Fido B2 & Fido C Series Briefing - emitec industrial€¦ · Acetylcholine Choline + Acetic acid Cholinesterase enzyme . Proprietary - 31Company Confidential ©2016 FLIR Systems Inc

Fido B2 & Fido C Series Briefing

Robert J. Sovesky

February 2016

Proprietary - Company Confidential ©2016 FLIR Systems Inc. Information and equipment described herein may require US Government authorization for export purposes. Diversion contrary to US law is prohibited. 2

FIDO B2 Biological Detection


Reliable & Fast: Detects all four classes of bio-agents at concentrations less than 100 ACPLA in under 1 minute with 0 start up time (immediate alarm capability); High confidence trigger with low false alarm rates (less than 1 per month). User-Friendly: Designed to run continuously in the background with minimal interaction. Can be integrated with most HVAC systems. Low maintenance and consumables. Field-Proven: Most mature and widely deployed bio-trigger device on the market today (~1,000 sensors deployed; millions of hours of continuous run-time in relevant operational environments). Affordable: Detector only triggers the collector when there is a high probability of a biological agent attack, significantly reducing cost of ownership

Fido® B2 (formerly IBAC) - Key Benefits


How does Fido B2 - work?


Elastic Scattering

Fluorescence Emission

Laser Light

Particle Detection based on principal of Mie Scattering:

Laser Excitation:

Aerosol particles pass through UV laser beam

Scattered light is collected by an optical detector

Elastic Scattering light measures:

Particle Concentration

determined from number of light pulses

Particle size

Determined from amplitude of light pulses

Fluorescence Emission:

Biological particles absorb the UV light and may

emit light at longer wavelengths than the excitation

source.

Fluorescent light is collected by a 2nd optical

detector

Laser Excitation

Fluorescence Emission

Laser Diode Excitation Source

Color Spectrum

How does it work? Optical Scattering & Fluorescence


Detector Air Intake

Battery Compartment

Power Input

On/Off Switch

Sample Collector Wireless Radio

Antenna and GPS

Ethernet and Serial Communication

Visual Indicator Lights

M42 Alarm Posts

Collapsible Handle

Fido B2 - Hardware Overview


Ready for Transport • No Battery Compartment • No Antenna • No Extender Stack • Collapsed Handle

Operational - Line Power • No Battery Compartment • Antenna and Extender

Stack installed • Handle Upright

Operational – Battery Power • Running on Battery Power • Antenna and Extender

Stack installed • Handle Upright

Deployment Configurations


Communicator Real-Time Plotting

Communicator LT Software:

Controls and operates all functions of the

sensor

Real-time particle data

Alarms

Diagnostic data

Stores all real-time data onto connected

computer; downloads stored memory card

files

How does the user interact with the Fido B2?


Standard Tools: • Needle Nose Pliers • 25 inch-lbs Torque Wrench • 5/16 wrench • Phillips Screwdriver • 3/32 Allen Wrench • Nozzle Cleaning Wire

Description Recommended Frequency Performed By Replace Inlet Screen 6 Months Onsite

Clean Air Inlet Nozzle Pressure Fault Alarm, or 6 months Onsite

Aerosol Calibration and Optics Cleaning

1 Year By Manufacturer Only

Replace Internal HEPA Filter 3 Years By Manufacturer Only

Optional Tool Kit

Low Maintenance Requirements


Aerosol Sample Collection


• Aerosol Collectors are designed to collect a physical sample of airborne particles

• Particles impact onto a collection surface.

• Particles are then extracted into a liquid to be analyzed by common bio-identification equipment such as PCR devices or Hand-Held Assay test strips.

• Fido B2 only detects the presence of biological material – it does not identify the specific organism.

What is Aerosol Sample Collection?


• DFU = Dry Filter Unit

• A DFU Filter is placed on top of the rotating fan. The collector acts as a vacuum to impact particles onto the filter surface.

• Following sampling, the filter disk is removed and placed into a conical vial with liquid buffer for particle extraction.

• Advantages compared to C100 Collector: • 75% smaller size of collection stack

• Consumables are cheaper

• No cleaning steps required

DFU Collector - Dry Sampling


• Wet sampling is based on rotating impactor technology

• The rotating impactor is a disk with vanes which is rotated at high speed, both entraining air into the system and impacting particles onto the surface.

• Following sampling, the rotating disk is manually rinsed to remove the collected particles from the surface into a small liquid volume (~ 5.0 mL in a sample vial).

• Advantages compared to DFU Collector: • Allows for both Wet and Dry sampling methods

• 50% higher air flow rate

• Wet sampling eliminates one less particle extraction step

C100 Collector – Wet Sampling


Deployment


Fido B2 With Sample Collector

PCR Identifier (RAZOR EX from BioFire)

Liquid

Sample

Seconds Minutes < 1 Hour

Stage 1: Real-Time

Bio-Detection

Stage 2: Triggered Sample

Collection

Stage 3: On-Site Confirmation

and Identification

Biological Detection System: 3 Stage Process


Long-term, Fixed Location, Continuous Monitoring Applications

• Building / Facility Protection

• Mass transit

• Critical Infrastructure

• High profile public sites

Short-term, Tactical Response Missions

• Sporting / Entertainment events

• Political events

• Perimeter monitoring around military forward operating bases

• Vehicle and UAV applications

Indoor / Outdoor Deployment Options


• Array of multiple sensors can be quickly deployed together for rapid response

• Setup within a facility or around a perimeter for wide area monitoring

• Wireless communication transmits all data back to a central base station computer

• Sensors are small and mobile enough to be grouped together in three transport cases

• A kit of 5x sensors, 1x laptop, and all accessories can be contained in three 1620 Pelican cases

Kit of 5 sensors and accessories in 3 transport cases

Fido® B2 - Rapid Deployment Setup


ABQ AIRPORT TEST

without monitoring with monitoring


ABQ AIRPORT TEST



Vehicle Integration


Fido B2 and BioXC Located Within Vehicle or Container Detector and Collector Stacks Remotely Located on top of Roof

Detector

Stack

Collector

Stack

Fido B2

Sensor BioXC

Sensor

Air

Flow

Collector

Motor

Cable

Liquid

Tubing

Sensors located

within a vehicle.

Possibly within a

Glove Box.

Air Intake Stacks

located on top of

vehicle roof

Rheinmetall

4x4


FIDO C SERIES Chemical Agent Detection


How do nerve agents work?

AChE enzyme turnover number = 25,000 / second!!

acetylcholinesterase

acetylcholine

• Organophosphate nerve agents attack a key neural enzyme called acetylcholinesterase (AChE)

• Acetylcholine is a neurotransmitter that is used to transmit impulses to activate muscles

• AChE enzyme removes acetylcholine to stop & control process, to reset muscle


• Organophosphate nerve agents attack a key neural enzyme called acetylcholinesterase (AChE)

• Acetylcholine is a neurotransmitter that is used to transmit impulses to activate muscles

• AChE enzyme removes acetylcholine to stop & control process, to reset muscle

• Nerve agents inhibit/block AChE • AChE stops working causing a

buildup of acetylcholine, ultimately causing spasm of muscles, eventually leading to respiratory failure

acetylcholinesterase

AChE enzyme blocked = Build up of acetylcholine

acetylcholine

nerve agent

X

How do nerve agents work?


Utilizing AChE enzyme as sensor

• Enzyme reaction causing a change of pH

– Change of pH can be measured by a pH electrode or by color when using pH sensitive dyes

ACID drop in pH

Acetylcholine Choline + Acetic acid Cholinesterase enzyme


Utilizing AChE enzyme as sensor (change of pH)

• Enzyme inhibition causes no change of pH

NO ACID

Chem agent

Acetylcholine Choline + Acetic acid

Phosphorylated Acetylcholinesterase enzyme blocked *INHIBITED*

X X


Chemical Agent Detection & Disclosure

FLIR enzyme based product families enable the user to detect chemical warfare agent (CWA) and/or Toxic Industrial Chemical (TIC) contamination on surfaces or in air

Fido® C1 Chemical Agent Detection Kit

– Low-cost pens to check surface contamination & visible chemical spills

Fido C2 Chemical Agent Disclosure Spray

– Spray for detecting and mapping CWA contamination over large surfaces

– Available in handheld, man-portable & wide area scales


Fido C1 - Chemical Agent Detection


Customizable Kit Containing 6 Sensors

• Useful in bulk chemical identification or detection of trace levels of hazards on surfaces pre/post-decon

• Chemical class-based detection

– Nerve agent (G-, V-series), Blood agent (AC), Blister agent (HD), Strong acid/base, Aldehydes, Oxidizers

• Low false positive rate

• Sensitivity: Microgram-level sensitivity

• Easy to use with simple instruction card – Activated like glow sticks

• Training version available for operational exercises


Optional Sampling Tool for Bulk

• Sampler is used when environment is likely to be incompatible with direct use of sensor – Bulk materials

– Discoloring environment

– Harsh chemical background

• Sampler consists of sterile swab and a dispensable reservoir with a dilute buffer solution – After sampling, contents are simply dispensed directly into pen

cap

– Dilutes sample by roughly 100-fold

– Carries sufficient volume for multiple pens and for sample retention


Key Benefits & Mission Space of Fido C1

• Key Benefits – Low false positive rate – Microgram-level sensitivity – Excellent orthogonal detection technique for validating

responses from other detection equipment – Two-year shelf life (


Tactics, Techniques, Procedures (TTPs)


Comparison with M8/M9 paper

• Fido C1 pens detect trace (ug-) quantities of agents on surfaces through a simple color change • Fido C1 pens are compatible with samples in water and on surfaces • Fido C1 pens have operational utility • Fido C1 pens are suitable for decon verification • Fido C1 pens are suitable for identifying contamination on sensitive equipment • Fido C1 pens are suitable for locating low / no volatility agent contamination on surfaces • Fido C1 pens have broad sample compatibility and resistance to potential environmental

interference problematic to other sensors

Gasoline Insect repellent Sunscreen


Capabilities/Limitations

• Detectable Agents: – Nerve agents (G- & V-series) - Oxidizers

– Blister agents (HD) - Acid/caustics

– Blood agents (AC & CK) - Aldehydes

• No significant temperature effects: – Storage for 2 weeks at -30 degrees C, 60 degrees C, and cycled

temps between hot and cold has been tested

– 2 years at room temperature

• Operational temps of 0 or 45 degrees C (32 to 113 F)

• CAD pen can be used for evidence collection and sampling


Fido C2 - Agent Disclosure Spray


Fido C2 - Agent Disclosure Spray

• Colorimetric detection of CWA

– Map contamination

– Reduce logistical burden for decon

• Disclosure Sprays are surface sensors

• Nerve (V- & G-series), Blister (HD) & Training

• Handheld, man-portable and cart-based

• Detect sub-microgram of agents on surfaces


Fido C2 saves Decon Resources

• Estimate of decontamination requirements for 10 vehicles per FM 3-11-5

Decon Station

Water (gal) Men

Needed Time (man-hr) Decon (lb) Cost ($)

Primary

Wash 2500 6-8 5 0 0

Decon 200-600 12 10 1000-3000 ~$40,000

Contact Time 0 3 5-10 0 0

Rinse 2000 4-6 5 0 0

Check 0 2 10-20 0 0

Totals 4700-5100 27-31 35-50 1000-3000 ~$40,000

Estimate of decontamination requirements for 10 vehicles per FM 3-11-5


Fido C2 saves Decon Resources

• Estimate of decontamination requirements for 10 vehicles per FM 3-11-5

Decon Station

Water (gal) Men

Needed Time (man-hr) Decon (lb) Cost ($)

Primary

Wash 150 2 4 0 ~$2,500*

Decon 50-200 3-4 2-3 250-1000 ~$10,000

Contact Time 0 3 5-10 0 0

Rinse 500-600 2 2 0 0

Check 0 2 2 0 ~$2,500*

Totals 4700-5100 27-31 35-50 1000-3000 ~$40,000

Totals with Fido

C2 being used 700-950 12-13 15-21 250-1000 ~$15,000 *

Savings ~83% ~57% ~58% ~69% ~63% * Includes additional costs of Fido C2 disclosure spray


Training with Fido C2

• Same functionality as live agent versions

• Reacts to urea, a benign simulant, on surface

• Trainings Scenarios

– Release mapping & contamination avoidance

– Equipment decon

– Personnel decon


NEW! - Fluorescent Additive for Fido C2

• Fido C2 + Fluorescent Additive + UV Light

• Stronger visible detections on challenging surfaces

– Wheel wells

– Dark carpet

– Black surfaces

– Sand

– Bad light conditions

Fido C2 with Fluorescent Additive under ambient light

Fido C2 with Fluorescent Additive with UV light


NEW! - Forensic Option of Fido C2

• No visible residue

• Forensic Fido C2 + UV Light Source

• Forensic detection on building interiors and public areas

Forensic Fido C2 after application

Detection event of Forensic Fido C2 with UV light source


Fido C2 - Key Features

• Sensitivity: Unmatched sensitivity compared with other handheld detection technologies

• Relatively ‘Low-tech’ and does not require special operators

• Utility for recon and decon

• Efficient mapping without intensive use of personnel or resources

• Ability to Improve Speed, Efficiency, and Reliability of Decontamination by enabling warfighter to see and locate contaminated areas


Fido C Series - Current Customers & Support

• Developed with funding from agencies whose mission is to protect the warfighter

• Performance has been extensively validated via live agent testing

– Edgewood Chemical Biological Command (ECBC) and Battelle

– Various international test labs

• Entire Programs have been built and fielded around disclosure technology

• Fielded by 20th Support Command (CBRNE) and WMD Civil Support Teams

Documents

Fido B2 & Fido C Series Briefing - emitec industrial€¦ · Acetylcholine Choline + Acetic acid Cholinesterase enzyme . Proprietary - 31Company Confidential ©2016 FLIR Systems Inc