Upload
dangliem
View
235
Download
0
Embed Size (px)
Citation preview
Elemental Scientific
FAST for prep3 enhances laboratory productivity by adding FAST uptake, washout, and high throughput capabilities to the prep3 autodilution system. Syngistix control of a 7 port valve and three independent microperistaltic pumps is used to perform accurate and precise inline autodilution. Rapid mixing of samples or standards with diluent and internal standard in a fl uoropolymer sample fl ow path provides minimal memory effects.
FAST for prep3Automated Inline Dilution System for PerkinElmer Optima
FAST for prep3 Automated Inline Dilution System with Autosampler
2prep3-87A prep3 with
2DX Autosampler
FX-57C FASTX Complete
Features
• Triple low-pulsing microperistaltic pump
- P1 = Carrier
- P2 = Diluent
- P3 = Internal standard
• FAST injection
• In-valve mixing
Benefits
• Automatic calibration from stock standard solution(s)
• Automatic sample dilution
• Automatic over-range dilution
• Automatic internal standard out-of-range dilution
• Analyze samples from undiluted to up to 25x diluted
• Fully supported by PerkinElmer Syngistix software
Ni Calibration Curve
Figure 1. Two calibration standards, 0.5 ppm and 10 ppm, were used to create the calibration curve from 0.025 ppm to 10 ppm.
Elemental Scientific
Figure 2. Defining stock standard concentration and autosampler location in the Syngistix software is performed in the calibration tab. In this example, autocalibration from two stock standards creates a calibration curve from 0.025 ppm to 10 ppm for Fe, Mg, Mn, V, and 2.5ppm to 50ppm for Ca. Syngistix uses the stock standard locations, concentrations, and dilutions to autocalibrate the Optima.
Autocalibration of multiple stock standards to create calibration curve from 0.025 ppm to 10 ppm
Figure 3. Syngistix flexibility allows autodilution factors to be the same for all samples (example A) or sample specific (example B). FAST for prep3 eliminates offline manual dilution of samples.
Automatic predefined dilution of samples to eliminate manual offline dilution of samples
A B
Elemental Scientific
Autodilution of over-range samplesfor over-range analytes
Figure 4. The upper concentration range for one or more analytes can be defined for automatic dilution of over-range samples. Set the upper limit (A) and the over-range dilution factor (B).
Figure 5. Syngistix monitors the internal standard and FAST for prep3 automatically dilutes out-of-range samples further for reanalysis.
Automatic dilution for out-of-range internal standard
B
A
Range
Dilute Further
Elemental Scientific
16099
0%20%40%60%80%
100%120%140%160%180%200%
1 11 21 31 41 51 61 71 81 91 101
111
121
131
141
151
161
171
181
191
201
211
221
% R
ecov
ery
Sample No
% Recovery – 1 ppm Y Internal Standard
Y 371.029
% Recovery - 1 ppm Y Internal Standard
Figure 6. 9+ hour internal standard recovery. Average recovery: 98.5%.
0
20
40
60
80
100
120
Al Mg Ni Co Mn Ba Be Zn B
% R
ecov
ery
Analyte
% Average Recovery For Analytes 200+Average % Recoveries for Analytes
Figure 7. Average % recovery for over 200 samples of 0.5 ppm & 10 ppm analyte concentrations. Average analyte recovery was 100.8%.
3. After the instrument’s analysis has completed, the loop is rinsed and the nebulizer line and spray chamber are washed by the carrier, diluent, and internal standard
Sample
Vacuum
1. Vaccum load sample into loop while carrier, diluent, and internal standard rinse the nebulizer line
7: IS / Diluent
2: Carrier
3: Nebulizer
2. Peristaltic pumps adjust flow rates to perform the dilution and add internal standard inline
7: IS / Diluent
2: Carrier
3: Nebulizer
Rinse
Vacuum
7: IS / Diluent
2: Carrier
3: Nebulizer
FAST for prep3 Flow Path
98.5% Internal Standard Recovery
For more information on prep3, please visit www.prep3.com or contact us at [email protected]
Elemental Scientific7277 World Communications Dr.Omaha, NE 68122 USA Phone: 1.402.991.7800
FAST for prep3(Just add a FASTX Complete to any prep3 System)
P/N DescriptionFX-57C FAST X Complete
prep3 SystemsP/N Description
prep3-87A prep3 Inline Autodilution System for existing DX autosampler user
2prep3-87A prep3 2DX Inline Autodilution System
4prep3-87A prep3 4DX Inline Autodilution System