,0 0,0 0,0 0,0
Mean = 0,0 0,0 0,0 0,0 3 Amylo-X 2 kg/ha ABDF 102 0,0 0,0 0,0 0,0 Vacciplant 1 l/ha CE 201 0,0 0,0 0,0 0,0 Poltiglia Disperss 5 kg/ha GH 303 0,0 0,0 0,0 0,0
402 0,0 0,0 0,0 0,0 Mean = 0,0 0,0 0,0 0,0
Innovare e crescere insieme
PROTOCOL ID: 1062018G_B SATA TRIAL ID: 1062018G_B_01
Efficacy and selectivity of Anolyte (AcquAttiva®) to control Erwinia amylovora causing fireblight on
BIO pear
Pag. 18 di 38
Pagina 18 di 38
SATA S.r.l. – Strada Alessandria, 13 – 15044 Quargnento (AL) - Tel 0131219925 - Fax 0131219926 –
e-mail: [email protected] – web: www.satasrl.it
Pest Type D Disease D Disease D Disease Pest Code ERWIAM ERWIAM ERWIAM Pest Scientific Name Erwinia amylov> Erwinia amylov> Erwinia amylov> Pest Name Fire blight Fire blight Fire blight Crop Type, Code C PYUCO C PYUCO C PYUCO C PYUCO BBCH Scale BPOM BPOM BPOM BPOM Crop Scientific Name Pyrus communis Pyrus communis Pyrus communis Pyrus communis Crop Name Common pear Common pear Common pear Common pear Rating Date Apr-25-2019 Apr-25-2019 May-3-2019 May-3-2019 SE Group No. 5 18 27 6 Part Rated FRUATT P PLANT C BUD P FRUATT P Rating Type COUINF PHYGEN COUINF COUINF Rating Unit NUMBER % NUMBER NUMBER Sample Size 1 PLOT 1 PLOT 1 PLOT 1 PLOT Number of Subsamples 1 1 1 1 Crop Stage Scale BBCH BBCH BBCH BBCH Crop Stage Majority 71 71 72 72 Crop Stage Minimum/Maximum 71 71 71 71 72 72 72 72 Assessed By I. Ramon I. Ramon I. Ramon I. Ramon Data Entry Date Sep-10-2019 Days After First/Last Applic. 29 8 29 8 37 8 37 8
Trt Treatment Rate Appl No. Name Rate Unit Code Plot 13 14 15 16
1 Untreated Check 103 0,0 0,0 0,0 0,0 202 0,0 0,0 0,0 0,0 301 0,0 0,0 0,0 0,0 403 0,0 0,0 0,0 0,0
Mean = 0,0 0,0 0,0 0,0
2 Anolyte - AcquaAttiva 450 l/ha ABCDEFGH 101 0,0 0,0 0,0 0,0 203 0,0 0,0 0,0 0,0 302 0,0 0,0 0,0 0,0 401 0,0 0,0 0,0 0,0
Mean = 0,0 0,0 0,0 0,0 3 Amylo-X 2 kg/ha ABDF 102 0,0 0,0 0,0 0,0 Vacciplant 1 l/ha CE 201 0,0 0,0 0,0 0,0 Poltiglia Disperss 5 kg/ha GH 303 0,0 0,0 0,0 0,0
402 0,0 0,0 0,0 0,0 Mean = 0,0 0,0 0,0 0,0
Innovare e crescere insieme
PROTOCOL ID: 1062018G_B SATA TRIAL ID: 1062018G_B_01
Efficacy and selectivity of Anolyte (AcquAttiva®) to control Erwinia amylovora causing fireblight on
BIO pear
Pag. 19 di 38
Pagina 19 di 38
SATA S.r.l. – Strada Alessandria, 13 – 15044 Quargnento (AL) - Tel 0131219925 - Fax 0131219926 –
e-mail: [email protected] – web: www.satasrl.it
Pest Type D Disease D Disease Pest Code ERWIAM ERWIAM Pest Scientific Name Erwinia amylov> Erwinia amylov> Pest Name Fire blight Fire blight Crop Type, Code C PYUCO C PYUCO C PYUCO C PYUCO BBCH Scale BPOM BPOM BPOM BPOM Crop Scientific Name Pyrus communis Pyrus communis Pyrus communis Pyrus communis Crop Name Common pear Common pear Common pear Common pear Rating Date May-3-2019 May-22-2019 May-22-2019 May-22-2019 SE Group No. 19 28 7 20 Part Rated PLANT C BUD P FRUATT P FRUATT P Rating Type PHYGEN COUINF COUINF COUINF Rating Unit % NUMBER NUMBER NUMBER Sample Size 1 PLOT 1 PLOT 1 PLOT 1 PLOT Number of Subsamples 1 1 1 1 Crop Stage Scale BBCH BBCH BBCH BBCH Crop Stage Majority 72 73 73 73 Crop Stage Minimum/Maximum 72 72 73 73 73 73 73 73 Assessed By I. Ramon I. Ramon I. Ramon I. Ramon Data Entry Date Sep-10-2019 Sep-10-2019 Days After First/Last Applic. 37 8 56 19 56 19 56 19
Trt Treatment Rate Appl No. Name Rate Unit Code Plot 17 18 19 20
1 Untreated Check 103 0,0 0,0 0,0 0,0 202 0,0 0,0 0,0 0,0 301 0,0 0,0 0,0 0,0 403 0,0 0,0 0,0 0,0
Mean = 0,0 0,0 0,0 0,0
2 Anolyte - AcquaAttiva 450 l/ha ABCDEFGH 101 0,0 0,0 0,0 0,0 203 0,0 0,0 0,0 0,0 302 0,0 0,0 0,0 0,0 401 0,0 0,0 0,0 0,0
Mean = 0,0 0,0 0,0 0,0 3 Amylo-X 2 kg/ha ABDF 102 0,0 0,0 0,0 0,0 Vacciplant 1 l/ha CE 201 0,0 0,0 0,0 0,0 Poltiglia Disperss 5 kg/ha GH 303 0,0 0,0 0,0 0,0
402 0,0 0,0 0,0 0,0 Mean = 0,0 0,0 0,0 0,0
Innovare e crescere insieme
PROTOCOL ID: 1062018G_B SATA TRIAL ID: 1062018G_B_01
Efficacy and selectivity of Anolyte (AcquAttiva®) to control Erwinia amylovora causing fireblight on
BIO pear
Pag. 20 di 38
Pagina 20 di 38
SATA S.r.l. – Strada Alessandria, 13 – 15044 Quargnento (AL) - Tel 0131219925 - Fax 0131219926 –
e-mail: [email protected] – web: www.satasrl.it
Pest Type D Disease D Disease D Disease Pest Code ERWIAM ERWIAM ERWIAM Pest Scientific Name Erwinia amylov> Erwinia amylov> Erwinia amylov> Pest Name Fire blight Fire blight Fire blight Crop Type, Code C PYUCO C PYUCO C PYUCO C PYUCO BBCH Scale BPOM BPOM BPOM BPOM Crop Scientific Name Pyrus communis Pyrus communis Pyrus communis Pyrus communis Crop Name Common pear Common pear Common pear Common pear Rating Date May-30-2019 May-30-2019 May-30-2019 Jun-7-2019 SE Group No. 29 8 21 30 Part Rated BUD P FRUATT P BUD P BUD P Rating Type COUINF COUINF COUINF COUINF Rating Unit NUMBER NUMBER NUMBER NUMBER Sample Size 1 PLOT 1 PLOT 1 PLOT 1 PLOT Number of Subsamples 1 1 1 1 Crop Stage Scale BBCH BBCH BBCH BBCH Crop Stage Majority 74 74 74 75 Crop Stage Minimum/Maximum 74 74 74 74 74 74 75 75 Assessed By I. Ramon I. Ramon I. Ramon I. Ramon Data Entry Date Sep-10-2019 Sep-10-2019 Sep-10-2019 Sep-10-2019 Days After First/Last Applic. 64 8 64 8 64 8 72 8
Trt Treatment Rate Appl No. Name Rate Unit Code Plot 21 22 23 24
1 Untreated Check 103 20,0 0,0 0,0 0,0 202 6,0 0,0 0,0 0,0 301 10,0 0,0 0,0 0,0 403 13,0 0,0 0,0 0,0
Mean = 12,3 0,0 0,0 0,0
2 Anolyte - AcquaAttiva 450 l/ha ABCDEFGH 101 9,0 0,0 0,0 0,0 203 6,0 0,0 0,0 0,0 302 4,0 0,0 0,0 0,0 401 7,0 0,0 0,0 0,0
Mean = 6,5 0,0 0,0 0,0 3 Amylo-X 2 kg/ha ABDF 102 8,0 0,0 0,0 0,0 Vacciplant 1 l/ha CE 201 5,0 0,0 0,0 0,0 Poltiglia Disperss 5 kg/ha GH 303 2,0 0,0 0,0 0,0
402 5,0 0,0 0,0 0,0 Mean = 5,0 0,0 0,0 0,0
Innovare e crescere insieme
PROTOCOL ID: 1062018G_B SATA TRIAL ID: 1062018G_B_01
Efficacy and selectivity of Anolyte (AcquAttiva®) to control Erwinia amylovora causing fireblight on
BIO pear
Pag. 21 di 38
Pagina 21 di 38
SATA S.r.l. – Strada Alessandria, 13 – 15044 Quargnento (AL) - Tel 0131219925 - Fax 0131219926 –
e-mail: [email protected] – web: www.satasrl.it
Pest Type D Disease D Disease D Disease D Disease Pest Code ERWIAM ERWIAM ERWIAM ERWIAM Pest Scientific Name Erwinia amylov> Erwinia amylov> Erwinia amylov> Erwinia amylov> Pest Name Fire blight Fire blight Fire blight Fire blight Crop Type, Code C PYUCO C PYUCO C PYUCO C PYUCO BBCH Scale BPOM BPOM BPOM BPOM Crop Scientific Name Pyrus communis Pyrus communis Pyrus communis Pyrus communis Crop Name Common pear Common pear Common pear Common pear Rating Date Jun-7-2019 Jun-14-2019 Jun-14-2019 Jun-21-2019 SE Group No. 9 31 10 32 Part Rated FRUATT P BUD P FRUATT P BUD P Rating Type COUINF COUINF COUINF COUINF Rating Unit NUMBER NUMBER NUMBER NUMBER Sample Size 1 PLOT 1 PLOT 1 PLOT 1 PLOT Number of Subsamples 1 1 1 1 Crop Stage Scale BBCH BBCH BBCH BBCH Crop Stage Majority 75 76 76 77 Crop Stage Minimum/Maximum 75 75 76 76 76 76 77 77 Assessed By I. Ramon I. Ramon I. Ramon I. Ramon Data Entry Date Sep-10-2019 Nov-28-2019 Sep-10-2019 Sep-10-2019 Days After First/Last Applic. 72 8 79 15 79 15 86 22
Trt Treatment Rate Appl No. Name Rate Unit Code Plot 25 26 27 28
1 Untreated Check 103 0,0 17,0 0,0 0,0 202 0,0 16,0 0,0 0,0 301 0,0 19,0 0,0 0,0 403 0,0 15,0 0,0 0,0
Mean = 0,0 16,8 0,0 0,0
2 Anolyte - AcquaAttiva 450 l/ha ABCDEFGH 101 0,0 13,0 0,0 0,0 203 0,0 11,0 0,0 0,0 302 0,0 9,0 0,0 0,0 401 0,0 11,0 0,0 0,0
Mean = 0,0 11,0 0,0 0,0 3 Amylo-X 2 kg/ha ABDF 102 0,0 5,0 0,0 0,0 Vacciplant 1 l/ha CE 201 0,0 5,0 0,0 0,0 Poltiglia Disperss 5 kg/ha GH 303 0,0 1,0 0,0 0,0
402 0,0 6,0 0,0 0,0 Mean = 0,0 4,3 0,0 0,0
Innovare e crescere insieme
PROTOCOL ID: 1062018G_B SATA TRIAL ID: 1062018G_B_01
Efficacy and selectivity of Anolyte (AcquAttiva®) to control Erwinia amylovora causing fireblight on
BIO pear
Pag. 22 di 38
Pagina 22 di 38
SATA S.r.l. – Strada Alessandria, 13 – 15044 Quargnento (AL) - Tel 0131219925 - Fax 0131219926 –
e-mail: [email protected] – web: www.satasrl.it
Pest Type D Disease D Disease D Disease D Disease Pest Code ERWIAM ERWIAM ERWIAM ERWIAM Pest Scientific Name Erwinia amylov> Erwinia amylov> Erwinia amylov> Erwinia amylov> Pest Name Fire blight Fire blight Fire blight Fire blight Crop Type, Code C PYUCO C PYUCO C PYUCO C PYUCO BBCH Scale BPOM BPOM BPOM BPOM Crop Scientific Name Pyrus communis Pyrus communis Pyrus communis Pyrus communis Crop Name Common pear Common pear Common pear Common pear Rating Date Jun-21-2019 Jun-28-2019 Jun-28-2019 Jul-12-2019 SE Group No. 11 33 12 34 Part Rated FRUATT P BUD P FRUATT P BUD P Rating Type COUINF COUINF COUINF COUINF Rating Unit NUMBER NUMBER NUMBER NUMBER Sample Size 1 PLOT 1 PLOT 1 PLOT 1 PLOT Number of Subsamples 1 1 1 1 Crop Stage Scale BBCH BBCH BBCH BBCH Crop Stage Majority 77 78 78 78 Crop Stage Minimum/Maximum 77 77 78 78 78 78 78 78 Assessed By I. Ramon I. Ramon I. Ramon I. Ramon Data Entry Date Sep-10-2019 Sep-10-2019 Days After First/Last Applic. 86 22 93 29 93 29 107 43
Trt Treatment Rate Appl No. Name Rate Unit Code Plot 29 30 31 32
1 Untreated Check 103 0,0 0,0 0,0 0,0 202 0,0 0,0 0,0 0,0 301 0,0 0,0 0,0 0,0 403 0,0 0,0 0,0 0,0
Mean = 0,0 0,0 0,0 0,0
2 Anolyte - AcquaAttiva 450 l/ha ABCDEFGH 101 0,0 0,0 0,0 0,0 203 0,0 0,0 0,0 0,0 302 0,0 0,0 0,0 0,0 401 0,0 0,0 0,0 0,0
Mean = 0,0 0,0 0,0 0,0 3 Amylo-X 2 kg/ha ABDF 102 0,0 0,0 0,0 0,0 Vacciplant 1 l/ha CE 201 0,0 0,0 0,0 0,0 Poltiglia Disperss 5 kg/ha GH 303 0,0 0,0 0,0 0,0
402 0,0 0,0 0,0 0,0 Mean = 0,0 0,0 0,0 0,0
Innovare e crescere insieme
PROTOCOL ID: 1062018G_B SATA TRIAL ID: 1062018G_B_01
Efficacy and selectivity of Anolyte (AcquAttiva®) to control Erwinia amylovora causing fireblight on
BIO pear
Pag. 23 di 38
Pagina 23 di 38
SATA S.r.l. – Strada Alessandria, 13 – 15044 Quargnento (AL) - Tel 0131219925 - Fax 0131219926 –
e-mail: [email protected] – web: www.satasrl.it
Pest Type D Disease D Disease D Disease Pest Code ERWIAM ERWIAM ERWIAM Pest Scientific Name Erwinia amylov> Erwinia amylov> Erwinia amylov> Pest Name Fire blight Fire blight Fire blight Crop Type, Code C PYUCO C PYUCO C PYUCO BBCH Scale BPOM BPOM BPOM Crop Scientific Name Pyrus communis Pyrus communis Pyrus communis Crop Name Common pear Common pear Common pear Rating Date Jul-12-2019 Jul-26-2019 Jul-26-2019 SE Group No. 13 35 14 Part Rated FRUATT P BUD P BUD P Rating Type COUINF COUINF COUINF Rating Unit NUMBER NUMBER NUMBER Sample Size 1 PLOT 1 PLOT 1 PLOT Number of Subsamples 1 1 1 Crop Stage Scale BBCH BBCH BBCH Crop Stage Majority 78 79 79 Crop Stage Minimum/Maximum 78 78 79 79 79 79 Assessed By I. Ramon I. Ramon I. Ramon Data Entry Date Sep-10-2019 Sep-10-2019 Days After First/Last Applic. 107 43 121 57 121 57
Trt Treatment Rate Appl No. Name Rate Unit Code Plot 33 34 35
1 Untreated Check 103 0,0 0,0 0,0 202 0,0 0,0 0,0 301 0,0 0,0 0,0 403 0,0 0,0 0,0
Mean = 0,0 0,0 0,0
2 Anolyte - AcquaAttiva 450 l/ha ABCDEFGH 101 0,0 0,0 0,0 203 0,0 0,0 0,0 302 0,0 0,0 0,0 401 0,0 0,0 0,0
Mean = 0,0 0,0 0,0 3 Amylo-X 2 kg/ha ABDF 102 0,0 0,0 0,0 Vacciplant 1 l/ha CE 201 0,0 0,0 0,0 Poltiglia Disperss 5 kg/ha GH 303 0,0 0,0 0,0
402 0,0 0,0 0,0 Mean = 0,0 0,0 0,0
Innovare e crescere insieme
PROTOCOL ID: 1062018G_B SATA TRIAL ID: 1062018G_B_01
Efficacy and selectivity of Anolyte (AcquAttiva®) to control Erwinia amylovora causing fireblight on
BIO pear
Pag. 24 di 38
Pagina 24 di 38
SATA S.r.l. – Strada Alessandria, 13 – 15044 Quargnento (AL) - Tel 0131219925 - Fax 0131219926 –
e-mail: [email protected] – web: www.satasrl.it
Pest Type D, Disease = Disease, such as a fungus, bacteria, or virus Pest Code ERWIAM, Erwinia amylovora, Fire blight = US Crop Type, Code C = EPPO species (Bayer) codes PYUCO, BPOM, Pyrus communis, Common pear = US Part Rated BUD = bud FLOCLU = flower cluster PLANT = plant FRUATT = fruit - attached P = Pest is Part Rated C = Crop is Part Rated Rating Type COUINF = count - infected PHYGEN = phytotoxicity - general / injury Rating Unit NUMBER = number % = percent PLOT = total plot Crop Stage Scale BBCH = BBCH uniform plant stages Crop Stage Majority 59 = Most flowers with petals forming a hollow ball 65 = Full flowerering: at least 50% of flowers open 69 = End of flowering: all petals fallen 71 = Fruit size up to 10mm; fruit fall after flowering 72 = Fruit size up to 20 mm 73 = Second fruit fall 74 = Fruit diameter up to 40 mm; fruit erect (T-stage: underside of fruit and stalk) 75 = Fruit about half final size 76 = Fruit about 60% of final size 77 = Fruit about 70% of final size 78 = Fruit about 80% of final size 79 = Fruit about 90% of final size Crop Stage Minimum/Maximum 60 = First flowers open 65 = Full flowerering: at least 50% of flowers open 69 = End of flowering: all petals fallen 71 = Fruit size up to 10mm; fruit fall after flowering 72 = Fruit size up to 20 mm 73 = Second fruit fall 74 = Fruit diameter up to 40 mm; fruit erect (T-stage: underside of fruit and stalk) 75 = Fruit about half final size 76 = Fruit about 60% of final size 77 = Fruit about 70% of final size 78 = Fruit about 80% of final size 79 = Fruit about 90% of final size 67 = Flowers fading: majority of petals fallen
Innovare e crescere insieme
PROTOCOL ID: 1062018G_B SATA TRIAL ID: 1062018G_B_01
Efficacy and selectivity of Anolyte (AcquAttiva®) to control Erwinia amylovora causing fireblight on
BIO pear
Pag. 25 di 38
Pagina 25 di 38
SATA S.r.l. – Strada Alessandria, 13 – 15044 Quargnento (AL) - Tel 0131219925 - Fax 0131219926 –
e-mail: [email protected] – web: www.satasrl.it
10.2 RAW WEATHER DATA
Closest Weather Station: Centallo Distance: 5 km
Moisture Min Max Avg Temp % Relative No. Date Total Unit Temp Temp Temp Unit Humidity
1. May-1-2019 0 mm 7,8 21,4 14,2 C 49
2. May-2-2019 2 mm 7,8 19,6 13,6 C 69 3. May-3-2019 8,4 mm 9,5 18,1 12,7 C 81
4. May-4-2019 3,4 mm 8,4 20,3 13,3 C 67
5. May-5-2019 3,8 mm 4,2 14,6 9,9 C 52
6. May-6-2019 0 mm 3,2 17,3 10,4 C 43 7. May-7-2019 0 mm 3,4 17,7 10,8 C 51
8. May-8-2019 6,2 mm 6,9 12,5 10 C 84
9. May-9-2019 0,2 mm 6,9 19,7 13 C 71
10. May-10-2019 0 mm 6,7 22,5 14,6 C 62 11. May-11-2019 2,2 mm 9,9 22,2 15,1 C 67
12. May-12-2019 0 mm 7,2 19,9 14,2 C 36
13. May-13-2019 0 mm 9,1 20,1 14,3 C 38
14. May-14-2019 0 mm 7,1 21,4 14,3 C 45 15. May-15-2019 0,4 mm 6,2 17,6 12,1 C 42
16. May-16-2019 0 mm 4 18,4 12 C 56
17. May-17-2019 11 mm 8,8 13,5 11,2 C 83
18. May-18-2019 24,4 mm 8,6 11,5 9,8 C 97 19. May-19-2019 6,2 mm 9,6 15,3 11,6 C 89
20. May-20-2019 0,2 mm 8 20,9 14 C 73
21. May-21-2019 0 mm 7,5 22,6 15,1 C 68
22. May-22-2019 6,6 mm 11,8 23,5 17,2 C 63 23. May-23-2019 0 mm 10,4 25,2 17,6 C 62
24. May-24-2019 0 mm 11,1 24,4 17 C 66
25. May-25-2019 41,6 mm 10,9 24,2 15,9 C 73
26. May-26-2019 31,8 mm 12,4 20 15 C 82 27. May-27-2019 6,2 mm 13,4 17,9 14,8 C 86
28. May-28-2019 3,6 mm 10,9 20,2 15 C 79
29. May-29-2019 0,2 mm 8,3 23 16,4 C 42
30. May-30-2019 0 mm 10,3 23,8 17,6 C 40 31. May-31-2019 0 mm 11,3 23,5 16,9 C 59
32. Jun-1-2019 0 mm 12 26,9 19,2 C 54
33. Jun-2-2019 0 mm 13,4 28,6 21,2 C 52
34. Jun-3-2019 0 mm 15,8 29,7 21,7 C 55 35. Jun-4-2019 0 mm 14,1 29,6 21,7 C 56
36. Jun-5-2019 0 mm 15,7 28,1 21,5 C 60
37. Jun-6-2019 0 mm 12,9 24,3 18,5 C 66 38. Jun-7-2019 0 mm 13,9 23,3 17,9 C 69
39. Jun-8-2019 0 mm 13,1 27,5 20,2 C 58
40. Jun-9-2019 1,6 mm 15,4 24,5 19,7 C 66
41. Jun-10-2019 0,8 mm 14,7 23,8 19,4 C 69 42. Jun-11-2019 0 mm 13 23,8 18,5 C 59
43. Jun-12-2019 0 mm 13 26,8 19,6 C 57
44. Jun-13-2019 0 mm 10,8 26,4 19,1 C 52
45. Jun-14-2019 0,6 mm 14,7 28,3 21,1 C 47 46. Jun-15-2019 0 mm 14,7 28,5 21,1 C 54
47. Jun-16-2019 0 mm 12,7 30,1 21,6 C 45
48. Jun-17-2019 0 mm 14,8 29,3 22,3 C 45
49. Jun-18-2019 0 mm 16,5 30,5 22,3 C 52 50. Jun-19-2019 0 mm 16 31,3 23,1 C 55
51. Jun-20-2019 0 mm 16 29,3 22,2 C 61
52. Jun-21-2019 0 mm 14,9 28,7 20,7 C 69
53. Jun-22-2019 13,4 mm 14,8 25,7 18,7 C 71 54. Jun-23-2019 0,2 mm 13,1 29 21,5 C 60
Innovare e crescere insieme
PROTOCOL ID: 1062018G_B SATA TRIAL ID: 1062018G_B_01
Efficacy and selectivity of Anolyte (AcquAttiva®) to control Erwinia amylovora causing fireblight on
BIO pear
Pag. 26 di 38
Pagina 26 di 38
SATA S.r.l. – Strada Alessandria, 13 – 15044 Quargnento (AL) - Tel 0131219925 - Fax 0131219926 –
e-mail: [email protected] – web: www.satasrl.it
55. Jun-24-2019 0 mm 17,3 32 24,7 C 65
56. Jun-25-2019 0 mm 21 33,1 26,6 C 67
57. Jun-26-2019 0 mm 20,6 34,4 27,5 C 72 58. Jun-27-2019 0 mm 21,2 38,3 29,2 C 64
59. Jun-28-2019 0 mm 21,6 37,9 29,2 C 50
60. Jun-29-2019 1,8 mm 22,4 31,5 26,1 C 58
61. Jun-30-2019 0 mm 20,9 32,7 26 C 62 62. Jul-1-2019 0,8 mm 19,2 34,5 25,7 C 52
63. Jul-2-2019 0 mm 16,9 32,8 24,5 C 58
64. Jul-3-2019 0,4 mm 15,9 31,4 23,4 C 50
65. Jul-4-2019 0 mm 16,6 30,8 23,8 C 57 66. Jul-5-2019 0 mm 17,9 33,6 25,6 C 65
67. Jul-6-2019 0 mm 18,2 33,1 25,5 C 63
68. Jul-7-2019 0 mm 19 35,9 25,6 C 69 69. Jul-8-2019 0 mm 15,3 33,6 23,7 C 54
70. Jul-9-2019 0,4 mm 14,5 25,2 20,1 C 78
71. Jul-10-2019 16,2 mm 14,8 26,1 20 C 85
72. Jul-11-2019 0 mm 15,1 24,3 19,6 C 86 73. Jul-12-2019 0 mm 14,2 30,6 21,7 C 69
74. Jul-13-2019 0 mm 11,2 31 21,5 C 60
75. Jul-14-2019 3 mm 14,8 28,1 20,3 C 69
76. Jul-15-2019 76,6 mm 9,8 16 12,5 C 95 77. Jul-16-2019 2,6 mm 10 28,3 18,7 C 71
78. Jul-17-2019 0 mm 11,8 29,3 20 C 73
79. Jul-18-2019 0 mm 13,5 28,5 20,4 C 75
80. Jul-19-2019 0 mm 14,7 29,4 21,3 C 75 81. Jul-20-2019 0 mm 16,8 30,2 22,6 C 73
82. Jul-21-2019 0 mm 15,9 31,2 22,8 C 79
83. Jul-22-2019 0 mm 16,9 32,1 23,8 C 77
84. Jul-23-2019 0 mm 17,5 33,2 24,2 C 77 85. Jul-24-2019 0 mm 17,1 34,2 24,6 C 77
86. Jul-25-2019 0 mm 18,4 35,5 26,2 C 70
87. Jul-26-2019 0 mm 19,2 34,5 26,1 C 71
88. Jul-27-2019 29 mm 12,7 28,1 19 C 92 89. Jul-28-2019 15,8 mm 12,4 29,6 20,3 C 83
90. Jul-29-2019 0 mm 14 31 22,1 C 79
91. Jul-30-2019 0 mm 15,5 32,1 23,3 C 78
92. Jul-31-2019 0 mm 14,9 30,8 22,5 C 79
Innovare e crescere insieme
PROTOCOL ID: 1062018G_B SATA TRIAL ID: 1062018G_B_01
Efficacy and selectivity of Anolyte (AcquAttiva®) to control Erwinia amylovora causing fireblight on
BIO pear
Pag. 27 di 38
Pagina 27 di 38
SATA S.r.l. – Strada Alessandria, 13 – 15044 Quargnento (AL) - Tel 0131219925 - Fax 0131219926 –
e-mail: [email protected] – web: www.satasrl.it
10.3 CHARTS
Chart 2 - Number of deseased bud at first assessment (30/05/2019)
Chart 3 - Number of deseased bud at second assessement (14/07/2019)
Innovare e crescere insieme
PROTOCOL ID: 1062018G_B SATA TRIAL ID: 1062018G_B_01
Efficacy and selectivity of Anolyte (AcquAttiva®) to control Erwinia amylovora causing fireblight on
BIO pear
Pag. 28 di 38
Pagina 28 di 38
SATA S.r.l. – Strada Alessandria, 13 – 15044 Quargnento (AL) - Tel 0131219925 - Fax 0131219926 –
e-mail: [email protected] – web: www.satasrl.it
10.4 STATISTICAL ANALYSIS Pest Type D Disease D Disease D Disease D Disease
Pest Code ERWIAM ERWIAM ERWIAM ERWIAM
Pest Scientific Name Erwinia amylov> Erwinia amylov> Erwinia amylov> Erwinia amylov>
Pest Name Fire blight Fire blight Fire blight Fire blight
Crop Type, Code C PYUCO C PYUCO C PYUCO C PYUCO C PYUCO
BBCH Scale BPOM BPOM BPOM BPOM BPOM
Crop Scientific Name Pyrus communis Pyrus communis Pyrus communis Pyrus communis Pyrus communis
Crop Name Common pear Common pear Common pear Common pear Common pear
Rating Date Mar-27-2019 Mar-27-2019 Apr-2-2019 Apr-2-2019 Apr-2-2019
SE Group No. 22 1 23 2 15
Part Rated BUD P FLOCLU P BUD P FLOCLU P PLANT C
Rating Type COUINF COUINF COUINF COUINF PHYGEN
Rating Unit NUMBER NUMBER NUMBER NUMBER %
Sample Size 1 PLOT 1 PLOT 1 PLOT 1 PLOT 1 PLOT
Number of Subsamples 1 1 1 1 1
Crop Stage Scale BBCH BBCH BBCH BBCH BBCH
Crop Stage Majority 59 59 65 65 65
Crop Stage Minimum/Maximum 60 60 60 60 65 65 65 65 65 65
Assessed By I. Ramon I. Ramon I. Ramon I. Ramon I. Ramon
Data Entry Date Sep-10-2019 Sep-10-2019 Sep-10-2019 Sep-10-2019
Days After First/Last Applic. 0 0 0 0 6 6 6 6 6 6
Trt Treatment Rate Appl No. Name Rate Unit Code 1* 2* 3* 4* 5*
1 Untreated Check 0,0 - 0,0 - 0,0 - 0,0 - 0,0 -
0,0 StDev 0,0 StDev 0,0 StDev 0,0 StDev 0,0 StDev
2 Anolyte - AcquaAttiva
450 l/ha ABCDEFGH 0,0 - 0,0 - 0,0 - 0,0 - 0,0 -
0,0 StDev 0,0 StDev 0,0 StDev 0,0 StDev 0,0 StDev
3 Amylo-X 2 kg/ha ABDF 0,0 - 0,0 - 0,0 - 0,0 - 0,0 -
Vacciplant 1 l/ha CE 0,0 StDev 0,0 StDev 0,0 StDev 0,0 StDev 0,0 StDev
Poltiglia Disperss 5 kg/ha GH
Tukey's HSD P=.05 . . . . .
Standard Deviation 0,00 0,00 0,00 0,00 0,00
CV 0,0 0,0 0,0 0,0 0,0
Levene's F 0,00 0,00 0,00 0,00 0,00
Levene's Prob(F) . . . . .
Skewness . . . . .
Kurtosis . . . . .
Replicate F 0,000 0,000 0,000 0,000 0,000
Replicate Prob(F) 1,0000 1,0000 1,0000 1,0000 1,0000
Treatment F 0,000 0,000 0,000 0,000 0,000
Treatment Prob(F) 1,0000 1,0000 1,0000 1,0000 1,0000
Innovare e crescere insieme
PROTOCOL ID: 1062018G_B SATA TRIAL ID: 1062018G_B_01
Efficacy and selectivity of Anolyte (AcquAttiva®) to control Erwinia amylovora causing fireblight on
BIO pear
Pag. 29 di 38
Pagina 29 di 38
SATA S.r.l. – Strada Alessandria, 13 – 15044 Quargnento (AL) - Tel 0131219925 - Fax 0131219926 –
e-mail: [email protected] – web: www.satasrl.it
Pest Type D Disease D Disease D Disease D Disease
Pest Code ERWIAM ERWIAM ERWIAM ERWIAM
Pest Scientific Name Erwinia amylov> Erwinia amylov> Erwinia amylov> Erwinia amylov>
Pest Name Fire blight Fire blight Fire blight Fire blight
Crop Type, Code C PYUCO C PYUCO C PYUCO C PYUCO C PYUCO
BBCH Scale BPOM BPOM BPOM BPOM BPOM
Crop Scientific Name Pyrus communis Pyrus communis Pyrus communis Pyrus communis Pyrus communis
Crop Name Common pear Common pear Common pear Common pear Common pear
Rating Date Apr-9-2019 Apr-9-2019 Apr-9-2019 Apr-17-2019 Apr-17-2019
SE Group No. 24 3 16 25 4
Part Rated BUD P FLOCLU P PLANT C BUD P FRUATT P
Rating Type COUINF COUINF PHYGEN COUINF COUINF
Rating Unit NUMBER NUMBER % NUMBER NUMBER
Sample Size 1 PLOT 1 PLOT 1 PLOT 1 PLOT 1 PLOT
Number of Subsamples 1 1 1 1 1
Crop Stage Scale BBCH BBCH BBCH BBCH BBCH
Crop Stage Majority 65 65 65 69 69
Crop Stage Minimum/Maximum 65 67 65 67 65 67 69 69 69 69
Assessed By I. Ramon I. Ramon I. Ramon I. Ramon I. Ramon
Data Entry Date Sep-10-2019 Sep-10-2019
Days After First/Last Applic. 13 7 13 7 13 7 21 8 21 8
Trt Treatment Rate Appl
No. Name Rate Unit Code 6* 7* 8* 9* 10*
1 Untreated Check 0,0 - 0,0 - 0,0 - 0,0 - 0,0 -
0,0 StDev 0,0 StDev 0,0 StDev 0,0 StDev 0,0 StDev
2 Anolyte - AcquaAttiva
450 l/ha ABCDEFGH 0,0 - 0,0 - 0,0 - 0,0 - 0,0 -
0,0 StDev 0,0 StDev 0,0 StDev 0,0 StDev 0,0 StDev
3 Amylo-X 2 kg/ha ABDF 0,0 - 0,0 - 0,0 - 0,0 - 0,0 -
Vacciplant 1 l/ha CE 0,0 StDev 0,0 StDev 0,0 StDev 0,0 StDev 0,0 StDev
Poltiglia Disperss 5 kg/ha GH
Tukey's HSD P=.05 . . . . .
Standard Deviation 0,00 0,00 0,00 0,00 0,00
CV 0,0 0,0 0,0 0,0 0,0
Levene's F 0,00 0,00 0,00 0,00 0,00
Levene's Prob(F) . . . . .
Skewness . . . . .
Kurtosis . . . . .
Replicate F 0,000 0,000 0,000 0,000 0,000
Replicate Prob(F) 1,0000 1,0000 1,0000 1,0000 1,0000
Treatment F 0,000 0,000 0,000 0,000 0,000
Treatment Prob(F) 1,0000 1,0000 1,0000 1,0000 1,0000
Innovare e crescere insieme
PROTOCOL ID: 1062018G_B SATA TRIAL ID: 1062018G_B_01
Efficacy and selectivity of Anolyte (AcquAttiva®) to control Erwinia amylovora causing fireblight on
BIO pear
Pag. 30 di 38
Pagina 30 di 38
SATA S.r.l. – Strada Alessandria, 13 – 15044 Quargnento (AL) - Tel 0131219925 - Fax 0131219926 –
e-mail: [email protected] – web: www.satasrl.it
Pest Type D Disease D Disease D Disease
Pest Code ERWIAM ERWIAM ERWIAM
Pest Scientific Name Erwinia amylov> Erwinia amylov> Erwinia amylov>
Pest Name Fire blight Fire blight Fire blight
Crop Type, Code C PYUCO C PYUCO C PYUCO C PYUCO C PYUCO
BBCH Scale BPOM BPOM BPOM BPOM BPOM
Crop Scientific Name Pyrus communis Pyrus communis Pyrus communis Pyrus communis Pyrus communis
Crop Name Common pear Common pear Common pear Common pear Common pear
Rating Date Apr-17-2019 Apr-25-2019 Apr-25-2019 Apr-25-2019 May-3-2019
SE Group No. 17 26 5 18 27
Part Rated PLANT C BUD P FRUATT P PLANT C BUD P
Rating Type PHYGEN COUINF COUINF PHYGEN COUINF
Rating Unit % NUMBER NUMBER % NUMBER
Sample Size 1 PLOT 1 PLOT 1 PLOT 1 PLOT 1 PLOT
Number of Subsamples 1 1 1 1 1
Crop Stage Scale BBCH BBCH BBCH BBCH BBCH
Crop Stage Majority 69 71 71 71 72
Crop Stage Minimum/Maximum 69 69 71 71 71 71 71 71 72 72
Assessed By I. Ramon I. Ramon I. Ramon I. Ramon I. Ramon
Data Entry Date Sep-10-2019 Sep-10-2019
Days After First/Last Applic. 21 8 29 8 29 8 29 8 37 8
Trt Treatment Rate Appl
No. Name Rate Unit Code 11* 12* 13* 14* 15*
1 Untreated Check 0,0 - 0,0 - 0,0 - 0,0 - 0,0 -
0,0 StDev 0,0 StDev 0,0 StDev 0,0 StDev 0,0 StDev
2 Anolyte - AcquaAttiva
450 l/ha ABCDEFGH 0,0 - 0,0 - 0,0 - 0,0 - 0,0 -
0,0 StDev 0,0 StDev 0,0 StDev 0,0 StDev 0,0 StDev
3 Amylo-X 2 kg/ha ABDF 0,0 - 0,0 - 0,0 - 0,0 - 0,0 -
Vacciplant 1 l/ha CE 0,0 StDev 0,0 StDev 0,0 StDev 0,0 StDev 0,0 StDev
Poltiglia Disperss 5 kg/ha GH
Tukey's HSD P=.05 . . . . .
Standard Deviation 0,00 0,00 0,00 0,00 0,00
CV 0,0 0,0 0,0 0,0 0,0
Levene's F 0,00 0,00 0,00 0,00 0,00
Levene's Prob(F) . . . . .
Skewness . . . . .
Kurtosis . . . . .
Replicate F 0,000 0,000 0,000 0,000 0,000
Replicate Prob(F) 1,0000 1,0000 1,0000 1,0000 1,0000
Treatment F 0,000 0,000 0,000 0,000 0,000
Treatment Prob(F) 1,0000 1,0000 1,0000 1,0000 1,0000
Innovare e crescere insieme
PROTOCOL ID: 1062018G_B SATA TRIAL ID: 1062018G_B_01
Efficacy and selectivity of Anolyte (AcquAttiva®) to control Erwinia amylovora causing fireblight on
BIO pear
Pag. 31 di 38
Pagina 31 di 38
SATA S.r.l. – Strada Alessandria, 13 – 15044 Quargnento (AL) - Tel 0131219925 - Fax 0131219926 –
e-mail: [email protected] – web: www.satasrl.it
Pest Type D Disease D Disease D Disease
Pest Code ERWIAM ERWIAM ERWIAM
Pest Scientific Name Erwinia amylov> Erwinia amylov> Erwinia amylov>
Pest Name Fire blight Fire blight Fire blight
Crop Type, Code C PYUCO C PYUCO C PYUCO C PYUCO C PYUCO
BBCH Scale BPOM BPOM BPOM BPOM BPOM
Crop Scientific Name Pyrus communis Pyrus communis Pyrus communis Pyrus communis Pyrus communis
Crop Name Common pear Common pear Common pear Common pear Common pear
Rating Date May-3-2019 May-3-2019 May-22-2019 May-22-2019 May-22-2019
SE Group No. 6 19 28 7 20
Part Rated FRUATT P PLANT C BUD P FRUATT P FRUATT P
Rating Type COUINF PHYGEN COUINF COUINF COUINF
Rating Unit NUMBER % NUMBER NUMBER NUMBER
Sample Size 1 PLOT 1 PLOT 1 PLOT 1 PLOT 1 PLOT
Number of Subsamples 1 1 1 1 1
Crop Stage Scale BBCH BBCH BBCH BBCH BBCH
Crop Stage Majority 72 72 73 73 73
Crop Stage Minimum/Maximum 72 72 72 72 73 73 73 73 73 73
Assessed By I. Ramon I. Ramon I. Ramon I. Ramon I. Ramon
Data Entry Date Sep-10-2019 Sep-10-2019
Days After First/Last Applic. 37 8 37 8 56 19 56 19 56 19
Trt Treatment Rate Appl
No. Name Rate Unit Code 16* 17* 18* 19* 20*
1 Untreated Check 0,0 - 0,0 - 0,0 - 0,0 - 0,0 -
0,0 StDev 0,0 StDev 0,0 StDev 0,0 StDev 0,0 StDev
2 Anolyte - AcquaAttiva
450 l/ha ABCDEFGH 0,0 - 0,0 - 0,0 - 0,0 - 0,0 -
0,0 StDev 0,0 StDev 0,0 StDev 0,0 StDev 0,0 StDev
3 Amylo-X 2 kg/ha ABDF 0,0 - 0,0 - 0,0 - 0,0 - 0,0 -
Vacciplant 1 l/ha CE 0,0 StDev 0,0 StDev 0,0 StDev 0,0 StDev 0,0 StDev
Poltiglia Disperss 5 kg/ha GH
Tukey's HSD P=.05 . . . . .
Standard Deviation 0,00 0,00 0,00 0,00 0,00
CV 0,0 0,0 0,0 0,0 0,0
Levene's F 0,00 0,00 0,00 0,00 0,00
Levene's Prob(F) . . . . .
Skewness . . . . .
Kurtosis . . . . .
Replicate F 0,000 0,000 0,000 0,000 0,000
Replicate Prob(F) 1,0000 1,0000 1,0000 1,0000 1,0000
Treatment F 0,000 0,000 0,000 0,000 0,000
Treatment Prob(F) 1,0000 1,0000 1,0000 1,0000 1,0000
Innovare e crescere insieme
PROTOCOL ID: 1062018G_B SATA TRIAL ID: 1062018G_B_01
Efficacy and selectivity of Anolyte (AcquAttiva®) to control Erwinia amylovora causing fireblight on
BIO pear
Pag. 32 di 38
Pagina 32 di 38
SATA S.r.l. – Strada Alessandria, 13 – 15044 Quargnento (AL) - Tel 0131219925 - Fax 0131219926 –
e-mail: [email protected] – web: www.satasrl.it
Pest Type D Disease D Disease D Disease D Disease
Pest Code ERWIAM ERWIAM ERWIAM ERWIAM
Pest Scientific Name Erwinia amylov> Erwinia amylov> Erwinia amylov> Erwinia amylov>
Pest Name Fire blight Fire blight Fire blight Fire blight
Crop Type, Code C PYUCO C PYUCO C PYUCO C PYUCO C PYUCO
BBCH Scale BPOM BPOM BPOM BPOM BPOM
Crop Scientific Name Pyrus communis Pyrus communis Pyrus communis Pyrus communis Pyrus communis
Crop Name Common pear Common pear Common pear Common pear Common pear
Rating Date May-30-2019 May-30-2019 May-30-2019 Jun-7-2019 Jun-7-2019
SE Group No. 29 8 21 30 9
Part Rated BUD P FRUATT P BUD P BUD P FRUATT P
Rating Type COUINF COUINF COUINF COUINF COUINF
Rating Unit NUMBER NUMBER NUMBER NUMBER NUMBER
Sample Size 1 PLOT 1 PLOT 1 PLOT 1 PLOT 1 PLOT
Number of Subsamples 1 1 1 1 1
Crop Stage Scale BBCH BBCH BBCH BBCH BBCH
Crop Stage Majority 74 74 74 75 75
Crop Stage Minimum/Maximum 74 74 74 74 74 74 75 75 75 75
Assessed By I. Ramon I. Ramon I. Ramon I. Ramon I. Ramon
Data Entry Date Sep-10-2019 Sep-10-2019 Sep-10-2019 Sep-10-2019 Sep-10-2019
Days After First/Last Applic. 64 8 64 8 64 8 72 8 72 8
Trt Treatment Rate Appl
No. Name Rate Unit Code 21* 22* 23* 24* 25*
1 Untreated Check 12,3 a 0,0 - 0,0 - 0,0 - 0,0 -
5,9 StDev 0,0 StDev 0,0 StDev 0,0 StDev 0,0 StDev
2 Anolyte - AcquaAttiva
450 l/ha ABCDEFGH 6,5 b 0,0 - 0,0 - 0,0 - 0,0 -
2,1 StDev 0,0 StDev 0,0 StDev 0,0 StDev 0,0 StDev
3 Amylo-X 2 kg/ha ABDF 5,0 b 0,0 - 0,0 - 0,0 - 0,0 -
Vacciplant 1 l/ha CE 2,4 StDev 0,0 StDev 0,0 StDev 0,0 StDev 0,0 StDev
Poltiglia Disperss 5 kg/ha GH
Tukey's HSD P=.05 5,70 . . . .
Standard Deviation 2,63 0,00 0,00 0,00 0,00
CV 33,22 0,0 0,0 0,0 0,0
Levene's F 1,901 0,00 0,00 0,00 0,00
Levene's Prob(F) 0,205 . . . .
Skewness 1,5577* . . . .
Kurtosis 3,0128* . . . .
Replicate F 4,542 0,000 0,000 0,000 0,000
Replicate Prob(F) 0,0548 1,0000 1,0000 1,0000 1,0000
Treatment F 8,470 0,000 0,000 0,000 0,000
Treatment Prob(F) 0,0179 1,0000 1,0000 1,0000 1,0000
Innovare e crescere insieme
PROTOCOL ID: 1062018G_B SATA TRIAL ID: 1062018G_B_01
Efficacy and selectivity of Anolyte (AcquAttiva®) to control Erwinia amylovora causing fireblight on
BIO pear
Pag. 33 di 38
Pagina 33 di 38
SATA S.r.l. – Strada Alessandria, 13 – 15044 Quargnento (AL) - Tel 0131219925 - Fax 0131219926 –
e-mail: [email protected] – web: www.satasrl.it
Pest Type D Disease D Disease D Disease D Disease D Disease
Pest Code ERWIAM ERWIAM ERWIAM ERWIAM ERWIAM
Pest Scientific Name Erwinia amylov> Erwinia amylov> Erwinia amylov> Erwinia amylov> Erwinia amylov>
Pest Name Fire blight Fire blight Fire blight Fire blight Fire blight
Crop Type, Code C PYUCO C PYUCO C PYUCO C PYUCO C PYUCO
BBCH Scale BPOM BPOM BPOM BPOM BPOM
Crop Scientific Name Pyrus communis Pyrus communis Pyrus communis Pyrus communis Pyrus communis
Crop Name Common pear Common pear Common pear Common pear Common pear
Rating Date Jun-14-2019 Jun-14-2019 Jun-21-2019 Jun-21-2019 Jun-28-2019
SE Group No. 31 10 32 11 33
Part Rated BUD P FRUATT P BUD P FRUATT P BUD P
Rating Type COUINF COUINF COUINF COUINF COUINF
Rating Unit NUMBER NUMBER NUMBER NUMBER NUMBER
Sample Size 1 PLOT 1 PLOT 1 PLOT 1 PLOT 1 PLOT
Number of Subsamples 1 1 1 1 1
Crop Stage Scale BBCH BBCH BBCH BBCH BBCH
Crop Stage Majority 76 76 77 77 78
Crop Stage Minimum/Maximum 76 76 76 76 77 77 77 77 78 78
Assessed By I. Ramon I. Ramon I. Ramon I. Ramon I. Ramon
Data Entry Date Nov-28-2019 Sep-10-2019 Sep-10-2019 Sep-10-2019
Days After First/Last Applic. 79 15 79 15 86 22 86 22 93 29
Trt Treatment Rate Appl
No. Name Rate Unit Code 26* 27* 28* 29* 30*
1 Untreated Check 16,8 a 0,0 - 0,0 - 0,0 - 0,0 -
1,7 StDev 0,0 StDev 0,0 StDev 0,0 StDev 0,0 StDev
2 Anolyte - AcquaAttiva
450 l/ha ABCDEFGH 11,0 b 0,0 - 0,0 - 0,0 - 0,0 -
1,6 StDev 0,0 StDev 0,0 StDev 0,0 StDev 0,0 StDev
3 Amylo-X 2 kg/ha ABDF 4,3 c 0,0 - 0,0 - 0,0 - 0,0 -
Vacciplant 1 l/ha CE 2,2 StDev 0,0 StDev 0,0 StDev 0,0 StDev 0,0 StDev
Poltiglia Disperss 5 kg/ha GH
Tukey's HSD P=.05 4,47 . . . .
Standard Deviation 2,06 0,00 0,00 0,00 0,00
CV 19,33 0,0 0,0 0,0 0,0
Levene's F 0,043 0,00 0,00 0,00 0,00
Levene's Prob(F) 0,958 . . . .
Skewness -0,1834 . . . .
Kurtosis -1,0001 . . . .
Replicate F 0,471 0,000 0,000 0,000 0,000
Replicate Prob(F) 0,7138 1,0000 1,0000 1,0000 1,0000
Treatment F 36,843 0,000 0,000 0,000 0,000
Treatment Prob(F) 0,0004 1,0000 1,0000 1,0000 1,0000
Innovare e crescere insieme
PROTOCOL ID: 1062018G_B SATA TRIAL ID: 1062018G_B_01
Efficacy and selectivity of Anolyte (AcquAttiva®) to control Erwinia amylovora causing fireblight on
BIO pear
Pag. 34 di 38
Pagina 34 di 38
SATA S.r.l. – Strada Alessandria, 13 – 15044 Quargnento (AL) - Tel 0131219925 - Fax 0131219926 –
e-mail: [email protected] – web: www.satasrl.it
Pest Type D Disease D Disease D Disease D Disease D Disease
Pest Code ERWIAM ERWIAM ERWIAM ERWIAM ERWIAM
Pest Scientific Name Erwinia amylov> Erwinia amylov> Erwinia amylov> Erwinia amylov> Erwinia amylov>
Pest Name Fire blight Fire blight Fire blight Fire blight Fire blight
Crop Type, Code C PYUCO C PYUCO C PYUCO C PYUCO C PYUCO
BBCH Scale BPOM BPOM BPOM BPOM BPOM
Crop Scientific Name Pyrus communis Pyrus communis Pyrus communis Pyrus communis Pyrus communis
Crop Name Common pear Common pear Common pear Common pear Common pear
Rating Date Jun-28-2019 Jul-12-2019 Jul-12-2019 Jul-26-2019 Jul-26-2019
SE Group No. 12 34 13 35 14
Part Rated FRUATT P BUD P FRUATT P BUD P BUD P
Rating Type COUINF COUINF COUINF COUINF COUINF
Rating Unit NUMBER NUMBER NUMBER NUMBER NUMBER
Sample Size 1 PLOT 1 PLOT 1 PLOT 1 PLOT 1 PLOT
Number of Subsamples 1 1 1 1 1
Crop Stage Scale BBCH BBCH BBCH BBCH BBCH
Crop Stage Majority 78 78 78 79 79
Crop Stage Minimum/Maximum 78 78 78 78 78 78 79 79 79 79
Assessed By I. Ramon I. Ramon I. Ramon I. Ramon I. Ramon
Data Entry Date Sep-10-2019 Sep-10-2019 Sep-10-2019
Days After First/Last Applic. 93 29 107 43 107 43 121 57 121 57
Trt Treatment Rate Appl
No. Name Rate Unit Code 31* 32* 33* 34* 35*
1 Untreated Check 0,0 - 0,0 - 0,0 - 0,0 - 0,0 -
0,0 StDev 0,0 StDev 0,0 StDev 0,0 StDev 0,0 StDev
2 Anolyte - AcquaAttiva
450 l/ha ABCDEFGH 0,0 - 0,0 - 0,0 - 0,0 - 0,0 -
0,0 StDev 0,0 StDev 0,0 StDev 0,0 StDev 0,0 StDev
3 Amylo-X 2 kg/ha ABDF 0,0 - 0,0 - 0,0 - 0,0 - 0,0 -
Vacciplant 1 l/ha CE 0,0 StDev 0,0 StDev 0,0 StDev 0,0 StDev 0,0 StDev
Poltiglia Disperss 5 kg/ha GH
Tukey's HSD P=.05 . . . . .
Standard Deviation 0,00 0,00 0,00 0,00 0,00
CV 0,0 0,0 0,0 0,0 0,0
Levene's F 0,00 0,00 0,00 0,00 0,00
Levene's Prob(F) . . . . .
Skewness . . . . .
Kurtosis . . . . .
Replicate F 0,000 0,000 0,000 0,000 0,000
Replicate Prob(F) 1,0000 1,0000 1,0000 1,0000 1,0000
Treatment F 0,000 0,000 0,000 0,000 0,000
Treatment Prob(F) 1,0000 1,0000 1,0000 1,0000 1,0000
Innovare e crescere insieme
PROTOCOL ID: 1062018G_B SATA TRIAL ID: 1062018G_B_01
Efficacy and selectivity of Anolyte (AcquAttiva®) to control Erwinia amylovora causing fireblight on
BIO pear
Pag. 35 di 38
Pagina 35 di 38
SATA S.r.l. – Strada Alessandria, 13 – 15044 Quargnento (AL) - Tel 0131219925 - Fax 0131219926 –
e-mail: [email protected] – web: www.satasrl.it
Pest Type D, Disease = Disease, such as a fungus, bacteria, or virus Pest Code ERWIAM, Erwinia amylovora, Fire blight = US Crop Type, Code C = EPPO species (Bayer) codes PYUCO, BPOM, Pyrus communis, Common pear = US Part Rated BUD = bud FLOCLU = flower cluster PLANT = plant FRUATT = fruit - attached P = Pest is Part Rated C = Crop is Part Rated Rating Type COUINF = count - infected PHYGEN = phytotoxicity - general / injury Rating Unit NUMBER = number % = percent PLOT = total plot Crop Stage Scale BBCH = BBCH uniform plant stages Crop Stage Majority 59 = Most flowers with petals forming a hollow ball 65 = Full flowerering: at least 50% of flowers open 69 = End of flowering: all petals fallen 71 = Fruit size up to 10mm; fruit fall after flowering 72 = Fruit size up to 20 mm 73 = Second fruit fall 74 = Fruit diameter up to 40 mm; fruit erect (T-stage: underside of fruit and stalk) 75 = Fruit about half final size 76 = Fruit about 60% of final size 77 = Fruit about 70% of final size 78 = Fruit about 80% of final size 79 = Fruit about 90% of final size Crop Stage Minimum/Maximum 60 = First flowers open 65 = Full flowerering: at least 50% of flowers open 69 = End of flowering: all petals fallen 71 = Fruit size up to 10mm; fruit fall after flowering 72 = Fruit size up to 20 mm 73 = Second fruit fall 74 = Fruit diameter up to 40 mm; fruit erect (T-stage: underside of fruit and stalk) 75 = Fruit about half final size 76 = Fruit about 60% of final size 77 = Fruit about 70% of final size 78 = Fruit about 80% of final size 79 = Fruit about 90% of final size 67 = Flowers fading: majority of petals fallen
Innovare e crescere insieme
PROTOCOL ID: 1062018G_B SATA TRIAL ID: 1062018G_B_01
Efficacy and selectivity of Anolyte (AcquAttiva®) to control Erwinia amylovora causing fireblight on
BIO pear
Pag. 36 di 38
Pagina 36 di 38
SATA S.r.l. – Strada Alessandria, 13 – 15044 Quargnento (AL) - Tel 0131219925 - Fax 0131219926 –
e-mail: [email protected] – web: www.satasrl.it
10.5 TRIAL SITE LOCATION
Innovare e crescere insieme
PROTOCOL ID: 1062018G_B SATA TRIAL ID: 1062018G_B_01
Efficacy and selectivity of Anolyte (AcquAttiva®) to control Erwinia amylovora causing fireblight on
BIO pear
Pag. 37 di 38
Pagina 37 di 38
SATA S.r.l. – Strada Alessandria, 13 – 15044 Quargnento (AL) - Tel 0131219925 - Fax 0131219926 –
e-mail: [email protected] – web: www.satasrl.it
Innovare e crescere insieme
PROTOCOL ID: 1062018G_B SATA TRIAL ID: 1062018G_B_01
Efficacy and selectivity of Anolyte (AcquAttiva®) to control Erwinia amylovora causing fireblight on
BIO pear
Pag. 38 di 38
Pagina 38 di 38
SATA S.r.l. – Strada Alessandria, 13 – 15044 Quargnento (AL) - Tel 0131219925 - Fax 0131219926 –
e-mail: [email protected] – web: www.satasrl.it