Extraction of DNA From Extraction of DNA From Wheat GermWheat Germ

(Your Name)(Your Name)Cell BiologyCell Biology

Period 6Period 6November 6, 2006November 6, 2006

What is DNA?What is DNA?

DNA is a nucleic acid called DNA is a nucleic acid called deoxyribonucleic acid. deoxyribonucleic acid.

Its structure is a double helixIts structure is a double helix It contains all of an organisms hereditary It contains all of an organisms hereditary

informationinformation…………

What is Wheat Germ?What is Wheat Germ?

Explain where wheat germ comes from Explain where wheat germ comes from

Explain why it contains a large amount of Explain why it contains a large amount of DNADNA

What does it look like?What does it look like?

Extraction of DNA Experiment Extraction of DNA Experiment Supplies ListSupplies List

A list of the supplies and equipment that A list of the supplies and equipment that you need to conduct your experiment to you need to conduct your experiment to extract DNA from the raw wheat germextract DNA from the raw wheat germ

Explanation of what each supply does in Explanation of what each supply does in your protocolyour protocol

Supplies ListSupplies List

Test TubeTest Tube Raw Wheat GermRaw Wheat Germ Warm WaterWarm Water Non-Iodized SaltNon-Iodized Salt Detergent (Dawn) Detergent (Dawn) Ice Cold EthanolIce Cold Ethanol Meat Tenderizer (Adolph’s)Meat Tenderizer (Adolph’s) Wooden Splint or Glass Stirring RodWooden Splint or Glass Stirring Rod Baking SodaBaking Soda

Extraction ProtocolExtraction Protocol

Step by step procedure that you will follow Step by step procedure that you will follow to extract the DNA from raw wheat germto extract the DNA from raw wheat germ

Make sure the font is big enough for Make sure the font is big enough for viewers to seeviewers to see

Use more than one page if your protocol is Use more than one page if your protocol is longlong

Extraction ProtocolExtraction Protocol

1.1. Weigh out 1.5 g of raw Wheat Germ and Weigh out 1.5 g of raw Wheat Germ and place in test tubeplace in test tube

2.2. Add enough warm water to cover and Add enough warm water to cover and dissolve the wheat germ dissolve the wheat germ

3.3. Add 5ml of liquid detergent to test tubeAdd 5ml of liquid detergent to test tube4.4. Add 3g of meat tenderizer to test tubeAdd 3g of meat tenderizer to test tube5.5. Add a small amount of baking soda Add a small amount of baking soda

solution to the test tubesolution to the test tube6.6. Add 1g of salt to test tubeAdd 1g of salt to test tube

Extraction ProtocolExtraction Protocol

1.1. Pour 10ml of ice cold ethanol into the test tube.Pour 10ml of ice cold ethanol into the test tube.2.2. Let the test tube stand for a few minutes to Let the test tube stand for a few minutes to

allow the DNA to precipitate from solution.allow the DNA to precipitate from solution.3.3. DNA will form as a white, cottony substance in DNA will form as a white, cottony substance in

the solution.the solution.4.4. Collect the DNA material by spooling it up with Collect the DNA material by spooling it up with

the wooden splint.the wooden splint.5.5. Let the DNA dry on a paper towel.Let the DNA dry on a paper towel.

What are the Results of the DNA What are the Results of the DNA Extraction?Extraction?

Thank You Page (Sources)Thank You Page (Sources)