MFSH MFSH 20032003

PRESS F1F1 FOR GUIDEANCE

Enterobacteria, Vibrio, Campylobacter & Enterobacteria, Vibrio, Campylobacter & Pseudomonas Pseudomonas

EnterobacteriaEnterobacteria 33Station 1: Gram stain of Enterobacteria 4Station 2: NLF + LF on Mc Conkey 5Station 3: Klebsiella on Mc Conkey 6Station 4: E.coli on Mc Conkey 7Station 5: Pure LF & Pure NLF on both Mc Conkey & DCA 8Station 6: Proteus on Blood agar 9Station 7: Selenite F broth 10Station 8: Indole production 11Station 9: H2S Production 12Station 10: Widal test 13Station 11: Treatment & diseases 14VibrioVibrio 1515Station 11: Gram stain of Vibrio cholera 16Station 12: Growth on TCBS 17Station 13: Growth on TCBS & alkaline peptone water 18CampylobacterCampylobacter 1919Station 14: Gram stain of Campylobacter 20Station 15: Urease Production 21PseudomonasPseudomonas 2222Station 16: Growth on Nutrient agar 23Station 17: Sensitivity test 24Station 18: Growth on blood media 25Station 20: Treatment and diseases 26

E.g.E.g.

E.coliE.coli

KlebsiellaKlebsiella

ProteusProteus

ShigellaShigella

SalmonellaSalmonella

and others.and others.

Non lactose Non lactose fermentersfermenters

Station 1Station 1

NLF= non lactose fermenters

LF= lactose fermenters

NLF

LF

Station 2Station 2

Large mucoid colonies Large mucoid colonies (pinkish / reddish)(pinkish / reddish)

Station 3Station 3

Pink coloniesPink colonies

Station 4Station 4

PinkishPinkish

PinkishPinkish

PalePale

PalePale

LF (e.g. E-coli & Klepsilla)LF (e.g. E-coli & Klepsilla)

N.B. it is E-coli here N.B. it is E-coli here

““Shape of colonies”Shape of colonies”

LFLF

NLF (e.g. Salmonella, Shigella)NLF (e.g. Salmonella, Shigella)

NLFNLF

Station 5Station 5

Station 6Station 6

Samonella Samonella

andand

ShigellaShigella

Station 7Station 7

Station 8Station 8

Blacke.g. shigella

Station 9Station 9

1/10 1/20 1/40 ……… 1/640

+ test+ test- test- test

1/10 1/20 1/401/40

++ --

1/10 1/20 1/40 1/80 1/160 1/3201/320++ --

There is more than 4 folds increase in the titer of antibodies against Salmonella typhi “320/40= 8”, this means acute infection.

Station 10Station 10

Escherichis coli

Infection:-

1- urinary tract infection

2- diarrhea

Treatment:-

requires antibiotic testing, but most common are

1-”Ampicilin”

2-”Aminglycosides”

Klebsiella

Infection:-

urinary tract infection

Treatment:-

beta lactamase producing

“Aminglycosides” is used

Proteus

Infection:-

urinary tract infection, by the urease the urea is split to produce ammonia “alkaline urine”

Salmonella

Infection:-

1- typhoid fever

2- food poising “meat, poultry & egg” diarrhea

This usually happens when people eat in public places “Pakistan, Sudan & Egypt”

Treatment:-

In enteric fever “chloramphenicol”, if resistant “ciprofloxacilin”

Shigilla

Infection:-

dysenteriae (enterotoxin, cytotoxic & neurotoxic) bloody, mucoid & small stools with fever

Treatment:-

Fluid replacement & “Ampicillin”

Station 11Station 11

E.g.E.g.

Vibrio CholeraeVibrio Cholerae

Station 12Station 12

Station 13Station 13

aureus

Mentioned Mentioned previouslypreviously

Clear Clear

TurbidTurbid

Station 14Station 14

)vibrio(

E.g.E.g.

Campylobacter jejuni Campylobacter jejuni

Station 15Station 15

Helicobacter pyloriHelicobacter pylori

Station 16Station 16

No pigment

Station 17Station 17

SensitiveSensitive

SensitiveSensitive

Pseudomonas is resistant to Pseudomonas is resistant to many antibiotics “but many antibiotics “but sensitive to amino glycosides sensitive to amino glycosides (e.g. gentamicin) and some (e.g. gentamicin) and some others” others”

Station 18Station 18

Note that Pseudomonas is strictly aerobic

Green orange

Station 19Station 19

Vibrio cholera

Infection:-

cholera (exotoxin persistent and excessive secretion of isotonic fluid by intestinal mucosa)

Treatment:-

“Erythromycin”

Campylobacter jejuni

Infection:-

Food poisoning “Chicken” diarrhea.

Treatment:-

“Erythromycin”

Helicobacter pylori

Infection:-

Gastric and duodenal ulcerations

Treatment:-

Triple therapy: “Omeprazole” + “Amoxycilin” + “Metronidazole”

Quadruple therapy: Same + proton pump inhibitors

Pseudomonas

Infection:-

Gastric and duodenal ulcerations

Treatment:-

“Erythromycin”, “Amoxycilin”

Station 20Station 20

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THANXTHANX

M. F. ShaheenM. F. Shaheen 2003 2003