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FFPE RNA/DNA Purification Plus Kit Sequential Isolation and Purification of Total RNA and Genomic DNA from FFPE Complete column purification - Separate fractionation of RNA and DNA Reduce variability - RNA and DNA are isolated from a single sample with no splitting of the lysate Isolate a diversity of RNA species - All sizes of RNA are isolated, from large mRNA down to microRNA Isolate high quality RNA and DNA - The purified RNA and DNA are of the highest quality and can be used in a number of downstream applications 0 2 4 6 Competitor Norgen RNA Yeild (μg) RNA Yield 0 10 20 30 Competitor Norgen DNA Yield (μg) DNA Yield 0.0 1.0 2.0 3.0 260:280 260:230 Ratio Value RNA Ratio Competitor Norgen 0.0 1.0 2.0 3.0 260:280 260:230 Ratio Value DNA Ratio Competitor Norgen A C B D Figure 1. Superior Recovery of High Quality RNA and DNA from FFPE Spleen Tissues. Norgen’s FFPE RNA/DNA Purification Plus Kit isolates FFPE RNA and DNA that exceeds the yield of competitors. Cycle 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 10 10 10 10 10 10 10 10 10 10 Cycle 0 5 10 15 20 25 30 35 10 10 10 10 10 10 10 10 10 Threshold 5S rRNA (DNA Detection) A B Cycle 5 10 15 20 25 30 35 10 10 10 10 10 10 10 10 C miR-21 (miRNA Detection) beta-Actin (mRNA Detection) Norgen Competitor Figure 2. Recovery of High Quality DNA and True Total RNA including microRNA from Hamster Spleen. Genomic DNA isolated using Norgen’s FFPE RNA/DNA Purification Plus Kit is of high quality with effective qPCR amplification. NORGEN BIOTEK CORP. The Sample Preparation Experts T 1-866-NORGENB T 905-227-8848 F 905-227-1061 E [email protected] www.norgenbiotek.com ISO 13485:2003, ISO 9001:2008, ISO 15189:2012 Registered

FFPE RNA/DNA Purification Plus Kit Plus v5.pdfFFPE RNA/DNA Purification Plus Kit Sequential Isolation and Purification of Total RNA and Genomic DNA from FFPE Complete column purification

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Page 1: FFPE RNA/DNA Purification Plus Kit Plus v5.pdfFFPE RNA/DNA Purification Plus Kit Sequential Isolation and Purification of Total RNA and Genomic DNA from FFPE Complete column purification

FFPE RNA/DNA Purification Plus KitSequential Isolation and Purification of Total RNA and Genomic DNA from FFPE

Complete column purification - Separate fractionation of RNA and DNA

Reduce variability - RNA and DNA are isolated from a single sample with no splitting of the lysate

Isolate a diversity of RNA species - All sizes of RNA are isolated, from large mRNA down to microRNA

Isolate high quality RNA and DNA - The purified RNA and DNA are of the highest quality and can be used in a number of downstream applications

0

2

4

6

Competitor Norgen

RNA

Yeild

(μg)

RNA Yield

0

10

20

30

Competitor Norgen

DN

A Yi

eld

(μg)

DNA Yield

0.0

1.0

2.0

3.0

260:280 260:230

Ratio

Val

ue

RNA Ratio Competitor

Norgen

0.0

1.0

2.0

3.0

260:280 260:230

Ratio

Val

ue

DNA Ratio Competitor

Norgen

A

C

B

D

Figure 1. Superior Recovery of High Quality RNA and DNA from FFPE Spleen Tissues. Norgen’s FFPE RNA/DNA Purification Plus Kit isolates FFPE RNA and DNA that exceeds the yield of competitors.

Cycle3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28

Nor

m. F

luor

o.

-0.410

-0.610

-0.810

-1.010

-1.210

-1.410

-1.610

-1.810

-2.010

-2.210

Cycle0 5 10 15 20 25 30 35

Nor

m. F

luor

o.

-0.210

-0.410

-0.610

-0.810

-1.010

-1.210

-1.410

-1.610

-1.810

Threshold

5S rRNA(DNADetection)

A B

Cycle5 10 15 20 25 30 35

Nor

m. F

luor

o.

-0.210

-0.410

-0.610

-0.810

-1.010

-1.210

-1.410

-1.610

C

miR-21(miRNADetection)

beta-Actin(mRNADetection)

Norgen

Competitor

Figure 2. Recovery of High Quality DNA and True Total RNA including microRNA from Hamster Spleen. Genomic DNA isolated using Norgen’s FFPE RNA/DNA Purification Plus Kit is of high quality with effective qPCR amplification.

NORGENBIOTEK CORP.The Sample Preparation Experts

T 1-866-NORGENBT 905-227-8848

F 905-227-1061E [email protected]

www.norgenbiotek.comISO 13485:2003, ISO 9001:2008,ISO 15189:2012 Registered

Page 2: FFPE RNA/DNA Purification Plus Kit Plus v5.pdfFFPE RNA/DNA Purification Plus Kit Sequential Isolation and Purification of Total RNA and Genomic DNA from FFPE Complete column purification

Manufactured in our ISO accredited facility.

NORGENBIOTEK CORP.The Sample Preparation Experts

Publication Title Authors Journal Year

MicroRNA-34c-5p is related to recurrence in laryngeal squa-mous cell carcinoma

Massimo Re, Artan Çeka, Corrado Rubini, Luigi Ferrante, Antonio Zizzi, Federico M. Gioacchini, Michele Tulli, Liana Spazzafumo, Stefano Sellari-Franceschini, Antonio

TheLaryngoscope 2015

In-Vivo Fusion of Human Cancer and Hamster Stromal Cells Permanently Transduces and Transcribes Human DNA

In-Vivo Fusion of Human Cancer and Hamster Stromal Cells Permanently Transduces and Transcribes Human DNA

PLOS ONE 2014

Description Specification

Binding Capacity of RNA Per Column Up to 35 μg

Binding Capacity of DNA Per Column Up to 10 μg

Maximum Loading Volume Per Spin Column 600 μL

Size of RNA Purified All sizes, including < 200 nt

Maximum Amount of Starting Material 4 sections <20 μM thick10 mg of unsectioned block

Description Prep Size Cat. Number

FFPE RNA/DNA Purification Plus Kit 50 preps 54300

FFPE RNA Purification Kit (Spin column/96-well) 50 preps / 96-well 25300 / 25400

FFPE DNA Purification Kit 50 preps 47400

Select Publications

Kit Specifications (54300)

Ordering Information

v5.0