FACULTY OF ALLIED MEDICAL SCIENCE

HAEMOSTATIC COMPONENTS

ILOS

By the end of this lecture, the students will be able to recognize;

What is Fresh frozen plasma and cryoprecipitate.

CONTENT

FRESH FROZEN PLASMA (FFP)

Prepared from whole blood donation and frozen within 6 – 8 hours .

It is stored at – 18o c or colder for up to 12 months.

It contains: 91% water – 7% protein – 2% carbohydrate.

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FRESH FROZEN PLASMA (FFP)

Indications

To treat prolonged PT or PTT

Vitamin K deficiency and liver disease

Dilutional coagulopathy

Protein S, Protein C or Antithronbin deficiency.

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FRESH FROZEN PLASMA (FFP)

Thawing

It should be thawed at temperature 30-37oc in a water bath.

The unit should be wrapped in a plastic bag.

Thawed FFP should be transfused immediately or within 12 hrs or stored between 1-6oc for no more than 12 hours.

It should not be refrozen.

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FRESH FROZEN PLASMA (FFP)

Administration

1. The same ABO as the protein.

2. Given via a filter over 1 – 1.5 hours.

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CRYOPRECIPITATE

Obtained from FFP and concentrated to 10 -20 ml.

It contains F VIII, (80 – 120 Units), v WF, 250 mg fibrinogen, 20 – 30% of factor XIII & fibronectin.

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CRYOPRECIPITATE

Indications

Factor VIII deficiency (Haemophilia A).

Type II VW disease.

Type I VW disease not responding to DDAVP.

Factor XIII deficiency.

Congenital fibrinogen abnormality.

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CRYOPRECIPITATE

Indications

When fibrinogen is ≤ 100 mg.

Control of bleeding in uremic or hepatic patients.

For preparation of fibrin glue: Topical use in orphopedic, ENT, dental, cardiac or neurological procedures.

Reversal of warfarin overdose.

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CRYOPRECIPITATE

Thawing

It should be thawed at 37 0 c immediately before use and the pack should NOT be refrozen.

Fibrinogen dose: 8 – 10 bags supply 2 g fibrinogen.

Infusion

Filter at rate of 1 – 2 ml/min.

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CRYOPRECIPITATE

Cryoprecipirate represents the cryoglobulin fraction of the plasma.

It contains F VIII, VWF, fibrinogen, fibronectin and F XIII.

It is used primarily for the treatment of Haemophilia A patients.

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PLATELET CONCENTRATES

Volume: 50 – 70 ml/unit provide about 5000 platelets. Kept at 20 – 22 0 c in a shaker for a maximum of 5 days.

Should be infused within 4 hours, administered with filter

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PROTHROMBIN COMPLEX

This component contains prothrombin (F II), factor VII, F IX and factor X.

It is lyophylized and virus-inactivated to reduce the risk of transmitting infections.

Indications Haemophilia B (F IX deficiency). Serious coumarin overdosage together with

Vitamin K1 Coagulation defects due to fulminant hepatitis

or liver insufficiency.

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FEIBA AND AUTOPLEX

These are some activated prothrombin complex preparations used for treatment of Haemophilia A patients with inhibitors.

These should be given with caution, as overuse may cause thrombosis and DIC.

Currently, there are factors IX and F VII concentrates for TR of Haemophilia B.

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FIBRIN GLUE

It is formed by the reaction of cryo (fibrinogen) + thrombin as they are applied topically.

Advantage Good hemostasis in different solid organ

bleeding. Prevents post-operative bleeding. Efficient in the presence of coagulation

defect and bacterial contamination.

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STUDY QUESTION

Mention the indication of cryopercipitate.

ASSIGNMENTS

Complication of blood transfusion

محمد مصطفى محمد

THANK YOU